NM_001457.3;ABP-278; ABP-280; AOI; FH1; FLN-B; FLN1L;LRS1; SCT; TABP; TAP
100μg liquid plasmid(Instantly):$178
| name | pEnCMV-EGFP-Linker-FLNB(human)-SV40-Neo | another name | apEnCMV-EGFP-Linker-FLNB(human)-SV40-Neo |
| Insert Size (bp) | 7809bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGGGAGG AGGGGGAAGC GGTACCATGC CGGTAACCGA GAAGGATCTA GCTGAGGACG CGCCTTGGAA GAAGATCCAG CAGAACACGT TCACACGCTG GTGCAACGAG CACCTCAAGT GCGTGAACAA ACGCATCGGC AACCTGCAGA CCGACCTGAG CGACGGGCTG CGGCTCATCG CGCTGCTCGA GGTGCTCAGC CAGAAGCGCA TGTACCGCAA GTACCATCAG CGGCCCACCT TTCGCCAGAT GCAGCTCGAG AATGTGTCCG TGGCGCTCGA GTTCCTGGAC CGTGAGAGCA TCAAGCTCGT GTCCATCGAT AGCAAAGCCA TTGTGGATGG GAACCTGAAG CTCATCTTGG GTCTGGTGTG GACGCTGATC CTCCACTACT CCATCTCCAT GCCCGTGTGG GAGGATGAAG GGGATGATGA TGCCAAGAAG CAGACGCCAA AGCAGAGGCT GCTGGGGTGG ATTCAGAACA AGATCCCCTA CTTGCCCATC ACCAACTTTA ACCAGAACTG GCAAGACGGC AAAGCCCTGG GAGCCCTGGT AGACAGCTGT GCTCCAGGTC TGTGCCCAGA CTGGGAATCC TGGGACCCGC AGAAGCCTGT GGATAATGCA CGAGAAGCCA TGCAGCAGGC AGATGACTGG CTGGGTGTCC CACAGGTCAT CACTCCTGAA GAAATCATTC ACCCGGATGT GGACGAGCAC TCAGTTATGA CTTACCTGTC CCAGTTCCCC AAAGCCAAGC TCAAGCCGGG GGCTCCTCTC AAACCCAAAC TCAACCCGAA GAAAGCCAGG GCCTATGGCA GAGGAATCGA GCCCACTGGA AACATGGTGA AGCAGCCAGC CAAGTTCACT GTGGACACCA TCAGCGCCGG GCAAGGAGAC GTGATGGTGT TTGTTGAGGA CCCAGAAGGG AACAAAGAGG AGGCACAAGT GACCCCTGAC AGTGACAAGA ACAAGACATA CTCTGTGGAG TATCTGCCCA AGGTCACCGG GCTACACAAA GTCACAGTCC TCTTTGCAGG ACAGCACATC TCCAAGAGCC CATTTGAAGT GAGTGTTGAC AAGGCCCAGG GAGATGCCAG TAAAGTCACT GCAAAAGGTC CAGGGTTGGA AGCTGTAGGG AACATCGCCA ATAAGCCCAC CTACTTTGAC ATCTATACGG CAGGAGCTGG TGTGGGTGAC ATTGGTGTGG AGGTGGAAGA TCCCCAGGGG AAGAACACCG TGGAGTTGCT CGTGGAAGAC AAAGGAAACC AGGTGTATCG ATGTGTGTAC AAACCCATGC AGCCTGGCCC TCACGTGGTC AAGATCTTCT TTGCTGGGGA CACTATTCCT AAGAGTCCCT TCGTTGTGCA GGTTGGGGAA GCCTGCAATC CAAATGCCTG CCGGGCCAGT GGCCGAGGCC TACAACCCAA AGGCGTCCGT ATCCGGGAGA CCACAGATTT CAAGGTTGAC ACCAAAGCTG CAGGAAGTGG GGAGCTCGGT GTAACCATGA AGGGTCCTAA GGGTCTGGAG GAGCTGGTGA AGCAGAAAGA CTTTCTGGAT GGGGTCTACG CATTCGAGTA TTACCCCAGC ACCCCGGGGA GATACAGCAT TGCCATCACA TGGGGGGGAC ACCACATTCC AAAGAGCCCC TTTGAAGTTC AAGTTGGCCC TGAAGCGGGT ATGCAGAAAG TCCGTGCTTG GGGCCCTGGG CTCCATGGTG GGATTGTCGG GCGGTCAGCG GACTTCGTGG TAGAATCCAT TGGCTCTGAA GTGGGGTCTC TGGGGTTTGC CATTGAAGGC CCCTCTCAGG CAAAGATTGA GTACAACGAC CAGAATGATG GATCGTGTGA TGTCAAATAC TGGCCCAAGG AGCCTGGCGA ATATGCTGTT CACATCATGT GTGACGACGA AGACATCAAG GACAGCCCGT ACATGGCCTT CATCCACCCA GCCACGGGAG GCTACAACCC TGATCTGGTT CGAGCATACG GGCCAGGTTT GGAGAAATCT GGATGCATTG TCAACAACCT GGCCGAGTTC ACTGTGGATC CTAAGGATGC TGGAAAAGCT CCCTTAAAGA TATTTGCTCA GGATGGGGAA GGCCAACGCA TTGACATCCA GATGAAGAAC CGGATGGACG GCACATATGC ATGCTCATAC ACCCCGGTGA AGGCCATCAA GCACACCATT GCTGTGGTCT GGGGAGGCGT GAACATCCCG CACAGCCCCT ACAGGGTCAA CATCGGGCAA GGTAGCCATC CTCAGAAGGT CAAAGTGTTT GGGCCAGGTG TGGAGAGAAG TGGTCTGAAG GCAAATGAAC CTACACACTT CACGGTGGAC TGTACTGAGG CTGGGGAAGG TGATGTCAGT GTTGGCATTA AGTGTGATGC CCGGGTGTTA AGTGAAGATG AGGAAGACGT GGATTTTGAC ATTATTCACA ATGCCAATGA TACGTTCACA GTCAAATATG TGCCTCCTGC TGCTGGGCGA TACACTATCA AAGTTCTCTT TGCATCTCAG GAAATCCCCG CCAGCCCTTT CAGAGTCAAA GTTGACCCTT CCCACGATGC CAGCAAAGTG AAGGCAGAAG GCCCAGGGCT CAGCAAAGCA GGTGTGGAAA ATGGGAAACC GACCCACTTC ACTGTCTACA CCAAGGGGGC TGGGAAAGCC CCGCTCAACG TGCAGTTCAA CAGCCCTCTT CCTGGCGATG CAGTGAAGGA TTTGGATATC ATCGATAATT ATGACTACTC TCACACGGTT AAATATACAC CCACCCAACA GGGCAACATG CAGGTTCTGG TGACTTACGG TGGCGATCCC ATCCCTAAAA GCCCTTTCAC TGTGGGTGTT GCTGCACCGC TGGATCTGAG CAAGATAAAA CTCAATGGGC TGGAAAACAG GGTGGAAGTT GGGAAGGATC AGGAGTTCAC CGTTGATACC AGGGGGGCAG GAGGCCAGGG GAAGCTGGAC GTGACAATCC TCAGCCCCTC TCGGAAGGTC GTGCCATGCC TAGTGACACC TGTGACAGGC CGGGAGAACA GCACGGCCAA GTTCATCCCT CGGGAGGAGG GGCTGTATGC TGTAGACGTG ACCTACGATG GACACCCTGT GCCCGGGAGC CCCTACACAG TGGAGGCCTC GCTGCCACCA GATCCCAGCA AGGTGAAGGC CCACGGTCCC GGCCTCGAAG GTGGTCTCGT GGGCAAGCCT GCCGAGTTCA CCATCGATAC CAAAGGAGCT GGTACTGGAG GTCTGGGCTT AACGGTGGAA GGTCCGTGCG AGGCCAAAAT CGAGTGCTCC GACAATGGTG ATGGGACCTG CTCCGTCTCT TACCTTCCCA CAAAACCCGG GGAGTACTTC GTCAACATCC TCTTTGAAGA AGTCCACATA CCTGGGTCTC CCTTCAAAGC TGACATTGAA ATGCCCTTTG ACCCCTCTAA AGTCGTGGCA TCGGGGCCAG GTCTCGAGCA CGGGAAGGTG GGTGAAGCTG GCCTCCTTAG CGTCGACTGC TCGGAAGCGG GACCGGGGGC CCTGGGCCTG GAAGCTGTCT CGGACTCGGG AACAAAAGCC GAAGTCAGTA TTCAGAACAA CAAAGATGGC ACCTACGCGG TGACCTACGT GCCCCTGACG GCCGGCATGT ACACGTTGAC CATGAAGTAT GGTGGCGAAC TCGTGCCACA CTTCCCCGCC CGGGTCAAGG TGGAGCCCGC CGTGGACACC AGCAGGATCA AAGTCTTTGG ACCAGGAATA GAAGGGAAAG ATGTGTTCCG GGAAGCTACC ACCGACTTTA CAGTTGACTC TCGGCCGCTG ACCCAGGTTG GGGGTGACCA CATCAAGGCC CACATTGCCA ACCCCTCAGG GGCCTCCACC GAGTGCTTTG TCACAGACAA TGCGGATGGG ACCTACCAGG TGGAATACAC ACCCTTTGAG AAAGGTCTCC ATGTAGTGGA GGTGACATAT GATGACGTGC CTATCCCAAA CAGTCCCTTC AAGGTGGCTG TCACTGAAGG CTGCCAGCCA TCTAGGGTGC AAGCCCAAGG ACCTGGATTG AAAGAGGCCT TTACCAACAA GCCCAATGTC TTCACCGTGG TTACCAGAGG CGCAGGAATT GGTGGGCTTG GCATAACTGT TGAGGGACCA TCAGAGTCGA AGATAAATTG CAGAGACAAC AAGGATGGCA GCTGCAGTGC TGAGTACATT CCTTTCGCAC CGGGGGATTA CGATGTTAAT ATCACATATG GAGGAGCCCA CATCCCCGGC AGCCCCTTCA GGGTTCCTGT GAAGGATGTT GTGGACCCCA GCAAGGTCAA GATTGCCGGC CCCGGGCTGG GCTCAGGCGT CCGAGCCCGT GTCCTGCAGT CCTTCACGGT GGACAGCAGC AAGGCTGGCC TGGCTCCGCT GGAAGTGAGG GTTCTGGGCC CACGAGGCTT GGTGGAGCCA GTGAACGTGG TGGACAATGG AGATGGCACA CACACAGTAA CCTACACCCC ATCTCAGGAG GGACCTTACA TGGTCTCAGT TAAATATGCT GATGAAGAGA TTCCTCGCAG TCCCTTCAAG GTCAAGGTCC TTCCCACATA TGATGCCAGC AAAGTGACTG CCAGTGGCCC CGGCCTTAGT TCCTATGGTG TGCCTGCCAG TCTACCTGTG GACTTTGCAA TTGATGCCCG AGATGCCGGG GAAGGCCTGC TTGCTGTTCA AATAACGGAC CAAGAAGGAA AACCCAAAAG AGCCATTGTC CATGACAATA AAGATGGCAC GTATGCTGTC ACCTACATCC CCGACAAGAC TGGGCGCTAT ATGATTGGAG TCACCTACGG GGGTGACGAC ATCCCACTTT CTCCTTATCG CATCCGAGCC ACACAGACGG GTGATGCCAG CAAGTGCCTG GCCACGGGTC CTGGAATCGC CTCCACTGTG AAAACTGGCG AAGAAGTAGG CTTTGTGGTT GATGCCAAGA CTGCCGGGAA GGGTAAAGTG ACCTGCACGG TTCTGACCCC AGATGGCACT GAGGCCGAGG CCGATGTCAT TGAGAATGAA GATGGAACCT ATGACATCTT CTACACAGCT GCCAAGCCGG GCACATATGT GATCTATGTG CGCTTCGGTG GTGTTGATAT TCCTAACAGC CCCTTCACTG TCATGGCCAC AGATGGGGAA GTCACAGCCG TGGAGGAGGC ACCGGTAAAT GCATGTCCCC CTGGATTCAG GCCCTGGGTG ACCGAAGAGG CCTATGTCCC AGTGAGTGAC ATGAACGGCC TGGGATTTAA GCCTTTTGAC CTGGTCATTC CGTTTGCTGT CAGGAAAGGA GAAATCACTG GAGAGGTCCA CATGCCTTCT GGGAAGACAG CCACACCTGA GATTGTGGAC AACAAGGACG GCACGGTCAC TGTTAGATAT GCCCCCACTG AGGTCGGGCT CCATGAGATG CACATCAAAT ACATGGGCAG CCACATCCCT GAGAGCCCAC TCCAGTTCTA CGTGAACTAC CCCAACAGTG GAAGTGTTTC TGCATACGGT CCAGGCCTCG TGTATGGAGT GGCCAACAAA ACTGCCACCT TCACCATCGT CACAGAGGAT GCAGGAGAAG GTGGTCTGGA CTTGGCTATT GAGGGCCCCT CAAAAGCAGA AATCAGCTGC ATTGACAATA AAGATGGGAC ATGCACAGTG ACCTACCTGC CGACTCTGCC AGGCGACTAC AGCATTCTGG TCAAGTACAA TGACAAGCAC ATCCCTGGCA GCCCCTTCAC AGCCAAGATC ACAGATGACA GCAGGCGGTG CTCCCAGGTG AAGTTGGGCT CAGCCGCTGA CTTCCTGCTC GACATCAGTG AGACTGACCT CAGCAGCCTG ACGGCCAGCA TTAAGGCCCC ATCTGGCCGA GACGAGCCCT GTCTCCTGAA GAGGCTGCCC AACAACCACA TTGGCATCTC CTTCATCCCC CGGGAAGTGG GCGAACATCT GGTCAGCATC AAGAAAAATG GCAACCATGT GGCCAACAGC CCCGTGTCTA TCATGGTGGT CCAGTCGGAG ATTGGTGACG CCCGCCGAGC CAAAGTCTAT GGCCGCGGCC TGTCAGAAGG CCGGACTTTC GAGATGTCTG ACTTCATCGT GGACACAAGG GATGCAGGTT ATGGTGGCAT ATCCTTGGCG GTGGAAGGCC CCAGCAAAGT GGACATCCAG ACGGAGGACC TGGAAGATGG CACCTGCAAA GTCTCCTACT TCCCTACCGT GCCTGGGGTT TATATCGTCT CCACCAAATT CGCTGACGAG CACGTGCCTG GGAGCCCATT TACCGTGAAG ATCAGTGGGG AGGGAAGAGT CAAAGAGAGC ATCACCCGCA CCAGTCGGGC CCCGTCCGTG GCCACTGTCG GGAGCATTTG TGACCTGAAC CTGAAAATCC CAGAAATCAA CAGCAGTGAT ATGTCGGCCC ACGTCACCAG CCCCTCTGGC CGTGTGACTG AGGCAGAGAT TGTGCCCATG GGGAAGAACT CACACTGCGT CCGGTTTGTG CCCCAGGAGA TGGGCGTGCA CACGGTCAGC GTCAAGTACC GTGGGCAGCA CGTCACCGGC AGCCCCTTCC AGTTCACCGT GGGGCCACTT GGTGAAGGAG GCGCCCACAA GGTGCGGGCA GGAGGCCCTG GCCTGGAGAG AGGAGAAGCG GGAGTCCCAG CTGAGTTCAG CATTTGGACC CGGGAAGCAG GCGCTGGAGG CCTCTCCATC GCTGTTGAGG GCCCCAGTAA GGCCGAGATT ACATTCGATG ACCATAAAAA TGGGTCGTGC GGTGTATCTT ATATTGCCCA AGAGCCTGGT AACTACGAGG TGTCCATCAA GTTCAATGAT GAGCACATCC CGGAAAGCCC CTACCTGGTG CCGGTCATCG CACCCTCCGA CGACGCCCGC CGCCTCACTG TTATGAGCCT TCAGGAATCG GGATTAAAAG TTAACCAGCC AGCATCCTTT GCTATAAGGT TGAATGGCGC AAAAGGCAAG ATTGATGCAA AGGTGCACAG CCCCTCTGGA GCCGTGGAGG AGTGCCACGT GTCTGAGCTG GAGCCAGATA AGTATGCTGT TCGCTTCATC CCTCATGAGA ATGGTGTCCA CACCATCGAT GTCAAGTTCA ATGGGAGCCA CGTGGTTGGA AGCCCCTTCA AAGTGCGCGT TGGGGAGCCT GGACAAGCGG GGAACCCTGC CCTGGTGTCC GCCTATGGCA CGGGACTCGA AGGGGGCACC ACAGGTATCC AGTCGGAATT CTTTATTAAC ACCACCCGAG CAGGTCCAGG GACATTATCC GTCACCATCG AAGGCCCATC CAAGGTTAAA ATGGATTGCC AGGAAACACC TGAAGGGTAC AAAGTCATGT ACACCCCCAT GGCTCCTGGT AACTACCTGA TCAGCGTCAA ATACGGTGGG CCCAACCACA TCGTGGGCAG TCCCTTCAAG GCCAAGGTGA CAGGCCAGCG TCTAGTTAGC CCTGGCTCAG CCAACGAGAC CTCATCCATC CTGGTGGAGT CAGTGACCAG GTCGTCTACA GAGACCTGCT ATAGCGCCAT TCCCAAGGCA TCCTCGGACG CCAGCAAGGT GACCTCTAAG GGGGCAGGGC TCTCAAAGGC CTTTGTGGGC CAGAAGAGTT CCTTCCTGGT GGACTGCAGC AAAGCTGGCT CCAACATGCT GCTGATCGGG GTCCATGGGC CCACCACCCC CTGCGAGGAG GTCTCCATGA AGCATGTAGG CAACCAGCAA TACAACGTCA CATACGTCGT CAAGGAGAGG GGCGATTATG TGCTGGCTGT GAAGTGGGGG GAGGAACACA TCCCTGGCAG CCCTTTTCAT GTCACAGTGC CTTAACTCGA GTCTAGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG
