pCC1BAC
$157.00
In stock
SKU
PL57380
提取要加诱导剂
100μg liquid plasmid(Instantly):$199
| name | pCC1BAC | another name | apCC1BAC |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Artificial chromosomes | Fragment type | cDNA |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | Ori2 | |
| Competence | EPI300 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Extremely low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGGCCGCAA GGGGTTCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGA GTATTCTATA GTCTCACCTA AATAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GAACCCCTTG CGGCCGCCCG GGCCGTCGAC CAATTCTCAT GTTTGACAGC TTATCATCGA ATTTCTGCCA TTCATCCGCT TATTATCACT TATTCAGGCG TAGCAACCAG GCGTTTAAGG GCACCAATAA CTGCCTTAAA AAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAACG TCTCATTTTC GCCAAAAGTT GGCCCAGGGC TTCCCGGTAT CAACAGGGAC ACCAGGATTT ATTTATTCTG CGAAGTGATC TTCCGTCACA GGTATTTATT CGCGATAAGC TCATGGAGCG GCGTAACCGT CGCACAGGAA GGACAGAGAA AGCGCGGATC TGGGAAGTGA CGGACAGAAC GGTCAGGACC TGGATTGGGG AGGCGGTTGC CGCCGCTGCT GCTGACGGTG TGACGTTCTC TGTTCCGGTC ACACCACATA CGTTCCGCCA TTCCTATGCG ATGCACATGC TGTATGCCGG TATACCGCTG AAAGTTCTGC AAAGCCTGAT GGGACATAAG TCCATCAGTT CAACGGAAGT CTACACGAAG GTTTTTGCGC TGGATGTGGC TGCCCGGCAC CGGGTGCAGT TTGCGATGCC GGAGTCTGAT GCGGTTGCGA TGCTGAAACA ATTATCCTGA GAATAAATGC CTTGGCCTTT ATATGGAAAT GTGGAACTGA GTGGATATGC TGTTTTTGTC TGTTAAACAG AGAAGCTGGC TGTTATCCAC TGAGAAGCGA ACGAAACAGT CGGGAAAATC TCCCATTATC GTAGAGATCC GCATTATTAA TCTCAGGAGC CTGTGTAGCG TTTATAGGAA GTAGTGTTCT GTCATGATGC CTGCAAGCGG TAACGAAAAC GATTTGAATA TGCCTTCAGG AACAATAGAA ATCTTCGTGC GGTGTTACGT TGAAGTGGAG CGGATTATGT CAGCAATGGA CAGAACAACC TAATGAACAC AGAACCATGA TGTGGTCTGT CCTTTTACAG CCAGTAGTGC TCGCCGCAGT CGAGCGACAG GGCGAAGCCC TCGAGCTGGT TGCCCTCGCC GCTGGGCTGG CGGCCGTCTA TGGCCCTGCA AACGCGCCAG AAACGCCGTC GAAGCCGTGT GCGAGACACC GCGGCCGGCC GCCGGCGTTG TGGATACCTC GCGGAAAACT TGGCCCTCAC TGACAGATGA GGGGCGGACG TTGACACTTG AGGGGCCGAC TCACCCGGCG CGGCGTTGAC AGATGAGGGG CAGGCTCGAT TTCGGCCGGC GACGTGGAGC TGGCCAGCCT CGCAAATCGG CGAAAACGCC TGATTTTACG CGAGTTTCCC ACAGATGATG TGGACAAGCC TGGGGATAAG TGCCCTGCGG TATTGACACT TGAGGGGCGC GACTACTGAC AGATGAGGGG CGCGATCCTT GACACTTGAG GGGCAGAGTG CTGACAGATG AGGGGCGCAC CTATTGACAT TTGAGGGGCT GTCCACAGGC AGAAAATCCA GCATTTGCAA GGGTTTCCGC CCGTTTTTCG GCCACCGCTA ACCTGTCTTT TAACCTGCTT TTAAACCAAT ATTTATAAAC CTTGTTTTTA ACCAGGGCTG CGCCCTGTGC GCGTGACCGC GCACGCCGAA GGGGGGTGCC CCCCCTTCTC GAACCCTCCC GGTCGAGTGA GCGAGGAAGC ACCAGGGAAC AGCACTTATA TATTCTGCTT ACACACGATG CCTGAAAAAA CTTCCCTTGG GGTTATCCAC TTATCCACGG GGATATTTTT ATAATTATTT TTTTTATAGT TTTTAGATCT TCTTTTTTAG AGCGCCTTGT AGGCCTTTAT CCATGCTGGT TCTAGAGAAG GTGTTGTGAC AAATTGCCCT TTCAGTGTGA CAAATCACCC TCAAATGACA GTCCTGTCTG TGACAAATTG CCCTTAACCC TGTGACAAAT TGCCCTCAGA AGAAGCTGTT TTTTCACAAA GTTATCCCTG CTTATTGACT CTTTTTTATT TAGTGTGACA ATCTAAAAAC TTGTCACACT TCACATGGAT CTGTCATGGC GGAAACAGCG GTTATCAATC ACAAGAAACG TAAAAATAGC CCGCGAATCG TCCAGTCAAA CGACCTCACT GAGGCGGCAT ATAGTCTCTC CCGGGATCAA AAACGTATGC TGTATCTGTT CGTTGACCAG ATCAGAAAAT CTGATGGCAC CCTACAGGAA CATGACGGTA TCTGCGAGAT CCATGTTGCT AAATATGCTG AAATATTCGG ATTGACCTCT GCGGAAGCCA GTAAGGATAT ACGGCAGGCA TTGAAGAGTT TCGCGGGGAA GGAAGTGGTT TTTTATCGCC CTGAAGAGGA TGCCGGCGAT GAAAAAGGCT ATGAATCTTT TCCTTGGTTT ATCAAACGTG CGCACAGTCC ATCCAGAGGG CTTTACAGTG TACATATCAA CCCATATCTC ATTCCCTTCT TTATCGGGTT ACAGAACCGG TTTACGCAGT TTCGGCTTAG TGAAACAAAA GAAATCACCA ATCCGTATGC CATGCGTTTA TACGAATCCC TGTGTCAGTA TCGTAAGCCG GATGGCTCAG GCATCGTCTC TCTGAAAATC GACTGGATCA TAGAGCGTTA CCAGCTGCCT CAAAGTTACC AGCGTATGCC TGACTTCCGC CGCCGCTTCC TGCAGGTCTG TGTTAATGAG ATCAACAGCA GAACTCCAAT GCGCCTCTCA TACATTGAGA AAAAGAAAGG CCGCCAGACG ACTCATATCG TATTTTCCTT CCGCGATATC ACTTCCATGA CGACAGGATA GTCTGAGGGT TATCTGTCAC AGATTTGAGG GTGGTTCGTC ACATTTGTTC TGACCTACTG AGGGTAATTT GTCACAGTTT TGCTGTTTCC TTCAGCCTGC ATGGATTTTC TCATACTTTT TGAACTGTAA TTTTTAAGGA AGCCAAATTT GAGGGCAGTT TGTCACAGTT GATTTCCTTC TCTTTCCCTT CGTCATGTGA CCTGATATCG GGGGTTAGTT CGTCATCATT GATGAGGGTT GATTATCACA GTTTATTACT CTGAATTGGC TATCCGCGTG TGTACCTCTA CCTGGAGTTT TTCCCACGGT GGATATTTCT TCTTGCGCTG AGCGTAAGAG CTATCTGACA GAACAGTTCT TCTTTGCTTC CTCGCCAGTT CGCTCGCTAT GCTCGGTTAC ACGGCTGCGG CGAGCGCTAG TGATAATAAG TGACTGAGGT ATGTGCTCTT CTTATCTCCT TTTGTAGTGT TGCTCTTATT TTAAACAACT TTGCGGTTTT TTGATGACTT TGCGATTTTG TTGTTGCTTT GCAGTAAATT GCAAGATTTA ATAAAAAAAC GCAAAGCAAT GATTAAAGGA TGTTCAGAAT GAAACTCATG GAAACACTTA ACCAGTGCAT AAACGCTGGT CATGAAATGA CGAAGGCTAT CGCCATTGCA CAGTTTAATG ATGACAGCCC GGAAGCGAGG AAAATAACCC GGCGCTGGAG AATAGGTGAA GCAGCGGATT TAGTTGGGGT TTCTTCTCAG GCTATCAGAG ATGCCGAGAA AGCAGGGCGA CTACCGCACC CGGATATGGA AATTCGAGGA CGGGTTGAGC AACGTGTTGG TTATACAATT GAACAAATTA ATCATATGCG TGATGTGTTT GGTACGCGAT TGCGACGTGC TGAAGACGTA TTTCCACCGG TGATCGGGGT TGCTGCCCAT AAAGGTGGCG TTTACAAAAC CTCAGTTTCT GTTCATCTTG CTCAGGATCT GGCTCTGAAG GGGCTACGTG TTTTGCTCGT GGAAGGTAAC GACCCCCAGG GAACAGCCTC AATGTATCAC GGATGGGTAC CAGATCTTCA TATTCATGCA GAAGACACTC TCCTGCCTTT CTATCTTGGG GAAAAGGACG ATGTCACTTA TGCAATAAAG CCCACTTGCT GGCCGGGGCT TGACATTATT CCTTCCTGTC TGGCTCTGCA CCGTATTGAA ACTGAGTTAA TGGGCAAATT TGATGAAGGT AAACTGCCCA CCGATCCACA CCTGATGCTC CGACTGGCCA TTGAAACTGT TGCTCATGAC TATGATGTCA TAGTTATTGA CAGCGCGCCT AACCTGGGTA TCGGCACGAT TAATGTCGTA TGTGCTGCTG ATGTGCTGAT TGTTCCCACG CCTGCTGAGT TGTTTGACTA CACCTCCGCA CTGCAGTTTT TCGATATGCT TCGTGATCTG CTCAAGAACG TTGATCTTAA AGGGTTCGAG CCTGATGTAC GTATTTTGCT TACCAAATAC AGCAATAGTA ATGGCTCTCA GTCCCCGTGG ATGGAGGAGC AAATTCGGGA TGCCTGGGGA AGCATGGTTC TAAAAAATGT TGTACGTGAA ACGGATGAAG TTGGTAAAGG TCAGATCCGG ATGAGAACTG TTTTTGAACA GGCCATTGAT CAACGCTCTT CAACTGGTGC CTGGAGAAAT GCTCTTTCTA TTTGGGAACC TGTCTGCAAT GAAATTTTCG ATCGTCTGAT TAAACCACGC TGGGAGATTA GATAATGAAG CGTGCGCCTG TTATTCCAAA ACATACGCTC AATACTCAAC CGGTTGAAGA TACTTCGTTA TCGACACCAG CTGCCCCGAT GGTGGATTCG TTAATTGCGC GCGTAGGAGT AATGGCTCGC GGTAATGCCA TTACTTTGCC TGTATGTGGT CGGGATGTGA AGTTTACTCT TGAAGTGCTC CGGGGTGATA GTGTTGAGAA GACCTCTCGG GTATGGTCAG GTAATGAACG TGACCAGGAG CTGCTTACTG AGGACGCACT GGATGATCTC ATCCCTTCTT TTCTACTGAC TGGTCAACAG ACACCGGCGT TCGGTCGAAG AGTATCTGGT GTCATAGAAA TTGCCGATGG GAGTCGCCGT CGTAAAGCTG CTGCACTTAC CGAAAGTGAT TATCGTGTTC TGGTTGGCGA GCTGGATGAT GAGCAGATGG CTGCATTATC CAGATTGGGT AACGATTATC GCCCAACAAG TGCTTATGAA CGTGGTCAGC GTTATGCAAG CCGATTGCAG AATGAATTTG CTGGAAATAT TTCTGCGCTG GCTGATGCGG AAAATATTTC ACGTAAGATT ATTACCCGCT GTATCAACAC CGCCAAATTG CCTAAATCAG TTGTTGCTCT TTTTTCTCAC CCCGGTGAAC TATCTGCCCG GTCAGGTGAT GCACTTCAAA AAGCCTTTAC AGATAAAGAG GAATTACTTA AGCAGCAGGC ATCTAACCTT CATGAGCAGA AAAAAGCTGG GGTGATATTT GAAGCTGAAG AAGTTATCAC TCTTTTAACT TCTGTGCTTA AAACGTCATC TGCATCAAGA ACTAGTTTAA GCTCACGACA TCAGTTTGCT CCTGGAGCGA CAGTATTGTA TAAGGGCGAT AAAATGGTGC TTAACCTGGA CAGGTCTCGT GTTCCAACTG AGTGTATAGA GAAAATTGAG GCCATTCTTA AGGAACTTGA AAAGCCAGCA CCCTGATGCG ACCACGTTTT AGTCTACGTT TATCTGTCTT TACTTAATGT CCTTTGTTAC AGGCCAGAAA GCATAACTGG CCTGAATATT CTCTCTGGGC CCACTGTTCC ACTTGTATCG TCGGTCTGAT AATCAGACTG GGACCACGGT CCCACTCGTA TCGTCGGTCT GATTATTAGT CTGGGACCAC GGTCCCACTC GTATCGTCGG TCTGATTATT AGTCTGGGAC CACGGTCCCA CTCGTATCGT CGGTCTGATA ATCAGACTGG GACCACGGTC CCACTCGTAT CGTCGGTCTG ATTATTAGTC TGGGACCATG GTCCCACTCG TATCGTCGGT CTGATTATTA GTCTGGGACC ACGGTCCCAC TCGTATCGTC GGTCTGATTA TTAGTCTGGA ACCACGGTCC CACTCGTATC GTCGGTCTGA TTATTAGTCT GGGACCACGG TCCCACTCGT ATCGTCGGTC TGATTATTAG TCTGGGACCA CGATCCCACT CGTGTTGTCG GTCTGATTAT CGGTCTGGGA CCACGGTCCC ACTTGTATTG TCGATCAGAC TATCAGCGTG AGACTACGAT TCCATCAATG CCTGTCAAGG GCAAGTATTG ACATGTCGTC GTAACCTGTA GAACGGAGTA ACCTCGGTGT GCGGTTGTAT GCCTGCTGTG GATTGCTGCT GTGTCCTGCT TATCCACAAC ATTTTGCGCA CGGTTATGTG GACAAAATAC CTGGTTACCC AGGCCGTGCC GGCACGTTAA CCGGGCTGCA TCCGATGCAA GTGTGTCGCT GTCGACGAGC TCGCGAGCTC GGACATGAGG TTGCCCCGTA TTCAGTGTCG CTGATTTGTA TTGTCTGAAG TTGTTTTTAC GTTAAGTTGA TGCAGATCAA TTAATACGAT ACCTGCGTCA TAATTGATTA TTTGACGTGG TTTGATGGCC TCCACGCACG TTGTGATATG TAGATGATAA TCATTATCAC TTTACGGGTC CTTTCCGGTG ATCCGACAGG TTACGGGGCG GCGACCTCGC GGGTTTTCGC TATTTATGAA AATTTTCCGG TTTAAGGCGT TTCCGTTCTT CTTCGTCATA ACTTAATGTT TTTATTTAAA ATACCCTCTG AAAAGAAAGG AAACGACAGG TGCTGAAAGC GAGCTTTTTG GCCTCTGTCG TTTCCTTTCT CTGTTTTTGT CCGTGGAATG AACAATGGAA GTCCGAGCTC ATCGCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TATTCGAT
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