NM_199460.4;CACH2; CACN2; CACNL1A1; CaV1.2; CCHL1A1;LQT8; TS; TS. LQT8
100μg liquid plasmid(Instantly):$265
| name | PiggyBac-CMV-CACNA1C(human)(1synonymous mutations)-EF1a-Puro | another name | aPiggyBac-CMV-CACNA1C(human)(1synonymous mutations)-EF1a-Puro |
| Insert Size (bp) | 6666bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | EF1a-R：gagccagtacacgacatcac | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGGTCA ATGAGAATAC GAGGATGTAC ATTCCAGAGG AAAACCACCA AGGTTCCAAC TATGGGAGCC CACGCCCCGC CCATGCCAAC ATGAATGCCA ATGCGGCAGC GGGGCTGGCC CCTGAGCACA TCCCCACCCC GGGGGCTGCC CTGTCGTGGC AGGCGGCCAT CGACGCAGCC CGGCAGGCTA AGCTGATGGG CAGCGCTGGC AATGCGACCA TCTCCACAGT CAGCTCCACG CAGCGGAAGC GGCAGCAATA TGGGAAACCC AAGAAGCAGG GCAGCACCAC GGCCACACGC CCGCCCCGAG CCCTGCTCTG CCTGACCCTG AAGAACCCCA TCCGGAGGGC CTGCATCAGC ATTGTCGAAT GGAAACCATT TGAAATAATT ATTTTACTGA CTATTTTTGC CAATTGTGTG GCCTTAGCGA TCTATATTCC CTTTCCAGAA GATGATTCCA ACGCCACCAA TTCCAACCTG GAACGAGTGG AATATCTCTT TCTCATAATT TTTACGGTGG AAGCGTTTTT AAAAGTAATC GCCTATGGAC TCCTCTTTCA CCCCAATGCC TACCTCCGCA ACGGCTGGAA CCTACTAGAT TTTATAATTG TGGTTGTGGG GCTTTTTAGT GCAATTTTAG AACAAGCAAC CAAAGCAGAT GGGGCAAACG CTCTCGGAGG GAAAGGGGCC GGATTTGATG TGAAGGCGCT GAGGGCCTTC CGCGTGCTGC GCCCCCTGCG GCTGGTGTCC GGAGTCCCAA GTCTCCAGGT GGTCCTGAAT TCCATCATCA AGGCCATGGT CCCCCTGCTG CACATCGCCC TGCTTGTGCT GTTTGTCATC ATCATCTACG CCATCATCGG CTTGGAGCTC TTCATGGGGA AGATGCACAA GACCTGCTAC AACCAGGAGG GCATAGCAGA TGTTCCAGCA GAAGATGACC CTTCCCCTTG TGCGCTGGAA ACGGGCCACG GGCGGCAGTG CCAGAACGGC ACGGTGTGCA AGCCCGGCTG GGATGGTCCC AAGCACGGCA TCACCAACTT TGACAACTTT GCCTTCGCCA TGCTCACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC GGACGTGCTG TACTGGGTCA ATGATGCCGT AGGAAGGGAC TGGCCCTGGA TCTATTTTGT TACACTAATC ATCATAGGGT CATTTTTTGT ACTTAACTTG GTTCTCGGTG TGCTTAGCGG AGAGTTTTCC AAAGAGAGGG AGAAGGCCAA GGCCCGGGGA GATTTCCAGA AGCTGCGGGA GAAGCAGCAG CTAGAAGAGG ATCTCAAAGG CTACCTGGAT TGGATCACTC AGGCCGAAGA CATCGATCCT GAGAATGAGG ACGAAGGCAT GGATGAGGAG AAGCCCCGAA ACATGAGCAT GCCCACCAGT GAGACCGAGT CCGTCAACAC CGAAAACGTG GCTGGAGGTG ACATCGAGGG AGAAAACTGC GGGGCCAGGC TGGCCCACCG GATCTCCAAG TCAAAGTTCA GCCGCTACTG GCGCCGGTGG AATCGGTTCT GCAGAAGGAA GTGCCGCGCC GCAGTCAAGT CTAATGTCTT CTACTGGCTG GTGATTTTCC TGGTGTTCCT CAACACGCTC ACCATTGCCT CTGAGCACTA CAACCAGCCC AACTGGCTCA CAGAAGTCCA AGACACGGCA AACAAGGCCC TGCTGGCCCT GTTCACGGCA GAGATGCTCC TGAAGATGTA CAGCCTGGGC CTGCAGGCCT ACTTCGTGTC CCTCTTCAAC CGCTTTGACT GCTTCGTCGT GTGTGGCGGC ATCCTGGAGA CCATCCTGGT GGAGACCAAG ATCATGTCCC CACTGGGCAT CTCCGTGCTC AGATGCGTCC GGCTGCTGAG GATTTTCAAG ATCACGAGGT ACTGGAACTC CTTGAGCAAC CTGGTGGCAT CCTTGCTGAA CTCTGTGCGC TCCATCGCCT CCCTGCTCCT TCTCCTCTTC CTCTTCATCA TCATCTTCTC CCTCCTGGGG ATGCAGCTCT TTGGAGGAAA GTTCAACTTT GATGAGATGC AGACCCGGAG GAGCACATTC GATAACTTCC CCCAGTCCCT CCTCACTGTG TTTCAGATCC TGACCGGGGA GGACTGGAAT TCGGTGATGT ATGATGGGAT CATGGCTTAT GGCGGCCCCT CTTTTCCAGG GATGTTAGTC TGTATTTACT TCATCATCCT CTTCATCTGT GGAAACTATA TCCTACTGAA TGTGTTCTTG GCCATTGCTG TGGACAACCT GGCTGATGCT GAGAGCCTCA CATCTGCCCA AAAGGAGGAG GAAGAGGAGA AGGAGAGAAA GAAGCTGGCC AGGACTGCCA GCCCAGAGAA GAAACAAGAG TTGGTGGAGA AGCCGGCAGT GGGGGAATCC AAGGAGGAGA AGATTGAGCT GAAATCCATC ACGGCTGACG GAGAGTCTCC ACCCGCCACC AAGATCAACA TGGATGACCT CCAGCCCAAT GAAAATGAGG ATAAGAGCCC CTACCCCAAC CCAGAAACTA CAGGAGAAGA GGATGAGGAG GAGCCAGAGA TGCCTGTCGG CCCTCGCCCA CGACCACTCT CTGAGCTTCA CCTTAAGGAA AAGGCAGTGC CCATGCCAGA AGCCAGCGCG TTTTTCATCT TCAGCTCTAA CAACAGGTTT CGCCTCCAGT GCCACCGCAT TGTCAATGAC ACGATCTTCA CCAACCTGAT CCTCTTCTTC ATTCTGCTCA GCAGCATTTC CCTGGCTGCT GAGGACCCGG TCCAGCACAC CTCCTTCAGG AACCATATTC TGTTTTATTT TGATATTGTT TTTACCACCA TTTTCACCAT TGAAATTGCT CTGAAGATCC TAGGCAATGC AGACTATGTC TTCACTAGTA TCTTTACATT AGAAATTATC CTTAAGATGA CTGCTTATGG GGCTTTCTTG CACAAGGGTT CTTTCTGCCG GAACTACTTC AACATCCTGG ACCTGCTGGT GGTCAGCGTG TCCCTCATCT CCTTTGGCAT CCAGTCCAGT GCAATCAATG TCGTGAAGAT CTTGCGAGTC CTGCGAGTAC TCAGGCCCCT GAGGGCCATC AACAGGGCCA AGGGGCTAAA GCATGTGGTT CAGTGTGTGT TTGTCGCCAT CCGGACCATC GGGAACATCG TGATTGTCAC CACCCTGCTG CAGTTCATGT TTGCCTGCAT CGGGGTCCAG CTCTTCAAGG GAAAGCTGTA CACCTGTTCA GACAGTTCCA AGCAGACAGA GGCGGAATGC AAGGGCAACT ACATCACGTA CAAAGACGGG GAGGTTGACC ACCCCATCAT CCAACCCCGC AGCTGGGAGA ACAGCAAGTT TGACTTTGAC AATGTTCTGG CAGCCATGAT GGCCCTCTTC ACCGTCTCCA CCTTCGAAGG GTGGCCAGAG CTGCTGTACC GCTCCATCGA CTCCCACACG GAAGACAAGG GCCCCATCTA CAACTACCGT GTGGAGATCT CCATCTTCTT CATCATCTAC ATCATCATCA TCGCCTTCTT CATGATGAAC ATCTTCGTGG GCTTCGTCAT CGTCACCTTT CAGGAGCAGG GGGAGCAGGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGACAGTGC GTGGAATACG CCCTCAAGGC CCGGCCCCTG CGGAGGTACA TCCCCAAGAA CCAGCACCAG TACAAAGTGT GGTACGTGGT CAACTCCACC TACTTCGAGT ACCTGATGTT CGTCCTCATC CTGCTCAACA CCATCTGCCT GGCCATGCAG CACTACGGCC AGAGCTGCCT GTTCAAAATC GCCATGAACA TCCTCAACAT GCTCTTCACT GGCCTCTTCA CCGTGGAGAT GATCCTGAAG CTCATTGCCT TCAAACCCAA GGGTTACTTT AGTGATCCCT GGAATGTTTT TGACTTCCTC ATCGTAATTG GCAGCATAAT TGACGTCATT CTCAGTGAGA CTAATCACTA TTTCTGTGAT GCATGGAATA CATTTGACGC CTTGATTGTT GTGGGTAGCA TTGTTGATAT AGCAATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC TCTCCCTCTA TGAACGCAGA GGAAAACTCC CGCATCTCCA TCACCTTCTT CCGCCTGTTC CGGGTCATGC GTCTGGTGAA GCTGCTGAGC CGTGGGGAGG GCATCCGGAC GCTGCTGTGG ACCTTCATCA AGTCCTTCCA GGCCCTGCCC TATGTGGCCC TCCTGATCGT GATGCTGTTC TTCATCTACG CGGTGATCGG GATGCAGGTG TTTGGGAAAA TTGCCCTGAA TGATACCACA GAGATCAACC GGAACAACAA CTTTCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG TGTGCCACCG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCAGG CAAGAAGTGT GCCCCAGAGT CCGAGCCCAG CAACAGCACG GAGGGTGAAA CACCCTGTGG TAGCAGCTTT GCTGTCTTCT ACTTCATCAG CTTCTACATG CTCTGTGCCT TCCTGATCAT CAACCTCTTT GTAGCTGTCA TCATGGACAA CTTTGACTAC CTGACAAGGG ACTGGTCCAT CCTTGGTCCC CACCACCTGG ATGAGTTTAA AAGAATCTGG GCAGAGTATG ACCCTGAAGC CAAGGGTCGT ATCAAACACC TGGATGTGGT GACCCTCCTC CGGCGGATTC AGCCGCCACT AGGTTTTGGG AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCTCTGAAC AGCGACGGGA CAGTCATGTT CAATGCCACC CTGTTTGCCC TGGTCAGGAC GGCCCTGAGG ATCAAAACAG AAGGGAACCT AGAACAAGCC AATGAGGAGC TGCGGGCGAT CATCAAGAAG ATCTGGAAGC GGACCAGCAT GAAGCTGCTG GACCAGGTGG TGCCCCCTGC AGGTGATGAT GAGGTCACCG TTGGCAAGTT CTACGCCACG TTCCTGATCC AGGAGTACTT CCGGAAGTTC AAGAAGCGCA AAGAGCAGGG CCTTGTGGGC AAGCCCTCCC AGAGGAACGC GCTGTCTCTG CAGGCTGGCT TGCGCACACT GCATGACATC GGGCCTGAGA TCCGACGGGC CATCTCTGGA GATCTCACCG CTGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC CGCTGCTTCT GAAGATGACA TCTTCAGGAG GGCCGGTGGC CTGTTCGGCA ACCACGTCAG CTACTACCAA AGCGACGGCC GGAGCGCCTT CCCCCAGACC TTCACCACTC AGCGCCCGCT GCACATCAAC AAGGCGGGCA GCAGCCAGGG CGACACTGAG TCGCCATCCC ACGAGAAGCT GGTGGACTCC ACCTTCACCC CGAGCAGCTA CTCGTCCACC GGCTCCAACG CCAACATCAA CAACGCCAAC AACACCGCCC TGGGTCGCCT CCCTCGCCCC GCCGGCTACC CCAGCACGGT CAGCACTGTG GAGGGCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGTGGC GTGGAAGCTC AGCTCCAACA GGGAAAGGCA CGTTCCGATG TGTGAGGATC TGGAGCTCAG GAGGGATTCA GGCTCAGCAG GGACTCAGGC TCACTGCCTT CTGCTCAGGA GAGCAAACCC CTCTAGGTGC CACTCCCGGG AGAGCCAGGC AGCCATGGCG GGTCAGGAGG AGACGTCTCA GGATGAGACC TATGAAGTGA AGATGAACCA TGACACGGAG GCCTGCAGTG AGCCCAGCCT GCTCTCCACA GAGATGCTCT CCTACCAGGA TGACGAAAAT CGGCAACTGA CGCTCCCAGA GGAGGACAAG AGGGACATCC GGCAATCTCC GAAGAGGGGT TTCCTCCGCT CTGCCTCACT AGGTCGAAGG GCCTCCTTCC ACCTGGAATG TCTGAAGCGA CAGAAGGACC GAGGGGGAGA CATCTCTCAG AAGACAGTCC TGCCCTTGCA TCTGGTTCAT CATCAGGCAT TGGCAGTGGC AGGCCTGAGC CCCCTCCTCC AGAGAAGCCA TTCCCCTGCC TCATTCCCTA GGCCTTTTGC CACCCCACCA GCCACACCTG GCAGCCGAGG CTGGCCCCCA CAGCCCGTCC CCACCCTGCG GCTTGAGGGG GTCGAGTCCA GTGAGAAACT CAACAGCAGC TTCCCATCCA TCCACTGCGG CTCCTGGGCT GAGACCACCC CCGGTGGCGG GGGCAGCAGC GCCGCCCGGA GAGTCCGGCC CGTCTCCCTC ATGGTGCCCA GCCAGGCTGG GGCCCCAGGG AGGCAGTTCC ACGGCAGTGC CAGCAGCCTG GTGGAAGCGG TCTTGATTTC AGAAGGACTG GGGCAGTTTG CTCAAGATCC CAAGTTCATC GAGGTCACCA CCCAGGAGCT GGCCGACGCC TGCGACATGA CCATAGAGGA GATGGAGAGC GCGGCCGACA ACATCCTCAG CGGGGGCGCC CCACAGAGCC CCAATGGCGC CCTCTTACCC TTTGTGAACT GCAGGGACGC GGGGCAGGAC CGAGCCGGGG GCGAAGAGGA CGCGGGCTGT GTGCGCGCGC GGGGTCGACC GAGTGAGGAG GAGCTCCAGG ACAGCAGGGT CTACGTCAGC AGCCTGTAGG CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGA CGCCACCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC AGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGAATT GACTCAAATG ATGTCAATTA GTCTATCAGA AGCTATCTGG TCTCCCTTCC GGGGGACAAG ACATCCCTGT TTAATATTTA AACAGCAGTG TTCCCAAACT GGGTTCTTAT ATCCCTTGCT CTGGTCAACC AGGTTGCAGG GTTTCCTGTC CTCACAGGAA CGAAGTCCCT AAAGAAACAG TGGCAGCCAG GTTTAGCCCC GGAATTGACT GGATTCCTTT TTTAGGGCCC ATTGGTATGG CTTTTTCCCC GTATCCCCCC AGGTGTCTGC AGGCTCAAAG AGCAGCGAGA AGCGTTCAGA GGAAAGCGAT CCCGTGCCAC CTTCCCCGTG CCCGGGCTGT CCCCGCACGC TGCCGGCTCG GGGATGCGGG GGGAGCGCCG GACCGGAGCG GAGCCCCGGG CGGCTCGCTG CTGCCCCCTA GCGGGGGAGG GACGTAATTA CATCCCTGGG GGCTTTGGGG GGGGGCTGTC CCTGATATCT ATAACAAGAA AATATATATA TAATAAGTTA TCACGTAAGT AGAACATGAA ATAACAATAT AATTATCGTA TGAGTTAAAT CTTAAAAGTC ACGTAAAAGA TAATCATGCG TCATTTTGAC TCACGCGGTC GTTATAGTTC AAAATCAGTG ACACTTACCG CATTGACAAG CACGCCTCAC GGGAGCTCCA AGCGGCGACT GAGATGTCCT AAATGCACAG CGACGGATTC GCGCTATTTA GAAAGAGAGA GCAATATTTC AAGAATGCAT GCGTCAATTT TACGCAGACT ATCTTTCTAG GGTTAATCTA GCTGCATCAG GATCATATCG TCGGGTCTTT TTTCCGGCTC AGTCATCGCC CAAGCTGGCG CTATCTGGGC ATCGGGGAGG AAGAAGCCCG TGCCTTTTCC CGCGAGGTTG AAGCGGCATG GAAAGAGTTT GCCGAGGATG ACTGCTGCTG CATTGACGTT GAGCGAAAAC GCACGTTTAC CATGATGATT CGGGAAGGTG TGGCCATGCA CGCCTTTAAC GGTGAACTGT TCGTTCAGGC CACCTGGGAT ACCAGTTCGT CGCGGCTTTT CCGGACACAG TTCCGGATGG TCAGCCCGAA GCGCATCAGC AACCCGAACA ATACCGGCGA CAGCCGGAAC TGCCGTGCCG GTGTGCAGAT TAATGACAGC GGTGCGGCGC TGGGATATTA CGTCAGCGAG GACGGGTATC CTGGCTGGAT GCCGCAGAAA TGGACATGGA TACCCCGTGA GTTACCCGGC GGGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCAT
