pCAMBIA1300-AtU6-26-BRI1(arabidopsis)-sgRNA1-pYAO-Cas9
$113.00
In stock
SKU
PL56980
100μg liquid plasmid(Instantly):$156
| name | pCAMBIA1300-AtU6-26-BRI1(arabidopsis)-sgRNA1-pYAO-Cas9 | another name | apCAMBIA1300-AtU6-26-BRI1(arabidopsis)-sgRNA1-pYAO-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Arabidopsis | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | ATU6 | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGCTAG AGCAGCTTGA GCTTGGATCA GATTGTCGTT TCCCGCCTTC AGTTTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA GAAAAGAGCG TTTATTAGAA TAACGGATAT TTAAAAGGGC GTGAAAAGGT TTATCCGTTC GTCCATTTGT ATGTGCATGC CAACCACAGG GTTCCCCTCG GGATCAAAGT ACTTTGATCC AACCCCTCCG CTGCTATAGT GCAGTCGGCT TCTGACGTTC AGTGCAGCCG TCTTCTGAAA ACGACATGTC GCACAAGTCC TAAGTTACGC GACAGGCTGC CGCCCTGCCC TTTTCCTGGC GTTTTCTTGT CGCGTGTTTT AGTCGCATAA AGTAGAATAC TTGCGACTAG AACCGGAGAC ATTACGCCAT GAACAAGAGC GCCGCCGCTG GCCTGCTGGG CTATGCCCGC GTCAGCACCG ACGACCAGGA CTTGACCAAC CAACGGGCCG AACTGCACGC GGCCGGCTGC ACCAAGCTGT TTTCCGAGAA GATCACCGGC ACCAGGCGCG ACCGCCCGGA GCTGGCCAGG ATGCTTGACC ACCTACGCCC TGGCGACGTT GTGACAGTGA CCAGGCTAGA CCGCCTGGCC CGCAGCACCC GCGACCTACT GGACATTGCC GAGCGCATCC AGGAGGCCGG CGCGGGCCTG CGTAGCCTGG CAGAGCCGTG GGCCGACACC ACCACGCCGG CCGGCCGCAT GGTGTTGACC GTGTTCGCCG GCATTGCCGA GTTCGAGCGT TCCCTAATCA TCGACCGCAC CCGGAGCGGG CGCGAGGCCG CCAAGGCCCG AGGCGTGAAG TTTGGCCCCC GCCCTACCCT CACCCCGGCA CAGATCGCGC ACGCCCGCGA GCTGATCGAC CAGGAAGGCC GCACCGTGAA AGAGGCGGCT GCACTGCTTG GCGTGCATCG CTCGACCCTG TACCGCGCAC TTGAGCGCAG CGAGGAAGTG ACGCCCACCG AGGCCAGGCG GCGCGGTGCC TTCCGTGAGG ACGCATTGAC CGAGGCCGAC GCCCTGGCGG CCGCCGAGAA TGAACGCCAA GAGGAACAAG CATGAAACCG CACCAGGACG GCCAGGACGA ACCGTTTTTC ATTACCGAAG AGATCGAGGC GGAGATGATC GCGGCCGGGT ACGTGTTCGA GCCGCCCGCG CACGTCTCAA CCGTGCGGCT GCATGAAATC CTGGCCGGTT TGTCTGATGC CAAGCTGGCG GCCTGGCCGG CCAGCTTGGC CGCTGAAGAA ACCGAGCGCC GCCGTCTAAA AAGGTGATGT GTATTTGAGT AAAACAGCTT GCGTCATGCG GTCGCTGCGT ATATGATGCG ATGAGTAAAT AAACAAATAC GCAAGGGGAA CGCATGAAGG TTATCGCTGT ACTTAACCAG AAAGGCGGGT CAGGCAAGAC GACCATCGCA ACCCATCTAG CCCGCGCCCT GCAACTCGCC GGGGCCGATG TTCTGTTAGT CGATTCCGAT CCCCAGGGCA GTGCCCGCGA TTGGGCGGCC GTGCGGGAAG ATCAACCGCT AACCGTTGTC GGCATCGACC GCCCGACGAT TGACCGCGAC GTGAAGGCCA TCGGCCGGCG CGACTTCGTA GTGATCGACG GAGCGCCCCA GGCGGCGGAC TTGGCTGTGT CCGCGATCAA GGCAGCCGAC TTCGTGCTGA TTCCGGTGCA GCCAAGCCCT TACGACATAT GGGCCACCGC CGACCTGGTG GAGCTGGTTA AGCAGCGCAT TGAGGTCACG GATGGAAGGC TACAAGCGGC CTTTGTCGTG TCGCGGGCGA TCAAAGGCAC GCGCATCGGC GGTGAGGTTG CCGAGGCGCT GGCCGGGTAC GAGCTGCCCA TTCTTGAGTC CCGTATCACG CAGCGCGTGA GCTACCCAGG CACTGCCGCC GCCGGCACAA CCGTTCTTGA ATCAGAACCC GAGGGCGACG CTGCCCGCGA GGTCCAGGCG CTGGCCGCTG AAATTAAATC AAAACTCATT TGAGTTAATG AGGTAAAGAG AAAATGAGCA AAAGCACAAA CACGCTAAGT GCCGGCCGTC CGAGCGCACG CAGCAGCAAG GCTGCAACGT TGGCCAGCCT GGCAGACACG CCAGCCATGA AGCGGGTCAA CTTTCAGTTG CCGGCGGAGG ATCACACCAA GCTGAAGATG TACGCGGTAC GCCAAGGCAA GACCATTACC GAGCTGCTAT CTGAATACAT CGCGCAGCTA CCAGAGTAAA TGAGCAAATG AATAAATGAG TAGATGAATT TTAGCGGCTA AAGGAGGCGG CATGGAAAAT CAAGAACAAC CAGGCACCGA CGCCGTGGAA TGCCCCATGT GTGGAGGAAC GGGCGGTTGG CCAGGCGTAA GCGGCTGGGT TGTCTGCCGG CCCTGCAATG GCACTGGAAC CCCCAAGCCC GAGGAATCGG CGTGACGGTC GCAAACCATC CGGCCCGGTA CAAATCGGCG CGGCGCTGGG TGATGACCTG GTGGAGAAGT TGAAGGCCGC GCAGGCCGCC CAGCGGCAAC GCATCGAGGC AGAAGCACGC CCCGGTGAAT CGTGGCAAGC GGCCGCTGAT CGAATCCGCA AAGAATCCCG GCAACCGCCG GCAGCCGGTG CGCCGTCGAT TAGGAAGCCG CCCAAGGGCG ACGAGCAACC AGATTTTTTC GTTCCGATGC TCTATGACGT GGGCACCCGC GATAGTCGCA GCATCATGGA CGTGGCCGTT TTCCGTCTGT CGAAGCGTGA CCGACGAGCT GGCGAGGTGA TCCGCTACGA GCTTCCAGAC GGGCACGTAG AGGTTTCCGC AGGGCCGGCC GGCATGGCCA GTGTGTGGGA TTACGACCTG GTACTGATGG CGGTTTCCCA TCTAACCGAA TCCATGAACC GATACCGGGA AGGGAAGGGA GACAAGCCCG GCCGCGTGTT CCGTCCACAC GTTGCGGACG TACTCAAGTT CTGCCGGCGA GCCGATGGCG GAAAGCAGAA AGACGACCTG GTAGAAACCT GCATTCGGTT AAACACCACG CACGTTGCCA TGCAGCGTAC GAAGAAGGCC AAGAACGGCC GCCTGGTGAC GGTATCCGAG GGTGAAGCCT TGATTAGCCG CTACAAGATC GTAAAGAGCG AAACCGGGCG GCCGGAGTAC ATCGAGATCG AGCTAGCTGA TTGGATGTAC CGCGAGATCA CAGAAGGCAA GAACCCGGAC GTGCTGACGG TTCACCCCGA TTACTTTTTG ATCGATCCCG GCATCGGCCG TTTTCTCTAC CGCCTGGCAC GCCGCGCCGC AGGCAAGGCA GAAGCCAGAT GGTTGTTCAA GACGATCTAC GAACGCAGTG GCAGCGCCGG AGAGTTCAAG AAGTTCTGTT TCACCGTGCG CAAGCTGATC GGGTCAAATG ACCTGCCGGA GTACGATTTG AAGGAGGAGG CGGGGCAGGC TGGCCCGATC CTAGTCATGC GCTACCGCAA CCTGATCGAG GGCGAAGCAT CCGCCGGTTC CTAATGTACG GAGCAGATGC TAGGGCAAAT TGCCCTAGCA GGGGAAAAAG GTCGAAAAGG TCTCTTTCCT GTGGATAGCA CGTACATTGG GAACCCAAAG CCGTACATTG GGAACCGGAA CCCGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG TCACACATGT AAGTGACTGA TATAAAAGAG AAAAAAGGCG ATTTTTCCGC CTAAAACTCT TTAAAACTTA TTAAAACTCT TAAAACCCGC CTGGCCTGTG CATAACTGTC TGGCCAGCGC ACAGCCGAAG AGCTGCAAAA AGCGCCTACC CTTCGGTCGC TGCGCTCCCT ACGCCCCGCC GCTTCGCGTC GGCCTATCGC GGCCGCTGGC CGCTCAAAAA TGGCTGGCCT ACGGCCAGGC AATCTACCAG GGCGCGGACA AGCCGCGCCG TCGCCACTCG ACCGCCGGCG CCCACATCAA GGCACCCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGCAT TCTAGGTACT AAAACAATTC ATCCAGTAAA ATATAATATT TTATTTTCTC CCAATCAGGC TTGATCCCCA GTAAGTCAAA AAATAGCTCG ACATACTGTT CTTCCCCGAT ATCCTCCCTG ATCGACCGGA CGCAGAAGGC AATGTCATAC CACTTGTCCG CCCTGCCGCT TCTCCCAAGA TCAATAAAGC CACTTACTTT GCCATCTTTC ACAAAGATGT TGCTGTCTCC CAGGTCGCCG TGGGAAAAGA CAAGTTCCTC TTCGGGCTTT TCCGTCTTTA AAAAATCATA CAGCTCGCGC GGATCTTTAA ATGGAGTGTC TTCTTCCCAG TTTTCGCAAT CCACATCGGC CAGATCGTTA TTCAGTAAGT AATCCAATTC GGCTAAGCGG CTGTCTAAGC TATTCGTATA GGGACAATCC GATATGTCGA TGGAGTGAAA GAGCCTGATG CACTCCGCAT ACAGCTCGAT AATCTTTTCA GGGCTTTGTT CATCTTCATA CTCTTCCGAG CAAAGGACGC CATCGGCCTC ACTCATGAGC AGATTGCTCC AGCCATCATG CCGTTCAAAG TGCAGGACCT TTGGAACAGG CAGCTTTCCT TCCAGCCATA GCATCATGTC CTTTTCCCGT TCCACATCAT AGGTGGTCCC TTTATACCGG CTGTCCGTCA TTTTTAAATA TAGGTTTTCA TTTTCTCCCA CCAGCTTATA TACCTTAGCA GGAGACATTC CTTCCGTATC TTTTACGCAG CGGTATTTTT CGATCAGTTT TTTCAATTCC GGTGATATTC TCATTTTAGC CATTTATTAT TTCCTTCCTC TTTTCTACAG TATTTAAAGA TACCCCAAGA AGCTAATTAT AACAAGACGA ACTCCAATTC ACTGTTCCTT GCATTCTAAA ACCTTAAATA CCAGAAAACA GCTTTTTCAA AGTTGTTTTC AAAGTTGGCG TATAACATAG TATCGACGGA GCCGATTTTG AAACCGCGGT GATCACAGGC AGCAACGCTC TGTCATCGTT ACAATCAACA TGCTACCCTC CGCGAGATCA TCCGTGTTTC AAACCCGGCA GCTTAGTTGC CGTTCTTCCG AATAGCATCG GTAACATGAG CAAAGTCTGC CGCCTTACAA CGGCTCTCCC GCTGACGCCG TCCCGGACTG ATGGGCTGCC TGTATCGAGT GGTGATTTTG TGCCGAGCTG CCGGTCGGGG AGCTGTTGGC TGGCTGGTGG CAGGATATAT TGTGGTGTAA ACAAATTGAC GCTTAGACAA CTTAATAACA CATTGCGGAC GTTTTTAATG TACTGAATTA ACGCCGAATT AATTCGGGGG ATCTGGATTT TAGTACTGGA TTTTGGTTTT AGGAATTAGA AATTTTATTG ATAGAAGTAT TTTACAAATA CAAATACATA CTAAGGGTTT CTTATATGCT CAACACATGA GCGAAACCCT ATAGGAACCC TAATTCCCTT ATCTGGGAAC TACTCACACA TTATTATGGA GAAACTCGAG CTTGTCGATC GACAGATCCG GTCGGCATCT ACTCTATTTC TTTGCCCTCG GACGAGTGCT GGGGCGTCGG TTTCCACTAT CGGCGAGTAC TTCTACACAG CCATCGGTCC AGACGGCCGC GCTTCTGCGG GCGATTTGTG TACGCCCGAC AGTCCCGGCT CCGGATCGGA CGATTGCGTC GCATCGACCC TGCGCCCAAG CTGCATCATC GAAATTGCCG TCAACCAAGC TCTGATAGAG TTGGTCAAGA CCAATGCGGA GCATATACGC CCGGAGTCGT GGCGATCCTG CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC GCGTCTGCTG CTCCATACAA GCCAACCACG GCCTCCAGAA GAAGATGTTG GCGACCTCGT ATTGGGAATC CCCGAACATC GCCTCGCTCC AGTCAATGAC CGCTGTTATG CGGCCATTGT CCGTCAGGAC ATTGTTGGAG CCGAAATCCG CGTGCACGAG GTGCCGGACT TCGGGGCAGT CCTCGGCCCA AAGCATCAGC TCATCGAGAG CCTGCGCGAC GGACGCACTG ACGGTGTCGT CCATCACAGT TTGCCAGTGA TACACATGGG GATCAGCAAT CGCGCATATG AAATCACGCC ATGTAGTGTA TTGACCGATT CCTTGCGGTC CGAATGGGCC GAACCCGCTC GTCTGGCTAA GATCGGCCGC AGCGATCGCA TCCATAGCCT CCGCGACCGG TTGTAGAACA GCGGGCAGTT CGGTTTCAGG CAGGTCTTGC AACGTGACAC CCTGTGCACG GCGGGAGATG CAATAGGTCA GGCTCTCGCT AAACTCCCCA ATGTCAAGCA CTTCCGGAAT CGGGAGCGCG GCCGATGCAA AGTGCCGATA AACATAACGA TCTTTGTAGA AACCATCGGC GCAGCTATTT ACCCGCAGGA CATATCCACG CCCTCCTACA TCGAAGCTGA AAGCACGAGA TTCTTCGCCC TCCGAGAGCT GCATCAGGTC GGAGACGCTG TCGAACTTTT CGATCAGAAA CTTCTCGACA GACGTCGCGG TGAGTTCAGG CTTTTTCATA TCTCATTGCC CCCCGGGATC TGCGAAAGCT CGAGAGAGAT AGATTTGTAG AGAGAGACTG GTGATTTCAG CGTGTCCTCT CCAAATGAAA TGAACTTCCT TATATAGAGG AAGGTCTTGC GAAGGATAGT GGGATTGTGC GTCATCCCTT ACGTCAGTGG AGATATCACA TCAATCCACT TGCTTTGAAG ACGTGGTTGG AACGTCTTCT TTTTCCACGA TGCTCCTCGT GGGTGGGGGT CCATCTTTGG GACCACTGTC GGCAGAGGCA TCTTGAACGA TAGCCTTTCC TTTATCGCAA TGATGGCATT TGTAGGTGCC ACCTTCCTTT TCTACTGTCC TTTTGATGAA GTGACAGATA GCTGGGCAAT GGAATCCGAG GAGGTTTCCC GATATTACCC TTTGTTGAAA AGTCTCAATA GCCCTTTGGT CTTCTGAGAC TGTATCTTTG ATATTCTTGG AGTAGACGAG AGTGTCGTGC TCCACCATGT TATCACATCA ATCCACTTGC TTTGAAGACG TGGTTGGAAC GTCTTCTTTT TCCACGATGC TCCTCGTGGG TGGGGGTCCA TCTTTGGGAC CACTGTCGGC AGAGGCATCT TGAACGATAG CCTTTCCTTT ATCGCAATGA TGGCATTTGT AGGTGCCACC TTCCTTTTCT ACTGTCCTTT TGATGAAGTG ACAGATAGCT GGGCAATGGA ATCCGAGGAG GTTTCCCGAT ATTACCCTTT GTTGAAAAGT CTCAATAGCC CTTTGGTCTT CTGAGACTGT ATCTTTGATA TTCTTGGAGT AGACGAGAGT GTCGTGCTCC ACCATGTTGG CAAGCTGCTC TAGCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACA TGATTACGAA TTCCCGATCT AGTAACATAG ATGACACCGC GCGCGATAAT TTATCCTAGT TTGCGCGCTA TATTTTGTTT TCTATCGCGT ATTAAATGTA TAATTGCGGG ACTCTAATCA TAAAAACCCA TCTCATAAAT AACGTCATGC ATTACATGTT AATTATTACA TGCTTAACGT AATTCAACAG AAATTATATG ATAATCATCG CAAGACCGGC AACAGGATTC AATCTTAAGA AACTTTATTG CCAAATGTTT GAACGATCGG GGAAATTCGA GCTCTATCGA TCAATCAGGA TCCTTACTTT TTCTTTTTTG CCTGGCCGGC CTTTTTCGTG GCCGCCGGCC TTTTGTCGCC TCCCAGCTGA GACAGGTCGA TCCGTGTCTC GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT TGGTGCTGGT GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG CTCCCAGATT GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT TATCCCGGTG CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA CTCTCTTGGA GAACTCGCTG ATCTGCTCGA TGATCTCGTC CAGGTAGTGC TTGTGCTGTT CCACAAACAG CTGTTTCTGC TCATTATCCT CGGGGGAGCC CTTCAGCTTC TCATAGTGGC TGGCCAGGTA CAGGAAGTTC ACATATTTGG AGGGCAGGGC CAGTTCGTTT CCCTTCTGCA GTTCGCCGGC AGAGGCCAGC ATTCTCTTCC GGCCGTTTTC CAGCTCGAAC AGGGAGTACT TAGGCAGCTT GATGATCAGG TCCTTTTTCA CTTCTTTGTA GCCCTTGGCT TCCAGAAAGT CGATGGGATT CTTCTCGAAG CTGCTTCTTT CCATGATGGT GATCCCCAGC AGCTCTTTCA CACTCTTCAG TTTCTTGGAC TTGCCCTTTT CCACTTTGGC CACCACCAGC ACAGAATAGG CCACGGTGGG GCTGTCGAAG CCGCCGTACT TCTTAGGGTC CCAGTCCTTC TTTCTGGCGA TCAGCTTATC GCTGTTCCTC TTGGGCAGGA TAGACTCTTT GCTGAAGCCG CCTGTCTGCA CCTCGGTCTT TTTCACGATA TTCACTTGGG GCATGCTCAG CACTTTCCGC ACGGTGGCAA AATCCCGGCC CTTATCCCAC ACGATCTCCC CGGTTTCGCC GTTTGTCTCG ATCAGAGGCC GCTTCCGGAT CTCGCCGTTG GCCAGGGTAA TCTCGGTCTT GAAAAAGTTC ATGATGTTGC TGTAGAAGAA GTACTTGGCG GTAGCCTTGC CGATTTCCTG CTCGCTCTTG GCGATCATCT TCCGCACGTC GTACACCTTG TAGTCGCCGT ACACGAACTC GCTTTCCAGC TTAGGGTACT TTTTGATCAG GGCGGTTCCC ACGACGGCGT TCAGGTAGGC GTCGTGGGCG TGGTGGTAGT TGTTGATCTC GCGCACTTTG TAAAACTGGA AATCCTTCCG GAAATCGGAC ACCAGCTTGG ACTTCAGGGT GATCACTTTC ACTTCCCGGA TCAGCTTGTC ATTCTCGTCG TACTTAGTGT TCATCCGGGA GTCCAGGATC TGTGCCACGT GCTTTGTGAT CTGCCGGGTT TCCACCAGCT GTCTCTTGAT GAAGCCGGCC TTATCCAGTT CGCTCAGGCC GCCTCTCTCG GCCTTGGTCA GATTGTCGAA CTTTCTCTGG GTAATCAGCT TGGCGTTCAG CAGCTGCCGC CAGTAGTTCT TCATCTTCTT CACGACCTCT TCGGAGGGCA CGTTGTCGCT CTTGCCCCGG TTCTTGTCGC TTCTGGTCAG CACCTTGTTG TCGATGGAGT CGTCCTTCAG AAAGCTCTGA GGCACGATAT GGTCCACATC GTAGTCGGAC AGCCGGTTGA TGTCCAGTTC CTGGTCCACG TACATATCCC GCCCATTCTG CAGGTAGTAC AGGTACAGCT TCTCGTTCTG CAGCTGGGTG TTTTCCACGG GGTGTTCTTT CAGGATCTGG CTGCCCAGCT CTTTGATGCC CTCTTCGATC CGCTTCATTC TCTCGCGGCT GTTCTTCTGT CCCTTCTGGG TGGTCTGGTT CTCTCTGGCC ATTTCGATCA CGATGTTCTC GGGCTTGTGC CGGCCCATCA CTTTCACGAG CTCGTCCACC ACCTTCACTG TCTGCAGGAT GCCCTTCTTA ATGGCGGGGC TGCCGGCCAG ATTGGCAATG TGCTCGTGCA GGCTATCGCC CTGGCCGGAC ACCTGGGCTT TCTGGATGTC CTCTTTAAAG GTCAGGCTGT CGTCGTGGAT CAGCTGCATG AAGTTTCTGT TGGCGAAGCC GTCGGACTTC AGGAAATCCA GGATTGTCTT GCCGGACTGC TTGTCCCGGA TGCCGTTGAT CAGCTTCCGG CTCAGCCTGC CCCAGCCGGT GTATCTCCGC CGCTTCAGCT GCTTCATCAC TTTGTCGTCG AACAGGTGGG CATAGGTTTT CAGCCGTTCC TCGATCATCT CTCTGTCCTC AAACAGTGTC AGGGTCAGCA CGATATCTTC CAGAATGTCC TCGTTTTCCT CATTGTCCAG GAAGTCCTTG TCCTTGATAA TTTTCAGCAG ATCGTGGTAT GTGCCCAGGG AGGCGTTGAA CCGATCTTCC ACGCCGGAGA TTTCCACGGA GTCGAAGCAC TCGATTTTCT TGAAGTAGTC CTCTTTCAGC TGCTTCACGG TCACTTTCCG GTTGGTCTTG AACAGCAGGT CCACGATGGC CTTTTTCTGC TCGCCGCTCA GGAAGGCGGG CTTTCTCATT CCCTCGGTCA CGTATTTCAC TTTGGTCAGC TCGTTATACA CGGTGAAGTA CTCGTACAGC AGGCTGTGCT TGGGCAGCAC CTTCTCGTTG GGCAGGTTCT TATCGAAGTT GGTCATCCGC TCGATGAAGC TCTGGGCGGA AGCGCCCTTG TCCACCACTT CCTCGAAGTT CCAGGGGGTG ATGGTTTCCT CGCTCTTTCT GGTCATCCAG GCGAATCTGC TGTTTCCCCT GGCCAGAGGG CCCACGTAGT AGGGGATGCG GAAGGTCAGG ATCTTCTCGA TCTTTTCCCG GTTGTCCTTC AGGAATGGGT AAAAATCTTC CTGCCGCCGC AGAATGGCGT GCAGCTCTCC CAGGTGGATC TGGTGGGGGA TGCTGCCGTT GTCGAAGGTC CGCTGCTTCC GCAGCAGGTC CTCTCTGTTC AGCTTCACGA GCAGTTCCTC GGTGCCGTCC ATCTTTTCCA GGATGGGCTT GATGAACTTG TAGAACTCTT CCTGGCTGGC TCCGCCGTCA ATGTAGCCGG CGTAGCCGTT CTTGCTCTGG TCGAAGAAAA TCTCTTTGTA CTTCTCAGGC AGCTGCTGCC GCACGAGAGC TTTCAGCAGG GTCAGGTCCT GGTGGTGCTC GTCGTATCTC TTGATCATAG AGGCGCTCAG GGGGGCCTTG GTGATCTCGG TGTTCACTCT CAGGATGTCG CTCAGCAGGA TGGCGTCGGA CAGGTTCTTG GCGGCCAGAA ACAGGTCGGC GTACTGGTCG CCGATCTGGG CCAGCAGGTT GTCCAGGTCG TCGTCGTAGG TGTCCTTGCT CAGCTGCAGT TTGGCATCCT CGGCCAGGTC GAAGTTGCTC TTGAAGTTGG GGGTCAGGCC CAGGCTCAGG GCAATCAGGT TTCCGAACAG GCCATTCTTC TTCTCGCCGG GCAGCTGGGC GATCAGATTT TCCAGCCGTC TGCTCTTGCT CAGTCTGGCA GACAGGATGG CCTTGGCGTC CACGCCGCTG GCGTTGATGG GGTTTTCCTC GAACAGCTGG TTGTAGGTCT GCACCAGCTG GATGAACAGC TTGTCCACGT CGCTGTTGTC GGGGTTCAGG TCGCCCTCGA TCAGGAAGTG GCCCCGGAAC TTGATCATGT GGGCCAGGGC CAGATAGATC AGCCGCAGGT CGGCCTTGTC GGTGCTGTCC ACCAGTTTCT TTCTCAGGTG GTAGATGGTG GGGTACTTCT CGTGGTAGGC CACCTCGTCC ACGATGTTGC CGAAGATGGG GTGCCGCTCG TGCTTCTTAT CCTCTTCCAC CAGGAAGGAC TCTTCCAGTC TGTGGAAGAA GCTGTCGTCC ACCTTGGCCA TCTCGTTGCT GAAGATCTCT TGCAGATAGC AGATCCGGTT CTTCCGTCTG GTGTATCTTC TTCTGGCGGT TCTCTTCAGC CGGGTGGCCT CGGCTGTTTC GCCGCTGTCG AACAGCAGGG CTCCGATCAG GTTCTTCTTG ATGCTGTGCC GGTCGGTGTT GCCCAGCACC TTGAATTTCT TGCTGGGCAC CTTGTACTCG TCGGTGATCA CGGCCCAGCC CACAGAGTTG GTGCCGATGT CCAGGCCGAT GCTGTACTTC TTGTCGGCTG CTGGGACTCC GTGGATACCG ACCTTCCGCT TCTTCTTTGG GGCCATCTTA TCGTCATCGT CTTTGTAATC AATATCATGA TCCTTGTAGT CTCCGTCGTG GTCCTTATAG TCCATCTCGA CTCCTTTCTT CTTCTCGTTG TTGTACTTCA TTCTTCTCTC TCTCACTCCC TCTTAGGGTT TAACGATTTT ACACTTTGAC TCGTCTAGGT TTAGCCTCAG GGTTTAGTGG CGACGGAAGA AGCTGGAAAA CGATTTTGTT TATTTGGTCG GAAAGTTTAC AACTTTAAAC CTTCCACTAG GTTTTCTTCA GGAATCGGGT CTACCGGGTT GACCCGGACT AACCAAGCCC ATGACTTACC TGGTCTATGG TTTGAGCCCA TTAACTATTT CGTTCTTTTA AAGCAGAAAC TTGGGCCTTT AATTTGTAGA GCCGAAGACT AACCGGACCG TCAATTTAAG TTTGGTTCAA TCCGATTTCT GAATTTTTGG TTCTGATTTC TGAAGAGTAG TACGTAAACC GAATTGGGAG ATTCTCTTAT TTCAGTAACA GTTTAATACA GTTTTTGTTT AGTGTTTTCA ATTTCGGTAT TTTTTACAAA CTCGAATCGG ATGATTCCGG TTTAATAAAA TCAAATGAGT TCGGTTTAGC TGAATTTAAA ACTGTTTTTA ATCTTATAGT ATACCGAACC GGACCATCCA TCTAATTTCA ATTGTCTACC AAAACCGACC GACCAGTCTT TGTCGAAAGT TGTCTTTCTT CTTCCTTGTT CGATTTTCGC CTCCTTTGGC TTTTCATTCT CTCTCTAACT TCTCTATGTT AAAATTCCGA AAGCTCGATT CAGAAATCTA GAAGCTTCCT CGAGGGCCCT CGCGTAGAAA GTCGAACTCA AATTCCCTGG AGACAAAGAA GAGGAAGATG AGCTCATGGA ACTACGTTGT TACGGCTCAC AAACCGACCA GTGTCACTCA CTCGTGCGTT GGCAACTTCA CAAGTCCTCA AGAGCTCAAT CTCATTGTCG CGTACGAAAC TCTCCCCCTA CCTCTATTTG TTATCAATTT TTGTTTGTCA ATTAGTCCCC TTTTGAATTG CAGCTGTTGA TTTGGGTTTA GGGTTTTCAA TGAATTTCCC ATCGTCGAGG GGGGGCCCGG TACCTTCCTA GGATACTAGT AGTGTGCTGG AATTGCCCTT AAGCTTCGTT GAACAACGGA AACTCGACTT GCCTTCCGCA CAATACATCA TTTCTTCTTA GCTTTTTTTC TTCTTCTTCG TTCATACAGT TTTTTTTTGT TTATCAGCTT ACATTTTCTT GAACCGTAGC TTTCGTTTTC TTCTTTTTAA CTTTCCATTC GGAGTTTTTG TATCTTGTTT CATAGTTTGT CCCAGGATTA GAATGATTAG GCATCGAACC TTCAAGAATT TGATTGAATA AAACATCTTC ATTCTTAAGA TATGAAGATA ATCTTCAAAA GGCCCCTGGG AATCTGAAAG AAGAGAAGCA GGCCCATTTA TATGGGAAAG AACAATAGTA TTTCTTATAT AGGCCCATTT AAGTTGAAAA CAATCTTCAA AAGTCCCACA TCGCTTAGAT AAGAAAACGA AGCTGAGTTT ATATACAGCT AGAGTCGAAG TAGTGATTGT TGGGTCATAA CGATATCTCG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTGTCCCTTC GAAGGGCAAT TCTGCAGATA TCCATCACAC TGGGTCGACC TGCAGGCATG CAAGCTTGGC
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