pLVX-EnCMV-Flag-HA-BRIX1(human)-PGK-Puro
$84.00
In stock
SKU
PL57961
NM_018321.4;BRIX; BXDC2
100μg liquid plasmid(Instantly):$105
| name | pLVX-EnCMV-Flag-HA-BRIX1(human)-PGK-Puro | another name | apLVX-EnCMV-Flag-HA-BRIX1(human)-PGK-Puro |
| Insert Size (bp) | 1062bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA GGTTAGGAAC AGAGAGACAG CAGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCCGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GCGCGCCAGT CCTCCGATAG ACTGCGTCGC CCGGGTACCC GTATTCCCAA TAAAGCCTCT TGCTGTTTGC ATCCGAATCG TGGACTCGCT GATCCTTGGG AGGGTCTCCT CAGATTGATT GACTGCCCAC CTCGGGGGTC TTTCATTTGG AGGTTCCACC GAGATTTGGA GACCCCTGCC CAGGGACCAC CGACCCCCCC GCCGGGAGGT AAGCTGGCCA GCGGTCGTTT CGTGTCTGTC TCTGTCTTTG TGCGTGTTTG TGCCGGCATC TAATGTTTGC GCCTGCGTCT GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT GGTGGAACTG ACGAGTTCTG AACACCCGGC CGCAACCCTG GGAGACGTCC CAGGGACTTT GGGGGCCGTT TTTGTGGCCC GACCTGAGGA AGGGAGTCGA TGTGGAATCC GACCCCGTCA GGATATGTGG TTCTGGTAGG AGACGAGAAC CTAAAACAGT TCCCGCCTCC GTCTGAATTT TTGCTTTCGG TTTGGAACCG AAGCCGCGCG TCTTGTCTGC TGCAGCGCTG CAGCATCGTT CTGTGTTGTC TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATTAGGG CCAGACTGTT ACCACTCCCT TAAGTTTGAC CTTAGGTCAC TGGAAAGATG TCGAGCGGAT CGCTCACAAC CAGTCGGTAG ATGTCAAGAA GAGACGTTGG GTTACCTTCT GCTCTGCAGA ATGGCCAACC TTTAACGTCG GATGGCCGCG AGACGGCACC TTTAACCGAG ACCTCATCAC CCAGGTTAAG ATCAAGGTCT TTTCACCTGG CCCGCATGGA CACCCAGACC AGGTCCCCTA CATCGTGACC TGGGAAGCCT TGGCTTTTGA CCCCCCTCCC TGGGTCAAGC CCTTTGTACA CCCTAAGCCT CCGCCTCCTC TTCCTCCATC CGCCCCGTCT CTCCCCCTTG AACCTCCTCG TTCGACCCCG CCTCGATCCT CCCTTTATCC AGCCCTCACT CCTTCTCTAG GCGCCGGAAT TAGATCCTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCCCTA TCAGTGATAG AGATCTCCCT ATCAGTGATA GAGATCGTCG ACGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGGGACC GATCCAGCCT CCGGGGATCT GCCACCATGG ACTACAAGGA TGACGATGAC AAGCTCGATG GAGGATACCC ATACGATGTT CCAGATTACG CTGCTCGAGG TTATCAAACA AGTTTGTACA AAAAAGTTGG CATGGCGGCA ACCAAGAGGA AACGGCGTGG AGGCTTTGCA GTTCAGGCGA AGAAGCCAAA AAGAAACGAA ATAGATGCGG AGCCGCCAGC TAAGCGGCAC GCCACAGCAG AGGAGGTGGA GGAAGAAGAG AGGGACCGGA TCCCAGGCCC CGTTTGCAAG GGAAAGTGGA AAAATAAGGA ACGGATTCTC ATCTTTTCTT CCAGAGGAAT AAATTTTAGA ACAAGACATT TAATGCAGGA CTTGAGAATG TTGATGCCTC ATTCTAAAGC AGATACTAAA ATGGATCGTA AGGATAAGCT ATTTGTGATT AACGAGGTTT GTGAAATGAA GAACTGTAAT AAATGCATCT ATTTTGAAGC TAAGAAAAAA CAGGATCTCT ATATGTGGCT TTCAAATTCA CCTCACGGAC CATCTGCTAA ATTCCTTGTT CAAAATATTC ATACCCTCGC TGAACTGAAG ATGACTGGAA ACTGTTTGAA AGGTTCTCGG CCCCTTTTGT CTTTTGACCC TGCTTTTGAT GAATTACCAC ATTATGCTTT GTTAAAAGAA CTCTTAATTC AGATCTTTAG TACACCACGG TATCATCCCA AAAGCCAACC ATTTGTGGAC CACGTGTTTA CTTTCACCAT TTTGGATAAT AGGATATGGT TTCGGAACTT TCAGATCATA GAAGAAGATG CTGCTCTTGT AGAAATAGGA CCTCGTTTTG TCTTAAATCT CATAAAGATT TTCCAGGGAA GTTTTGGAGG ACCAACTTTA TATGAAAATC CTCACTACCA GTCACCAAAC ATGCATCGGC GTGTCATAAG ATCCATCACA GCTGCAAAAT ACAGAGAGAA ACAGCAAGTG AAAGATGTGC AAAAACTGAG AAAGAAAGAG CCGAAGACTC TTCTTCCACA TGATCCCACT GCAGATGTTT TTGTAACACC AGCTGAGGAG AAACCAATAG AAATACAGTG GGTAAAACCA GAGCCAAAAG TTGATTTGAA AGCAAGAAAG AAACGGATTT ACAAAAGGCA AAGAAAAATG AAACAGAGGA TGGACAGTGG GAAAACAAAA TAAAGCCAAC TTTCTTGTAC AAAGTGGTTC GATAACGAAT TCTACCGGGT AGGGGAGGCG CTTTTCCCAA GGCAGTCTGG AGCATGCGCT TTAGCAGCCC CGCTGGGCAC TTGGCGCTAC ACAAGTGGCC TCTGGCCTCG CACACATTCC ACATCCACCG GTAGGCGCCA ACCGGCTCCG TTCTTTGGTG GCCCCTTCGC GCCACCTTCT ACTCCTCCCC TAGTCAGGAA GTTCCCCCCC GCCCCGCAGC TCGCGTCGTG CAGGACGTGA CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GAAGGGGTGG GTCCGGGGGC GGGCTCAGGG GCGGGCTCAG GGGCGGGGCG GGCGCCCGAA GGTCCTCCGG AGGCCCGGCA TTCTGCACGC TTCAAAAGCG CACGTCTGCC GCGCTGTTCT CCTCTTCCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC CGCCCCACGA CCCGCAGCGC CCGACCGAAA GGAGCGCACG ACCCCATGCA TCGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATAGACTGCG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT GGGTAACAGT TTCTTGAAGT TGGAGAACAA CATTCTGAGG GTAGGAGTCG AATATTAAGT AATCCTGACT CAATTAGCCA CTGTTTTGAA TCCACATACT CCAATACTCC TGAAATAGTT CATTATGGAC AGCGCAGAAA GAGCTGGGGA GAATTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GCGATTAGTC CAATTTGTTA AAGACAGGAT ATCAGTGGTC CAGGCTCTAG TTTTGACTCA ACAATATCAC CAGCTGAAGC CTATAGAGTA CGAGCCATAG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG G
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
