NM_213621.4;5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3
100μg liquid plasmid(Instantly):$105
| name | PiggyBac-CMV-HTR3A(human)(1synonymous mutations)-1-EF1a-Puro | another name | aPiggyBac-CMV-HTR3A(human)(1synonymous mutations)-1-EF1a-Puro |
| Insert Size (bp) | 1746bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | p30734 | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3′ sequencing primer | EF1a-R：gagccagtacacgacatcac | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCCAC CATGCTGCTG TGGGTCCAGC AGGCGCTGCT CGCCTTGCTC CTCCCCACAC TCCTGGCACA GGGAGAAGCC AGGAGGAGCC GAAACACCAC CAGGCCCGCT CTGCTGAGGC TGTCGGATTA CCTTTTGACC AACTACAGGA AGGGTGTGCG CCCCGTGAGG GACTGGAGGA AGCCAACCAC CGTATCCATT GACGTCATTG TCTATGCCAT CCTCAACGTG GATGAGAAGA ATCAGGTGCT GACCACCTAC ATCTGGTACC GGCAGTACTG GACTGATGAG TTTCTCCAGT GGAACCCTGA GGACTTTGAC AACATCACCA AGTTGTCCAT CCCCACGGAC AGCATCTGGG TCCCGGACAT TCTCATCAAT GAGTTCGTGG ATGTGGGGAA GTCTCCAAAT ATCCCGTACG TGTATATTCG GCATCAAGGC GAAGTTCAGA ACTACAAGCC CCTTCAGGTG GTGACTGCCT GTAGCCTCGA CATCTACAAC TTCCCCTTCG ATGTCCAGAA CTGCTCGCTG ACCTTCACCA GTTGGCTGCA CACCATCCAG GACATCAACA TCTCTTTGTG GCGCTTGCCA GAAAAGGTGA AATCCGACAG GAGTGTCTTC ATGAACCAGG GAGAGTGGGA GTTGCTGGGG GTGCTGCCCT ACTTTCGGGA GTTCAGCATG GAAAGCAGTA ACTACTATGC AGAAATGAAG TTCTATGTGG TCATCCGCCG GCGGCCCCTC TTCTATGTGG TCAGCCTGCT ACTGCCCAGC ATCTTCCTCA TGGTCATGGA CATCGTGGGC TTCTACCTGC CCCCCAACAG TGGCGAGAGG GTCTCTTTCA AGATTACACT CCTCCTGGGC TACTCGGTCT TCCTGATCAT CGTTTCTGAC ACGCTGCCGG CCACTGCCAT CGGCACTCCT CTCATTGGTA AGGCCCCTCC TGGCAGCAGA GCTCAGTCTG GTGAGAAACC CGCCCCCTCC CACCTCCTGC ATGTGTCTCT TGCCTCTGCC CTGGGCTGCA CAGGTGTCTA CTTTGTGGTG TGCATGGCTC TGCTGGTGAT AAGTTTGGCC GAGACCATCT TCATTGTGCG GCTGGTGCAC AAGCAAGACC TGCAGCAGCC CGTGCCTGCT TGGCTGCGTC ACCTGGTTCT GGAGAGAATC GCCTGGCTAC TTTGCCTGAG GGAGCAGTCA ACTTCCCAGA GGCCCCCAGC CACCTCCCAA GCCACCAAGA CTGATGACTG CTCAGCCATG GGAAACCACT GCAGCCACAT GGGAGGACCC CAGGACTTCG AGAAGAGCCC GAGGGACAGA TGTAGCCCTC CCCCACCACC TCGGGAGGCC TCGCTGGCGG TGTGTGGGCT GCTGCAGGAG CTGTCCTCCA TCCGGCAATT CCTGGAAAAG CGGGATGAGA TCCGAGAGGT GGCCCGAGAC TGGCTGCGCG TGGGCTCCGT GCTGGACAAG CTGCTATTCC ACATTTACCT GCTAGCGGTG CTGGCCTACA GCATCACCCT GGTTATGCTC TGGTCCATCT GGCAGTACGC TTGAGAATTC GAATTTAAAT CGGATCCGCG GCCGCGAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT ACGCTAGACG CCACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGAATTGA CTCAAATGAT GTCAATTAGT CTATCAGAAG CTATCTGGTC TCCCTTCCGG GGGACAAGAC ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCAAACTGG GTTCTTATAT CCCTTGCTCT GGTCAACCAG GTTGCAGGGT TTCCTGTCCT CACAGGAACG AAGTCCCTAA AGAAACAGTG GCAGCCAGGT TTAGCCCCGG AATTGACTGG ATTCCTTTTT TAGGGCCCAT TGGTATGGCT TTTTCCCCGT ATCCCCCCAG GTGTCTGCAG GCTCAAAGAG CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT TCCCCGTGCC CGGGCTGTCC CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA CCGGAGCGGA GCCCCGGGCG GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA TCCCTGGGGG CTTTGGGGGG GGGCTGTCCC TGATATCTAT AACAAGAAAA TATATATATA ATAAGTTATC ACGTAAGTAG AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT TAAAAGTCAC GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA AATCAGTGAC ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA GATGTCCTAA ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA GAATGCATGC GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAATCTAGC TGCATCAGGA TCATATCGTC GGGTCTTTTT TCCGGCTCAG TCATCGCCCA AGCTGGCGCT ATCTGGGCAT CGGGGAGGAA GAAGCCCGTG CCTTTTCCCG CGAGGTTGAA GCGGCATGGA AAGAGTTTGC CGAGGATGAC TGCTGCTGCA TTGACGTTGA GCGAAAACGC ACGTTTACCA TGATGATTCG GGAAGGTGTG GCCATGCACG CCTTTAACGG TGAACTGTTC GTTCAGGCCA CCTGGGATAC CAGTTCGTCG CGGCTTTTCC GGACACAGTT CCGGATGGTC AGCCCGAAGC GCATCAGCAA CCCGAACAAT ACCGGCGACA GCCGGAACTG CCGTGCCGGT GTGCAGATTA ATGACAGCGG TGCGGCGCTG GGATATTACG TCAGCGAGGA CGGGTATCCT GGCTGGATGC CGCAGAAATG GACATGGATA CCCCGTGAGT TACCCGGCGG GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC AT
