pGL4.11-SHMT2(human)-Promoter-Fluc-hPEST
$84.00
In stock
SKU
PL56508
100μg liquid plasmid(Instantly):$105
| name | pGL4.11-SHMT2(human)-Promoter-Fluc-hPEST | another name | apGL4.11-SHMT2(human)-Promoter-Fluc-hPEST |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCT ACTCCTGGAG GGGTTGATGA ATCCCATTCC CAAGGGTTTC TTCCGGATGA AGTCAGGCTA AAAGGAAGAT CCCTTCCAGT CTCAAAGATC TATAGCCCTG GGGAAAAAAG GAGAGCCCAC CGACTCCTGG GACATCAAGA GGTGGACAGG TAACAAAATG GGACAAGTGA ACAACAGGAG GGGTGCCTTC CTCTGCTCCC AGATGTCTGA GGAGAAAGAG GAGATGCCAT GTAGGAGGCA GGTCTGTGGG TCAGAGGAAG AGGGGAGGAC AATCTGTCAC AGCGCATGTT CACAGGAAAA GCCTCATTGA ATCCTCACCA CACAGTATTT TCTTGTGGCT TTACCAGGGA GGAAACTGAA GCCCACACAA GCTAATGCAC TAGTCCCAGA GCCGGAACTT GAATTCAGGT CTTCCGACCC TTCTAAACCA CGGTGCTTTC CAGTGTTCCA TAGCTCTTTA TCTTGTGTGG GGGTCTAGAT CTCCTGGAGT CTAATAATGC TAGCTAGATA TTCAGTAGGC ACCCTATAAA CGTTTGTCTT GACAACAAGC AGAGAGAAAA CCCTGAAAAG CTTCAAGATA GTAAGGAGAG GGGGCGGCAC TGGACCTCTG TTTTTGTTTT TTGTTTTTTT CCCCCCAGGC AAGGGCCTCT AACCCTGGGG CTACAGACGG GAATCATAAA GAAAAAAGCG GTGAGTGGGC GAACTACAAT TCCCAAAAGG CCACAAAGGG GCCACCACTA CGCATGCGTA GATCCCTCCC GTTAGCTTTG GCGCCTCAGC GAGCTCTTCT CGCGCATGCG TTCTCCGAAC GGTCTTCTTC CGACAGCTTG CTGCCCTAGA CCAGAGTTGG TGGCTGGACC TCCTGCGACT TCCGAGTTGC GATGCTGTAC TTCTCTTTGA AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA CCAGATCATC CCCGACACCG CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT TCTGATCACC CCCGAAGGGG ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT AGCCCCAGCC GAACTGGAGA GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC CGTGAATTCT CACGGCTTCC CTCCCGAGGT GGAGGAGCAG GCCGCCGGCA CCCTGCCCAT GAGCTGCGCC CAGGAGAGCG GCATGGATAG ACACCCTGCT GCTTGCGCCA GCGCCAGGAT CAACGTCTAA GGCCGCGACT CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCGTC GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGCGG CCGCAAATGC TAAACCACTG CAGTGGTTAC CAGTGCTTGA TCAGTGAGGC ACCGATCTCA GCGATCTGCC TATTTCGTTC GTCCATAGTG GCCTGACTCC CCGTCGTGTA GATCACTACG ATTCGTGAGG GCTTACCATC AGGCCCCAGC GCAGCAATGA TGCCGCGAGA GCCGCGTTCA CCGGCCCCCG ATTTGTCAGC AATGAACCAG CCAGCAGGGA GGGCCGAGCG AAGAAGTGGT CCTGCTACTT TGTCCGCCTC CATCCAGTCT ATGAGCTGCT GTCGTGATGC TAGAGTAAGA AGTTCGCCAG TGAGTAGTTT CCGAAGAGTT GTGGCCATTG CTACTGGCAT CGTGGTATCA CGCTCGTCGT TCGGTATGGC TTCGTTCAAC TCTGGTTCCC AGCGGTCAAG CCGGGTCACA TGATCACCCA TATTATGAAG AAATGCAGTC AGCTCCTTAG GGCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCGGTGTT GTCGCTCATG GTAATGGCAG CACTACACAA TTCTCTTACC GTCATGCCAT CCGTAAGATG CTTTTCCGTG ACCGGCGAGT ACTCAACCAA GTCGTTTTGT GAGTAGTGTA TACGGCGACC AAGCTGCTCT TGCCCGGCGT CTATACGGGA CAACACCGCG CCACATAGCA GTACTTTGAA AGTGCTCATC ATCGGGAATC GTTCTTCGGG GCGGAAAGAC TCAAGGATCT TGCCGCTATT GAGATCCAGT TCGATATAGC CCACTCTTGC ACCCAGTTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCGGGGTGTG CAAAAACAGG CAAGCAAAAT GCCGCAAAGA AGGGAATGAG TGCGACACGA AAATGTTGGA TGCTCATACT CGTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAC TAGTACGTCT CTCAAGGATA AGTAAGTAAT ATTAAGGTAC GGGAGGTATT GGACAGGCCG CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT TTTTTGTGTG AATCGATAGT ACTAACATAC GCTCTCCATC AAAACAAAAC GAAACAAAAC AAACTAGCAA AATAGGCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT TCTCT
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