pEnCMV-CREBBP(human)-2-3×FLAG-SV40-Neo(1synonymous mutations)
$157.00
In stock
SKU
PL56591
100μg liquid plasmid(Instantly):$178
| name | pEnCMV-CREBBP(human)-2-3×FLAG-SV40-Neo(1synonymous mutations) | another name | apEnCMV-CREBBP(human)-2-3×FLAG-SV40-Neo(1synonymous mutations) |
| Insert Size (bp) | 7326bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGGCTGAGA ACTTGCTGGA CGGACCGCCC AACCCCAAAA GAGCCAAACT CAGCTCGCCC GGTTTCTCGG CGAATGACAG CACAGATTTT GGATCATTGT TTGACTTGGA AAATGATCTT CCTGATGAGC TGATACCCAA TGGAGGAGAA TTAGGCCTTT TAAACAGTGG GAACCTTGTT CCAGATGCTG CTTCCAAACA TAAACAACTG TCGGAGCTTC TACGAGGAGG CAGCGGCTCT AGTATCAACC CAGGAATAGG AAATGTGAGC GCCAGCAGCC CCGTGCAGCA GGGCCTGGGT GGCCAGGCTC AAGGGCAGCC GAACAGTGCT AACATGGCCA GCCTCAGTGC CATGGGCAAG AGCCCTCTGA GCCAGGGAGA TTCTTCAGCC CCCAGCCTGC CTAAACAGGC AGCCAGCACC TCTGGGCCCA CCCCCGCTGC CTCCCAAGCA CTGAATCCGC AAGCACAAAA GCAAGTGGGG CTGGCGACTA GCAGCCCTGC CACGTCACAG ACTGGACCTG GTATCTGCAT GAATGCTAAC TTTAACCAGA CCCACCCAGG CCTCCTCAAT AGTAACTCTG GCCATAGCTT AATTAATCAG GCTTCACAAG GGCAGGCGCA AGTCATGAAT GGATCTCTTG GGGCTGCTGG CAGAGGAAGG GGAGCTGGAA TGCCGTACCC TACTCCAGCC ATGCAGGGCG CCTCGAGCAG CGTGCTGGCT GAGACCCTAA CGCAGGTTTC CCCGCAAATG ACTGGTCACG CGGGACTGAA CACCGCACAG GCAGGAGGCA TGGCCAAGAT GGGAATAACT GGGAACACAA GTCCATTTGG ACAGCCCTTT AGTCAAGCTG GAGGGCAGCC AATGGGAGCC ACTGGAGTGA ACCCCCAGTT AGCCAGCAAA CAGAGCATGG TCAACAGTTT GCCCACCTTC CCTACAGATA TCAAGAATAC TTCAGTCACC AACGTGCCAA ATATGTCTCA GATGCAAACA TCAGTGGGAA TTGTACCCAC ACAAGCAATT GCAACAGGCC CCACTGCAGA TCCTGAAAAA CGCAAACTGA TACAGCAGCA GCTGGTTCTA CTGCTTCATG CTCATAAGTG TCAGAGACGA GAGCAAGCAA ACGGAGAGGT TCGGGCCTGC TCGCTCCCGC ATTGTCGAAC CATGAAAAAC GTTTTGAATC ACATGACGCA TTGTCAGGCT GGGAAAGCCT GCCAAGTTGC CCATTGTGCA TCTTCACGAC AAATCATCTC TCATTGGAAG AACTGCACAC GACATGACTG TCCTGTTTGC CTCCCTTTGA AAAATGCCAG TGACAAGCGA AACCAACAAA CCATCCTGGG GTCTCCAGCT AGTGGAATTC AAAACACAAT TGGTTCTGTT GGCACAGGGC AACAGAATGC CACTTCTTTA AGTAACCCAA ATCCCATAGA CCCCAGCTCC ATGCAGCGAG CCTATGCTGC TCTCGGACTC CCCTACATGA ACCAGCCCCA GACGCAGCTG CAGCCTCAGG TTCCTGGCCA GCAACCAGCA CAGCCTCAAA CCCACCAGCA GATGAGGACT CTCAACCCCC TGGGAAATAA TCCAATGAAC ATTCCAGCAG GAGGAATAAC AACAGATCAG CAGCCCCCAA ACTTGATTTC AGAATCAGCT CTTCCGACTT CCCTGGGGGC CACAAACCCA CTGATGAACG ATGGCTCCAA CTCTGGTAAC ATTGGAACCC TCAGCACTAT ACCAACAGCA GCTCCTCCTT CTAGCACCGG TGTAAGGAAA GGCTGGCACG AACATGTCAC TCAGGACCTG CGGAGCCATC TAGTGCATAA ACTCGTCCAA GCCATCTTCC CAACACCTGA TCCCGCAGCT CTAAAGGATC GCCGCATGGA AAACCTGGTA GCCTATGCTA AGAAAGTGGA AGGGGACATG TACGAGTCTG CCAACAGCAG GGATGAATAT TATCACTTAT TAGCAGAGAA AATCTACAAG ATACAAAAAG AACTAGAAGA AAAACGGAGG TCGCGTTTAC ATAAACAAGG CATCTTGGGG AACCAGCCAG CCTTACCAGC CCCGGGGGCT CAGCCCCCTG TGATTCCACA GGCACAACCT GTGAGACCTC CAAATGGACC CCTGTCCCTG CCAGTGAATC GCATGCAAGT TTCTCAAGGG ATGAATTCAT TTAACCCCAT GTCCTTGGGG AACGTCCAGT TGCCACAAGC ACCCATGGGA CCTCGTGCAG CCTCCCCAAT GAACCACTCT GTCCAGATGA ACAGCATGGG CTCAGTGCCA GGGATGGCCA TTTCTCCTTC CCGAATGCCT CAGCCTCCGA ACATGATGGG TGCACACACC AACAACATGA TGGCCCAGGC GCCCGCTCAG AGCCAGTTTC TGCCACAGAA CCAGTTCCCG TCATCCAGCG GGGCGATGAG TGTGGGCATG GGGCAGCCGC CAGCCCAAAC AGGCGTGTCA CAGGGACAGG TGCCTGGTGC TGCTCTTCCT AACCCTCTCA ACATGCTGGG GCCTCAGGCC AGCCAGCTAC CTTGCCCTCC AGTGACACAG TCACCACTGC ACCCAACACC GCCTCCTGCT TCCACGGCTG CTGGCATGCC ATCTCTCCAG CACACGACAC CACCTGGGAT GACTCCTCCC CAGCCAGCAG CTCCCACTCA GCCATCAACT CCTGTGTCGT CTTCCGGGCA GACTCCCACC CCGACTCCTG GCTCAGTGCC CAGTGCTACC CAAACCCAGA GCACCCCTAC AGTCCAGGCA GCAGCCCAGG CCCAGGTGAC CCCGCAGCCT CAAACCCCAG TTCAGCCCCC GTCTGTGGCT ACCCCTCAGT CATCGCAGCA ACAGCCGACG CCTGTGCACG CCCAGCCTCC TGGCACACCG CTTTCCCAGG CAGCAGCCAG CATTGATAAC AGAGTCCCTA CCCCCTCCTC GGTGGCCAGC GCAGAAACCA ATTCCCAGCA GCCAGGACCT GACGTACCTG TGCTGGAAAT GAAGACGGAG ACCCAAGCAG AGGACACTGA GCCCGATCCT GGTGAATCCA AAGGGGAGCC CAGGTCTGAG ATGATGGAGG AGGATTTGCA AGGAGCTTCC CAAGTTAAAG AAGAAACAGA CATAGCAGAG CAGAAATCAG AACCAATGGA AGTGGATGAA AAGAAACCTG AAGTGAAAGT AGAAGTTAAA GAGGAAGAAG AGAGTAGCAG TAACGGCACA GCCTCTCAGT CAACATCTCC TTCGCAGCCG CGCAAAAAAA TCTTTAAACC AGAGGAGTTA CGCCAGGCCC TCATGCCAAC CCTAGAAGCA CTGTATCGAC AGGACCCAGA GTCATTACCT TTCCGGCAGC CTGTAGATCC CCAGCTCCTC GGAATTCCAG ACTATTTTGA CATCGTAAAG AATCCCATGG ACCTCTCCAC CATCAAGCGG AAGCTGGACA CAGGGCAATA CCAAGAGCCC TGGCAGTACG TGGACGACGT CTGGCTCATG TTCAACAATG CCTGGCTCTA TAATCGCAAG ACATCCCGAG TCTATAAGTT TTGCAGTAAG CTTGCAGAGG TCTTTGAGCA GGAAATTGAC CCTGTCATGC AGTCCCTTGG ATATTGCTGT GGACGCAAGT ATGAGTTTTC CCCACAGACT TTGTGCTGCT ATGGGAAGCA GCTGTGTACC ATTCCTCGCG ATGCTGCCTA CTACAGCTAT CAGAATAGGT ATCATTTCTG TGAGAAGTGT TTCACAGAGA TCCAGGGCGA GAATGTGACC CTGGGTGACG ACCCTTCACA GCCCCAGACG ACAATTTCAA AGGATCAGTT TGAAAAGAAG AAAAATGATA CCTTAGACCC CGAACCTTTC GTTGATTGCA AGGAGTGTGG CCGGAAGATG CATCAGATTT GCGTTCTGCA CTATGACATC ATTTGGCCTT CAGGTTTTGT GTGCGACAAC TGCTTGAAGA AAACTGGCAG ACCTCGAAAA GAAAACAAAT TCAGTGCTAA GAGGCTGCAG ACCACAAGAC TGGGAAACCA CTTGGAAGAC CGAGTGAACA AATTTTTGCG GCGCCAGAAT CACCCTGAAG CCGGGGAGGT TTTTGTCCGA GTGGTGGCCA GCTCAGACAA GACGGTGGAG GTCAAGCCCG GGATGAAGTC ACGGTTTGTG GATTCTGGGG AAATGTCTGA ATCTTTCCCA TATCGAACCA AAGCTCTGTT TGCTTTTGAG GAAATTGACG GCGTGGATGT CTGCTTTTTT GGAATGCACG TCCAAGAATA CGGCTCTGAT TGCCCCCCTC CAAACACGAG GCGTGTGTAC ATTTCTTATC TGGATAGTAT TCATTTCTTC CGGCCACGTT GCCTCCGCAC AGCCGTTTAC CATGAGATCC TTATTGGATA TTTAGAGTAT GTGAAGAAAT TAGGGTATGT GACAGGGCAC ATCTGGGCCT GTCCTCCAAG TGAAGGAGAT GATTACATCT TCCATTGCCA CCCACCTGAT CAAAAAATAC CCAAGCCAAA ACGACTGCAG GAGTGGTACA AAAAGATGCT GGACAAGGCG TTTGCAGAGC GGATCATCCA TGACTACAAG GATATTTTCA AACAAGCAAC TGAAGACAGG CTCACCAGTG CCAAGGAACT GCCCTATTTT GAAGGTGATT TCTGGCCCAA TGTGTTAGAA GAGAGCATTA AGGAACTAGA ACAAGAAGAA GAGGAGAGGA AAAAGGAAGA GAGCACTGCA GCCAGTGAAA CCACTGAGGG CAGTCAGGGC GACAGCAAGA ATGCCAAGAA GAAGAACAAC AAGAAAACCA ACAAGAACAA AAGCAGCATC AGCCGCGCCA ACAAGAAGAA GCCCAGCATG CCCAACGTGT CCAATGACCT GTCCCAGAAG CTGTATGCCA CCATGGAGAA GCACAAGGAG GTCTTCTTCG TGATCCACCT GCACGCTGGG CCTGTCATCA ACACCCTGCC CCCCATCGTC GACCCCGACC CCCTGCTCAG CTGTGACCTC ATGGATGGGC GCGACGCCTT CCTCACCCTC GCCAGAGACA AGCACTGGGA GTTCTCCTCC TTGCGCCGCT CCAAGTGGTC CACGCTCTGC ATGCTGGTGG AGCTGCACAC CCAGGGCCAG GACCGCTTTG TCTACACCTG CAACGAGTGC AAGCACCACG TGGAGACGCG CTGGCACTGC ACTGTGTGCG AGGACTACGA CCTCTGCATC AACTGCTATA ACACGAAGAG CCATGCCCAT AAGATGGTGA AGTGGGGGCT GGGCCTGGAT GACGAGGGCA GCAGCCAGGG CGAGCCACAG TCAAAGAGCC CCCAGGAGTC ACGCCGGCTG AGCATCCAGC GCTGCATCCA GTCGCTGGTG CACGCGTGCC AGTGCCGCAA CGCCAACTGC TCGCTGCCAT CCTGCCAGAA GATGAAGCGG GTGGTGCAGC ACACCAAGGG CTGCAAACGC AAGACCAACG GGGGCTGCCC GGTGTGCAAG CAGCTCATCG CCCTCTGCTG CTACCACGCC AAGCACTGCC AAGAAAACAA ATGCCCCGTG CCCTTCTGCC TCAACATCAA ACACAAGCTC CGCCAGCAGC AGATCCAGCA CCGCCTGCAG CAGGCCCAGC TCATGCGCCG GCGGATGGCC ACCATGAACA CCCGCAACGT GCCTCAGCAG AGTCTGCCTT CTCCTACCTC AGCACCGCCC GGGACCCCCA CACAGCAGCC CAGCACACCC CAGACGCCGC AGCCCCCTGC CCAGCCCCAA CCCTCACCCG TGAGCATGTC ACCAGCTGGC TTCCCCAGCG TGGCCCGGAC TCAGCCCCCC ACCACGGTGT CCACAGGGAA GCCTACCAGC CAGGTGCCGG CCCCCCCACC CCCGGCCCAG CCCCCTCCTG CAGCGGTGGA AGCGGCTCGG CAGATCGAGC GTGAGGCCCA GCAGCAGCAG CACCTGTACC GGGTGAACAT CAACAACAGC ATGCCCCCAG GACGCACGGG CATGGGGACC CCGGGGAGCC AGATGGCCCC CGTGAGCCTG AATGTGCCCC GACCCAACCA GGTGAGCGGG CCCGTCATGC CCAGCATGCC TCCCGGGCAG TGGCAGCAGG CGCCCCTTCC CCAGCAGCAG CCCATGCCAG GCTTGCCCAG GCCTGTGATA TCCATGCAGG CCCAGGCGGC CGTGGCTGGG CCCCGGATGC CCAGCGTGCA GCCACCCAGG AGCATCTCAC CCAGCGCTCT GCAAGACCTG CTGCGGACCC TGAAGTCGCC CAGCTCCCCT CAGCAGCAAC AGCAGGTGCT GAACATTCTC AAATCAAACC CGCAGCTAAT GGCAGCTTTC ATCAAACAGC GCACAGCCAA GTACGTGGCC AATCAGCCCG GCATGCAGCC CCAGCCTGGC CTCCAGTCCC AGCCCGGCAT GCAACCCCAG CCTGGCATGC ACCAGCAGCC CAGCCTGCAG AACCTGAATG CCATGCAGGC TGGCGTGCCG CGGCCCGGTG TGCCTCCACA GCAGCAGGCG ATGGGAGGCC TGAACCCCCA GGGCCAGGCC TTGAACATCA TGAACCCAGG ACACAACCCC AACATGGCGA GTATGAATCC ACAGTACCGA GAAATGTTAC GGAGGCAGCT GCTGCAGCAG CAGCAGCAAC AGCAGCAGCA ACAACAGCAG CAACAGCAGC AGCAGCAAGG GAGTGCCGGC ATGGCTGGGG GCATGGCGGG GCACGGCCAG TTCCAGCAGC CTCAAGGACC TGGAGGCTAC CCACCGGCCA TGCAGCAGCA GCAGCGCATG CAGCAGCATC TCCCCCTCCA GGGCAGCTCC ATGGGCCAGA TGGCGGCTCA GATGGGACAG CTTGGCCAGA TGGGGCAGCC GGGGCTGGGG GCAGACAGCA CCCCCAACAT CCAGCAAGCC CTGCAGCAGC GGATTCTGCA GCAACAGCAG ATGAAGCAGC AGATTGGGTC CCCAGGCCAG CCGAACCCCA TGAGCCCCCA GCAACACATG CTCTCAGGAC AGCCACAGGC CTCGCATCTC CCTGGCCAGC AGATCGCCAC GTCCCTTAGT AACCAGGTGC GGTCTCCAGC CCCTGTCCAG TCTCCACGGC CCCAGTCCCA GCCTCCACAT TCCAGCCCGT CACCACGGAT ACAGCCCCAG CCTTCGCCAC ACCACGTCTC ACCCCAGACT GGTTCCCCCC ACCCCGGACT CGCAGTCACC ATGGCCAGCT CCATAGATCA GGGACACTTG GGGAACCCCG AACAGAGTGC AATGCTCCCC CAGCTGAACA CCCCCAGCAG GAGTGCGCTG TCCAGCGAAC TGTCCCTGGT CGGGGACACC ACGGGGGACA CGCTAGAGAA GTTTGTGGAG GGCTTGGAAT TCTGCAGATA TCCAGCACAG TGGCGGCCGC TCGAGGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC
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