pNZ8148-Pnis-MCS1-P32-MCS2
$142.00
In stock
SKU
PL56561
100μg liquid plasmid(Instantly):$163
| name | pNZ8148-Pnis-MCS1-P32-MCS2 | another name | apNZ8148-Pnis-MCS1-P32-MCS2 |
| Insert Size (bp) | Plasmid host | lactic acid bacteria | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Chl | Fluorescent labeling | |
| Promoter | PnisA,P32 | Replicon | repA |
| Competence | MC1061 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAGTC TTATAACTAT ACTGACAATA GAAACATTAA CAAATCTAAA ACAGTCTTAA TTCTATCTTG AGAAAGTATT GGTAATAATA TTATTGTCGA TAACGCGAGC ATAATAAACG GCTCTGATTA AATTCTGAAG TTTGTTAGAT ACAATGATTT CGTTCGAAGG AACTACAAAA TAAATTATAA GGAGGCACTC ACCATGGGTA CTGCAGGCAT GCGGTACCAC TAGTTCTAGA GAGCTCAAGC TTTTCAAGGC AATCTGCCTC CTCATCCTCT TCATCCTCTT CGTCTTGGTA GCTTTTTAAA TATGGGTCGA TCGAATTCGG TCCTCGGGAT ATGATAAGAT TAATAGTTTT AGCTATTAAT CTTTTTTTAT TTTTATTTAA GAATGGCTTA ATAAAGCGGT TACTTTGGAT TTTTGTGAGC TTGGACTAGA AAAAAACTTC ACAAAATGCT ATACTAGGTA GGTAAAAAAA TATTCGGAGG AATTTTGAAT CTTTGAACCA AAGGATCCAT TAGACCAAGG CCTCGAGTTG AAACGTTCAA TTGAAATAAG CTTGGCAATA TTACAGCACG TGTTGCTTTG ATTGATAGCC AAAAAGCAGC AGTTGATAAA GCAATTACTG ATATTGCTGA AAAATTGTAA TTTATAAATA AAAATCACCT TTTAGAGGTG GTTTTTTTAT TTATAAATTA TTCGTTTGAT TTCGCTTTCG ATAGAACAAT CAAATCGTTT CTGAGACGTT TTAGCGTTTA TTTCGTTTAG TTATCGGCAT AATCGTTAAA ACAGGCGTTA TCGTAGCGTA AAAGCCCTTG AGCGTAGCGT GGCTTTGCAG CGAAGATGTT GTCTGTTAGA TTATGAAAGC CGATGACTGA ATGAAATAAT AAGCGCAGCG TCCTTCTATT TCGGTTGGAG GAGGCTCAAG GGAGTTTGAG GGAATGAAAT TCCCTCATGG GTTTGATTTT AAAAATTGCT TGCAATTTTG CCGAGCGGTA GCGCTGGAAA ATTTTTGAAA AAAATTTGGA ATTTGGAAAA AAATGGGGGG AAAGGAAGCG AATTTTGCTT CCGTACTACG ACCCCCCATT AAGTGCCGAG TGCCAATTTT TGTGCCAAAA ACGCTCTATC CCAACTGGCT CAAGGGTTTG AGGGGTTTTT CAATCGCCAA CGAATCGCCA ACGTTTTCGC CAACGTTTTT TATAAATCTA TATTTAAGTA GCTTTATTTT TGTTTTTATG ATTACAAAGT GATACACTAA TTTTATAAAA TTATTTGATT GGAGTTTTTT AAATGGTGAT TTCAGAATCG AAAAAAAGAG TTATGATTTC TCTGACAAAA GAGCAAGATA AAAAATTAAC AGATATGGCG AAACAAAAAG ATTTTTCAAA ATCTGCGGTT GCGGCGTTAG CTATAGAAGA ATATGCAAGA AAGGAATCAG AACAAAAAAA ATAAGCGAAA GCTCGCGTTT TTAGAAGGAT ACGAGTTTTC GCTACTTGTT TTTGATAAGG TAATTATATC ATGGCTATTA AAAATACTAA AGCTAGAAAT TTTGGATTTT TATTATATCC TGACTCAATT CCTAATGATT GGAAAGAAAA ATTAGAGAGT TTGGGCGTAT CTATGGCTGT CAGTCCTTTA CACGATATGG ACGAAAAAAA AGATAAAGAT ACATGGAATA GTAGTGATGT TATACGAAAT GGAAAGCACT ATAAAAAACC ACACTATCAC GTTATATATA TTGCACGAAA TCCTGTAACA ATAGAAAGCG TTAGGAACAA GATTAAGCGA AAATTGGGGA ATAGTTCAGT TGCTCATGTT GAGATACTTG ATTATATCAA AGGTTCATAT GAATATTTGA CTCATGAATC AAAGGACGCT ATTGCTAAGA ATAAACATAT ATACGACAAA AAAGATATTT TGAACATTAA TGATTTTGAT ATTGACCGCT ATATAACACT TGATGAAAGC CAAAAAAGAG AATTGAAGAA TTTACTTTTA GATATAGTGG ATGACTATAA TTTGGTAAAT ACAAAAGATT TAATGGCTTT TATTCGCCTT AGGGGAGCGG AGTTTGGAAT TTTAAATACG AATGATGTAA AAGATATTGT TTCAACAAAC TCTAGCGCCT TTAGATTATG GTTTGAGGGC AATTATCAGT GTGGATATAG AGCAAGTTAT GCAAAGGTTC TTGATGCTGA AACGGGGGAA ATAAAATGAC AAACAAAGAA AAAGAGTTAT TTGCTGAAAA TGAGGAATTA AAAAAAGAAA TTAAGGACTT AAAAGAGCGT ATTGAAAGAT ACAGAGAAAT GGAAGTTGAA TTAAGTACAA CAATAGATTT ATTGAGAGGA GGGATTATTG AATAAATAAA AGCCCCCCTG ACGAAAGTCG ACGGCAATAG TTACCCTTAT TATCAAGATA AGAAAGAAAA GGATTTTTCG CTACGCTCAA ATCCTTTAAA AAAACACAAA AGACCACATT TTTTAATGTG GTCTTTTATT CTTCAACTAA AGCACCCATT AGTTCAACAA ACGAAAATTG GATAAAGTGG GATATTTTTA AAATATATAT TTATGTTACA GTAATATTGA CTTTTAAAAA AGGATTGATT CTAATGAAGA AAGCAGACAA GTAAGCCTCC TAAATTCACT TTAGATAAAA ATTTAGGAGG CATATCAAAT GAACTTTAAT AAAATTGATT TAGACAATTG GAAGAGAAAA GAGATATTTA ATCATTATTT GAACCAACAA ACGACTTTTA GTATAACCAC AGAAATTGAT ATTAGTGTTT TATACCGAAA CATAAAACAA GAAGGATATA AATTTTACCC TGCATTTATT TTCTTAGTGA CAAGGGTGAT AAACTCAAAT ACAGCTTTTA GAACTGGTTA CAATAGCGAC GGAGAGTTAG GTTATTGGGA TAAGTTAGAG CCACTTTATA CAATTTTTGA TGGTGTATCT AAAACATTCT CTGGTATTTG GACTCCTGTA AAGAATGACT TCAAAGAGTT TTATGATTTA TACCTTTCTG ATGTAGAGAA ATATAATGGT TCGGGGAAAT TGTTTCCCAA AACACCTATA CCTGAAAATG CTTTTTCTCT TTCTATTATT CCTTGGACTT CATTTACTGG GTTTAACTTA AATATCAATA ATAATAGTAA TTACCTTCTA CCCATTATTA CAGCAGGAAA ATTCATTAAT AAAGGTAATT CAATATATTT ACCGCTATCT TTACAGGTAC ATCATTCTGT TTGTGATGGT TATCATGCTG GATTGTTTAT GAACTCTATT CAGGAATTGT CAGATAGGCC TAATGACTGG CTTTTATAAT ATGAGATAAT GCCGACTGTA CTTTTTACAG TCGGTTTTCT AATGTCACTA ACCTGCCCCG TTAGTTGAAG AAGGTTTTTA TATTACAGCT CC
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