pPIC9k-CBDAS(Cannabis)-6×His
$84.00
In stock
SKU
PL56750
100μg liquid plasmid(Instantly):$105
| name | pPIC9k-CBDAS(Cannabis)-6×His | another name | apPIC9k-CBDAS(Cannabis)-6×His |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Streptomyces | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418,HIS4 | Fluorescent labeling | |
| Promoter | AOX1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAAACGAT GAGATTTCCT TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG TTTATAATGA AGTGCTCAAC ATTCTCCTTT TGGTTTGTTT GCAAGATAAT ATTTTTCTTT TTCTCATTCA ATATCCAAAC TTCCATTGCT AATCCTCGAG AAAACTTCCT TAAATGCTTC TCGCAATATA TTCCCAATAA TGCAACAAAT CTAAAACTCG TATACACTCA AAACAACCCA TTGTATATGT CTGTCCTAAA TTCGACAATA CACAATCTTA GATTCACCTC TGACACAACC CCAAAACCAC TTGTTATCGT CACTCCTTCA CATGTCTCTC ATATCCAAGG CACTATTCTA TGCTCCAAGA AAGTTGGCTT GCAGATTCGA ACTCGAAGTG GTGGTCATGA TTCTGAGGGC ATGTCCTACA TATCTCAAGT CCCATTTGTT ATAGTAGACT TGAGAAACAT GCGTTCAATC AAAATAGATG TTCATAGCCA AACTGCATGG GTTGAAGCCG GAGCTACCCT TGGAGAAGTT TATTATTGGG TTAATGAGAA AAATGAGAAT CTTAGTTTGG CGGCTGGGTA TTGCCCTACT GTTTGCGCAG GTGGACACTT TGGTGGAGGA GGCTATGGAC CATTGATGAG AAACTATGGC CTCGCGGCTG ATAATATCAT TGATGCACAC TTAGTCAACG TTCATGGAAA AGTGCTAGAT CGAAAATCTA TGGGGGAAGA TCTCTTTTGG GCTTTACGTG GTGGTGGAGC AGAAAGCTTC GGAATCATTG TAGCATGGAA AATTAGACTG GTTGCTGTCC CAAAGTCTAC TATGTTTAGT GTTAAAAAGA TCATGGAGAT ACATGAGCTT GTCAAGTTAG TTAACAAATG GCAAAATATT GCTTACAAGT ATGACAAAGA TTTATTACTC ATGACTCACT TCATAACTAG GAACATTACA GATAATCAAG GGAAGAATAA GACAGCAATA CACACTTACT TCTCTTCAGT TTTCCTTGGT GGAGTGGATA GTCTAGTCGA CTTGATGAAC AAGAGTTTTC CTGAGTTGGG TATTAAAAAA ACGGATTGCA GACAATTGAG CTGGATTGAT ACTATCATCT TCTATAGTGG TGTTGTAAAT TACGACACTG ATAATTTTAA CAAGGAAATT TTGCTTGATA GATCCGCTGG GCAGAACGGT GCTTTCAAGA TTAAGTTAGA CTACGTTAAG AAACCAATTC CAGAATCTGT ATTTGTCCAA ATTTTGGAAA AATTATATGA AGAAGATATA GGAGCTGGGA TGTATGCGTT GTACCCTTAC GGTGGTATAA TGGATGAGAT TTCAGAATCA GCAATTCCAT TCCCTCATCG AGCTGGAATC TTGTATGAGT TATGGTACAT ATGTAGTTGG GAGAAGCAAG AAGATAACGA AAAGCATCTA AACTGGATTA GAAATATTTA TAACTTCATG ACTCCTTATG TGTCCAAAAA TCCAAGATTG GCATATCTCA ATTATAGAGA CCTTGATATA GGAATAAATG ATCCCAAGAA TCCAAATAAT TACACACAAG CACGTATTTG GGGTGAGAAG TATTTTGGTA AAAATTTTGA CAGGCTAGTA AAAGTGAAAA CCCTGGTTGA TCCCAATAAC TTTTTTAGAA ACGAACAAAG CATCCCACCT CTTCCACGGC ATCGTCATGA ATTCCCTAGG GCGGCCGCGA ATCATCATCA CCATCACCAT TAATTCGCCT TAGACATGAC TGTTCCTCAG TTCAAGTTGG GCACTTACGA GAAGACCGGT CTTGCTAGAT TCTAATCAAG AGGATGTCAG AATGCCATTT GCCTGAGAGA TGCAGGCTTC ATTTTTGATA CTTTTTTATT TGTAACCTAT ATAGTATAGG ATTTTTTTTG TCATTTTGTT TCTTCTCGTA CGAGCTTGCT CCTGATCAGC CTATCTCGCA GCTGATGAAT ATCTTGTGGT AGGGGTTTGG GAAAATCATT CGAGTTTGAT GTTTTTCTTG GTATTTCCCA CTCCTCTTCA GAGTACAGAA GATTAAGTGA GAAGTTCGTT TGTGCAAGCT TATCGATAAG CTTTAATGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGGCACC GTGTATGAAA TCTAACAATG CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC CGGTACTGCC GGGCCTCTTG CGGGATATCG TCCATTCCGA CAGCATCGCC AGTCACTATG GCGTGCTGCT AGCGCTATAT GCGTTGATGC AATTTCTATG CGCACCCGTT CTCGGAGCAC TGTCCGACCG CTTTGGCCGC CGCCCAGTCC TGCTCGCTTC GCTACTTGGA GCCACTATCG ACTACGCGAT CATGGCGACC ACACCCGTCC TGTGGATCTA TCGAATCTAA ATGTAAGTTA AAATCTCTAA ATAATTAAAT AAGTCCCAGT TTCTCCATAC GAACCTTAAC AGCATTGCGG TGAGCATCTA GACCTTCAAC AGCAGCCAGA TCCATCACTG CTTGGCCAAT ATGTTTCAGT CCCTCAGGAG TTACGTCTTG TGAAGTGATG AACTTCTGGA AGGTTGCAGT GTTAACTCCG CTGTATTGAC GGGCATATCC GTACGTTGGC AAAGTGTGGT TGGTACCGGA GGAGTAATCT CCACAACTCT CTGGAGAGTA GGCACCAACA AACACAGATC CAGCGTGTTG TACTTGATCA ACATAAGAAG AAGCATTCTC GATTTGCAGG ATCAAGTGTT CAGGAGCGTA CTGATTGGAC ATTTCCAAAG CCTGCTCGTA GGTTGCAACC GATAGGGTTG TAGAGTGTGC AATACACTTG CGTACAATTT CAACCCTTGG CAACTGCACA GCTTGGTTGT GAACAGCATC TTCAATTCTG GCAAGCTCCT TGTCTGTCAT ATCGACAGCC AACAGAATCA CCTGGGAATC AATACCATGT TCAGCTTGAG ACAGAAGGTC TGAGGCAACG AAATCTGGAT CAGCGTATTT ATCAGCAATA ACTAGAACTT CAGAAGGCCC AGCAGGCATG TCAATACTAC ACAGGGCTGA TGTGTCATTT TGAACCATCA TCTTGGCAGC AGTAACGAAC TGGTTTCCTG GACCAAATAT TTTGTCACAC TTAGGAACAG TTTCTGTTCC GTAAGCCATA GCAGCTACTG CCTGGGCGCC TCCTGCTAGC ACGATACACT TAGCACCAAC CTTGTGGGCA ACGTAGATGA CTTCTGGGGT AAGGGTACCA TCCTTCTTAG GTGGAGATGC AAAAACAATT TCTTTGCAAC CAGCAACTTT GGCAGGAACA CCCAGCATCA GGGAAGTGGA AGGCAGAATT GCGGTTCCAC CAGGAATATA GAGGCCAACT TTCTCAATAG GTCTTGCAAA ACGAGAGCAG ACTACACCAG GGCAAGTCTC AACTTGCAAC GTCTCCGTTA GTTGAGCTTC ATGGAATTTC CTGACGTTAT CTATAGAGAG ATCAATGGCT CTCTTAACGT TATCTGGCAA TTGCATAAGT TCCTCTGGGA AAGGAGCTTC TAACACAGGT GTCTTCAAAG CGACTCCATC AAACTTGGCA GTTAGTTCTA AAAGGGCTTT GTCACCATTT TGACGAACAT TGTCGACAAT TGGTTTGACT AATTCCATAA TCTGTTCCGT TTTCTGGATA GGACGACGAA GGGCATCTTC AATTTCTTGT GAGGAGGCCT TAGAAACGTC AATTTTGCAC AATTCAATAC GACCTTCAGA AGGGACTTCT TTAGGTTTGG ATTCTTCTTT AGGTTGTTCC TTGGTGTATC CTGGCTTGGC ATCTCCTTTC CTTCTAGTGA CCTTTAGGGA CTTCATATCC AGGTTTCTCT CCACCTCGTC CAACGTCACA CCGTACTTGG CACATCTAAC TAATGCAAAA TAAAATAAGT CAGCACATTC CCAGGCTATA TCTTCCTTGG ATTTAGCTTC TGCAAGTTCA TCAGCTTCCT CCCTAATTTT AGCGTTCAAC AAAACTTCGT CGTCAAATAA CCGTTTGGTA TAAGAACCTT CTGGAGCATT GCTCTTACGA TCCCACAAGG TGGCTTCCAT GGCTCTAAGA CCCTTTGATT GGCCAAAACA GGAAGTGCGT TCCAAGTGAC AGAAACCAAC ACCTGTTTGT TCAACCACAA ATTTCAAGCA GTCTCCATCA CAATCCAATT CGATACCCAG CAACTTTTGA GTTGCTCCAG ATGTAGCACC TTTATACCAC AAACCGTGAC GACGAGATTG GTAGACTCCA GTTTGTGTCC TTATAGCCTC CGGAATAGAC TTTTTGGACG AGTACACCAG GCCCAACGAG TAATTAGAAG AGTCAGCCAC CAAAGTAGTG AATAGACCAT CGGGGCGGTC AGTAGTCAAA GACGCCAACA AAATTTCACT GACAGGGAAC TTTTTGACAT CTTCAGAAAG TTCGTATTCA GTAGTCAATT GCCGAGCATC AATAATGGGG ATTATACCAG AAGCAACAGT GGAAGTCACA TCTACCAACT TTGCGGTCTC AGAAAAAGCA TAAACAGTTC TACTACCGCC ATTAGTGAAA CTTTTCAAAT CGCCCAGTGG AGAAGAAAAA GGCACAGCGA TACTAGCATT AGCGGGCAAG GATGCAACTT TATCAACCAG GGTCCTATAG ATAACCCTAG CGCCTGGGAT CATCCTTTGG ACAACTCTTT CTGCCAAATC TAGGTCCAAA ATCACTTCAT TGATACCATT ATTGTACAAC TTGAGCAAGT TGTCGATCAG CTCCTCAAAT TGGTCCTCTG TAACGGATGA CTCAACTTGC ACATTAACTT GAAGCTCAGT CGATTGAGTG AACTTGATCA GGTTGTGCAG CTGGTCAGCA GCATAGGGAA ACACGGCTTT TCCTACCAAA CTCAAGGAAT TATCAAACTC TGCAACACTT GCGTATGCAG GTAGCAAGGG AAATGTCATA CTTGAAGTCG GACAGTGAGT GTAGTCTTGA GAAATTCTGA AGCCGTATTT TTATTATCAG TGAGTCAGTC ATCAGGAGAT CCTCTACGCC GGACGCATCG TGGCCGACCT GCAGGGGGGG GGGGGGCGCT GAGGTCTGCC TCGTGAAGAA GGTGTTGCTG ACTCATACCA GGCCTGAATC GCCCCATCAT CCAGCCAGAA AGTGAGGGAG CCACGGTTGA TGAGAGCTTT GTTGTAGGTG GACCAGTTGG TGATTTTGAA CTTTTGCTTT GCCACGGAAC GGTCTGCGTT GTCGGGAAGA TGCGTGATCT GATCCTTCAA CTCAGCAAAA GTTCGATTTA TTCAACAAAG CCGCCGTCCC GTCAAGTCAG CGTAATGCTC TGCCAGTGTT ACAACCAATT AACCAATTCT GATTAGAAAA ACTCATCGAG CATCAAATGA AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT TTTGAAAAAG CCGTTTCTGT AATGAAGGAG AAAACTCACC GAGGCAGTTC CATAGGATGG CAAGATCCTG GTATCGGTCT GCGATTCCGA CTCGTCCAAC ATCAATACAA CCTATTAATT TCCCCTCGTC AAAAATAAGG TTATCAAGTG AGAAATCACC ATGAGTGACG ACTGAATCCG GTGAGAATGG CAAAAGCTTA TGCATTTCTT TCCAGACTTG TTCAACAGGC CAGCCATTAC GCTCGTCATC AAAATCACTC GCATCAACCA AACCGTTATT CATTCGTGAT TGCGCCTGAG CGAGACGAAA TACGCGATCG CTGTTAAAAG GACAATTACA AACAGGAATC GAATGCAACC GGCGCAGGAA CACTGCCAGC GCATCAACAA TATTTTCACC TGAATCAGGA TATTCTTCTA ATACCTGGAA TGCTGTTTTC CCGGGGATCG CAGTGGTGAG TAACCATGCA TCATCAGGAG TACGGATAAA ATGCTTGATG GTCGGAAGAG GCATAAATTC CGTCAGCCAG TTTAGTCTGA CCATCTCATC TGTAACATCA TTGGCAACGC TACCTTTGCC ATGTTTCAGA AACAACTCTG GCGCATCGGG CTTCCCATAC AATCGATAGA TTGTCGCACC TGATTGCCCG ACATTATCGC GAGCCCATTT ATACCCATAT AAATCAGCAT CCATGTTGGA ATTTAATCGC GGCCTCGAGC AAGACGTTTC CCGTTGAATA TGGCTCATAA CACCCCTTGT ATTACTGTTT ATGTAAGCAG ACAGTTTTAT TGTTCATGAT GATATATTTT TATCTTGTGC AATGTAACAT CAGAGATTTT GAGACACAAC GTGGCTTTCC CCCCCCCCCC TGCAGGTCGG CATCACCGGC GCCACAGGTG CGGTTGCTGG CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT CGCCACTTCG GGCTCATGAG CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC GGGGGACTGT TGGGCGCCAT CTCCTTGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA GTATCTATGA TTGGAAGTAT GGGAATGGTG ATACCCGCAT TCTTCAGTGT CTTGAGGTCT CCTATCAGAT TATGCCCAAC TAAAGCAACC GGAGGAGGAG ATTTCATGGT AAATTTCTCT GACTTTTGGT CATCAGTAGA CTCGAACTGT GAGACTATCT CGGTTATGAC AGCAGAAATG TCCTTCTTGG AGACAGTAAA TGAAGTCCCA CCAATAAAGA AATCCTTGTT ATCAGGAACA AACTTCTTGT TTCGAACTTT TTCGGTGCCT TGAACTATAA AATGTAGAGT GGATATGTCG GGTAGGAATG GAGCGGGCAA ATGCTTACCT TCTGGACCTT CAAGAGGTAT GTAGGGTTTG TAGATACTGA TGCCAACTTC AGTGACAACG TTGCTATTTC GTTCAAACCA TTCCGAATCC AGAGAAATCA AAGTTGTTTG TCTACTATTG ATCCAAGCCA GTGCGGTCTT GAAACTGACA ATAGTGTGCT CGTGTTTTGA GGTCATCTTT GTATGAATAA ATCTAGTCTT TGATCTAAAT AATCTTGACG AGCCAAGGCG ATAAATACCC AAATCTAAAA CTCTTTTAAA ACGTTAAAAG GACAAGTATG TCTGCCTGTA TTAAACCCCA AATCAGCTCG TAGTCTGATC CTCATCAACT TGAGGGGCAC TATCTTGTTT TAGAGAAATT TGCGGAGATG CGATATCGAG AAAAAGGTAC GCTGATTTTA AACGTGAAAT TTATCTCAAG ATCTCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCAAGAAT TAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTGACT CATGTTGGTA TTGTGAAATA GACGCAGATC GGGAACACTG AAAAATAACA GTTATTATTC G
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