pDHtsk-gdpA-EGFP(156-238aa)-Linker2-GeneB-G418
$113.00
In stock
SKU
PL57135
100μg liquid plasmid(Instantly):$134
| name | pDHtsk-gdpA-EGFP(156-238aa)-Linker2-GeneB-G418 | another name | apDHtsk-gdpA-EGFP(156-238aa)-Linker2-GeneB-G418 |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACATTAACG TTTACAATTT CGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGGT ACCGCATGCG GAGAGACGGA CGGACGCAGA GAGAAGGGCT GAGTAATAAG CGCCACTGCG CCAGACAGCT CTGGCGGCTC TGAGGTGCAG TGGATGATTA TTAATCCGGG ACCGGCCGCC CCTCCGCCCC GAAGTGGAAA GGCTGGTGTG CCCCTCGTTG ACCAAGAATC TATTGCATCA TCGGAGAATA TGGAGCTTCA TCGAATCACC GGCAGTAAGC GAAGGAGAAT GTGAAGCCAG GGGTGTATAG CCGTCGGCGA AATAGCATGC CATTAACCTA GGTACAGAAG TCCAATTGCT TCCGATCTGG TAAAAGATTC ACGAGATAGT ACCTTCTCCG AAGTAGGTAG AGCGAGTACC CGGCGCGTAA GCTCCCTAAT TGGCCCATCC GGCATCTGTA GGGCGTCCAA ATATCGTGCC TCTCCTGCTT TGCCCGGTGT ATGAAACCGG AAAGGCCGCT CAGGAGCTGG CCAGCGGCGC AGACCGGGAA CACAAGCTGG CAGTCGACCC ATCCGGTGCT CTGCACTCGA CCTGCTGAGG TCCCTCAGTC CCTGGTAGGC AGCTTTGCCC CGTCTGTCCG CCCGGTGTGT CGGCGGGGTT GACAAGGTCG TTGCGTCAGT CCAACATTTG TTGCCATATT TTCCTGCTCT CCCCACCAGC TGCTCTTTTC TTTTCTCTTT CTTTTCCCAT CTTCAGTATA TTCATCTTCC CATCCAAGAA CCTTTAATCA AGCTTATCGA TACCGTCGAC CTCGACTCTA GAGCCACCAT GAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG CGCTCCATCG CCACCCACGT GATGCAAGGG TCAAGGAAGA TCTCCTTCAA CATCTCCGAA CAGTATGAAA TCGCTGACAT CATCGGAGAG GGTGCCTATG GTGTTGTCTG TTCGGCAATT CACAAGCCTT CCGGCCAGAA GGTCGCGATC AAGAAGATCA TCCCCTTCGA CCACTCGATG TTCTGTCTGC GGACCCTGCG TGAAATGAAG CTGCTGCGCT ATTTCAACCA CGAAAACATC ATCTCCATCT TGGATATCCA GAGACCCCGC AGCTATGACT CGTTCAATGA AGTATATCTC ATTCAAGAGC TGATGGAAAC CGATATGCAT CGAGTTATCC GTACCCAAGA CCTCTCCGAC GACCACTGCC AGTACTTCAT CTACCAGACC CTCCGTGCGC TCAAAGCAAT GCACTCCGCA AACGTTCTCC ACCGTGACCT GAAGCCATCC AACCTGTTAC TCAACGCAAA CTGCGACCTG AAAGTCTGCG ACTTTGGTCT GGCCCGTTCT GCCGCGTCAA CCGACGACAA CTCCGGATTC ATGACGGAAT ACGTTGCGAC TCGTTGGTAC CGTGCGCCAG AAATCATGTT GACCTTCAAG GAATACACAA AAGCTATTGA TGTGTGGAGT GTTGGATGTA TATTGGCCGA GATGCTGAGC GGAAAGCCCT TGTTCCCCGG AAAAGATTAT CACCACCAAT TGACCCTTAT CCTTGACGTC CTGGGTACAC CAACCATGGA GGACTACTAC GGAATCAAGT CCCGCCGTGC GCGCGAGTAC ATCCGCTCTC TCCCCTTCAA GAAGAAGATT CCCTTCAAGG CCCTCTTCCC CAAGAGCAAC GACCAGGCCC TTGACCTCCT GGAGAGACTT CTCGCATTCA ACCCCGCCAA GCGTATCACA GTCGAAGAAG CCCTCCAGCA CCCCTACCTG GAGCCGTACC ATGACCCGGA CGATGAGCCC ACTGCTCCCC CGATTCCGGA TGGATTCTTT GATTTCGACA AGAACAAGGA TGCGCTCAGC AAGGAGCAGC TGAAGTACTT GATTTACGAA GAGATCATGC GGTAAGGATC CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC GACGTTAACT GATATTGAAG GAGCACTTTT TGGGCTTGGC TGGAGCTAGT GGAGGTCAAC AATGAATGCC TATTTTGGTT TAGTCGTCCA GGCGGTGAGC ACAAAATTTG TGTCGTTTGA CAAGATGGTT CATTTAGGCA ACTGGTCAGA TCAGCCCCAC TTGTAGCAGT AGCGGCGGCG CTCGAAGTGT GACTCTTATT AGCAGACAGG AACTAGGACA TTATCATCAT CTGCTGCTTG GTGCACGATA ACTTGGTGCG TTTGTCAAGC AAGGTAAGTG AACGACCCGG TCATACCTTC TTAAGGGGTT TCTGGAGTTT AATGAGCTAA GCACATACGT CAGAAACCAT TATTGCGCGT TCAAAAGTCG CCTAAGGTCA CTATCAGCTA GCAAATATTT CTTGTCAAAA ATGCTCCACT GACGTTCCAT AAATTCCCCT CGGTATCCAA TTAGAGTCTC ATATTCACTC TCAATCCAAA TAATCTGCAC CGGATCTGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGATGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACGGGATC TCTGCGGAAC AGGCGGTCGA AGGTGCCGAT ATCATTACGA CAGCAACGGC CGACAAGCAC AACGCCACGA TCCTGAGCGA CAATATGATC GGGCCCGGCG TCCACATCAA CGGCGTCGGC GGCGACTGCC CAGGCAAGAC CGAGATGCAC CGCGATATCT TGCTGCGTTC GGATATTTTC GTGGAGTTCC CGCCACAGAC CCGGATGATC CCCGATCGTT CAAACATTTG GCAATAAAGT TTCTTAAGAT TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT TCTGTTGAAT TACGTTAAGC ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG ATGGGTTTTT ATGATTAGAG TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT ATAGCGCGCA AACTAGGATA AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCACGC GTTTTCGGGT TTACCTCTTC CAGATACAGC TCAACTGCAA TGCATTAATG CATTGGACCT CGCAACCCTA GTACGCCCTT CAGGCTCCGG CGAAGCAGAA GAATAGCTTA GCAGAGTCTA TTTTCATTTT CGGGAGACGA GATCAAGCAG ATCAACGGTC GTCAAGAGAC CTACGAGACT GAGGAATCCG CTCTTGGCTC CACGCGACTA TATATTTGTC TCTAATTGTA CTTTGACATG CTCCTCTTCT TTACTCTGAT AGCTTGACTA TGAAAATTCC GTCACCAGCC CTGACTAGTT CTAGAGCGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT CCGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGhCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGAAG GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG CTAGAGCAGC TTGAGCTTGG ATCAGATTGT CGTTTCCCGC CTTCAGTTTA AACTATCAGT GTTTGACAGG ATATATTGGC GGGTAAACCT AAGAGAAAAG AGCGTTTATT AGAATAACGG ATATTTAAAA GGGCGTGAAA AGGTTTATCC GTTCGTCCAT TTGTATGTGC ATGCCAACCA CAGGGTTCCC CTCGGGATCA AAGTACTTTG ATCCAACCCC TCCGCTGCTA TAGTGCAGTC GGCTTCTGAC GTTCAGTGCA GCCGTCTTCT GAAAACGACA TGTCGCACAA GTCCTAAGTT ACGCGACAGG CTGCCGCCCT GCCCTTTTCC TGGCGTTTTC TTGTCGCGTG TTTTAGTCGC ATAAAGTAGA ATACTTGCGA CTAGAACCGG AGACATTACG CCATGAACAA GAGCGCCGCC GCTGGCCTGC TGGGCTATGC CCGCGTCAGC ACCGACGACC AGGACTTGAC CAACCAACGG GCCGAACTGC ACGCGGCCGG CTGCACCAAG CTGTTTTCCG AGAAGATCAC CGGCACCAGG CGCGACCGCC CGGAGCTGGC CAGGATGCTT GACCACCTAC GCCCTGGCGA CGTTGTGACA GTGACCAGGC TAGACCGCCT GGCCCGCAGC ACCCGCGACC TACTGGACAT TGCCGAGCGC ATCCAGGAGG CCGGCGCGGG CCTGCGTAGC CTGGCAGAGC CGTGGGCCGA CACCACCACG CCGGCCGGCC GCATGGTGTT GACCGTGTTC GCCGGCATTG CCGAGTTCGA GCGTTCCCTA ATCATCGACC GCACCCGGAG CGGGCGCGAG GCCGCCAAGG CCCGAGGCGT GAAGTTTGGC CCCCGCCCTA CCCTCACCCC GGCACAGATC GCGCACGCCC GCGAGCTGAT CGACCAGGAA GGCCGCACCG TGAAAGAGGC GGCTGCACTG CTTGGCGTGC ATCGCTCGAC CCTGTACCGC GCACTTGAGC GCAGCGAGGA AGTGACGCCC ACCGAGGCCA GGCGGCGCGG TGCCTTCCGT GAGGACGCAT TGACCGAGGC CGACGCCCTG GCGGCCGCCG AGAATGAACG CCAAGAGGAA CAAGCATGAA ACCGCACCAG GACGGCCAGG ACGAACCGTT TTTCATTACC GAAGAGATCG AGGCGGAGAT GATCGCGGCC GGGTACGTGT TCGAGCCGCC CGCGCACGTC TCAACCGTGC GGCTGCATGA AATCCTGGCC GGTTTGTCTG ATGCCAAGCT GGCGGCCTGG CCGGCCAGCT TGGCCGCTGA AGAAACCGAG CGCCGCCGTC TAAAAAGGTG ATGTGTATTT GAGTAAAACA GCTTGCGTCA TGCGGTCGCT GCGTATATGA TGCGATGAGT AAATAAACAA ATACGCAAGG GGAACGCATG AAGGTTATCG CTGTACTTAA CCAGAAAGGC GGGTCAGGCA AGACGACCAT CGCAACCCAT CTAGCCCGCG CCCTGCAACT CGCCGGGGCC GATGTTCTGT TAGTCGATTC CGATCCCCAG GGCAGTGCCC GCGATTGGGC GGCCGTGCGG GAAGATCAAC CGCTAACCGT TGTCGGCATC GACCGCCCGA CGATTGACCG CGACGTGAAG GCCATCGGCC GGCGCGACTT CGTAGTGATC GACGGAGCGC CCCAGGCGGC GGACTTGGCT GTGTCCGCGA TCAAGGCAGC CGACTTCGTG CTGATTCCGG TGCAGCCAAG CCCTTACGAC ATATGGGCCA CCGCCGACCT GGTGGAGCTG GTTAAGCAGC GCATTGAGGT CACGGATGGA AGGCTACAAG CGGCCTTTGT CGTGTCGCGG GCGATCAAAG GCACGCGCAT CGGCGGTGAG GTTGCCGAGG CGCTGGCCGG GTACGAGCTG CCCATTCTTG AGTCCCGTAT CACGCAGCGC GTGAGCTACC CAGGCACTGC CGCCGCCGGC ACAACCGTTC TTGAATCAGA ACCCGAGGGC GACGCTGCCC GCGAGGTCCA GGCGCTGGCC GCTGAAATTA AATCAAAACT CATTTGAGTT AATGAGGTAA AGAGAAAATG AGCAAAAGCA CAAACACGCT AAGTGCCGGC CGTCCGAGCG CACGCAGCAG CAAGGCTGCA ACGTTGGCCA GCCTGGCAGA CACGCCAGCC ATGAAGCGGG TCAACTTTCA GTTGCCGGCG GAGGATCACA CCAAGCTGAA GATGTACGCG GTACGCCAAG GCAAGACCAT TACCGAGCTG CTATCTGAAT ACATCGCGCA GCTACCAGAG TAAATGAGCA AATGAATAAA TGAGTAGATG AATTTTAGCG GCTAAAGGAG GCGGCATGGA AAATCAAGAA CAACCAGGCA CCGACGCCGT GGAATGCCCC ATGTGTGGAG GAACGGGCGG TTGGCCAGGC GTAAGCGGCT GGGTTGTCTG CCGGCCCTGC AATGGCACTG GAACCCCCAA GCCCGAGGAA TCGGCGTGAC GGTCGCAAAC CATCCGGCCC GGTACAAATC GGCGCGGCGC TGGGTGATGA CCTGGTGGAG AAGTTGAAGG CCGCGCAGGC CGCCCAGCGG CAACGCATCG AGGCAGAAGC ACGCCCCGGT GAATCGTGGC AAGCGGCCGC TGATCGAATC CGCAAAGAAT CCCGGCAACC GCCGGCAGCC GGTGCGCCGT CGATTAGGAA GCCGCCCAAG GGCGACGAGC AACCAGATTT TTTCGTTCCG ATGCTCTATG ACGTGGGCAC CCGCGATAGT CGCAGCATCA TGGACGTGGC CGTTTTCCGT CTGTCGAAGC GTGACCGACG AGCTGGCGAG GTGATCCGCT ACGAGCTTCC AGACGGGCAC GTAGAGGTTT CCGCAGGGCC GGCCGGCATG GCCAGTGTGT GGGATTACGA CCTGGTACTG ATGGCGGTTT CCCATCTAAC CGAATCCATG AACCGATACC GGGAAGGGAA GGGAGACAAG CCCGGCCGCG TGTTCCGTCC ACACGTTGCG GACGTACTCA AGTTCTGCCG GCGAGCCGAT GGCGGAAAGC AGAAAGACGA CCTGGTAGAA ACCTGCATTC GGTTAAACAC CACGCACGTT GCCATGCAGC GTACGAAGAA GGCCAAGAAC GGCCGCCTGG TGACGGTATC CGAGGGTGAA GCCTTGATTA GCCGCTACAA GATCGTAAAG AGCGAAACCG GGCGGCCGGA GTACATCGAG ATCGAGCTAG CTGATTGGAT GTACCGCGAG ATCACAGAAG GCAAGAACCC GGACGTGCTG ACGGTTCACC CCGATTACTT TTTGATCGAT CCCGGCATCG GCCGTTTTCT CTACCGCCTG GCACGCCGCG CCGCAGGCAA GGCAGAAGCC AGATGGTTGT TCAAGACGAT CTACGAACGC AGTGGCAGCG CCGGAGAGTT CAAGAAGTTC TGTTTCACCG TGCGCAAGCT GATCGGGTCA AATGACCTGC CGGAGTACGA TTTGAAGGAG GAGGCGGGGC AGGCTGGCCC GATCCTAGTC ATGCGCTACC GCAACCTGAT CGAGGGCGAA GCATCCGCCG GTTCCTAATG TACGGAGCAG ATGCTAGGGC AAATTGCCCT AGCAGGGGAA AAAGGTCGAA AAGGTCTCTT TCCTGTGGAT AGCACGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGAACCCGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGTCACAC ATGTAAGTGA CTGATATAAA AGAGAAAAAA GGCGATTTTT CCGCCTAAAA CTCTTTAAAA CTTATTAAAA CTCTTAAAAC CCGCCTGGCC TGTGCATAAC TGTCTGGCCA GCGCACAGCC GAAGAGCTGC AAAAAGCGCC TACCCTTCGG TCGCTGCGCT CCCTACGCCC CGCCGCTTCG CGTCGGCCTA TCGCGGCCGC TGGCCGCTCA AAAATGGCTG GCCTACGGCC AGGCAATCTA CCAGGGCGCG GACAAGCCGC GCCGTCGCCA CTCGACCGCC GGCGCCCACA TCAAGGCACC CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GCATTCTAGG TACTAAAACA ATTCATCCAG TAAAATATAA TATTTTATTT TCTCCCAATC AGGCTTGATC CCCAGTAAGT CAAAAAATAG CTCGACATAC TGTTCTTCCC CGATATCCTC CCTGATCGAC CGGACGCAGA AGGCAATGTC ATACCACTTG TCCGCCCTGC CGCTTCTCCC AAGATCAATA AAGCCACTTA CTTTGCCATC TTTCACAAAG ATGTTGCTGT CTCCCAGGTC GCCGTGGGAA AAGACAAGTT CCTCTTCGGG CTTTTCCGTC TTTAAAAAAT CATACAGCTC GCGCGGATCT TTAAATGGAG TGTCTTCTTC CCAGTTTTCG CAATCCACAT CGGCCAGATC GTTATTCAGT AAGTAATCCA ATTCGGCTAA GCGGCTGTCT AAGCTATTCG TATAGGGACA ATCCGATATG TCGATGGAGT GAAAGAGCCT GATGCACTCC GCATACAGCT CGATAATCTT TTCAGGGCTT TGTTCATCTT CATACTCTTC CGAGCAAAGG ACGCCATCGG CCTCACTCAT GAGCAGATTG CTCCAGCCAT CATGCCGTTC AAAGTGCAGG ACCTTTGGAA CAGGCAGCTT TCCTTCCAGC CATAGCATCA TGTCCTTTTC CCGTTCCACA TCATAGGTGG TCCCTTTATA CCGGCTGTCC GTCATTTTTA AATATAGGTT TTCATTTTCT CCCACCAGCT TATATACCTT AGCAGGAGAC ATTCCTTCCG TATCTTTTAC GCAGCGGTAT TTTTCGATCA GTTTTTTCAA TTCCGGTGAT ATTCTCATTT TAGCCATTTA TTATTTCCTT CCTCTTTTCT ACAGTATTTA AAGATACCCC AAGAAGCTAA TTATAACAAG ACGAACTCCA ATTCACTGTT CCTTGCATTC TAAAACCTTA AATACCAGAA AACAGCTTTT TCAAAGTTGT TTTCAAAGTT GGCGTATAAC ATAGTATCGA CGGAGCCGAT TTTGAAACCG CGGTGATCAC AGGCAGCAAC GCTCTGTCAT CGTTACAATC AACATGCTAC CCTCCGCGAG ATCATCCGTG TTTCAAACCC GGCAGCTTAG TTGCCGTTCT TCCGAATAGC ATCGGTAACA TGAGCAAAGT CTGCCGCCTT ACAACGGCTC TCCCGCTGAC GCCGTCCCGG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGA ATTAACGCCG AATTAATTCG GGGGATCTGG ATTTTAGTAC TGGATTTTGG TTTTAGGAAT TAGAAATTTT ATTGATAGAA GTATTTTACA AATACAAATA CATACTAAGG GTTTCTTATA TGCTCAACAC ATGAGCGAAA CCCTATAGGA ACCCTAATTC CCTTATCTGG GAACTACTCA CACATTATTA TGGAGAAACT TGGCAA
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