NM_000682.7;ADRA2L1; ADRA2RL1; ADRARL1; alpha-2BAR;ALPHA2BAR; FAME2
100μg liquid plasmid(Instantly):$105
| name | PiggyBac-CMV-ADRA2B(human)(2synonymous mutations)-EF1a-Puro | another name | aPiggyBac-CMV-ADRA2B(human)(2synonymous mutations)-EF1a-Puro |
| Insert Size (bp) | 1353bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | EF1a-R：gagccagtacacgacatcac | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGGACC ACCAGGACCC CTACTCCGTG CAGGCCACAG CGGCCATAGC GGCGGCCATC ACCTTCCTCA TTCTCTTTAC CATCTTCGGC AACGCTCTGG TCATCCTGGC TGTGTTGACC AGCCGCTCGC TGCGCGCCCC TCAGAACCTG TTCCTGGTGT CGCTGGCCGC CGCCGACATC CTGGTGGCCA CGCTCATCAT CCCTTTCTCG CTGGCCAACG AGCTGCTGGG CTACTGGTAC TTCCGGCGCA CGTGGTGCGA GGTGTACCTG GCGCTCGACG TGCTCTTCTG CACCTCGTCC ATCGTGCACC TGTGCGCCAT CAGCCTGGAC CGCTACTGGG CCGTGAGCCG CGCGCTGGAG TACAACTCCA AGCGCACCCC GCGCCGCATC AAGTGCATCA TCCTCACTGT GTGGCTCATC GCCGCCGTCA TCTCGCTGCC GCCCCTCATC TACAAGGGCG ACCAGGGCCC CCAGCCGCGC GGGCGCCCCC AGTGCAAGCT CAACCAGGAG GCCTGGTACA TCCTGGCCTC CAGCATCGGA TCTTTCTTTG CTCCTTGCCT CATCATGATC CTTGTCTACC TGCGCATCTA CCTGATCGCC AAACGCAGCA ACCGCAGAGG TCCCAGGGCC AAGGGGGGGC CTGGGCAGGG TGAGTCCAAG CAGCCCCGAC CCGACCATGG TGGGGCTTTG GCCTCAGCCA AACTGCCAGC CCTGGCCTCT GTGGCTTCTG CCAGAGAGGT CAACGGACAC TCGAAGTCCA CTGGGGAGAA GGAGGAGGGG GAGACCCCTG AAGATACTGG GACCCGGGCC TTGCCACCCA GTTGGGCTGC CCTTCCCAAC TCAGGCCAGG GCCAGAAGGA GGGTGTTTGT GGGGCATCTC CAGAGGATGA AGCTGAAGAG GAGGAAGAGG AGGAGGAGGA GGAGGAAGAG TGTGAACCCC AGGCAGTGCC AGTGTCTCCG GCCTCAGCTT GCAGCCCCCC GCTGCAGCAG CCACAGGGCT CCCGGGTGCT GGCCACCCTA CGTGGCCAGG TGCTCCTGGG CAGGGGCGTG GGTGCTATAG GTGGGCAGTG GTGGCGTCGA CGGGCGCAGC TGACCCGGGA GAAGCGCTTC ACCTTCGTGC TGGCTGTGGT CATTGGCGTT TTTGTGCTCT GCTGGTTCCC CTTCTTCTTC AGCTACAGCC TGGGAGCCAT CTGCCCGAAG CACTGCAAGG TGCCCCATGG CCTCTTCCAG TTCTTCTTCT GGATCGGCTA CTGCAACAGC TCACTGAACC CTGTTATCTA CACCATCTTC AACCAGGACT TCCGCCGTGC CTTCCGGAGG ATCCTGTGCC GCCCGTGGAC CCAGACGGCC TGGTGAGCGG CCGCGAAGGA TCTGCGATCG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CGCTAGACGC CACCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCAGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGAATTGAC TCAAATGATG TCAATTAGTC TATCAGAAGC TATCTGGTCT CCCTTCCGGG GGACAAGACA TCCCTGTTTA ATATTTAAAC AGCAGTGTTC CCAAACTGGG TTCTTATATC CCTTGCTCTG GTCAACCAGG TTGCAGGGTT TCCTGTCCTC ACAGGAACGA AGTCCCTAAA GAAACAGTGG CAGCCAGGTT TAGCCCCGGA ATTGACTGGA TTCCTTTTTT AGGGCCCATT GGTATGGCTT TTTCCCCGTA TCCCCCCAGG TGTCTGCAGG CTCAAAGAGC AGCGAGAAGC GTTCAGAGGA AAGCGATCCC GTGCCACCTT CCCCGTGCCC GGGCTGTCCC CGCACGCTGC CGGCTCGGGG ATGCGGGGGG AGCGCCGGAC CGGAGCGGAG CCCCGGGCGG CTCGCTGCTG CCCCCTAGCG GGGGAGGGAC GTAATTACAT CCCTGGGGGC TTTGGGGGGG GGCTGTCCCT GATATCTATA ACAAGAAAAT ATATATATAA TAAGTTATCA CGTAAGTAGA ACATGAAATA ACAATATAAT TATCGTATGA GTTAAATCTT AAAAGTCACG TAAAAGATAA TCATGCGTCA TTTTGACTCA CGCGGTCGTT ATAGTTCAAA ATCAGTGACA CTTACCGCAT TGACAAGCAC GCCTCACGGG AGCTCCAAGC GGCGACTGAG ATGTCCTAAA TGCACAGCGA CGGATTCGCG CTATTTAGAA AGAGAGAGCA ATATTTCAAG AATGCATGCG TCAATTTTAC GCAGACTATC TTTCTAGGGT TAATCTAGCT GCATCAGGAT CATATCGTCG GGTCTTTTTT CCGGCTCAGT CATCGCCCAA GCTGGCGCTA TCTGGGCATC GGGGAGGAAG AAGCCCGTGC CTTTTCCCGC GAGGTTGAAG CGGCATGGAA AGAGTTTGCC GAGGATGACT GCTGCTGCAT TGACGTTGAG CGAAAACGCA CGTTTACCAT GATGATTCGG GAAGGTGTGG CCATGCACGC CTTTAACGGT GAACTGTTCG TTCAGGCCAC CTGGGATACC AGTTCGTCGC GGCTTTTCCG GACACAGTTC CGGATGGTCA GCCCGAAGCG CATCAGCAAC CCGAACAATA CCGGCGACAG CCGGAACTGC CGTGCCGGTG TGCAGATTAA TGACAGCGGT GCGGCGCTGG GATATTACGT CAGCGAGGAC GGGTATCCTG GCTGGATGCC GCAGAAATGG ACATGGATAC CCCGTGAGTT ACCCGGCGGG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA T
