pDIRECT23A-AtU6-FPA-sgRNA1-sgRNA2-35S-Cas9
$113.00
In stock
SKU
PL56329
100μg liquid plasmid(Instantly):$134
| name | pDIRECT23A-AtU6-FPA-sgRNA1-sgRNA2-35S-Cas9 | another name | apDIRECT23A-AtU6-FPA-sgRNA1-sgRNA2-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | AtU6 | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC ATTCTAGGTA CTAAAACAAT TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT TGAAACCGCG GTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGAAT TAACGCCGAA TTAATTCGGG GGATCTGGAT TTTAGTACTG GATTTTGGTT TTAGGAATTA GAAATTTTAT TGATAGAAGT ATTTTACAAA TACAAATACA TACTAAGGGT TTCTTATATG CTCAACACAT GAGCGAAACC CTATAGGAAC CCTAATTCCC TTATCTGGGA ACTACTCACA CATTATTATG GAGAAACTCG AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGATG GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC GGCCGGGCGT CGTTCTGGGC TCATGGTAGA CTCGAGAGAG ATAGATTTGT AGAGAGAGAC TGGTGATTTC AGCGTGTCCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGGTCT TGCGAAGGAT AGTGGGATTG TGCGTCATCC CTTACGTCAG TGGAGATATC ACATCAATCC ACTTGCTTTG AAGACGTGGT TGGAACGTCT TCTTTTTCCA CGATGCTCCT CGTGGGTGGG GGTCCATCTT TGGGACCACT GTCGGCAGAG GCATCTTGAA CGATAGCCTT TCCTTTATCG CAATGATGGC ATTTGTAGGT GCCACCTTCC TTTTCTACTG TCCTTTTGAT GAAGTGACAG ATAGCTGGGC AATGGAATCC GAGGAGGTTT CCCGATATTA CCCTTTGTTG AAAAGTCTCA ATAGCCCTTT GGTCTTCTGA GACTGTATCT TTGATATTCT TGGAGTAGAC GAGAGTGTCG TGCTCCACCA TGTTCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAACGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGTGCCAC CTTCCTTTTC TACTGTCCTT TTGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA TATTACCCTT TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTCTTGGAG TAGACGAGAG TGTCGTGCTC CACCATGTTG GCAAGCTGCT CTAGCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC ATGATTACGA ATTCGAGCTC GGTACCCGGG GATCGGCGCG CCAGATTTGC CTTTTCAATT TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGGT ATCATCAAGA CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACTCCTGCA GGTAGATCGC TCGTCGACAT GGATAAGAAG TACTCTATCG GACTCGATAT CGGAACTAAC TCTGTGGGAT GGGCTGTGAT CACCGATGAG TACAAGGTGC CATCTAAGAA GTTCAAGGTT CTCGGAAACA CCGATAGGCA CTCTATCAAG AAAAACCTTA TCGGTGCTCT CCTCTTCGAT TCTGGTGAAA CTGCTGAGGC TACCAGACTC AAGAGAACCG CTAGAAGAAG GTACACCAGA AGAAAGAACA GGATCTGCTA CCTCCAAGAG ATCTTCTCTA ACGAGATGGC TAAAGTGGAT GATTCATTCT TCCACAGGCT CGAAGAGTCA TTCCTCGTGG AAGAAGATAA GAAGCACGAG AGGCACCCTA TCTTCGGAAA CATCGTTGAT GAGGTGGCAT ACCACGAGAA GTACCCTACT ATCTACCACC TCAGAAAGAA GCTCGTTGAT TCTACTGATA AGGCTGATCT CAGGCTCATC TACCTCGCTC TCGCTCACAT GATCAAGTTC AGAGGACACT TCCTCATCGA GGGTGATCTC AACCCTGATA ACTCTGATGT GGATAAGTTG TTCATCCAGC TCGTGCAGAC CTACAACCAG CTTTTCGAAG AGAACCCTAT CAACGCTTCA GGTGTGGATG CTAAGGCTAT CCTCTCTGCT AGGCTCTCTA AGTCAAGAAG GCTTGAGAAC CTCATTGCTC AGCTCCCTGG TGAGAAGAAG AACGGACTTT TCGGAAACTT GATCGCTCTC TCTCTCGGAC TCACCCCTAA CTTCAAGTCT AACTTCGATC TCGCTGAGGA TGCAAAGCTC CAGCTCTCAA AGGATACCTA CGATGATGAT CTCGATAACC TCCTCGCTCA GATCGGAGAT CAGTACGCTG ATTTGTTCCT CGCTGCTAAG AACCTCTCTG ATGCTATCCT CCTCAGTGAT ATCCTCAGAG TGAACACCGA GATCACCAAG GCTCCACTCT CAGCTTCTAT GATCAAGAGA TACGATGAGC ACCACCAGGA TCTCACACTT CTCAAGGCTC TTGTTAGACA GCAGCTCCCA GAGAAGTACA AAGAGATTTT CTTCGATCAG TCTAAGAACG GATACGCTGG TTACATCGAT GGTGGTGCAT CTCAAGAAGA GTTCTACAAG TTCATCAAGC CTATCCTCGA GAAGATGGAT GGAACCGAGG AACTCCTCGT GAAGCTCAAT AGAGAGGATC TTCTCAGAAA GCAGAGGACC TTCGATAACG GATCTATCCC TCATCAGATC CACCTCGGAG AGTTGCACGC TATCCTTAGA AGGCAAGAGG ATTTCTACCC ATTCCTCAAG GATAACAGGG AAAAGATTGA GAAGATTCTC ACCTTCAGAA TCCCTTACTA CGTGGGACCT CTCGCTAGAG GAAACTCAAG ATTCGCTTGG ATGACCAGAA AGTCTGAGGA AACCATCACC CCTTGGAACT TCGAAGAGGT GGTGGATAAG GGTGCTAGTG CTCAGTCTTT CATCGAGAGG ATGACCAACT TCGATAAGAA CCTTCCAAAC GAGAAGGTGC TCCCTAAGCA CTCTTTGCTC TACGAGTACT TCACCGTGTA CAACGAGTTG ACCAAGGTTA AGTACGTGAC CGAGGGAATG AGGAAGCCTG CTTTTTTGTC AGGTGAGCAA AAGAAGGCTA TCGTTGATCT CTTGTTCAAG ACCAACAGAA AGGTGACCGT GAAGCAGCTC AAAGAGGATT ACTTCAAGAA AATCGAGTGC TTCGATTCAG TTGAGATTTC TGGTGTTGAG GATAGGTTCA ACGCATCTCT CGGAACCTAC CACGATCTCC TCAAGATCAT TAAGGATAAG GATTTCTTGG ATAACGAGGA AAACGAGGAT ATCTTGGAGG ATATCGTTCT TACCCTCACC CTCTTTGAAG ATAGAGAGAT GATTGAAGAA AGGCTCAAGA CCTACGCTCA TCTCTTCGAT GATAAGGTGA TGAAGCAGTT GAAGAGAAGA AGATACACTG GTTGGGGAAG GCTCTCAAGA AAGCTCATTA ACGGAATCAG GGATAAGCAG TCTGGAAAGA CAATCCTTGA TTTCCTCAAG TCTGATGGAT TCGCTAACAG AAACTTCATG CAGCTCATCC ACGATGATTC TCTCACCTTT AAAGAGGATA TCCAGAAGGC TCAGGTTTCA GGACAGGGTG ATAGTCTCCA TGAGCATATC GCTAACCTCG CTGGATCTCC TGCAATCAAG AAGGGAATCC TCCAGACTGT GAAGGTTGTG GATGAGTTGG TGAAGGTGAT GGGAAGGCAT AAGCCTGAGA ACATCGTGAT CGAAATGGCT AGAGAGAACC AGACCACTCA GAAGGGACAG AAGAACTCTA GGGAAAGGAT GAAGAGGATC GAGGAAGGTA TCAAAGAGCT TGGATCTCAG ATCCTCAAAG AGCACCCTGT TGAGAACACT CAGCTCCAGA ATGAGAAGCT CTACCTCTAC TACCTCCAGA ACGGAAGGGA TATGTATGTG GATCAAGAGT TGGATATCAA CAGGCTCTCT GATTACGATG TTGATCATAT CGTGCCACAG TCATTCTTGA AGGATGATTC TATCGATAAC AAGGTGCTCA CCAGGTCTGA TAAGAACAGG GGTAAGAGTG ATAACGTGCC AAGTGAAGAG GTTGTGAAGA AAATGAAGAA CTATTGGAGG CAGCTCCTCA ACGCTAAGCT CATCACTCAG AGAAAGTTCG ATAACTTGAC TAAGGCTGAG AGGGGAGGAC TCTCTGAATT GGATAAGGCA GGATTCATCA AGAGGCAGCT TGTGGAAACC AGGCAGATCA CTAAGCACGT TGCACAGATC CTCGATTCTA GGATGAACAC CAAGTACGAT GAGAACGATA AGTTGATCAG GGAAGTGAAG GTTATCACCC TCAAGTCAAA GCTCGTGTCT GATTTCAGAA AGGATTTCCA ATTCTACAAG GTGAGGGAAA TCAACAACTA CCACCACGCT CACGATGCTT ACCTTAACGC TGTTGTTGGA ACCGCTCTCA TCAAGAAGTA TCCTAAGCTC GAGTCAGAGT TCGTGTACGG TGATTACAAG GTGTACGATG TGAGGAAGAT GATCGCTAAG TCTGAGCAAG AGATCGGAAA GGCTACCGCT AAGTATTTCT TCTACTCTAA CATCATGAAT TTCTTCAAGA CCGAGATTAC CCTCGCTAAC GGTGAGATCA GAAAGAGGCC ACTCATCGAG ACAAACGGTG AAACAGGTGA GATCGTGTGG GATAAGGGAA GGGATTTCGC TACCGTTAGA AAGGTGCTCT CTATGCCACA GGTGAACATC GTTAAGAAAA CCGAGGTGCA GACCGGTGGA TTCTCTAAAG AGTCTATCCT CCCTAAGAGG AACTCTGATA AGCTCATTGC TAGGAAGAAG GATTGGGACC CTAAGAAATA CGGTGGTTTC GATTCTCCTA CCGTGGCTTA CTCTGTTCTC GTTGTGGCTA AGGTTGAGAA GGGAAAGAGT AAGAAGCTCA AGTCTGTTAA GGAACTTCTC GGAATCACTA TCATGGAAAG GTCATCTTTC GAGAAGAACC CAATCGATTT CCTCGAGGCT AAGGGATACA AAGAGGTTAA GAAGGATCTC ATCATCAAGC TCCCAAAGTA CTCACTCTTC GAACTCGAGA ACGGTAGAAA GAGGATGCTC GCTTCTGCTG GTGAGCTTCA AAAGGGAAAC GAGCTTGCTC TCCCATCTAA GTACGTTAAC TTTCTTTACC TCGCTTCTCA CTACGAGAAG TTGAAGGGAT CTCCAGAAGA TAACGAGCAG AAGCAACTTT TCGTTGAGCA GCACAAGCAC TACTTGGATG AGATCATCGA GCAGATCTCT GAGTTCTCTA AAAGGGTGAT CCTCGCTGAT GCAAACCTCG ATAAGGTGTT GTCTGCTTAC AACAAGCACA GAGATAAGCC TATCAGGGAA CAGGCAGAGA ACATCATCCA TCTCTTCACC CTTACCAACC TCGGTGCTCC TGCTGCTTTC AAGTACTTCG ATACAACCAT CGATAGGAAG AGATACACCT CTACCAAAGA AGTGCTCGAT GCTACCCTCA TCCATCAGTC TATCACTGGA CTCTACGAGA CTAGGATCGA TCTCTCACAG CTCGGTGGTG ATTCAAGGGC TGATCCTAAG AAGAAGAGGA AGGTTTGACT CGAGATATGA AGATGAAGAT GAAATATTTG GTGTGTCAAA TAAAAGTGCC AAGCTTCGAC TTGCCTTCCG CACAATACAT CATTTCTTCT TAGCTTTTTT TCTTCTTCTT CGTTCATACA GTTTTTTTTT GTTTATCAGC TTACATTTTC TTGAACCGTA GCTTTCGTTT TCTTCTTTTT AACTTTCCAT TCGGAGTTTT TGTATCTTGT TTCATAGTTT GTCCCAGGAT TAGAATGATT AGGCATCGAA CCTTCAAGAA TTTGATTGAA TAAAACATCT TCATTCTTAA GATATGAAGA TAATCTTCAA AAGGCCCCTG GGAATCTGAA AGAAGAGAAG CAGGCCCATT TATATGGGAA AGAACAATAG TATTTCTTAT ATAGGCCCAT TTAAGTTGAA AACAATCTTC AAAAGTCCCA CATCGCTTAG ATAAGAAAAC GAAGCTGAGT TTATATACAG CTAGAGTCGA AGTAGTGATT GCTCTTCCTC CAGGTCATCC TGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTGCAAA ATTTTCCAGA TCGATTTCTT CTTCCTCTGT TCTTCGGCGC ATCGCTACCT TAGGACCGTT ATAGTTAATT ACCCTGTTAT CCCTATTAAT TAAGAGCTCA GAAATCTCAA AATTCCGGCA GAACAATTTT GAATCTCGAT CCGTAGAAAC GAGACGGTCA TTGTTTTAGT TCCACCACGA TTATATTTGA AATTTACGTG AGTGTGAGTG AGACTTGCAT AAGAAAATAA AATCTTTAGT TGGGAAAAAA TTCAATAATA TAAATGGGCT TGAGAAGGAA GCGAGGGATA GGCCTTTTTC TAAAATAGGC CCATTTAAGC TATTAACAAT CTTCAAAAGT ACCACAGCGC TTAGGTAAAG AAAGCAGCTG AGTTTATATA TGGTTAGAGA CGAAGTAGTG ATTGTCCACA GAACCTTACT TGGCGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTTCTA GACCCAGCTT TCTTGTACGT TTAAACTATC AGTGTTTGAC AGGATATATT GGCGGGTAAA CCTAAGAGAA AAGAGCGTTT ATTAGAATAA TCGGATATTT AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT CCATTTGTAT GTGCATGCCA ACCACAGGGT TCCCCTCGGG ATCAAAGTAC TTTGATCCAA CCCCTCCGCT GCTATAGTGC AGTCGGCTTC TGACGTTCAG TGCAGCCGTC TTCTGAAAAC GACATGTCGC ACAAGTCCTA AGTTACGCGA CAGGCTGCCG CCCTGCCCTT TTCCTGGCGT TTTCTTGTCG CGTGTTTTAG TCGCATAAAG TAGAATACTT GCGACTAGAA CCGGAGACAT TACGCCATGA ACAAGAGCGC CGCCGCTGGC CTGCTGGGCT ATGCCCGCGT CAGCACCGAC GACCAGGACT TGACCAACCA ACGGGCCGAA CTGCACGCGG CCGGCTGCAC CAAGCTGTTT TCCGAGAAGA TCACCGGCAC CAGGCGCGAC CGCCCGGAGC TGGCCAGGAT GCTTGACCAC CTACGCCCTG GCGACGTTGT GACAGTGACC AGGCTAGACC GCCTGGCCCG CAGCACCCGC GACCTACTGG ACATTGCCGA GCGCATCCAG GAGGCCGGCG CGGGCCTGCG TAGCCTGGCA GAGCCGTGGG CCGACACCAC CACGCCGGCC GGCCGCATGG TGTTGACCGT GTTCGCCGGC ATTGCCGAGT TCGAGCGTTC CCTAATCATC GACCGCACCC GGAGCGGGCG CGAGGCCGCC AAGGCCCGAG GCGTGAAGTT TGGCCCCCGC CCTACCCTCA CCCCGGCACA GATCGCGCAC GCCCGCGAGC TGATCGACCA GGAAGGCCGC ACCGTGAAAG AGGCGGCTGC ACTGCTTGGC GTGCATCGCT CGACCCTGTA CCGCGCACTT GAGCGCAGCG AGGAAGTGAC GCCCACCGAG GCCAGGCGGC GCGGTGCCTT CCGTGAGGAC GCATTGACCG AGGCCGACGC CCTGGCGGCC GCCGAGAATG AACGCCAAGA GGAACAAGCA TGAAACCGCA CCAGGACGGC CAGGACGAAC CGTTTTTCAT TACCGAAGAG ATCGAGGCGG AGATGATCGC GGCCGGGTAC GTGTTCGAGC CGCCCGCGCA CGTCTCAACC GTGCGGCTGC ATGAAATCCT GGCCGGTTTG TCTGATGCCA AGCTGGCGGC CTGGCCGGCC AGCTTGGCCG CTGAAGAAAC CGAGCGCCGC CGTCTAAAAA GGTGATGTGT ATTTGAGTAA AACAGCTTGC GTCATGCGGT CGCTGCGTAT ATGATGCGAT GAGTAAATAA ACAAATACGC AAGGGGAACG CATGAAGGTT ATCGCTGTAC TTAACCAGAA AGGCGGGTCA GGCAAGACGA CCATCGCAAC CCATCTAGCC CGCGCCCTGC AACTCGCCGG GGCCGATGTT CTGTTAGTCG ATTCCGATCC CCAGGGCAGT GCCCGCGATT GGGCGGCCGT GCGGGAAGAT CAACCGCTAA CCGTTGTCGG CATCGACCGC CCGACGATTG ACCGCGACGT GAAGGCCATC GGCCGGCGCG ACTTCGTAGT GATCGACGGA GCGCCCCAGG CGGCGGACTT GGCTGTGTCC GCGATCAAGG CAGCCGACTT CGTGCTGATT CCGGTGCAGC CAAGCCCTTA CGACATATGG GCCACCGCCG ACCTGGTGGA GCTGGTTAAG CAGCGCATTG AGGTCACGGA TGGAAGGCTA CAAGCGGCCT TTGTCGTGTC GCGGGCGATC AAAGGCACGC GCATCGGCGG TGAGGTTGCC GAGGCGCTGG CCGGGTACGA GCTGCCCATT CTTGAGTCCC GTATCACGCA GCGCGTGAGC TACCCAGGCA CTGCCGCCGC CGGCACAACC GTTCTTGAAT CAGAACCCGA GGGCGACGCT GCCCGCGAGG TCCAGGCGCT GGCCGCTGAA ATTAAATCAA AACTCATTTG AGTTAATGAG GTAAAGAGAA AATGAGCAAA AGCACAAACA CGCTAAGTGC CGGCCGTCCG AGCGCACGCA GCAGCAAGGC TGCAACGTTG GCCAGCCTGG CAGACACGCC AGCCATGAAG CGGGTCAACT TTCAGTTGCC GGCGGAGGAT CACACCAAGC TGAAGATGTA CGCGGTACGC CAAGGCAAGA CCATTACCGA GCTGCTATCT GAATACATCG CGCAGCTACC AGAGTAAATG AGCAAATGAA TAAATGAGTA GATGAATTTT AGCGGCTAAA GGAGGCGGCA TGGAAAATCA AGAACAACCA GGCACCGACG CCGTGGAATG CCCCATGTGT GGAGGAACGG GCGGTTGGCC AGGCGTAAGC GGCTGGGTTG CCTGCCGGCC CTGCAATGGC ACTGGAACCC CCAAGCCCGA GGAATCGGCG TGAGCGGTCG CAAACCATCC GGCCCGGTAC AAATCGGCGC GGCGCTGGGT GATGACCTGG TGGAGAAGTT GAAGGCCGCG CAGGCCGCCC AGCGGCAACG CATCGAGGCA GAAGCACGCC CCGGTGAATC GTGGCAAGCG GCCGCTGATC GAATCCGCAA AGAATCCCGG CAACCGCCGG CAGCCGGTGC GCCGTCGATT AGGAAGCCGC CCAAGGGCGA CGAGCAACCA GATTTTTTCG TTCCGATGCT CTATGACGTG GGCACCCGCG ATAGTCGCAG CATCATGGAC GTGGCCGTTT TCCGTCTGTC GAAGCGTGAC CGACGAGCTG GCGAGGTGAT CCGCTACGAG CTTCCAGACG GGCACGTAGA GGTTTCCGCA GGGCCGGCCG GCATGGCCAG TGTGTGGGAT TACGACCTGG TACTGATGGC GGTTTCCCAT CTAACCGAAT CCATGAACCG ATACCGGGAA GGGAAGGGAG ACAAGCCCGG CCGCGTGTTC CGTCCACACG TTGCGGACGT ACTCAAGTTC TGCCGGCGAG CCGATGGCGG AAAGCAGAAA GACGACCTGG TAGAAACCTG CATTCGGTTA AACACCACGC ACGTTGCCAT GCAGCGTACG AAGAAGGCCA AGAACGGCCG CCTGGTGACG GTATCCGAGG GTGAAGCCTT GATTAGCCGC TACAAGATCG TAAAGAGCGA AACCGGGCGG CCGGAGTACA TCGAGATCGA GCTAGCTGAT TGGATGTACC GCGAGATCAC AGAAGGCAAG AACCCGGACG TGCTGACGGT TCACCCCGAT TACTTTTTGA TCGATCCCGG CATCGGCCGT TTTCTCTACC GCCTGGCACG CCGCGCCGCA GGCAAGGCAG AAGCCAGATG GTTGTTCAAG ACGATCTACG AACGCAGTGG CAGCGCCGGA GAGTTCAAGA AGTTCTGTTT CACCGTGCGC AAGCTGATCG GGTCAAATGA CCTGCCGGAG TACGATTTGA AGGAGGAGGC GGGGCAGGCT GGCCCGATCC TAGTCATGCG CTACCGCAAC CTGATCGAGG GCGAAGCATC CGCCGGTTCC TAATGTACGG AGCAGATGCT AGGGCAAATT GCCCTAGCAG GGGAAAAAGG TCGAAAAGGT CTCTTTCCTG TGGATAGCAC GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGAAC CCGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGT CACACATGTA AGTGACTGAT ATAAAAGAGA AAAAAGGCGA TTTTTCCGCC TAAAACTCTT TAAAACTTAT TAAAACTCTT AAAACCCGCC TGGCCTGTGC ATAACTGTCT GGCCAGCGCA CAGCCCAAGA GCTGCAAAAA GCGCCTACCC TTCGGTCGCT GCGCTCCCTA CGCCCCGCCG CTTCGCGTCG GCCTATCGCG GCCGCTGGCC GCTCAAAAAT GGCTGGCCTA CGGCCAGGCA ATCTACCAGG GCGCGGACAA GCCGCGCCGT CGCCACTCGA CCGCCGGCGC CCACATCAAG GCACCCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCCC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTAT
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