NM_000245.4;"AUTS9; c-Met; DFNB97; HGFR; RCCP2
100μg liquid plasmid(Instantly):$163
| name | pEnCMV-MET(human)-Linker-mNeonGreen-SV40-Neo | another name | apEnCMV-MET(human)-Linker-mNeonGreen-SV40-Neo |
| Insert Size (bp) | 4173bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCCACC ATGAAGGCCC CCGCTGTGCT TGCACCTGGC ATCCTCGTGC TCCTGTTTAC CTTGGTGCAG AGGAGCAATG GGGAGTGTAA AGAGGCACTA GCAAAGTCCG AGATGAATGT GAATATGAAG TATCAGCTTC CCAACTTCAC CGCGGAAACA CCCATCCAGA ATGTCATTCT ACATGAGCAT CACATTTTCC TTGGTGCCAC TAACTACATT TATGTTTTAA ATGAGGAAGA CCTTCAGAAG GTTGCTGAGT ACAAGACTGG GCCTGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC TGCAGCAGCA AAGCCAATTT ATCAGGAGGT GTTTGGAAAG ATAACATCAA CATGGCTCTA GTTGTCGACA CCTACTATGA TGATCAACTC ATTAGCTGTG GCAGCGTCAA CAGAGGGACC TGCCAGCGAC ATGTCTTTCC CCACAATCAT ACTGCTGACA TACAGTCGGA GGTTCACTGC ATATTCTCCC CACAGATAGA AGAGCCCAGC CAGTGTCCTG ACTGTGTGGT GAGCGCCCTG GGAGCCAAAG TCCTTTCATC TGTAAAGGAC CGGTTCATCA ACTTCTTTGT AGGCAATACC ATAAATTCTT CTTATTTCCC AGATCATCCA TTGCATTCGA TATCAGTGAG AAGGCTAAAG GAAACGAAAG ATGGTTTTAT GTTTTTGACG GACCAGTCCT ACATTGATGT TTTACCTGAG TTCAGAGATT CTTACCCCAT TAAGTATGTC CATGCCTTTG AAAGCAACAA TTTTATTTAC TTCTTGACGG TCCAAAGGGA AACTCTAGAT GCTCAGACTT TTCACACAAG AATAATCAGG TTCTGTTCCA TAAACTCTGG ATTGCATTCC TACATGGAAA TGCCTCTGGA GTGTATTCTC ACAGAAAAGA GAAAAAAGAG ATCCACAAAG AAGGAAGTGT TTAATATACT TCAGGCTGCG TATGTCAGCA AGCCTGGGGC CCAGCTTGCT AGACAAATAG GAGCCAGCCT GAATGATGAC ATTCTTTTCG GGGTGTTCGC ACAAAGCAAG CCAGATTCTG CCGAACCAAT GGATCGATCT GCCATGTGTG CATTCCCTAT CAAATATGTC AACGACTTCT TCAACAAGAT CGTCAACAAA AACAATGTGA GATGTCTCCA GCATTTTTAC GGACCCAATC ATGAGCACTG CTTTAATAGG ACACTTCTGA GAAATTCATC AGGCTGTGAA GCGCGCCGTG ATGAATATCG AACAGAGTTT ACCACAGCTT TGCAGCGCGT TGACTTATTC ATGGGTCAAT TCAGCGAAGT CCTCTTAACA TCTATATCCA CCTTCATTAA AGGAGACCTC ACCATAGCTA ATCTTGGGAC ATCAGAGGGT CGCTTCATGC AGGTTGTGGT TTCTCGATCA GGACCATCAA CCCCTCATGT GAATTTTCTC CTGGACTCCC ATCCAGTGTC TCCAGAAGTG ATTGTGGAGC ATACATTAAA CCAAAATGGC TACACACTGG TTATCACTGG GAAGAAGATC ACGAAGATCC CATTGAATGG CTTGGGCTGC AGACATTTCC AGTCCTGCAG TCAATGCCTC TCTGCCCCAC CCTTTGTTCA GTGTGGCTGG TGCCACGACA AATGTGTGCG ATCGGAGGAA TGCCTGAGCG GGACATGGAC TCAACAGATC TGTCTGCCTG CAATCTACAA GGTTTTCCCA AATAGTGCAC CCCTTGAAGG AGGGACAAGG CTGACCATAT GTGGCTGGGA CTTTGGATTT CGGAGGAATA ATAAATTTGA TTTAAAGAAA ACTAGAGTTC TCCTTGGAAA TGAGAGCTGC ACCTTGACTT TAAGTGAGAG CACGATGAAT ACATTGAAAT GCACAGTTGG TCCTGCCATG AATAAGCATT TCAATATGTC CATAATTATT TCAAATGGCC ACGGGACAAC ACAATACAGT ACATTCTCCT ATGTGGATCC TGTAATAACA AGTATTTCGC CGAAATACGG TCCTATGGCT GGTGGCACTT TACTTACTTT AACTGGAAAT TACCTAAACA GTGGGAATTC TAGACACATT TCAATTGGTG GAAAAACATG TACTTTAAAA AGTGTGTCAA ACAGTATTCT TGAATGTTAT ACCCCAGCCC AAACCATTTC AACTGAGTTT GCTGTTAAAT TGAAAATTGA CTTAGCCAAC CGAGAGACAA GCATCTTCAG TTACCGTGAA GATCCCATTG TCTATGAAAT TCATCCAACC AAATCTTTTA TTAGTGGTGG GAGCACAATA ACAGGTGTTG GGAAAAACCT GAATTCAGTT AGTGTCCCGA GAATGGTCAT AAATGTGCAT GAAGCAGGAA GGAACTTTAC AGTGGCATGT CAACATCGCT CTAATTCAGA GATAATCTGT TGTACCACTC CTTCCCTGCA ACAGCTGAAT CTGCAACTCC CCCTGAAAAC CAAAGCCTTT TTCATGTTAG ATGGGATCCT TTCCAAATAC TTTGATCTCA TTTATGTACA TAATCCTGTG TTTAAGCCTT TTGAAAAGCC AGTGATGATC TCAATGGGCA ATGAAAATGT ACTGGAAATT AAGGGAAATG ATATTGACCC TGAAGCAGTT AAAGGTGAAG TGTTAAAAGT TGGAAATAAG AGCTGTGAGA ATATACACTT ACATTCTGAA GCCGTTTTAT GCACGGTCCC CAATGACCTG CTGAAATTGA ACAGCGAGCT AAATATAGAG TGGAAGCAAG CAATTTCTTC AACCGTCCTT GGAAAAGTAA TAGTTCAACC AGATCAGAAT TTCACAGGAT TGATTGCTGG TGTTGTCTCA ATATCAACAG CACTGTTATT ACTACTTGGG TTTTTCCTGT GGCTGAAAAA GAGAAAGCAA ATTAAAGATC TGGGCAGTGA ATTAGTTCGC TACGATGCAA GAGTACACAC TCCTCATTTG GATAGGCTTG TAAGTGCCCG AAGTGTAAGC CCAACTACAG AAATGGTTTC AAATGAATCT GTAGACTACC GAGCTACTTT TCCAGAAGAT CAGTTTCCTA ATTCATCTCA GAACGGTTCA TGCCGACAAG TGCAGTATCC TCTGACAGAC ATGTCCCCCA TCCTAACTAG TGGGGACTCT GATATATCCA GTCCATTACT GCAAAATACT GTCCACATTG ACCTCAGTGC TCTAAATCCA GAGCTGGTCC AGGCAGTGCA GCATGTAGTG ATTGGGCCCA GTAGCCTGAT TGTGCATTTC AATGAAGTCA TAGGAAGAGG GCATTTTGGT TGTGTATATC ATGGGACTTT GTTGGACAAT GATGGCAAGA AAATTCACTG TGCTGTGAAA TCCTTGAACA GAATCACTGA CATAGGAGAA GTTTCCCAAT TTCTGACCGA GGGAATCATC ATGAAAGATT TTAGTCATCC CAATGTCCTC TCGCTCCTGG GAATCTGCCT GCGAAGTGAA GGGTCTCCGC TGGTGGTCCT ACCATACATG AAACATGGAG ATCTTCGAAA TTTCATTCGA AATGAGACTC ATAATCCAAC TGTAAAAGAT CTTATTGGCT TTGGTCTTCA AGTAGCCAAA GGCATGAAAT ATCTTGCAAG CAAAAAGTTT GTCCACAGAG ACTTGGCTGC AAGAAACTGT ATGCTGGATG AAAAATTCAC AGTCAAGGTT GCTGATTTTG GTCTTGCCAG AGACATGTAT GATAAAGAAT ACTATAGTGT ACACAACAAA ACAGGTGCAA AGCTGCCAGT GAAGTGGATG GCTTTGGAAA GTCTGCAAAC TCAAAAGTTT ACCACCAAGT CAGATGTGTG GTCCTTTGGC GTGCTCCTCT GGGAGCTGAT GACAAGAGGA GCCCCACCTT ATCCTGACGT AAACACCTTT GATATAACTG TTTACTTGTT GCAAGGGAGA AGACTCCTAC AACCCGAATA CTGCCCAGAC CCCTTATATG AAGTAATGCT AAAATGCTGG CACCCTAAAG CCGAAATGCG CCCATCCTTT TCTGAACTGG TGTCCCGGAT ATCAGCGATC TTCTCTACTT TCATTGGGGA GCACTATGTC CATGTGAACG CTACTTATGT GAACGTAAAA TGTGTCGCTC CGTATCCTTC TCTGTTGTCA TCAGAAGATA ACGCTGATGA TGAGGTGGAC ACACGACCAG CCTCCTTCTG GGAGACATCA GGAGGAGGGG GAAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCTC TCTCCCAGCG ACACATGAGT TACACATCTT TGGCTCCATC AACGGTGTGG ACTTTGACAT GGTGGGTCAG GGCACCGGCA ATCCAAATGA TGGTTATGAG GAGTTAAACC TGAAGTCCAC CAAGGGTGAC CTCCAGTTCT CCCCCTGGAT TCTGGTCCCT CATATCGGGT ATGGCTTCCA TCAGTACCTG CCCTACCCTG ACGGGATGTC GCCTTTCCAG GCCGCCATGG TAGATGGCTC CGGATACCAA GTCCATCGCA CAATGCAGTT TGAAGATGGT GCCTCCCTTA CTGTTAACTA CCGCTACACC TACGAGGGAA GCCACATCAA AGGAGAGGCC CAGGTGAAGG GGACTGGTTT CCCTGCTGAC GGTCCTGTGA TGACCAACTC GCTGACCGCT GCGGACTGGT GCAGGTCGAA GAAGACTTAC CCCAACGACA AAACCATCAT CAGTACCTTT AAGTGGAGTT ACACCACTGG AAATGGCAAG CGCTACCGGA GCACTGCGCG GACCACCTAC ACCTTTGCCA AGCCAATGGC GGCTAACTAT CTGAAGAACC AGCCGATGTA CGTGTTCCGT AAGACGGAGC TCAAGCACTC CAAGACCGAG CTCAACTTCA AGGAGTGGCA AAAGGCCTTT ACCGATGTGA TGGGCATGGA CGAGCTGTAC AAGTAGGATC CACCGGATCT AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CATGCAT
