pGL4.16-EnIl12b(mouse)-Promoter-luc2CP-SV40-Hygro]
$84.00
In stock
SKU
PL57435
100μg liquid plasmid(Instantly):$105
| name | pGL4.16-EnIl12b(mouse)-Promoter-luc2CP-SV40-Hygro] | another name | apGL4.16-EnIl12b(mouse)-Promoter-luc2CP-SV40-Hygro] |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | EnIl12b | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCC AAGCCACAAT CATGATGATG TAAGAAAAAT GTCCCTCCAG TTTCACCAGT GACTCCAGCA GCCAGGAGGA TTTGCATAAA TTATGTCACA CAGAAAGAAA AAGTCTCGAG TCTTAAAATT CCCCCAGAAT GTTTTGACAC TAGTTTTCAG TGTTGCAATT GAGACTAGTC AGTTTCTACT TTGGGTTTCC ATCAGAAAGT TCTGTAGGAG TAGAGTATAT AAGCACCAGG AGCAGCCAAG GCAGCAGAAG GAACAGTGGG TGTCCAGGCA CATCAGACCA GGCAGCTCGC AGCAAAGCAA GCTTGGCAAT CCGGTACTGT TGGTAAAGCC ACCATGGAAG ATGCCAAAAA CATTAAGAAG GGCCCAGCGC CATTCTACCC ACTCGAAGAC GGGACCGCCG GCGAGCAGCT GCACAAAGCC ATGAAGCGCT ACGCCCTGGT GCCCGGCACC ATCGCCTTTA CCGACGCACA TATCGAGGTG GACATTACCT ACGCCGAGTA CTTCGAGATG AGCGTTCGGC TGGCAGAAGC TATGAAGCGC TATGGGCTGA ATACAAACCA TCGGATCGTG GTGTGCAGCG AGAATAGCTT GCAGTTCTTC ATGCCCGTGT TGGGTGCCCT GTTCATCGGT GTGGCTGTGG CCCCAGCTAA CGACATCTAC AACGAGCGCG AGCTGCTGAA CAGCATGGGC ATCAGCCAGC CCACCGTCGT ATTCGTGAGC AAGAAAGGGC TGCAAAAGAT CCTCAACGTG CAAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG ATAGCAAGAC CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG CCCTGATCAT GAACAGTAGT GGCAGTACCG GATTGCCCAA GGGCGTAGCC CTACCGCACC GCACCGCTTG TGTCCGATTC AGTCATGCCC GCGACCCCAT CTTCGGCAAC CAGATCATCC CCGACACCGC TATCCTCAGC GTGGTGCCAT TTCACCACGG CTTCGGCATG TTCACCACGC TGGGCTACTT GATCTGCGGC TTTCGGGTCG TGCTCATGTA CCGCTTCGAG GAGGAGCTAT TCTTGCGCAG CTTGCAAGAC TATAAGATTC AATCTGCCCT GCTGGTGCCC ACACTATTTA GCTTCTTCGC TAAGAGCACT CTCATCGACA AGTACGACCT AAGCAACTTG CACGAGATCG CCAGCGGCGG GGCGCCGCTC AGCAAGGAGG TAGGTGAGGC CGTGGCCAAA CGCTTCCACC TACCAGGCAT CCGCCAGGGC TACGGCCTGA CAGAAACAAC CAGCGCCATT CTGATCACCC CCGAAGGGGA CGACAAGCCT GGCGCAGTAG GCAAGGTGGT GCCCTTCTTC GAGGCTAAGG TGGTGGACTT GGACACCGGT AAGACACTGG GTGTGAACCA GCGCGGCGAG CTGTGCGTCC GTGGCCCCAT GATCATGAGC GGCTACGTTA ACAACCCCGA GGCTACAAAC GCTCTCATCG ACAAGGACGG CTGGCTGCAC AGCGGCGACA TCGCCTACTG GGACGAGGAC GAGCACTTCT TCATCGTGGA CCGGCTGAAG AGCCTGATCA AATACAAGGG CTACCAGGTA GCCCCAGCCG AACTGGAGAG CATCCTGCTG CAACACCCCA ACATCTTCGA CGCCGGGGTC GCCGGCCTGC CCGACGACGA TGCCGGCGAG CTGCCCGCCG CAGTCGTCGT GCTGGAACAC GGTAAAACCA TGACCGAGAA GGAGATCGTG GACTATGTGG CCAGCCAGGT TACAACCGCC AAGAAGCTGC GCGGTGGTGT TGTGTTCGTG GACGAGGTGC CTAAAGGACT GACCGGCAAG TTGGACGCCC GCAAGATCCG CGAGATTCTC ATTAAGGCCA AGAAGGGCGG CAAGATCGCC GTGAATTCTG CTTGCAAGAA CTGGTTCAGT AGCTTAAGCC ACTTTGTGAT CCACCTTAAC AGCCACGGCT TCCCTCCCGA GGTGGAGGAG CAGGCCGCCG GCACCCTGCC CATGAGCTGC GCCCAGGAGA GCGGCATGGA TAGACACCCT GCTGCTTGCG CCAGCGCCAG GATCAACGTC TAACTGCAGT CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCGTT TGCGTATTGG GCGCTCTTCC GCTGATCTGC GCAGCACCAT GGCCTGAAAT AACCTCTGAA AGAGGAACTT GGTTAGCTAC CTTCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCGAT TCTTCTGACA CTAGCGCCAC CATGAAGAAG CCCGAACTCA CCGCTACCAG CGTTGAAAAA TTTCTCATCG AGAAGTTCGA CAGTGTGAGC GACCTGATGC AGTTGTCGGA GGGCGAAGAG AGCCGAGCCT TCAGCTTCGA TGTCGGCGGA CGCGGCTATG TACTGCGGGT GAATAGCTGC GCTGATGGCT TCTACAAAGA CCGCTACGTG TACCGCCACT TCGCCAGCGC TGCACTACCC ATCCCCGAAG TGTTGGACAT CGGCGAGTTC AGCGAGAGCC TGACATACTG CATCAGTAGA CGCGCCCAAG GCGTTACTCT CCAAGACCTC CCCGAAACAG AGCTGCCTGC TGTGTTACAG CCTGTCGCCG AAGCTATGGA TGCTATTGCC GCCGCCGACC TCAGTCAAAC CAGCGGCTTC GGCCCATTCG GGCCCCAAGG CATCGGCCAG TACACAACCT GGCGGGATTT CATTTGCGCC ATTGCTGATC CCCATGTCTA CCACTGGCAG ACCGTGATGG ACGACACCGT GTCCGCCAGC GTAGCTCAAG CCCTGGACGA ACTGATGCTG TGGGCCGAAG ACTGTCCCGA GGTGCGCCAC CTCGTCCATG CCGACTTCGG CAGCAACAAC GTCCTGACCG ACAACGGCCG CATCACCGCC GTAATCGACT GGTCCGAAGC TATGTTCGGG GACAGTCAGT ACGAGGTGGC CAACATCTTC TTCTGGCGGC CCTGGCTGGC TTGCATGGAG CAGCAGACTC GCTACTTCGA GCGCCGGCAT CCCGAGCTGG CCGGCAGCCC TCGTCTGCGA GCCTACATGC TGCGCATCGG CCTGGATCAG CTCTACCAGA GCCTCGTGGA CGGCAACTTC GACGATGCTG CCTGGGCTCA AGGCCGCTGC GATGCCATCG TCCGCAGCGG GGCCGGCACC GTCGGTCGCA CACAAATCGC TCGCCGGAGC GCAGCCGTAT GGACCGACGG CTGCGTCGAG GTGCTGGCCG ACAGCGGCAA CCGCCGGCCC AGTACACGAC CGCGCGCTAA GGAGGTAGGT CGAGTTTAAA CTCTAGAACC GGTCATGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCGAACTA GATGCTGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGCGGC CGCAAATGCT AAACCACTGC AGTGGTTACC AGTGCTTGAT CAGTGAGGCA CCGATCTCAG CGATCTGCCT ATTTCGTTCG TCCATAGTGG CCTGACTCCC CGTCGTGTAG ATCACTACGA TTCGTGAGGG CTTACCATCA GGCCCCAGCG CAGCAATGAT GCCGCGAGAG CCGCGTTCAC CGGCCCCCGA TTTGTCAGCA ATGAACCAGC CAGCAGGGAG GGCCGAGCGA AGAAGTGGTC CTGCTACTTT GTCCGCCTCC ATCCAGTCTA TGAGCTGCTG TCGTGATGCT AGAGTAAGAA GTTCGCCAGT GAGTAGTTTC CGAAGAGTTG TGGCCATTGC TACTGGCATC GTGGTATCAC GCTCGTCGTT CGGTATGGCT TCGTTCAACT CTGGTTCCCA GCGGTCAAGC CGGGTCACAT GATCACCCAT ATTATGAAGA AATGCAGTCA GCTCCTTAGG GCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCGGTGTTG TCGCTCATGG TAATGGCAGC ACTACACAAT TCTCTTACCG TCATGCCATC CGTAAGATGC TTTTCCGTGA CCGGCGAGTA CTCAACCAAG TCGTTTTGTG AGTAGTGTAT ACGGCGACCA AGCTGCTCTT GCCCGGCGTC TATACGGGAC AACACCGCGC CACATAGCAG TACTTTGAAA GTGCTCATCA TCGGGAATCG TTCTTCGGGG CGGAAAGACT CAAGGATCTT GCCGCTATTG AGATCCAGTT CGATATAGCC CACTCTTGCA CCCAGTTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CGGGGTGTGC AAAAACAGGC AAGCAAAATG CCGCAAAGAA GGGAATGAGT GCGACACGAA AATGTTGGAT GCTCATACTC GTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTACT AGTACGTCTC TCAAGGATAA GTAAGTAATA TTAAGGTACG GGAGGTATTG GACAGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA CTAACATACG CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT CTCT
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