pAAV-SMC1A(rat)-FLAG
$113.00
In stock
SKU
PL56551
NM_031683.2;SB1.8; SMC-1A; Smc1l1
100μg liquid plasmid(Instantly):$134
| name | pAAV-SMC1A(rat)-FLAG | another name | apAAV-SMC1A(rat)-FLAG |
| Insert Size (bp) | 3699bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTGGAGC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGTCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGATTC GAATCCCGGC CGGGAACGGT GCATTGGAAC GCGGATTCCC CGTGCCAAGA GTGACGTAAG TACCGCCTAT AGAGTCTATA GGCCCACAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA TTTCTAATAC TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT GTATCATGCC TCTTTGCACC ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA TAGCAATATT TCTGCATATA AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC ACAGCTCCTG GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGAATTGGG ATTCGAACAT CGATTGAATT CCCCGGGGAT CCATGGGTTT CCTGAAACTG ATTGAGATTG AGAACTTTAA ATCGTACAAG GGTCGACAAA TTATCGGACC ATTTCAGAGG TTCACCGCCA TCATTGGACC CAATGGCTCT GGTAAGTCAA ATCTCATGGA TGCCATCAGC TTTGTGCTTG GTGAAAAAAC CAGCAACCTG AGGGTAAAGA CCCTGAGGGA CCTGATACAC GGAGCTCCTG TGGGCAAGCC GGCTGCCAAC CGAGCCTTTG TCAGCATGGT CTACTCCGAG GAGGGTGCTG AGGACCGCAC CTTTGCCCGA GTCATTGTAG GGGGCTCTTC TGAGTACAAG ATCAATAACA AAGTTGTCCA GCTACATGAA TACAGTGAAG AGTTAGAGAA GTTGGGCATT CTCATCAAAG CTCGTAACTT CCTCGTCTTC CAGGGTGCAG TGGAATCTAT TGCCATGAAG AACCCCAAGG AGAGGACAGC TTTATTTGAA GAAATTAGTC GCTCTGGGGA ACTTGCACAG GAGTATGACA AGCGAAAGAA AGAAATGGTG AAGGCTGAAG AAGACACACA GTTTAATTAT CATCGCAAGA AAAATATTGC AGCTGAACGC AAGGAGGCCA AACAAGAAAA GGAAGAGGCT GACCGCTACC AGCGCCTGAA GGATGAGGTA GTACGCGCAC AGGTACAATT GCAGCTTTTT AAACTCTACC ACAACGAAGT GGAGATTGAG AAGCTCAACA AGGAACTGGC CTCGAAGAAC AAGGAAATTG AGAAGGACAA AAAGCGGATG GACAAGGTAG AGGATGAACT GAAGGAGAAG AAGAAAGAGC TGGGCAAAAT GATGCGGGAA CAGCAGCAGA TTGAGAAGGA GATCAAGGAA AAGGACTCAG AATTGAATCA GAAGAGGCCT CAGTACATCA AAGCAAAAGA GAATACATCC CACAAAATCA AGAAGCTTGA AGCAGCCAAG AAATCTCTGC AGAATGCTCA GAAACATTAT AAGAAACGTA AAGGTGACAT GGATGAGCTG GAGAAAGAGA TGCTGTCAGT GGAGAAGGCC CGACAGGAGT TTGAAGAGCG GATGGAGGAA GAGAGTCAGA GTCAAGGCAG AGACTTGACC CTGGAGGAGA ACCAGGTAAA GAAATACCAC CGATTAAAAG AGGAAGCTAG CAAGAGAGCA GCCACTTTGG CTCAGGAGCT GGAGAAGTTT AATCGAGACC AGAAAGCTGA CCAGGACCGT CTAGATCTAG AAGAGCGGAA GAAAGTAGAG ACAGAGGCCA AGATCAAGCA AAAACTGCGG GAGATTGAAG AGAATCAGAA ACGGATTGAG AAGCTAGAGG AATATATTAC CACTAGCAAG CAGTCTCTAG AGGAACAGAA GAAGCTAGAG GGAGAATTGA CAGAAGAAGT AGAGATGGCC AAGCGTCGTA TTGATGAGAT CAATAAGGAG CTGAACCAGG TGATGGAGCA ATTGGGGGAT GCGCGCATTG ACCGGCAGGA GAGCAGCCGC CAGCAGAGGA AGGCAGAAAT AATGGAAAGC ATCAAGCGTC TCTACCCTGG TTCTGTGTAT GGCCGCCTCA TTGACCTCTG CCAGCCTACA CAAAAGAAGT ATCAGATTGC TGTGACCAAG GTTTTAGGAA AGAACATGGA TGCTATTATT GTAGACTCCG AGAAGACAGG TCGGGATTGT ATTCAGTATA TCAAGGAGCA ACGTGGAGAG CCTGAGACCT TCTTGCCTCT TGACTACCTG GAGGTGAAAC CTACTGATGA GAAACTCCGA GAACTGAAGG GGGCCAAGCT AGTGATTGAT GTGATTCGCT ATGAGCCACC TCACATCAAA AAAGCTCTGC AGTATGCTTG TGGCAATGCC CTTGTTTGTG ACAATGTGGA GGATGCGCGA CGAATTGCCT TTGGAGGTCA CCAACGCCAC AAGACAGTGG CATTGGATGG CACCCTGTTC CAGAAGTCAG GAGTTATCTC TGGTGGAGCC AGTGATCTCA AGGCAAAGGC ACGACGCTGG GATGAGAAGG CAGTAGACAA ACTAAAAGAA AAAAAAGAAC GTTTGACGGA GGAGCTAAAA GAGCAGATGA AGGCAAAACG AAAAGAGGCA GAGCTGCGTC AGGTGCAGTC TCAGGCCCAT GGGCTACAAA TGCGGCTGAA GTACTCACAG AGTGACCTAG AACAGACTAA GACAAGGCAT CTTGCCCTGA ATCTCCAGGA AAAATCTAAG CTGGAGAGTG AACTAGCCAA CTTCGGGCCT CGAATTAATG ATATCAAGAG AATCATACAG AGCCGAGAGA GGGAAATGAA AGACTTGAAG GAGAAGATGA ACCAGGTGGA AGATGAGGTG TTTGAAGAGT TTTGTCGGGA GATTGGTGTG CGTAACATCC GCGAATTTGA GGAAGAAAAG GTGAAGCGGC AGAATGAAAT TGCCAAGAAA CGCTTGGAAT TTGAGAATCA GAAGACTCGC TTGGGCATCC AGTTGGATTT TGAAAAGAAT CAGTTGAAGG AGGACCAGGA TAAAGTACAC ATGTGGGAGC AGACAGTAAA AAAGGATGAA AATGAGATAG AAAAGCTTAA GAAGGAGGAG CAAAGACACA TGAAGATAAT AGATGAAACC ATGGCTCAGC TCCAAGACCT TAAGAATCAG CACCTGGCCA AAAAGTCAGA AGTGAATGAC AAGAATCATG AGATGGAAGA AATTCGCAAG AAGCTTGGGG GTGCCAATAA GGAAATGACT CACTTACAGA AGGAAGTGAC AGCCATTGAG ACCAAGCTTG AACAGAAGCG CAGTGACCGC CACAACCTGC TACAGGCCTG TAAGATGCAG GACATCAAAT TGCCACTGTC AAAGGGCACC ATGGATGATA TTAGTCAGGA AGAAGGTGGC TCCCAGGGAG AAGAGTCAGT GAGTGGTTCC CAGAGAACAT CTAGTATCTA TGCACGAGAG GCCCTCATTG AGATTGACTA CGGTGACCTG TGTGAAGATC TGAAGGATGC ACAGGCGGAA GAAGAAATCA AGCAAGAGAT GAACACACTC CAGCAAAAGC TGAATGAGCA GCAGAGTGTG CTTCAGAGGA TTGCTGCCCC TAACATGAAG GCCATGGAAA AGCTGGAAAG TGTCCGAGAC AAGTTCCAGG AGACCTCAGA TGAATTTGAG GCAGCACGAA AGCGAGCAAA GAAAGCCAAG CAGGCATTTG AACAGATAAA GAAGGAGCGC TTTGACCGAT TCAATGCTTG TTTTGAATCT GTGGCTACCA ATATTGATGA GATCTACAAG GCTCTGTCCC GTAACAGCAG TGCCCAGGCA TTCTTGGGCC CCGAGAATCC TGAAGAGCCC TATTTGGATG GTATCAACTA CAACTGTGTA GCTCCTGGCA AACGTTTCCG GCCTATGGAC AATTTGTCAG GCGGGGAGAA GACAGTTGCC GCACTGGCCT TGCTTTTTGC CATTCACAGC TACAAGCCAG CCCCCTTCTT CGTCCTGGAT GAGATTGATG CTGCCTTGGA TAACACCAAC ATTGGCAAGG TGGCAAATTA CATCAAGGAG CAGTCAACTT GCAACTTCCA GGCCATTGTT ATCTCTCTCA AGGAGGAATT CTACACCAAG GCTGAGAGCC TCATTGGAGT CTATCCTGAG CAAGGAGACT GTGTGATCAG CAAAGTCCTG ACCTTCGACC TCACCAAGTA TCCAGATGCC AACCCTAACC CCAATGAACA AGATTACAAG GACGACGATG ACAAGTAAGC TTGCCTCGAG CAGCGCTGCT CGAGAGATCT ACGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTGTA GGTAACCACG TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGT
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