NM_172069.4;PLEKHH1L
100μg liquid plasmid(Instantly):$163
| name | pEnCMV-EGFP-2×Linker-PLEKHH2(human)(1-702aa)(921-1493aa)-SV40-Neo | another name | apEnCMV-EGFP-2×Linker-PLEKHH2(human)(1-702aa)(921-1493aa)-SV40-Neo |
| Insert Size (bp) | 4482bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGGC GGCGGAGGGA GTGGAGGAGG GGGAAGCAAG CTTATGGCAG AGCTTTCTGA GCCAGAGGGA CCAGTAGATT GGAAGGAACG ATGTGTAGCT CTGGAGTCCC AACTCATGAA ATTTAGAGTT CAAGCAAGCA AGATACGAGA GCTTTTAGCA GAGAAGATGC AACAGCTTGA GAGACAAGTT ATTGATGCTG AACGTCAAGC AGAAAAAGCT TTTCAACAGG TACAAGTTAT GGAAGATAAA TTAAAAGCAG CTAATATTCA AACCAGTGAA TCAGAGACAA GATTATATAA TAAGTGTCAA GATCTGGAGT CGCTAATACA GGAAAAAGAT GACGTCATTC AAAACTTGGA ATTGCAACTT GAAGAGCAGA AACAAATAAG AATACAAGAA GCTAAAATAA TAGAAGAGAA AGCAGCTAAG ATAAAAGAAT GGGTAACAGT TAAGTTAAAT GAGCTGGAAT TGGAGAATCA GAATCTTCGT TTGATCAACC AAAACCAAAC TGAAGAGATA AGAACAATGC AGTCAAAACT ACAAGAAGTT CAAGGAAAGA AGTCATCCAC TGTCTCTACA CTAAAGCTTT CGGAAGGCCA GCGCCTGAGC AGTTTGACCT TTGGGTGCTT TTTATCTCGA GCAAGGAGTC CTCCTCAAGT AGTAAAATCT GAGGAAATGA GCAAGATATC ATCGAAAGAA CCTGAGTTCA CTGAAGGAAA AGACATGGAA GAAATGGAAA TTCCAGAAAA GTCTGTTGAT AACCAAGTTC TAGAAAACAA CAGAGGCCAG AGAACATTGC ATCAAACCCC TTGTGGCTCA GAACAGAATC GGAAAACAAG AACAAGCTTT GCCACAGATG GTGGCATCTC CCAGAATTCT GGGGCTCCTG TGAGTGACTG GAGCTCTGAT GAGGAAGACG GGAGCAAAGG AAGATCCAAG TCCAGATGCA CATCCACCCT CTCCAGTCAC ACATCTGAGG AAGGGGTCCA GTGTAGCAGG ATGGGAAGTG AAATGTATCT GACAGCATCT GATGACAGCA GCTCTATATT TGAGGAAGAG ACTTTTGGCA TAAAGAGACC AGAACACAAG AAGCTATATT CTTGGCAGCA GGAGGCACAG TGGAAAGCTC TAAATAGTCC TCTTGGAAAG GGAAATTCTG AATTAAGTAA AAAGGAACAA GATAGTTCCT CGGATGAACT GAATAAAAAA TTTCAATCCC AGAGACTCGA TTATTCATCT TCATCGAGTG AAGCCAACAC CCCAAGCCCT ATTTTGACCC CAGCTTTAAT GCCAAAGCAT CCTAACTCAC TCTCTGGAAA AGGAACACAA TTAGTGCCTT CATCACACCT GCCACCCCCA AAGTTAAGGA TTCCTAATGT TTTCAGTATA AGTGTAGCAC TAGCCAAAAG GCACTTAAGC CAGCCACAGT TAAGCTCTGA CAGGATGTTT GGTACAAATA GAAACGCTAT AAGCATGATA CGACCACTGA GACCTCAGGA AACTGATCTT GATCTAGTTG ATGGAGACAG TACAGAAGTT TTAGAGAATA TGGACACGAG TTGTGATGAT GGATTATTTT CCTATGACTC CTTGGACTCT CCAAATTCAG ATGACCAGGA ACACTGTGAC TCAGCAAAGA AGGTGGCATA CAGCAAACCT CCAACTCCTC CCCTGCACCG TTTTCCTTCT TGGGAAAGCA GAATTTATGC TGTAGCCAAA TCAGGTATTC GAATGTCTGA GGCCTTCAAT ATGGAGAGTG TTAATAAAAA TTCTGCTGCA ACCCTTTCCT ATACTACATC AGGACTTTAT ACATCTCTGA TATACAAGAA CATGACCACC CCAGTGTATA CAACTTTGAA GGGGAAGGCG ACCCAAATAA GTAGCAGCCC TTTCCTGGAT GACTCATCTG GGTCAGAGGA AGAAGACAGC TCCAGATCCA GCTCCCGGAC GTCAGAGTCA GACTCACGCA GTAGGAGTGG GCCAGGCAGC CCCAGAGCCA TGAAACGAGG TGTGTCTCTC TCCTCTGTGG CTTCTGAAAG TGATTATGCT ATTCCTCCTG ATGCTTACTC CACAGACACG GAGTACTCAC AGCCAGAGCA GAAGCTCCCA AAAACTTGCT CATCTTCCAG TGATAATGGG AAAAATGAAA ACAATGTAAA CGTTGGATCT GAATTTGAAC AACTGGTTTG CAAATTGCTA AATATAGACG GGGAGCCTTC CTCTCAGATA TGGAGACACC CCACTTTGTG TCACAGTAAA GAAGGAATCA TTTCCCCTCT GACAACTCTA CCTTCCGAAG CCCTGCAGAC AGAAGCTATT AAATTATTTA AGACCTGCCA GCTTTTTATA AATGCTGCAG TTGACTCTCC TGCAATTGAT TACCACATAT CTTTAGCCCA GAGTGCTTTG CAAATCTGCC TGACACATCC TGAGCTGCAG AATGAAATTT GCTGTCAGCT TATTAAACAG ACAAGACGAA GACAGCCACA GAATCAACCA GGACCATTGC AGGGCTGGCA GCTCTTGGCA CTCTGCGTTG GGCTCTTCCT TCCCCATCAT CCTTTCCTGT GGCTCCTCAG GCTTCACCTA AAGAGGAATG CAGATTCCAG GACAGAATTT GGAAAATATG CCATTTACTG CCAGCGTTGT GTAGAAAGAA CGCAACAAAA TGGTGACAGA GAAGCAAGAC CCTCAAGGAT GGAAATTCTT TCAACTCTTC TCCGAAACCC TTATCACCAT TCTTTGCCCT TTAGTATACC TGTGCACTTC ATGAATGGGA TATACCAGGT AGTTGGTTTT GACGCATCTA CCACAGTGGA AGAATTTTTG AATACTTTGA ACCAGGACAC AGGAATGAGG AAACCAGCGC AGTCTGGATT TGCGTTGTTC ACTGACGATC CTTCTGGCAG AGATTTAGAG CATTGTCTTC AAGGAAACAT CAAGATTTGT GACATTATTT CCAAATGGGA ACAGGCTTCC AAAGAACAGC AGCCTGGAAA ATGTGAAGGT ACAAGGACTG TTCGTCTGAC ATACAAAAAC AGACTATATT TCTCAGTGCA AGCTCGTGGA GAGACTGATA GAGAAAAGTT GCTGTTAATG TATCAGACAA ATGATCAAAT CATAAATGGA CTTTTTCCTC TGAACAAAGA TCTGGCATTA GAAATGGCAG CTCTTTTATC TCAGGTAGAG ATTGGAGATT TTGAAAGACC TTTCTCAACT CCAGCAGGGC ATGTTACCAA TCAGTGCAAA GTGAATCAAA CTCTAAAGCA AGTCATAGAG AAATTTTATC CTAAAAGGTA TAGAGATGGC TGTTCTGAAG AGCAGTTAAG GCAGCTTTGC CAGCGACTTT CAACCAGATG GATGGCCCTC CGGGGACACA GTGCTGCTGA CTGTGTGCGC ATTTATTTGA CAGTAGCCAG GAAGTGGCCA TTCTTTGGTG CCAAGTTGTT TCTTGCAAAA CCCATAACTC CATCATCACT TGGAAGTACT TTCTTGTGGC TGGCTGTACA TGAGGATGGT TTAAGCCTCT TAGAATACAA CTCCATGAGG TTAATAGTCA GCTATGTGTA CAAGAGTCTA ATGACCTTTG GAGGCTATCA AGATGATTTT ATGGTAGTCA TTAACAATAC ACATTCAAAG GACAAACCAA CAGAGAAATT ACTTTTTGCC ATGGCAAAAC CCAAGATTCT TGAAATCACT CTTTTGATCG CCAGTTACAT AAACAACTTC CATCAGCAAA AGGCAGCATT TCACCACCTC TCTGCTCCAG CACTGCTCTC AGCCCAGACC CGGGGACCCC AAGCCAGAAT GATGGGAAGC CAGCCTCTTC TGTCAAGCAG CAGACCGACC AAAGGCCCCA CCTTACTCTG AGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAACGCGTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTCCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCTAGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCCT CAGGTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCATG CAT
