pHDE-AtU6-26-FPA(arabidopsis)-sgRNA1-NOS-mCherry-35S-Cas9
100μg liquid plasmid(Instantly):$105
100μg liquid plasmid(Instantly):$105
| name | pHDE-AtU6-26-FPA(arabidopsis)-sgRNA1-NOS-mCherry-35S-Cas9 | another name | apHDE-AtU6-26-FPA(arabidopsis)-sgRNA1-NOS-mCherry-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Arabidopsis | Prokaryotic resistance | Spe |
| Selective marker | Hyg | Fluorescent labeling | Red |
| Promoter | 35S | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTAGAGTG TGCTGGAATT GCCCTTAAGC TTCGTTGAAC AACGGAAACT CGACTTGCCT TCCGCACAAT ACATCATTTC TTCTTAGCTT TTTTTCTTCT TCTTCGTTCA TACAGTTTTT TTTTGTTTAT CAGCTTACAT TTTCTTGAAC CGTAGCTTTC GTTTTCTTCT TTTTAACTTT CCATTCGGAG TTTTTGTATC TTGTTTCATA GTTTGTCCCA GGATTAGAAT GATTAGGCAT CGAACCTTCA AGAATTTGAT TGAATAAAAC ATCTTCATTC TTAAGATATG AAGATAATCT TCAAAAGGCC CCTGGGAATC TGAAAGAAGA GAAGCAGGCC CATTTATATG GGAAAGAACA ATAGTATTTC TTATATAGGC CCATTTAAGT TGAAAACAAT CTTCAAAAGT CCCACATCGC TTAGATAAGA AAACGAAGCT GAGTTTATAT ACAGCTAGAG TCGAAGTAGT GATTGGCTCT TCCTCCAGGT CATCCTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA CCGAGTCGGT GCTTTTTTTG TCCCTTCGAA GGGCAATTCT GCAGATATCC ATCACACTGG TGAAGTGAAG CTTGGTCTCA ACTATGAGAC CTAAGCTGGC ACAACTATAT TTCCAACATC ACTAGCTACC ATCAAAAGAT TGACTTCTCA TCTTACTCGA TTGAAACCAA ATTAACATAG GGTTTTTATT TAAATAAAAG TTTAACCTTC TTTTTAAAAA ATTGTTCATA GTGTCATGTC AGAACAAGAG CTACAAATCA CACATAGCAT GCATAAGCGG AGCTATGATG AGTGGTATTG TTTTGTTCGT CACTTGTCAC TCTTTTCCAA CACATAATCC CGACAACAAC GTAAGAGCAT CTCTCTCTCT CCACACACAC TCATGCATGC ATGCATTCTT ACACGTGATT GCCATGCAAA TCTCCTTTCT CACCTATAAA TACAAACCAA CCCTTCACTA CACTCTTCAC TCAAACCAAA ACAAGAAAAC ATACACAAAT AGCAAAACGG TACCAACAAT GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGTG AGAGCTCTTG GACTCCCATG TTGGCAAAGG CAACCAAACA AACAATGAAT GATCCGCTCC TGCATATGGG GCGGTTTGAG TATTTCAACT GCCATTTGGG CTGAATTGAA GACATGCTCC TGTCAGAAAT TCCGTGATCT TACTCAATAT TCAGTAATCT CGGCCAATAT CCTAAATGTG CGTGGCTTTA TCTGTCTTTG TATTGTTTCA TCAATTCATG TAACGTTTGC TTTTCTTATG AATTTTCAAA TAAATTATCG TATTGAGACC GAATTCTGTC TAGACCTAAC TGAGTAAGCT AGCTTGTTCG AGTATTATGG CATTGGGAAA ACTGTTTTTC TTGTACCATT TGTTGTGCTT GTAATTTACT GTGTTTTTTA TTCGGTTTTC GCTATCGAAC TGTGAAATGG AAATGGATGG AGAAGAGTTA ATGAATGATA TGGTCCTTTT GTTCATTCTC AAATTAATAT TATTTGTTTT TTCTCTTATT TGTTGTGTGT TGAATTTGAA ATTATAAGAG ATATGCAAAC ATTTTGTTTT GAGTAAAAAT GTGTCAAATC GTGGCCTCTA ATGACCGAAG TTAATATGAG GAGTAAAACA CTAGATCCCC AAACAAGCTT GAAACTGAAG GCGGGAAACG ACAATCTGAT CATGAGCGGA GAATTAAGGG AGTCACGTTA TGACCCCCGC CGATGACGCG GGACAAGCCG TTTTACGTTT GGAACTGACA GAACCGCAAC GTTGAAGGAG CCACTCAGCC GCGGGTTTCT GGAGTTTAAT GAGCTAAGCA CATACGTCAG AAACCATTAT TGCGCGTTCA AAAGTCGCCT AAGGTCACTA TCAGCTAGCA AATATTTCTT GTCAAAAATG CTCCACTGAC GTTCCATAAA TTCCCCTCGG TATCCAATTA GAGTCTCATA TTCACTCTCA ATCCAAATAA TCTGCACCGG ATCCGCTAGA GGATCTCGAC CTGCAAGATC CCGGGGGGCA ATGAGATATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC GTGCTTTCAG CTTCGATATA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC TGGCAAACTG TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG ATGCTTTGGG CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA TTTCGGCTCC AACAATGTCC TGACGGACAA TGGCCGCATA ACAGCGGTCA TTGACTGGAG CGAGGCGATG TTCGGGGATT CCCAATACGA GGTCGCCAAC ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC AGACGCGCTA CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCGCGG CTCCGGGCGT ATATGCTCCG CATTGGTCTT GACCAACTCT ATCAGAGCTT GGTTGACGGC AATTTCGATG ATGCAGCTTG GGCGCAGGGT CGATGCGACG CAATCGTCCG ATCCGGAGCC GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT GTAGAAGTAC TCGCCGATAG TGGAAACCGA CGCCCCAGCA CTCGTCCGGG ATCTTGGAGG TGATGTAACA TGATCACAAG CTGATCCCCC GAATTTCCCC GATCGTTCAA ACATTTGGCA ATAAAGTTTC TTAAGATTGA ATCCTGTTGC CGGTCTTGCG ATGATTATCA TATAATTTCT GTTGAATTAC GTTAAGCATG TAATAATTAA CATGTAATGC ATGACGTTAT TTATGAGATG GGTTTTTATG ATTAGAGTCC CGCAATTATA CATTTAATAC GCGATAGAAA ACAAAATATA GCGCGCAAAC TAGGATAAAT TATCGCGCGC GGTGTCATCT ATGTTACTAG ATCGGGAATT GATCCCCCCT CGACAGCTTG CATGCCAGCT TGGGCTGCAG GTCGACGCGT CAACATGGTG GAGCACGACA CTCTCGTCTA CTCCAAGAAT ATCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGGTAATA TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCAA AAGGACAGTA GAAAAGGAAG GTGGCACCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCGTTCAA GATGCCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGATAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGATATGGA CAAGAAGTAC TCCATTGGGC TCGATATCGG CACAAACAGC GTCGGCTGGG CCGTCATTAC GGACGAGTAC AAGGTGCCGA GCAAAAAATT CAAAGTTCTG GGCAATACCG ATCGCCACAG CATAAAGAAG AACCTCATTG GCGCCCTCCT GTTCGACTCC GGGGAGACGG CCGAAGCCAC GCGGCTCAAA AGAACAGCAC GGCGCAGATA TACCCGCAGA AAGAATCGGA TCTGCTACCT GCAGGAGATC TTTAGTAATG AGATGGCTAA GGTGGATGAC TCTTTCTTCC ATAGGCTGGA GGAGTCCTTT TTGGTGGAGG AGGATAAAAA GCACGAGCGC CACCCAATCT TTGGCAATAT CGTGGACGAG GTGGCGTACC ATGAAAAGTA CCCAACCATA TATCATCTGA GGAAGAAGCT TGTAGACAGT ACTGATAAGG CTGACTTGCG GTTGATCTAT CTCGCGCTGG CGCATATGAT CAAATTTCGG GGACACTTCC TCATCGAGGG GGACCTGAAC CCAGACAACA GCGATGTCGA CAAACTCTTT ATCCAACTGG TTCAGACTTA CAATCAGCTT TTCGAAGAGA ACCCGATCAA CGCATCCGGA GTTGACGCCA AAGCAATCCT GAGCGCTAGG CTGTCCAAAT CCCGGCGGCT CGAAAACCTC ATCGCACAGC TCCCTGGGGA GAAGAAGAAC GGCCTGTTTG GTAATCTTAT CGCCCTGTCA CTCGGGCTGA CCCCCAACTT TAAATCTAAC TTCGACCTGG CCGAAGATGC CAAGCTTCAA CTGAGCAAAG ACACCTACGA TGATGATCTC GACAATCTGC TGGCCCAGAT CGGCGACCAG TACGCAGACC TTTTTTTGGC GGCAAAGAAC CTGTCAGACG CCATTCTGCT GAGTGATATT CTGCGAGTGA ACACGGAGAT CACCAAAGCT CCGCTGAGCG CTAGTATGAT CAAGCGCTAT GATGAGCACC ACCAAGACTT GACTTTGCTG AAGGCCCTTG TCAGACAGCA ACTGCCTGAG AAGTACAAGG AAATTTTCTT CGATCAGTCT AAAAATGGCT ACGCCGGATA CATTGACGGC GGAGCAAGCC AGGAGGAATT TTACAAATTT ATTAAGCCCA TCTTGGAAAA AATGGACGGC ACCGAGGAGC TGCTGGTAAA GCTTAACAGA GAAGATCTGT TGCGCAAACA GCGCACTTTC GACAATGGAA GCATCCCCCA CCAGATTCAC CTGGGCGAAC TGCACGCTAT CCTCAGGCGG CAAGAGGATT TCTACCCCTT TTTGAAAGAT AACAGGGAAA AGATTGAGAA AATCCTCACA TTTCGGATAC CCTACTATGT AGGCCCCCTC GCCCGGGGAA ATTCCAGATT CGCGTGGATG ACTCGCAAAT CAGAAGAGAC CATCACTCCC TGGAACTTCG AGGAAGTCGT GGATAAGGGG GCCTCTGCCC AGTCCTTCAT CGAAAGGATG ACTAACTTTG ATAAAAATCT GCCTAACGAA AAGGTGCTTC CTAAACACTC TCTGCTGTAC GAGTACTTCA CAGTTTATAA CGAGCTCACC AAGGTCAAAT ACGTCACAGA AGGGATGAGA AAGCCAGCAT TCCTGTCTGG AGAGCAGAAG AAAGCTATCG TGGACCTCCT CTTCAAGACG AACCGGAAAG TTACCGTGAA ACAGCTCAAA GAAGACTATT TCAAAAAGAT TGAATGTTTC GACTCTGTTG AAATCAGCGG AGTGGAGGAT CGCTTCAACG CATCCCTGGG AACGTATCAC GATCTCCTGA AAATCATTAA AGACAAGGAC TTCCTGGACA ATGAGGAGAA CGAGGACATT CTTGAGGACA TTGTCCTCAC CCTTACGTTG TTTGAAGATA GGGAGATGAT TGAAGAACGC TTGAAAACTT ACGCTCATCT CTTCGACGAC AAAGTCATGA AACAGCTCAA GAGGCGCCGA TATACAGGAT GGGGGCGGCT GTCAAGAAAA CTGATCAATG GGATCCGAGA CAAGCAGAGT GGAAAGACAA TCCTGGATTT TCTTAAGTCC GATGGATTTG CCAACCGGAA CTTCATGCAG TTGATCCATG ATGACTCTCT CACCTTTAAG GAGGACATCC AGAAAGCACA AGTTTCTGGC CAGGGGGACA GTCTTCACGA GCACATCGCT AATCTTGCAG GTAGCCCAGC TATCAAAAAG GGAATACTGC AGACCGTTAA GGTCGTGGAT GAACTCGTCA AAGTAATGGG AAGGCATAAG CCCGAGAATA TCGTTATCGA GATGGCCCGA GAGAACCAAA CTACCCAGAA GGGACAGAAG AACAGTAGGG AAAGGATGAA GAGGATTGAA GAGGGTATAA AAGAACTGGG GTCCCAAATC CTTAAGGAAC ACCCAGTTGA AAACACCCAG CTTCAGAATG AGAAGCTCTA CCTGTACTAC CTGCAGAACG GCAGGGACAT GTACGTGGAT CAGGAACTGG ACATCAATCG GCTCTCCGAC TACGACGTGG ATCATATCGT GCCCCAGTCT TTTCTCAAAG ATGATTCTAT TGATAATAAA GTGTTGACAA GATCCGATAA AAATAGAGGG AAGAGTGATA ACGTCCCCTC AGAAGAAGTT GTCAAGAAAA TGAAAAATTA TTGGCGGCAG CTGCTGAACG CCAAACTGAT CACACAACGG AAGTTCGATA ATCTGACTAA GGCTGAACGA GGTGGCCTGT CTGAGTTGGA TAAAGCCGGC TTCATCAAAA GGCAGCTTGT TGAGACACGC CAGATCACCA AGCACGTGGC CCAAATTCTC GATTCACGCA TGAACACCAA GTACGATGAA AATGACAAAC TGATTCGAGA GGTGAAAGTT ATTACTCTGA AGTCTAAGCT GGTCTCAGAT TTCAGAAAGG ACTTTCAGTT TTATAAGGTG AGAGAGATCA ACAATTACCA CCATGCGCAT GATGCCTACC TGAATGCAGT GGTAGGCACT GCACTTATCA AAAAATATCC CAAGCTTGAA TCTGAATTTG TTTACGGAGA CTATAAAGTG TACGATGTTA GGAAAATGAT CGCAAAGTCT GAGCAGGAAA TAGGCAAGGC CACCGCTAAG TACTTCTTTT ACAGCAATAT TATGAATTTT TTCAAGACCG AGATTACACT GGCCAATGGA GAGATTCGGA AGCGACCACT TATCGAAACA AACGGAGAAA CAGGAGAAAT CGTGTGGGAC AAGGGTAGGG ATTTCGCGAC AGTCCGGAAG GTCCTGTCCA TGCCGCAGGT GAACATCGTT AAAAAGACCG AAGTACAGAC CGGAGGCTTC TCCAAGGAAA GTATCCTCCC GAAAAGGAAC AGCGACAAGC TGATCGCACG CAAAAAAGAT TGGGACCCCA AGAAATACGG CGGATTCGAT TCTCCTACAG TCGCTTACAG TGTACTGGTT GTGGCCAAAG TGGAGAAAGG GAAGTCTAAA AAACTCAAAA GCGTCAAGGA ACTGCTGGGC ATCACAATCA TGGAGCGATC AAGCTTCGAA AAAAACCCCA TCGACTTTCT CGAGGCGAAA GGATATAAAG AGGTCAAAAA AGACCTCATC ATTAAGCTTC CCAAGTACTC TCTCTTTGAG CTTGAAAACG GCCGGAAACG AATGCTCGCT AGTGCGGGCG AGCTGCAGAA AGGTAACGAG CTGGCACTGC CCTCTAAATA CGTTAATTTC TTGTATCTGG CCAGCCACTA TGAAAAGCTC AAAGGGTCTC CCGAAGATAA TGAGCAGAAG CAGCTGTTCG TGGAACAACA CAAACACTAC CTTGATGAGA TCATCGAGCA AATAAGCGAA TTCTCCAAAA GAGTGATCCT CGCCGACGCT AACCTCGATA AGGTGCTTTC TGCTTACAAT AAGCACAGGG ATAAGCCCAT CAGGGAGCAG GCAGAAAACA TTATCCACTT GTTTACTCTG ACCAACTTGG GCGCGCCTGC AGCCTTCAAG TACTTCGACA CCACCATAGA CAGAAAGCGG TACACCTCTA CAAAGGAGGT CCTGGACGCC ACACTGATTC ATCAGTCAAT TACGGGGCTC TATGAAACAA GAATCGACCT CTCTCAGCTC GGTGGAGACA GCAGGGCTGA CCCCAAGAAG AAGAGGAAGG TGTGATCGAT CCAGGCCTCC CAGCTTTCGT CCGTATCATC GGTTTCGACA ACGTTCGTCA AGTTCAATGC ATCAGTTTCA TTGCCCACAC ACCAGAATCC TACTAAGTTT GAGTATTATG GCATTGGAAA AGCTGTTTTC TTCTATCATT TGTTCTGCTT GTAATTTACT GTGTTCTTTC AGTTTTTGTT TTCGGACATC AAAATGCAAA TGGATGGATA AGAGTTAATA AATGATATGG TCCTTTTGTT CATTCTCAAA TTATTATTAT CTGTTGTTTT TACTTTAATG GGTTGAATTT AAGTAAGAAA GGAACTAACA GTGTGATATT AAGGTGCAAT GTTAGACATA TAAAACAGTC TTTCACCTCT CTTTGGTTAT GTCTTGAATT GGTTTGTTTC TTCACTTATC TGTGTAATCA AGTTTACTAT GAGTCTATGA TCAAGTAATT ATGCAATCAA GTTAAGTACA GTATAGGCTT TTTGTGTCGA GGGGGTACCG AGTCGAGGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGGGTAC CGAGCTCGAA TTCAATTCGG CGTTAATTCA GTACATTAAA AACGTCCGCA ATGTGTTATT AAGTTGTCTA AGCGTCAATT TGTTTACACC ACAATATATC CTGCCACCAG CCAGCCAACA GCTCCCCGAC CGGCAGCTCG GCACAAAATC ACCACTCGAT ACAGGCAGCC CATCAGTCCG GGACGGCGTC AGCGGGAGAG CCGTTGTAAG GCGGCAGACT TTGCTCATGT TACCGATGCT ATTCGGAAGA ACGGCAACTA AGCTGCCGGG TTTGAAACAC GGATGATCTC GCGGAGGGTA GCATGTTGAT TGTAACGATG ACAGAGCGTT GCTGCCTGTG ATCAATTCGG GCACGAACCC AGTGGACATA AGCCTCGTTC GGTTCGTAAG CTGTAATGCA AGTAGCGTAA CTGCCGTCAC GCAACTGGTC CAGAACCTTG ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA TGGCTTCTTG TTATGACATG TTTTTTTGGG GTACAGTCTA TGCCTCGGGC ATCCAAGCAG CAAGCGCGTT ACGCCGTGGG TCGATGTTTG ATGTTATGGA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC AAAGTTAAAC ATCATGGGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT AGTTGGCGTC ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC CGCAGTGGAT GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA CGGTGACCGT AAGGCTTGAT GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC CCCTGGAGAG AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT CATTCCGTGG CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA CATTCTTGCA GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC AAAAGCAAGA GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC GGTTCCTGAA CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC GCCCGACTGG GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG CGCAGTAACC GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT GCCGGCCCAG TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA AGATCGCTTG GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG TGAAAGGCGA GATCACCAAG GTAGTCGGCA AATAATGTCT AGCTAGAAAT TCGTTCAAGC CGACGCCGCT TCGCCGGCGT TAACTCAAGC GATTAGATGC ACTAAGCACA TAATTGCTCA CAGCCAAACT ATCAGGTCAA GTCTGCTTTT ATTATTTTTA AGCGTGCATA ATAAGCCCTA CACAAATTGG GAGATATATC ATGCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGGGTGCC TTGATGTGGG CGCCGGCGGT CGAGTGGCGA CGGCGCGGCT TGTCCGCGCC CTGGTAGATT GCCTGGCCGT AGGCCAGCCA TTTTTGAGCG GCCAGCGGCC GCGATAGGCC GACGCGAAGC GGCGGGGCGT AGGGAGCGCA GCGACCGAAG GGTAGGCGCT TTTTGCAGCT CTTCGGCTGT GCGCTGGCCA GACAGTTATG CACAGGCCAG GCGGGTTTTA AGAGTTTTAA TAAGTTTTAA AGAGTTTTAG GCGGAAAAAT CGCCTTTTTT CTCTTTTATA TCAGTCACTT ACATGTGTGA CCGGTTCCCA ATGTACGGCT TTGGGTTCCC AATGTACGGG TTCCGGTTCC CAATGTACGG CTTTGGGTTC CCAATGTACG TGCTATCCAC AGGAAAGAGA CCTTTTCGAC CTTTTTCCCC TGCTAGGGCA ATTTGCCCTA GCATCTGCTC CGTACATTAG GAACCGGCGG ATGCTTCGCC CTCGATCAGG TTGCGGTAGC GCATGACTAG GATCGGGCCA GCCTGCCCCG CCTCCTCCTT CAAATCGTAC TCCGGCAGGT CATTTGACCC GATCAGCTTG CGCACGGTGA AACAGAACTT CTTGAACTCT CCGGCGCTGC CACTGCGTTC GTAGATCGTC TTGAACAACC ATCTGGCTTC TGCCTTGCCT GCGGCGCGGC GTGCCAGGCG GTAGAGAAAA CGGCCGATGC CGGGATCGAT CAAAAAGTAA TCGGGGTGAA CCGTCAGCAC GTCCGGGTTC TTGCCTTCTG TGATCTCGCG GTACATCCAA TCAGCTAGCT CGATCTCGAT GTACTCCGGC CGCCCGGTTT CGCTCTTTAC GATCTTGTAG CGGCTAATCA AGGCTTCACC CTCGGATACC GTCACCAGGC GGCCGTTCTT GGCCTTCTTC GTACGCTGCA TGGCAACGTG CGTGGTGTTT AACCGAATGC AGGTTTCTAC CAGGTCGTCT TTCTGCTTTC CGCCATCGGC TCGCCGGCAG AACTTGAGTA CGTCCGCAAC GTGTGGACGG AACACGCGGC CGGGCTTGTC TCCCTTCCCT TCCCGGTATC GGTTCATGGA TTCGGTTAGA TGGGAAACCG CCATCAGTAC CAGGTCGTAA TCCCACACAC TGGCCATGCC GGCCGGCCCT GCGGAAACCT CTACGTGCCC GTCTGGAAGC TCGTAGCGGA TCACCTCGCC AGCTCGTCGG TCACGCTTCG ACAGACGGAA AACGGCCACG TCCATGATGC TGCGACTATC GCGGGTGCCC ACGTCATAGA GCATCGGAAC GAAAAAATCT GGTTGCTCGT CGCCCTTGGG CGGCTTCCTA ATCGACGGCG CACCGGCTGC CGGCGGTTGC CGGGATTCTT TGCGGATTCG ATCAGCGGCC GCTTGCCACG ATTCACCGGG GCGTGCTTCT GCCTCGATGC GTTGCCGCTG GGCGGCCTGC GCGGCCTTCA ACTTCTCCAC CAGGTCATCA CCCAGCGCCG CGCCGATTTG TACCGGGCCG GATGGTTTGC GACCGTCACG CCGATTCCTC GGGCTTGGGG GTTCCAGTGC CATTGCAGGG CCGGCAGACA ACCCAGCCGC TTACGCCTGG CCAACCGCCC GTTCCTCCAC ACATGGGGCA TTCCACGGCG TCGGTGCCTG GTTGTTCTTG ATTTTCCATG CCGCCTCCTT TAGCCGCTAA AATTCATCTA CTCATTTATT CATTTGCTCA TTTACTCTGG TAGCTGCGCG ATGTATTCAG ATAGCAGCTC GGTAATGGTC TTGCCTTGGC GTACCGCGTA CATCTTCAGC TTGGTGTGAT CCTCCGCCGG CAACTGAAAG TTGACCCGCT TCATGGCTGG CGTGTCTGCC AGGCTGGCCA ACGTTGCAGC CTTGCTGCTG CGTGCGCTCG GACGGCCGGC ACTTAGCGTG TTTGTGCTTT TGCTCATTTT CTCTTTACCT CATTAACTCA AATGAGTTTT GATTTAATTT CAGCGGCCAG CGCCTGGACC TCGCGGGCAG CGTCGCCCTC GGGTTCTGAT TCAAGAACGG TTGTGCCGGC GGCGGCAGTG CCTGGGTAGC TCACGCGCTG CGTGATACGG GACTCAAGAA TGGGCAGCTC GTACCCGGCC AGCGCCTCGG CAACCTCACC GCCGATGCGC GTGCCTTTGA TCGCCCGCGA CACGACAAAG GCCGCTTGTA GCCTTCCATC CGTGACCTCA ATGCGCTGCT TAACCAGCTC CACCAGGTCG GCGGTGGCCC ATATGTCGTA AGGGCTTGGC TGCACCGGAA TCAGCACGAA GTCGGCTGCC TTGATCGCGG ACACAGCCAA GTCCGCCGCC TGGGGCGCTC CGTCGATCAC TACGAAGTCG CGCCGGCCGA TGGCCTTCAC GTCGCGGTCA ATCGTCGGGC GGTCGATGCC GACAACGGTT AGCGGTTGAT CTTCCCGCAC GGCCGCCCAA TCGCGGGCAC TGCCCTGGGG ATCGGAATCG ACTAACAGAA CATCGGCCCC GGCGAGTTGC AGGGCGCGGG CTAGATGGGT TGCGATGGTC GTCTTGCCTG ACCCGCCTTT CTGGTTAAGT ACAGCGATAA CCTTCATGCG TTCCCCTTGC GTATTTGTTT ATTTACTCAT CGCATCATAT ACGCAGCGAC CGCATGACGC AAGCTGTTTT ACTCAAATAC ACATCACCTT TTTAGACGGC GGCGCTCGGT TTCTTCAGCG GCCAAGCTGG CCGGCCAGGC CGCCAGCTTG GCATCAGACA AACCGGCCAG GATTTCATGC AGCCGCACGG TTGAGACGTG CGCGGGCGGC TCGAACACGT ACCCGGCCGC GATCATCTCC GCCTCGATCT CTTCGGTAAT GAAAAACGGT TCGTCCTGGC CGTCCTGGTG CGGTTTCATG CTTGTTCCTC TTGGCGTTCA TTCTCGGCGG CCGCCAGGGC GTCGGCCTCG GTCAATGCGT CCTCACGGAA GGCACCGCGC CGCCTGGCCT CGGTGGGCGT CACTTCCTCG CTGCGCTCAA GTGCGCGGTA CAGGGTCGAG CGATGCACGC CAAGCAGTGC AGCCGCCTCT TTCACGGTGC GGCCTTCCTG GTCGATCAGC TCGCGGGCGT GCGCGATCTG TGCCGGGGTG AGGGTAGGGC GGGGGCCAAA CTTCACGCCT CGGGCCTTGG CGGCCTCGCG CCCGCTCCGG GTGCGGTCGA TGATTAGGGA ACGCTCGAAC TCGGCAATGC CGGCGAACAC GGTCAACACC ATGCGGCCGG CCGGCGTGGT GGTGTCGGCC CACGGCTCTG CCAGGCTACG CAGGCCCGCG CCGGCCTCCT GGATGCGCTC GGCAATGTCC AGTAGGTCGC GGGTGCTGCG GGCCAGGCGG TCTAGCCTGG TCACTGTCAC AACGTCGCCA GGGCGTAGGT GGTCAAGCAT CCTGGCCAGC TCCGGGCGGT CGCGCCTGGT GCCGGTGATC TTCTCGGAAA ACAGCTTGGT GCAGCCGGCC GCGTGCAGTT CGGCCCGTTG GTTGGTCAAG TCCTGGTCGT CGGTGCTGAC GCGGGCATAG CCCAGCAGGC CAGCGGCGGC GCTCTTGTTC ATGGCGTAAT GTCTCCGGTT CTAGTCGCAA GTATTCTACT TTATGCGACT AAAACACGCG ACAAGAAAAC GCCAGGAAAA GGGCAGGGCG GCAGCCTGTC GCGTAACTTA GGACTTGTGC GACATGTCGT TTTCAGAAGA CGGCTGCACT GAACGTCAGA AGCCGACTGC ACTATAGCAG CGGAGGGGTT GGATCAAAGT ACTTTAAAGT ACTTTAAAGT ACTTTAAAGT ACTTTGATCC CGAGGGGAAC CCTGTGGTTG GCATGCACAT ACAAATGGAC GAACGGATAA ACCTTTTCAC GCCCTTTTAA ATATCCGATT ATTCTAATAA ACGCTCTTTT CTCTTAG