pBiT1.1-N-TK-FLAG-HNRNPL
$84.00
In stock
SKU
PL56529
NM_001533.2;hnRNP-L;HNRPL;P/OKcl.14
100μg liquid plasmid(Instantly):$105
| name | pBiT1.1-N-TK-FLAG-HNRNPL | another name | apBiT1.1-N-TK-FLAG-HNRNPL |
| Insert Size (bp) | 1770bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | TK | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCT GAGTCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA AGTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAA AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAT TACAAGGACG ACGATGACAA GATCTCGAGC ATGTCGCGGA GGCTGCTGCC CCGGGCGGAG AAGCGGCGTC GGCGGCTGGA GCAGAGGCAG CAGCCGGACG AGCAGCGGAG GCGGTCGGGA GCGATGGTGA AGATGGCGGC GGCGGGCGGC GGAGGCGGCG GTGGCCGCTA CTACGGCGGC GGCAGTGAGG GCGGCCGGGC CCCTAAGCGG CTCAAGACTG ACAACGCCGG CGACCAGCAC GGAGGCGGCG GCGGTGGCGG TGGAGGAGCC GGGGCGGCGG GCGGCGGCGG CGGTGGGGAG AACTACGATG ACCCGCACAA AACCCCTGCC TCCCCAGTTG TCCACATCAG GGGCCTGATT GACGGTGTGG TGGAAGCAGA CCTTGTGGAG GCCTTGCAGG AGTTTGGACC CATCAGCTAT GTGGTGGTAA TGCCTAAAAA GAGACAAGCA CTGGTGGAGT TTGAAGATGT GTTGGGGGCT TGCAACGCAG TGAACTACGC AGCCGACAAC CAAATATACA TTGCTGGTCA CCCAGCTTTT GTCAACTACT CTACCAGCCA GAAGATCTCC CGCCCTGGGG ACTCGGATGA CTCCCGGAGC GTGAACAGTG TGCTTCTCTT TACCATCCTG AACCCCATTT ATTCGATCAC CACGGATGTT CTTTACACTA TCTGTAATCC TTGTGGCCCT GTCCAGAGAA TTGTCATTTT CAGGAAGAAT GGAGTTCAGG CGATGGTGGA ATTTGACTCA GTTCAAAGTG CCCAGCGGGC CAAGGCCTCT CTCAATGGGG CTGATATCTA TTCTGGCTGT TGCACTTTGA AGATCGAATA CGCAAAGCCT ACACGCTTGA ATGTGTTCAA GAATGATCAG GATACTTGGG ACTACACAAA CCCCAATCTC AGTGGACAAG GTGACCCTGG CAGCAACCCC AACAAACGCC AGAGGCAGCC CCCTCTCCTG GGAGATCACC CCGCAGAATA TGGAGGGCCC CACGGTGGGT ACCACAGCCA TTACCATGAT GAGGGCTACG GGCCCCCCCC ACCTCACTAC GAAGGGAGAA GGATGGGTCC ACCAGTGGGG GGTCACCGTC GGGGCCCAAG TCGCTACGGC CCCCAGTATG GGCACCCCCC ACCCCCTCCC CCACCACCCG AGTATGGCCC TCACGCCGAC AGCCCTGTGC TCATGGTCTA TGGCTTGGAT CAATCTAAGA TGAACTGTGA CCGAGTCTTC AATGTCTTCT GCTTATATGG CAATGTGGAG AAGGTGAAAT TCATGAAAAG CAAGCCGGGG GCCGCCATGG TGGAGATGGC TGATGGCTAC GCTGTAGACC GGGCCATTAC CCACCTCAAC AACAACTTCA TGTTTGGGCA GAAGCTGAAT GTCTGTGTCT CCAAGCAGCC AGCCATCATG CCTGGTCAGT CATACGGGTT GGAAGACGGG TCTTGCAGTT ACAAAGACTT CAGTGAATCC CGGAACAATC GGTTCTCCAC CCCAGAGCAG GCAGCCAAGA ACCGCATCCA GCACCCCAGC AACGTGCTGC ACTTCTTCAA CGCCCCGCTG GAGGTGACCG AGGAGAACTT CTTTGAGATC TGCGATGAGC TGGGAGTGAA GCGGCCATCT TCTGTGAAAG TATTCTCAGG CAAAAGTGAG CGCAGCTCCT CTGGACTGCT GGAGTGGGAA TCCAAGAGCG ATGCCCTGGA GACTCTGGGC TTCCTGAACC ATTACCAGAT GAAAAACCCA AATGGTCCAT ACCCTTACAC TCTGAAGTTG TGTTTCTCCA CTGCTCAGCA CGCCTCCTAA GAATTCAGTC TAAGCTAGCA GATCTTCTAG AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGCGGCCGC AAATGCTAAA CCACTGCAGT GGTTACCAGT GCTTGATCAG TGAGGCACCG ATCTCAGCGA TCTGCCTATT TCGTTCGTCC ATAGTGGCCT GACTCCCCGT CGTGTAGATC ACTACGATTC GTGAGGGCTT ACCATCAGGC CCCAGCGCAG CAATGATGCC GCGAGAGCCG CGTTCACCGG CCCCCGATTT GTCAGCAATG AACCAGCCAG CAGGGAGGGC CGAGCGAAGA AGTGGTCCTG CTACTTTGTC CGCCTCCATC CAGTCTATGA GCTGCTGTCG TGATGCTAGA GTAAGAAGTT CGCCAGTGAG TAGTTTCCGA AGAGTTGTGG CCATTGCTAC TGGCATCGTG GTATCACGCT CGTCGTTCGG TATGGCTTCG TTCAACTCTG GTTCCCAGCG GTCAAGCCGG GTCACATGAT CACCCATATT ATGAAGAAAT GCAGTCAGCT CCTTAGGGCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC GGTGTTGTCG CTCATGGTAA TGGCAGCACT ACACAATTCT CTTACCGTCA TGCCATCCGT AAGATGCTTT TCCGTGACCG GCGAGTACTC AACCAAGTCG TTTTGTGAGT AGTGTATACG GCGACCAAGC TGCTCTTGCC CGGCGTCTAT ACGGGACAAC ACCGCGCCAC ATAGCAGTAC TTTGAAAGTG CTCATCATCG GGAATCGTTC TTCGGGGCGG AAAGACTCAA GGATCTTGCC GCTATTGAGA TCCAGTTCGA TATAGCCCAC TCTTGCACCC AGTTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCGG GGTGTGCAAA AACAGGCAAG CAAAATGCCG CAAAGAAGGG AATGAGTGCG ACACGAAAAT GTTGGATGCT CATACTCGTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTACTAGT ACGTCTCTCA AGGATAAGTA AGTAATATTA AGGTACGGGA GGTATTGGAC AGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGTACTA ACATACGCTC TCCATCAAAA CAAAACGAAA CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG AACATTTCTC T
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