pHSE401-AtU6-FPA(arabidopsis)-sgRNA1-35S-Cas9
$99.00
In stock
SKU
PL57400
100μg liquid plasmid(Instantly):$120
| name | pHSE401-AtU6-FPA(arabidopsis)-sgRNA1-35S-Cas9 | another name | apHSE401-AtU6-FPA(arabidopsis)-sgRNA1-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Arabidopsis | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | AtU6 | Replicon | pVS1 |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TGCTCTAGCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTCGACTTG CCTTCCGCAC AATACATCAT TTCTTCTTAG CTTTTTTTCT TCTTCTTCGT TCATACAGTT TTTTTTTGTT TATCAGCTTA CATTTTCTTG AACCGTAGCT TTCGTTTTCT TCTTTTTAAC TTTCCATTCG GAGTTTTTGT ATCTTGTTTC ATAGTTTGTC CCAGGATTAG AATGATTAGG CATCGAACCT TCAAGAATTT GATTGAATAA AACATCTTCA TTCTTAAGAT ATGAAGATAA TCTTCAAAAG GCCCCTGGGA ATCTGAAAGA AGAGAAGCAG GCCCATTTAT ATGGGAAAGA ACAATAGTAT TTCTTATATA GGCCCATTTA AGTTGAAAAC AATCTTCAAA AGTCCCACAT CGCTTAGATA AGAAAACGAA GCTGAGTTTA TATACAGCTA GAGTCGAAGT AGTGATTGCT CTTCCTCCAG GTCATCCTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT TTGCAAAATT TTCCAGATCG ATTTCTTCTT CCTCTGTTCT TCGGCGTTCA ATTTCTGGGG TTTTCTCTTC GTTTTCTGTA ACTGAAACCT AAAATTTGAC CTAAAAAAAA TCTCAAATAA TATGATTCAG TGGTTTTGTA CTTTTCAGTT AGTTGAGTTT TGCAGTTCCG ATGAGATAAA CCAATAAGCT TGCATGCCTG CAGGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATAACATGG TGGAGCACGA CACACTTGTC TACTCCAAAA ATATCAAAGA TACAGTCTCA GAAGACCAAA GGGCAATTGA GACTTTTCAA CAAAGGGTAA TATCCGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTTATT GTGAAGATAG TGGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCCATCGTTG AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACCTCGACCT CAACACAACA TATACAAAAC AAACGAATCT CAAGCAATCA AGCATTCTAC TTCTATTGCA GCAATTTAAA TCATTTCTTT TAAAGCAAAA GCAATTTTCT GAAAATTTTC ACCATTTACG AACGATACTC GAGTAATCTA GATGGATTAC AAGGACCACG ACGGGGATTA CAAGGACCAC GACATTGATT ACAAGGATGA TGATGACAAG ATGGCTCCGA AGAAGAAGAG GAAGGTTGGC ATCCACGGGG TGCCAGCTGC TGACAAGAAG TACTCGATCG GCCTCGATAT TGGGACTAAC TCTGTTGGCT GGGCCGTGAT CACCGACGAG TACAAGGTGC CCTCAAAGAA GTTCAAGGTC CTGGGCAACA CCGATCGGCA TTCCATCAAG AAGAATCTCA TTGGCGCTCT CCTGTTCGAC AGCGGCGAGA CGGCTGAGGC TACGCGGCTC AAGCGCACCG CCCGCAGGCG GTACACGCGC AGGAAGAATC GCATCTGCTA CCTGCAGGAG ATTTTCTCCA ACGAGATGGC GAAGGTTGAC GATTCTTTCT TCCACAGGCT GGAGGAGTCA TTCCTCGTGG AGGAGGATAA GAAGCACGAG CGGCATCCAA TCTTCGGCAA CATTGTCGAC GAGGTTGCCT ACCACGAGAA GTACCCTACG ATCTACCATC TGCGGAAGAA GCTCGTGGAC TCCACAGATA AGGCGGACCT CCGCCTGATC TACCTCGCTC TGGCCCACAT GATTAAGTTC AGGGGCCATT TCCTGATCGA GGGGGATCTC AACCCGGACA ATAGCGATGT TGACAAGCTG TTCATCCAGC TCGTGCAGAC GTACAACCAG CTCTTCGAGG AGAACCCCAT TAATGCGTCA GGCGTCGACG CGAAGGCTAT CCTGTCCGCT AGGCTCTCGA AGTCTCGGCG CCTCGAGAAC CTGATCGCCC AGCTGCCGGG CGAGAAGAAG AACGGCCTGT TCGGGAATCT CATTGCGCTC AGCCTGGGGC TCACGCCCAA CTTCAAGTCG AATTTCGATC TCGCTGAGGA CGCCAAGCTG CAGCTCTCCA AGGACACATA CGACGATGAC CTGGATAACC TCCTGGCCCA GATCGGCGAT CAGTACGCGG ACCTGTTCCT CGCTGCCAAG AATCTGTCGG ACGCCATCCT CCTGTCTGAT ATTCTCAGGG TGAACACCGA GATTACGAAG GCTCCGCTCT CAGCCTCCAT GATCAAGCGC TACGACGAGC ACCATCAGGA TCTGACCCTC CTGAAGGCGC TGGTCAGGCA GCAGCTCCCC GAGAAGTACA AGGAGATCTT CTTCGATCAG TCGAAGAACG GCTACGCTGG GTACATTGAC GGCGGGGCCT CTCAGGAGGA GTTCTACAAG TTCATCAAGC CGATTCTGGA GAAGATGGAC GGCACGGAGG AGCTGCTGGT GAAGCTCAAT CGCGAGGACC TCCTGAGGAA GCAGCGGACA TTCGATAACG GCAGCATCCC ACACCAGATT CATCTCGGGG AGCTGCACGC TATCCTGAGG AGGCAGGAGG ACTTCTACCC TTTCCTCAAG GATAACCGCG AGAAGATCGA GAAGATTCTG ACTTTCAGGA TCCCGTACTA CGTCGGCCCA CTCGCTAGGG GCAACTCCCG CTTCGCTTGG ATGACCCGCA AGTCAGAGGA GACGATCACG CCGTGGAACT TCGAGGAGGT GGTCGACAAG GGCGCTAGCG CTCAGTCGTT CATCGAGAGG ATGACGAATT TCGACAAGAA CCTGCCAAAT GAGAAGGTGC TCCCTAAGCA CTCGCTCCTG TACGAGTACT TCACAGTCTA CAACGAGCTG ACTAAGGTGA AGTATGTGAC CGAGGGCATG AGGAAGCCGG CTTTCCTGTC TGGGGAGCAG AAGAAGGCCA TCGTGGACCT CCTGTTCAAG ACCAACCGGA AGGTCACGGT TAAGCAGCTC AAGGAGGACT ACTTCAAGAA GATTGAGTGC TTCGATTCGG TCGAGATCTC TGGCGTTGAG GACCGCTTCA ACGCCTCCCT GGGGACCTAC CACGATCTCC TGAAGATCAT TAAGGATAAG GACTTCCTGG ACAACGAGGA GAATGAGGAT ATCCTCGAGG ACATTGTGCT GACACTCACT CTGTTCGAGG ACCGGGAGAT GATCGAGGAG CGCCTGAAGA CTTACGCCCA TCTCTTCGAT GACAAGGTCA TGAAGCAGCT CAAGAGGAGG AGGTACACCG GCTGGGGGAG GCTGAGCAGG AAGCTCATCA ACGGCATTCG GGACAAGCAG TCCGGGAAGA CGATCCTCGA CTTCCTGAAG AGCGATGGCT TCGCGAACCG CAATTTCATG CAGCTGATTC ACGATGACAG CCTCACATTC AAGGAGGATA TCCAGAAGGC TCAGGTGAGC GGCCAGGGGG ACTCGCTGCA CGAGCATATC GCGAACCTCG CTGGCTCGCC AGCTATCAAG AAGGGGATTC TGCAGACCGT GAAGGTTGTG GACGAGCTGG TGAAGGTCAT GGGCAGGCAC AAGCCTGAGA ACATCGTCAT TGAGATGGCC CGGGAGAATC AGACCACGCA GAAGGGCCAG AAGAACTCAC GCGAGAGGAT GAAGAGGATC GAGGAGGGCA TTAAGGAGCT GGGGTCCCAG ATCCTCAAGG AGCACCCGGT GGAGAACACG CAGCTGCAGA ATGAGAAGCT CTACCTGTAC TACCTCCAGA ATGGCCGCGA TATGTATGTG GACCAGGAGC TGGATATTAA CAGGCTCAGC GATTACGACG TCGATCATAT CGTTCCACAG TCATTCCTGA AGGATGACTC CATTGACAAC AAGGTCCTCA CCAGGTCGGA CAAGAACCGG GGCAAGTCTG ATAATGTTCC TTCAGAGGAG GTCGTTAAGA AGATGAAGAA CTACTGGCGC CAGCTCCTGA ATGCCAAGCT GATCACGCAG CGGAAGTTCG ATAACCTCAC AAAGGCTGAG AGGGGCGGGC TCTCTGAGCT GGACAAGGCG GGCTTCATCA AGAGGCAGCT GGTCGAGACA CGGCAGATCA CTAAGCACGT TGCGCAGATT CTCGACTCAC GGATGAACAC TAAGTACGAT GAGAATGACA AGCTGATCCG CGAGGTGAAG GTCATCACCC TGAAGTCAAA GCTCGTCTCC GACTTCAGGA AGGATTTCCA GTTCTACAAG GTTCGGGAGA TCAACAATTA CCACCATGCC CATGACGCGT ACCTGAACGC GGTGGTCGGC ACAGCTCTGA TCAAGAAGTA CCCAAAGCTC GAGAGCGAGT TCGTGTACGG GGACTACAAG GTTTACGATG TGAGGAAGAT GATCGCCAAG TCGGAGCAGG AGATTGGCAA GGCTACCGCC AAGTACTTCT TCTACTCTAA CATTATGAAT TTCTTCAAGA CAGAGATCAC TCTGGCCAAT GGCGAGATCC GGAAGCGCCC CCTCATCGAG ACGAACGGCG AGACGGGGGA GATCGTGTGG GACAAGGGCA GGGATTTCGC GACCGTCAGG AAGGTTCTCT CCATGCCACA AGTGAATATC GTCAAGAAGA CAGAGGTCCA GACTGGCGGG TTCTCTAAGG AGTCAATTCT GCCTAAGCGG AACAGCGACA AGCTCATCGC CCGCAAGAAG GACTGGGATC CGAAGAAGTA CGGCGGGTTC GACAGCCCCA CTGTGGCCTA CTCGGTCCTG GTTGTGGCGA AGGTTGAGAA GGGCAAGTCC AAGAAGCTCA AGAGCGTGAA GGAGCTGCTG GGGATCACGA TTATGGAGCG CTCCAGCTTC GAGAAGAACC CGATCGATTT CCTGGAGGCG AAGGGCTACA AGGAGGTGAA GAAGGACCTG ATCATTAAGC TCCCCAAGTA CTCACTCTTC GAGCTGGAGA ACGGCAGGAA GCGGATGCTG GCTTCCGCTG GCGAGCTGCA GAAGGGGAAC GAGCTGGCTC TGCCGTCCAA GTATGTGAAC TTCCTCTACC TGGCCTCCCA CTACGAGAAG CTCAAGGGCA GCCCCGAGGA CAACGAGCAG AAGCAGCTGT TCGTCGAGCA GCACAAGCAT TACCTCGACG AGATCATTGA GCAGATTTCC GAGTTCTCCA AGCGCGTGAT CCTGGCCGAC GCGAATCTGG ATAAGGTCCT CTCCGCGTAC AACAAGCACC GCGACAAGCC AATCAGGGAG CAGGCTGAGA ATATCATTCA TCTCTTCACC CTGACGAACC TCGGCGCCCC TGCTGCTTTC AAGTACTTCG ACACAACTAT CGATCGCAAG AGGTACACAA GCACTAAGGA GGTCCTGGAC GCGACCCTCA TCCACCAGTC GATTACCGGC CTCTACGAGA CGCGCATCGA CCTGTCTCAG CTCGGGGGCG ACAAGCGGCC AGCGGCGACG AAGAAGGCGG GGCAGGCGAA GAAGAAGAAG TGAGCTCAGA GCTTTCGTTC GTATCATCGG TTTCGACAAC GTTCGTCAAG TTCAATGCAT CAGTTTCATT GCGCACACAC CAGAATCCTA CTGAGTTTGA GTATTATGGC ATTGGGAAAA CTGTTTTTCT TGTACCATTT GTTGTGCTTG TAATTTACTG TGTTTTTTAT TCGGTTTTCG CTATCGAACT GTGAAATGGA AATGGATGGA GAAGAGTTAA TGAATGATAT GGTCCTTTTG TTCATTCTCA AATTAATATT ATTTGTTTTT TCTCTTATTT GTTGTGTGTT GAATTTGAAA TTATAAGAGA TATGCAAACA TTTTGTTTTG AGTAAAAATG TGTCAAATCG TGGCCTCTAA TGACCGAAGT TAATATGAGG AGTAAAACAC TTGTAGTTGT ACCATTATGC TTATTCACTA GGCAACAAAT ATATTTTCAG ACCTAGAAAA GCTGCAAATG TTACTGAATA CAAGTATGTC CTCTTGTGTT TTAGACATTT ATGAACTTTC CTTTATGTAA TTTTCCAGAA TCCTTGTCAG ATTCTAATCA TTGCTTTATA ATTATAGTTA TACTCATGGA TTTGTAGTTG AGTATGAAAA TATTTTTTAA TGCATTTTAT GACTTGCCAA TTGATTGACA ACGAATTCGT AATCATGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGCTAGAGCA GCTTGCCAAC ATGGTGGAGC ACGACACTCT CGTCTACTCC AAGAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCTA TTGAGACTTT TCAACAAAGG GTAATATCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCAAAAGG ACAGTAGAAA AGGAAGGTGG CACCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC GTTCAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGAACATGGT GGAGCACGAC ACTCTCGTCT ACTCCAAGAA TATCAAAGAT ACAGTCTCAG AAGACCAAAG GGCTATTGAG ACTTTTCAAC AAAGGGTAAT ATCGGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTCATCA AAAGGACAGT AGAAAAGGAA GGTGGCACCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG CTATCGTTCA AGATGCCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAC GTTCCAACCA CGTCTTCAAA GCAAGTGGAT TGATGTGATA TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGGA CACGCTGAAA TCACCAGTCT CTCTCTACAA ATCTATCTCT CTCGAGCTTT CGCAGATCCG GGGGGCAATG AGATATGAAA AAGCCTGAAC TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAG TTTAGCGAGA GCCTGACCTA TTGCATCTCC CGCCGTTCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC CGCTGTTCTA CAACCGGTCG CGGAGGCTAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCACGACTC CGGGCGTATA TGCTCCGCAT TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT CGCCCGCAGA AGCGCGGCCG TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG AAACCGACGC CCCAGCACTC GTCCGAGGGC AAAGAAATAG AGTAGATGCC GACCGGGATC TGTCGATCGA CAAGCTCGAG TTTCTCCATA ATAATGTGTG AGTAGTTCCC AGATAAGGGA ATTAGGGTTC CTATAGGGTT TCGCTCATGT GTTGAGCATA TAAGAAACCC TTAGTATGTA TTTGTATTTG TAAAATACTT CTATCAATAA AATTTCTAAT TCCTAAAACC AAAATCCAGT ACTAAAATCC AGATCCCCCG AATTAATTCG GCGTTAATTC AGTACATTAA AAACGTCCGC AATGTGTTAT TAAGTTGTCT AAGCGTCAAT TTGTTTACAC CACAATATAT CCTGCCACCA GCCAGCCAAC AGCTCCCCGA CCGGCAGCTC GGCACAAAAT CACCACTCGA TACAGGCAGC CCATCAGTCC GGGACGGCGT CAGCGGGAGA GCCGTTGTAA GGCGGCAGAC TTTGCTCATG TTACCGATGC TATTCGGAAG AACGGCAACT AAGCTGCCGG GTTTGAAACA CGGATGATCT CGCGGAGGGT AGCATGTTGA TTGTAACGAT GACAGAGCGT TGCTGCCTGT GATCACCGCG GTTTCAAAAT CGGCTCCGTC GATACTATGT TATACGCCAA CTTTGAAAAC AACTTTGAAA AAGCTGTTTT CTGGTATTTA AGGTTTTAGA ATGCAAGGAA CAGTGAATTG GAGTTCGTCT TGTTATAATT AGCTTCTTGG GGTATCTTTA AATACTGTAG AAAAGAGGAA GGAAATAATA AATGGCTAAA ATGAGAATAT CACCGGAATT GAAAAAACTG ATCGAAAAAT ACCGCTGCGT AAAAGATACG GAAGGAATGT CTCCTGCTAA GGTATATAAG CTGGTGGGAG AAAATGAAAA CCTATATTTA AAAATGACGG ACAGCCGGTA TAAAGGGACC ACCTATGATG TGGAACGGGA AAAGGACATG ATGCTATGGC TGGAAGGAAA GCTGCCTGTT CCAAAGGTCC TGCACTTTGA ACGGCATGAT GGCTGGAGCA ATCTGCTCAT GAGTGAGGCC GATGGCGTCC TTTGCTCGGA AGAGTATGAA GATGAACAAA GCCCTGAAAA GATTATCGAG CTGTATGCGG AGTGCATCAG GCTCTTTCAC TCCATCGACA TATCGGATTG TCCCTATACG AATAGCTTAG ACAGCCGCTT AGCCGAATTG GATTACTTAC TGAATAACGA TCTGGCCGAT GTGGATTGCG AAAACTGGGA AGAAGACACT CCATTTAAAG ATCCGCGCGA GCTGTATGAT TTTTTAAAGA CGGAAAAGCC CGAAGAGGAA CTTGTCTTTT CCCACGGCGA CCTGGGAGAC AGCAACATCT TTGTGAAAGA TGGCAAAGTA AGTGGCTTTA TTGATCTTGG GAGAAGCGGC AGGGCGGACA AGTGGTATGA CATTGCCTTC TGCGTCCGGT CGATCAGGGA GGATATCGGG GAAGAACAGT ATGTCGAGCT ATTTTTTGAC TTACTGGGGA TCAAGCCTGA TTGGGAGAAA ATAAAATATT ATATTTTACT GGATGAATTG TTTTAGTACC TAGAATGCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGGG TGCCTTGATG TGGGCGCCGG CGGTCGAGTG GCGACGGCGC GGCTTGTCCG CGCCCTGGTA GATTGCCTGG CCGTAGGCCA GCCATTTTTG AGCGGCCAGC GGCCGCGATA GGCCGACGCG AAGCGGCGGG GCGTAGGGAG CGCAGCGACC GAAGGGTAGG CGCTTTTTGC AGCTCTTCGG CTGTGCGCTG GCCAGACAGT TATGCACAGG CCAGGCGGGT TTTAAGAGTT TTAATAAGTT TTAAAGAGTT TTAGGCGGAA AAATCGCCTT TTTTCTCTTT TATATCAGTC ACTTACATGT GTGACCGGTT CCCAATGTAC GGCTTTGGGT TCCCAATGTA CGGGTTCCGG TTCCCAATGT ACGGCTTTGG GTTCCCAATG TACGTGCTAT CCACAGGAAA CAGACCTTTT CGACCTTTTT CCCCTGCTAG GGCAATTTGC CCTAGCATCT GCTCCGTACA TTAGGAACCG GCGGATGCTT CGCCCTCGAT CAGGTTGCGG TAGCGCATGA CTAGGATCGG GCCAGCCTGC CCCGCCTCCT CCTTCAAATC GTACTCCGGC AGGTCATTTG ACCCGATCAG CTTGCGCACG GTGAAACAGA ACTTCTTGAA CTCTCCGGCG CTGCCACTGC GTTCGTAGAT CGTCTTGAAC AACCATCTGG CTTCTGCCTT GCCTGCGGCG CGGCGTGCCA GGCGGTAGAG AAAACGGCCG ATGCCGGGAT CGATCAAAAA GTAATCGGGG TGAACCGTCA GCACGTCCGG GTTCTTGCCT TCTGTGATCT CGCGGTACAT CCAATCAGCT AGCTCGATCT CGATGTACTC CGGCCGCCCG GTTTCGCTCT TTACGATCTT GTAGCGGCTA ATCAAGGCTT CACCCTCGGA TACCGTCACC AGGCGGCCGT TCTTGGCCTT CTTCGTACGC TGCATGGCAA CGTGCGTGGT GTTTAACCGA ATGCAGGTTT CTACCAGGTC GTCTTTCTGC TTTCCGCCAT CGGCTCGCCG GCAGAACTTG AGTACGTCCG CAACGTGTGG ACGGAACACG CGGCCGGGCT TGTCTCCCTT CCCTTCCCGG TATCGGTTCA TGGATTCGGT TAGATGGGAA ACCGCCATCA GTACCAGGTC GTAATCCCAC ACACTGGCCA TGCCGGCCGG CCCTGCGGAA ACCTCTACGT GCCCGTCTGG AAGCTCGTAG CGGATCACCT CGCCAGCTCG TCGGTCACGC TTCGACAGAC GGAAAACGGC CACGTCCATG ATGCTGCGAC TATCGCGGGT GCCCACGTCA TAGAGCATCG GAACGAAAAA ATCTGGTTGC TCGTCGCCCT TGGGCGGCTT CCTAATCGAC GGCGCACCGG CTGCCGGCGG TTGCCGGGAT TCTTTGCGGA TTCGATCAGC GGCCGCTTGC CACGATTCAC CGGGGCGTGC TTCTGCCTCG ATGCGTTGCC GCTGGGCGGC CTGCGCGGCC TTCAACTTCT CCACCAGGTC ATCACCCAGC GCCGCGCCGA TTTGTACCGG GCCGGATGGT TTGCGACCGC TCACGCCGAT TCCTCGGGCT TGGGGGTTCC AGTGCCATTG CAGGGCCGGC AGGCAACCCA GCCGCTTACG CCTGGCCAAC CGCCCGTTCC TCCACACATG GGGCATTCCA CGGCGTCGGT GCCTGGTTGT TCTTGATTTT CCATGCCGCC TCCTTTAGCC GCTAAAATTC ATCTACTCAT TTATTCATTT GCTCATTTAC TCTGGTAGCT GCGCGATGTA TTCAGATAGC AGCTCGGTAA TGGTCTTGCC TTGGCGTACC GCGTACATCT TCAGCTTGGT GTGATCCTCC GCCGGCAACT GAAAGTTGAC CCGCTTCATG GCTGGCGTGT CTGCCAGGCT GGCCAACGTT GCAGCCTTGC TGCTGCGTGC GCTCGGACGG CCGGCACTTA GCGTGTTTGT GCTTTTGCTC ATTTTCTCTT TACCTCATTA ACTCAAATGA GTTTTGATTT AATTTCAGCG GCCAGCGCCT GGACCTCGCG GGCAGCGTCG CCCTCGGGTT CTGATTCAAG AACGGTTGTG CCGGCGGCGG CAGTGCCTGG GTAGCTCACG CGCTGCGTGA TACGGGACTC AAGAATGGGC AGCTCGTACC CGGCCAGCGC CTCGGCAACC TCACCGCCGA TGCGCGTGCC TTTGATCGCC CGCGACACGA CAAAGGCCGC TTGTAGCCTT CCATCCGTGA CCTCAATGCG CTGCTTAACC AGCTCCACCA GGTCGGCGGT GGCCCATATG TCGTAAGGGC TTGGCTGCAC CGGAATCAGC ACGAAGTCGG CTGCCTTGAT CGCGGACACA GCCAAGTCCG CCGCCTGGGG CGCTCCGTCG ATCACTACGA AGTCGCGCCG GCCGATGGCC TTCACGTCGC GGTCAATCGT CGGGCGGTCG ATGCCGACAA CGGTTAGCGG TTGATCTTCC CGCACGGCCG CCCAATCGCG GGCACTGCCC TGGGGATCGG AATCGACTAA CAGAACATCG GCCCCGGCGA GTTGCAGGGC GCGGGCTAGA TGGGTTGCGA TGGTCGTCTT GCCTGACCCG CCTTTCTGGT TAAGTACAGC GATAACCTTC ATGCGTTCCC CTTGCGTATT TGTTTATTTA CTCATCGCAT CATATACGCA GCGACCGCAT GACGCAAGCT GTTTTACTCA AATACACATC ACCTTTTTAG ACGGCGGCGC TCGGTTTCTT CAGCGGCCAA GCTGGCCGGC CAGGCCGCCA GCTTGGCATC AGACAAACCG GCCAGGATTT CATGCAGCCG CACGGTTGAG ACGTGCGCGG GCGGCTCGAA CACGTACCCG GCCGCGATCA TCTCCGCCTC GATCTCTTCG GTAATGAAAA ACGGTTCGTC CTGGCCGTCC TGGTGCGGTT TCATGCTTGT TCCTCTTGGC GTTCATTCTC GGCGGCCGCC AGGGCGTCGG CCTCGGTCAA TGCGTCCTCA CGGAAGGCAC CGCGCCGCCT GGCCTCGGTG GGCGTCACTT CCTCGCTGCG CTCAAGTGCG CGGTACAGGG TCGAGCGATG CACGCCAAGC AGTGCAGCCG CCTCTTTCAC GGTGCGGCCT TCCTGGTCGA TCAGCTCGCG GGCGTGCGCG ATCTGTGCCG GGGTGAGGGT AGGGCGGGGG CCAAACTTCA CGCCTCGGGC CTTGGCGGCC TCGCGCCCGC TCCGGGTGCG GTCGATGATT AGGGAACGCT CGAACTCGGC AATGCCGGCG AACACGGTCA ACACCATGCG GCCGGCCGGC GTGGTGGTGT CGGCCCACGG CTCTGCCAGG CTACGCAGGC CCGCGCCGGC CTCCTGGATG CGCTCGGCAA TGTCCAGTAG GTCGCGGGTG CTGCGGGCCA GGCGGTCTAG CCTGGTCACT GTCACAACGT CGCCAGGGCG TAGGTGGTCA AGCATCCTGG CCAGCTCCGG GCGGTCGCGC CTGGTGCCGG TGATCTTCTC GGAAAACAGC TTGGTGCAGC CGGCCGCGTG CAGTTCGGCC CGTTGGTTGG TCAAGTCCTG GTCGTCGGTG CTGACGCGGG CATAGCCCAG CAGGCCAGCG GCGGCGCTCT TGTTCATGGC GTAATGTCTC CGGTTCTAGT CGCAAGTATT CTACTTTATG CGACTAAAAC ACGCGACAAG AAAACGCCAG GAAAAGGGCA GGGCGGCAGC CTGTCGCGTA ACTTAGGACT TGTGCGACAT GTCGTTTTCA GAAGACGGCT GCACTGAACG TCAGAAGCCG ACTGCACTAT AGCAGCGGAG GGGTTGGATC AAAGTACTTT GATCCCGAGG GGAACCCTGT GGTTGGCATG CACATACAAA TGGACGAACG GATAAACCTT TTCACGCCCT TTTAAATATC CGATTATTCT AA
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