pHEE401E-FPA-sgRNA1-FPA-sgRNA2
$84.00
In stock
SKU
PL56320
100μg liquid plasmid(Instantly):$105
| name | pHEE401E-FPA-sgRNA1-FPA-sgRNA2 | another name | apHEE401E-FPA-sgRNA1-FPA-sgRNA2 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTTCG ACTTGCCTTC CGCACAATAC ATCATTTCTT CTTAGCTTTT TTTCTTCTTC TTCGTTCATA CAGTTTTTTT TTGTTTATCA GCTTACATTT TCTTGAACCG TAGCTTTCGT TTTCTTCTTT TTAACTTTCC ATTCGGAGTT TTTGTATCTT GTTTCATAGT TTGTCCCAGG ATTAGAATGA TTAGGCATCG AACCTTCAAG AATTTGATTG AATAAAACAT CTTCATTCTT AAGATATGAA GATAATCTTC AAAAGGCCCC TGGGAATCTG AAAGAAGAGA AGCAGGCCCA TTTATATGGG AAAGAACAAT AGTATTTCTT ATATAGGCCC ATTTAAGTTG AAAACAATCT TCAAAAGTCC CACATCGCTT AGATAAGAAA ACGAAGCTGA GTTTATATAC AGCTAGAGTC GAAGTAGTGA TTGCTCTTCC TCCAGGTCAT CCTGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCT TTTTTTTGCA AAATTTTCCA GATCGATTTC TTCTTCCTCT GTTCTTCGGC CATCGCTACC TTAGGACCGT TATAGTTAAT TACCCTGTTA TCCCTATTAA TTAAGAGCTC AGAAATCTCA AAATTCCGGC AGAACAATTT TGAATCTCGA TCCGTAGAAA CGAGACGGTC ATTGTTTTAG TTCCACCACG ATTATATTTG AAATTTACGT GAGTGTGAGT GAGACTTGCA TAAGAAAATA AAATCTTTAG TTGGGAAAAA ATTCAATAAT ATAAATGGGC TTGAGAAGGA AGCGAGGGAT AGGCCTTTTT CTAAAATAGG CCCATTTAAG CTATTAACAA TCTTCAAAAG TACCACAGCG CTTAGGTAAA GAAAGCAGCT GAGTTTATAT ATGGTTAGAG ACGAAGTAGT GATTGTCCAC AGAACCTTAC TTGGCGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTCT AGACCCAGCT TTCTTGTACA CTAGTGAATA AAAGCATTTG CGTTTGGTTT ATCATTGCGT TTATACAAGG ACAGAGATCC ACTGAGCTGG AATAGCTTAA AACCATTATC AGAACAAAAT AAACCATTTT TTGTTAAGAA TCAGAGCATA GTAAACAACA GAAACAACCT AAGAGAGGTA ACTTGTCCAA GAAGATAGCT AATTATATCT ATTTTATAAA AGTTATCATA GTTTGTAAGT CACAAAAGAT GCAAATAACA GAGAAACTAG GAGACTTGAG AATATACATT CTTGTATATT TGTATTCGAG ATTGTGAAAA TTTGACCATA AGTTTAAATT CTTAAAAAGA TATATCTGAT CTAGGTGATG GTTATAGACT GTAATTTTAC CACATGTTTA ATGATGGATA GTGACACACA TGACACATCG ACAACACTAT AGCATCTTAT TTAGATTACA ACATGAAATT TTTCTGTAAT ACATGTCTTT GTACATAATT TAAAAGTAAT TCCTAAGAAA TATATTTATA CAAGGAGTTT AAAGAAAACA TAGCATAAAG TTCAATGAGT AGTAAAAACC ATATACAGTA TATAGCATAA AGTTCAATGA GTTTATTACA AAAGCATTGG TTCACTTTCT GTAACACGAC GTTAAACCTT CGTCTCCAAT AGGAGCGCTA CTGATTCAAC ATGCCAATAT ATACTAAATA CGTTTCTACA GTCAAATGCT TTAACGTTTC ATGATTAAGT GACTATTTAC CGTCAATCCT TTCCCATTCC TCCCACTAAT CCAACTTTTT AATTACTCTT AAATCACCAC TAAGCTAGTA ACGCCTATCA TGAATTAGCT CTACTAAATC TAGCAACCTT TCAAATTTGC AGTATTGCAG GTGTCTCTGT GTCTTTAAAA TAGTTGCCTT ATGATTTCTT CGGTTTCAAG ATGATCAAAT AGTTATAGAT TTCATGCTCA CACATGCTCA TTAGATGTGT ACATACTTTA CTTACCCAAA TCTATTTTCT CGCAAAGATT TTGATGGTAA AGCTGATTTG GTTCTATTGA ACTAAATCAA ACGAGTTTCA GACTGAGTGA TTCTAATCCG GCCCATTAGC CCCTAAACAG ACCCACTAAT TACGCAGCTT TTAATAGAGT AATTACACCT AGTTTACCCA CTAAACCACT AAGCACTAAT TATCTCACAA TCTAATGAGC TTCCCTCGTA ATTACTTGGG CTTTCACTCT ACCATTTATT TGTAACAGTC AAGTCTCTAC TGTCTCTATA TAAACTCTCT AAAGTTAACA CACAATTCTC ATCACAAACA AATCAACCAA AGCAACTTCT ACTCTTTCTT CTTTCGACCT TATCAATCTG TTGAGAAATC TAGATGGATT ACAAGGACCA CGACGGGGAT TACAAGGACC ACGACATTGA TTACAAGGAT GATGATGACA AGATGGCTCC GAAGAAGAAG AGGAAGGTTG GCATCCACGG GGTGCCAGCT GCTGACAAGA AGTACTCGAT CGGCCTCGAT ATTGGGACTA ACTCTGTTGG CTGGGCCGTG ATCACCGACG AGTACAAGGT GCCCTCAAAG AAGTTCAAGG TCCTGGGCAA CACCGATCGG CATTCCATCA AGAAGAATCT CATTGGCGCT CTCCTGTTCG ACAGCGGCGA GACGGCTGAG GCTACGCGGC TCAAGCGCAC CGCCCGCAGG CGGTACACGC GCAGGAAGAA TCGCATCTGC TACCTGCAGG AGATTTTCTC CAACGAGATG GCGAAGGTTG ACGATTCTTT CTTCCACAGG CTGGAGGAGT CATTCCTCGT GGAGGAGGAT AAGAAGCACG AGCGGCATCC AATCTTCGGC AACATTGTCG ACGAGGTTGC CTACCACGAG AAGTACCCTA CGATCTACCA TCTGCGGAAG AAGCTCGTGG ACTCCACAGA TAAGGCGGAC CTCCGCCTGA TCTACCTCGC TCTGGCCCAC ATGATTAAGT TCAGGGGCCA TTTCCTGATC GAGGGGGATC TCAACCCGGA CAATAGCGAT GTTGACAAGC TGTTCATCCA GCTCGTGCAG ACGTACAACC AGCTCTTCGA GGAGAACCCC ATTAATGCGT CAGGCGTCGA CGCGAAGGCT ATCCTGTCCG CTAGGCTCTC GAAGTCTCGG CGCCTCGAGA ACCTGATCGC CCAGCTGCCG GGCGAGAAGA AGAACGGCCT GTTCGGGAAT CTCATTGCGC TCAGCCTGGG GCTCACGCCC AACTTCAAGT CGAATTTCGA TCTCGCTGAG GACGCCAAGC TGCAGCTCTC CAAGGACACA TACGACGATG ACCTGGATAA CCTCCTGGCC CAGATCGGCG ATCAGTACGC GGACCTGTTC CTCGCTGCCA AGAATCTGTC GGACGCCATC CTCCTGTCTG ATATTCTCAG GGTGAACACC GAGATTACGA AGGCTCCGCT CTCAGCCTCC ATGATCAAGC GCTACGACGA GCACCATCAG GATCTGACCC TCCTGAAGGC GCTGGTCAGG CAGCAGCTCC CCGAGAAGTA CAAGGAGATC TTCTTCGATC AGTCGAAGAA CGGCTACGCT GGGTACATTG ACGGCGGGGC CTCTCAGGAG GAGTTCTACA AGTTCATCAA GCCGATTCTG GAGAAGATGG ACGGCACGGA GGAGCTGCTG GTGAAGCTCA ATCGCGAGGA CCTCCTGAGG AAGCAGCGGA CATTCGATAA CGGCAGCATC CCACACCAGA TTCATCTCGG GGAGCTGCAC GCTATCCTGA GGAGGCAGGA GGACTTCTAC CCTTTCCTCA AGGATAACCG CGAGAAGATC GAGAAGATTC TGACTTTCAG GATCCCGTAC TACGTCGGCC CACTCGCTAG GGGCAACTCC CGCTTCGCTT GGATGACCCG CAAGTCAGAG GAGACGATCA CGCCGTGGAA CTTCGAGGAG GTGGTCGACA AGGGCGCTAG CGCTCAGTCG TTCATCGAGA GGATGACGAA TTTCGACAAG AACCTGCCAA ATGAGAAGGT GCTCCCTAAG CACTCGCTCC TGTACGAGTA CTTCACAGTC TACAACGAGC TGACTAAGGT GAAGTATGTG ACCGAGGGCA TGAGGAAGCC GGCTTTCCTG TCTGGGGAGC AGAAGAAGGC CATCGTGGAC CTCCTGTTCA AGACCAACCG GAAGGTCACG GTTAAGCAGC TCAAGGAGGA CTACTTCAAG AAGATTGAGT GCTTCGATTC GGTCGAGATC TCTGGCGTTG AGGACCGCTT CAACGCCTCC CTGGGGACCT ACCACGATCT CCTGAAGATC ATTAAGGATA AGGACTTCCT GGACAACGAG GAGAATGAGG ATATCCTCGA GGACATTGTG CTGACACTCA CTCTGTTCGA GGACCGGGAG ATGATCGAGG AGCGCCTGAA GACTTACGCC CATCTCTTCG ATGACAAGGT CATGAAGCAG CTCAAGAGGA GGAGGTACAC CGGCTGGGGG AGGCTGAGCA GGAAGCTCAT CAACGGCATT CGGGACAAGC AGTCCGGGAA GACGATCCTC GACTTCCTGA AGAGCGATGG CTTCGCGAAC CGCAATTTCA TGCAGCTGAT TCACGATGAC AGCCTCACAT TCAAGGAGGA TATCCAGAAG GCTCAGGTGA GCGGCCAGGG GGACTCGCTG CACGAGCATA TCGCGAACCT CGCTGGCTCG CCAGCTATCA AGAAGGGGAT TCTGCAGACC GTGAAGGTTG TGGACGAGCT GGTGAAGGTC ATGGGCAGGC ACAAGCCTGA GAACATCGTC ATTGAGATGG CCCGGGAGAA TCAGACCACG CAGAAGGGCC AGAAGAACTC ACGCGAGAGG ATGAAGAGGA TCGAGGAGGG CATTAAGGAG CTGGGGTCCC AGATCCTCAA GGAGCACCCG GTGGAGAACA CGCAGCTGCA GAATGAGAAG CTCTACCTGT ACTACCTCCA GAATGGCCGC GATATGTATG TGGACCAGGA GCTGGATATT AACAGGCTCA GCGATTACGA CGTCGATCAT ATCGTTCCAC AGTCATTCCT GAAGGATGAC TCCATTGACA ACAAGGTCCT CACCAGGTCG GACAAGAACC GGGGCAAGTC TGATAATGTT CCTTCAGAGG AGGTCGTTAA GAAGATGAAG AACTACTGGC GCCAGCTCCT GAATGCCAAG CTGATCACGC AGCGGAAGTT CGATAACCTC ACAAAGGCTG AGAGGGGCGG GCTCTCTGAG CTGGACAAGG CGGGCTTCAT CAAGAGGCAG CTGGTCGAGA CACGGCAGAT CACTAAGCAC GTTGCGCAGA TTCTCGACTC ACGGATGAAC ACTAAGTACG ATGAGAATGA CAAGCTGATC CGCGAGGTGA AGGTCATCAC CCTGAAGTCA AAGCTCGTCT CCGACTTCAG GAAGGATTTC CAGTTCTACA AGGTTCGGGA GATCAACAAT TACCACCATG CCCATGACGC GTACCTGAAC GCGGTGGTCG GCACAGCTCT GATCAAGAAG TACCCAAAGC TCGAGAGCGA GTTCGTGTAC GGGGACTACA AGGTTTACGA TGTGAGGAAG ATGATCGCCA AGTCGGAGCA GGAGATTGGC AAGGCTACCG CCAAGTACTT CTTCTACTCT AACATTATGA ATTTCTTCAA GACAGAGATC ACTCTGGCCA ATGGCGAGAT CCGGAAGCGC CCCCTCATCG AGACGAACGG CGAGACGGGG GAGATCGTGT GGGACAAGGG CAGGGATTTC GCGACCGTCA GGAAGGTTCT CTCCATGCCA CAAGTGAATA TCGTCAAGAA GACAGAGGTC CAGACTGGCG GGTTCTCTAA GGAGTCAATT CTGCCTAAGC GGAACAGCGA CAAGCTCATC GCCCGCAAGA AGGACTGGGA TCCGAAGAAG TACGGCGGGT TCGACAGCCC CACTGTGGCC TACTCGGTCC TGGTTGTGGC GAAGGTTGAG AAGGGCAAGT CCAAGAAGCT CAAGAGCGTG AAGGAGCTGC TGGGGATCAC GATTATGGAG CGCTCCAGCT TCGAGAAGAA CCCGATCGAT TTCCTGGAGG CGAAGGGCTA CAAGGAGGTG AAGAAGGACC TGATCATTAA GCTCCCCAAG TACTCACTCT TCGAGCTGGA GAACGGCAGG AAGCGGATGC TGGCTTCCGC TGGCGAGCTG CAGAAGGGGA ACGAGCTGGC TCTGCCGTCC AAGTATGTGA ACTTCCTCTA CCTGGCCTCC CACTACGAGA AGCTCAAGGG CAGCCCCGAG GACAACGAGC AGAAGCAGCT GTTCGTCGAG CAGCACAAGC ATTACCTCGA CGAGATCATT GAGCAGATTT CCGAGTTCTC CAAGCGCGTG ATCCTGGCCG ACGCGAATCT GGATAAGGTC CTCTCCGCGT ACAACAAGCA CCGCGACAAG CCAATCAGGG AGCAGGCTGA GAATATCATT CATCTCTTCA CCCTGACGAA CCTCGGCGCC CCTGCTGCTT TCAAGTACTT CGACACAACT ATCGATCGCA AGAGGTACAC AAGCACTAAG GAGGTCCTGG ACGCGACCCT CATCCACCAG TCGATTACCG GCCTCTACGA GACGCGCATC GACCTGTCTC AGCTCGGGGG CGACAAGCGG CCAGCGGCGA CGAAGAAGGC GGGGCAGGCG AAGAAGAAGA AGTGAGCTCA GAGCTTTCGT TCGTATCATC GGTTTCGACA ACGTTCGTCA AGTTCAATGC ATCAGTTTCA TTGCGCACAC ACCAGAATCC TACTGAGTTT GAGTATTATG GCATTGGGAA AACTGTTTTT CTTGTACCAT TTGTTGTGCT TGTAATTTAC TGTGTTTTTT ATTCGGTTTT CGCTATCGAA CTGTGAAATG GAAATGGATG GAGAAGAGTT AATGAATGAT ATGGTCCTTT TGTTCATTCT CAAATTAATA TTATTTGTTT TTTCTCTTAT TTGTTGTGTG TTGAATTTGA AATTATAAGA GATATGCAAA CATTTTGTTT TGAGTAAAAA TGTGTCAAAT CGTGGCCTCT AATGACCGAA GTTAATATGA GGAGTAAAAC ACTTGTAGTT GTACCATTAT GCTTATTCAC TAGGCAACAA ATATATTTTC AGACCTAGAA AAGCTGCAAA TGTTACTGAA TACAAGTATG TCCTCTTGTG TTTTAGACAT TTATGAACTT TCCTTTATGT AATTTTCCAG AATCCTTGTC AGATTCTAAT CATTGCTTTA TAATTATAGT TATACTCATG GATTTGTAGT TGAGTATGAA AATATTTTTT AATGCATTTT ATGACTTGCC AATTGATTGA CAACGAATTC GTAATCATGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGCTAGAG CAGCTTGCCA ACATGGTGGA GCACGACACT CTCGTCTACT CCAAGAATAT CAAAGATACA GTCTCAGAAG ACCAAAGGGC TATTGAGACT TTTCAACAAA GGGTAATATC GGGAAACCTC CTCGGATTCC ATTGCCCAGC TATCTGTCAC TTCATCAAAA GGACAGTAGA AAAGGAAGGT GGCACCTACA AATGCCATCA TTGCGATAAA GGAAAGGCTA TCGTTCAAGA TGCCTCTGCC GACAGTGGTC CCAAAGATGG ACCCCCACCC ACGAGGAGCA TCGTGGAAAA AGAAGACGTT CCAACCACGT CTTCAAAGCA AGTGGATTGA TGTGAACATG GTGGAGCACG ACACTCTCGT CTACTCCAAG AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCTATTG AGACTTTTCA ACAAAGGGTA ATATCGGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTCAT CAAAAGGACA GTAGAAAAGG AAGGTGGCAC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCTATCGTT CAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG GACACGCTGA AATCACCAGT CTCTCTCTAC AAATCTATCT CTCTCGAGCT TTCGCAGATC CGGGGGGCAA TGAGATATGA AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AGTTTAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTTC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG CCCGCTGTTC TACAACCGGT CGCGGAGGCT ATGGATGCGA TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCACGAC TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGAGCCG GGACTGTCGG GCGTACACAA ATCGCCCGCA GAAGCGCGGC CGTCTGGACC GATGGCTGTG TAGAAGTACT CGCCGATAGT GGAAACCGAC GCCCCAGCAC TCGTCCGAGG GCAAAGAAAT AGAGTAGATG CCGACCGGGA TCTGTCGATC GACAAGCTCG AGTTTCTCCA TAATAATGTG TGAGTAGTTC CCAGATAAGG GAATTAGGGT TCCTATAGGG TTTCGCTCAT GTGTTGAGCA TATAAGAAAC CCTTAGTATG TATTTGTATT TGTAAAATAC TTCTATCAAT AAAATTTCTA ATTCCTAAAA CCAAAATCCA GTACTAAAAT CCAGATCCCC CGAATTAATT CGGCGTTAAT TCAGTACATT AAAAACGTCC GCAATGTGTT ATTAAGTTGT CTAAGCGTCA ATTTGTTTAC ACCACAATAT ATCCTGCCAC CAGCCAGCCA ACAGCTCCCC GACCGGCAGC TCGGCACAAA ATCACCACTC GATACAGGCA GCCCATCAGT CCGGGACGGC GTCAGCGGGA GAGCCGTTGT AAGGCGGCAG ACTTTGCTCA TGTTACCGAT GCTATTCGGA AGAACGGCAA CTAAGCTGCC GGGTTTGAAA CACGGATGAT CTCGCGGAGG GTAGCATGTT GATTGTAACG ATGACAGAGC GTTGCTGCCT GTGATCACCG CGGTTTCAAA ATCGGCTCCG TCGATACTAT GTTATACGCC AACTTTGAAA ACAACTTTGA AAAAGCTGTT TTCTGGTATT TAAGGTTTTA GAATGCAAGG AACAGTGAAT TGGAGTTCGT CTTGTTATAA TTAGCTTCTT GGGGTATCTT TAAATACTGT AGAAAAGAGG AAGGAAATAA TAAATGGCTA AAATGAGAAT ATCACCGGAA TTGAAAAAAC TGATCGAAAA ATACCGCTGC GTAAAAGATA CGGAAGGAAT GTCTCCTGCT AAGGTATATA AGCTGGTGGG AGAAAATGAA AACCTATATT TAAAAATGAC GGACAGCCGG TATAAAGGGA CCACCTATGA TGTGGAACGG GAAAAGGACA TGATGCTATG GCTGGAAGGA AAGCTGCCTG TTCCAAAGGT CCTGCACTTT GAACGGCATG ATGGCTGGAG CAATCTGCTC ATGAGTGAGG CCGATGGCGT CCTTTGCTCG GAAGAGTATG AAGATGAACA AAGCCCTGAA AAGATTATCG AGCTGTATGC GGAGTGCATC AGGCTCTTTC ACTCCATCGA CATATCGGAT TGTCCCTATA CGAATAGCTT AGACAGCCGC TTAGCCGAAT TGGATTACTT ACTGAATAAC GATCTGGCCG ATGTGGATTG CGAAAACTGG GAAGAAGACA CTCCATTTAA AGATCCGCGC GAGCTGTATG ATTTTTTAAA GACGGAAAAG CCCGAAGAGG AACTTGTCTT TTCCCACGGC GACCTGGGAG ACAGCAACAT CTTTGTGAAA GATGGCAAAG TAAGTGGCTT TATTGATCTT GGGAGAAGCG GCAGGGCGGA CAAGTGGTAT GACATTGCCT TCTGCGTCCG GTCGATCAGG GAGGATATCG GGGAAGAACA GTATGTCGAG CTATTTTTTG ACTTACTGGG GATCAAGCCT GATTGGGAGA AAATAAAATA TTATATTTTA CTGGATGAAT TGTTTTAGTA CCTAGAATGC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG GGTGCCTTGA TGTGGGCGCC GGCGGTCGAG TGGCGACGGC GCGGCTTGTC CGCGCCCTGG TAGATTGCCT GGCCGTAGGC CAGCCATTTT TGAGCGGCCA GCGGCCGCGA TAGGCCGACG CGAAGCGGCG GGGCGTAGGG AGCGCAGCGA CCGAAGGGTA GGCGCTTTTT GCAGCTCTTC GGCTGTGCGC TGGCCAGACA GTTATGCACA GGCCAGGCGG GTTTTAAGAG TTTTAATAAG TTTTAAAGAG TTTTAGGCGG AAAAATCGCC TTTTTTCTCT TTTATATCAG TCACTTACAT GTGTGACCGG TTCCCAATGT ACGGCTTTGG GTTCCCAATG TACGGGTTCC GGTTCCCAAT GTACGGCTTT GGGTTCCCAA TGTACGTGCT ATCCACAGGA AACAGACCTT TTCGACCTTT TTCCCCTGCT AGGGCAATTT GCCCTAGCAT CTGCTCCGTA CATTAGGAAC CGGCGGATGC TTCGCCCTCG ATCAGGTTGC GGTAGCGCAT GACTAGGATC GGGCCAGCCT GCCCCGCCTC CTCCTTCAAA TCGTACTCCG GCAGGTCATT TGACCCGATC AGCTTGCGCA CGGTGAAACA GAACTTCTTG AACTCTCCGG CGCTGCCACT GCGTTCGTAG ATCGTCTTGA ACAACCATCT GGCTTCTGCC TTGCCTGCGG CGCGGCGTGC CAGGCGGTAG AGAAAACGGC CGATGCCGGG ATCGATCAAA AAGTAATCGG GGTGAACCGT CAGCACGTCC GGGTTCTTGC CTTCTGTGAT CTCGCGGTAC ATCCAATCAG CTAGCTCGAT CTCGATGTAC TCCGGCCGCC CGGTTTCGCT CTTTACGATC TTGTAGCGGC TAATCAAGGC TTCACCCTCG GATACCGTCA CCAGGCGGCC GTTCTTGGCC TTCTTCGTAC GCTGCATGGC AACGTGCGTG GTGTTTAACC GAATGCAGGT TTCTACCAGG TCGTCTTTCT GCTTTCCGCC ATCGGCTCGC CGGCAGAACT TGAGTACGTC CGCAACGTGT GGACGGAACA CGCGGCCGGG CTTGTCTCCC TTCCCTTCCC GGTATCGGTT CATGGATTCG GTTAGATGGG AAACCGCCAT CAGTACCAGG TCGTAATCCC ACACACTGGC CATGCCGGCC GGCCCTGCGG AAACCTCTAC GTGCCCGTCT GGAAGCTCGT AGCGGATCAC CTCGCCAGCT CGTCGGTCAC GCTTCGACAG ACGGAAAACG GCCACGTCCA TGATGCTGCG ACTATCGCGG GTGCCCACGT CATAGAGCAT CGGAACGAAA AAATCTGGTT GCTCGTCGCC CTTGGGCGGC TTCCTAATCG ACGGCGCACC GGCTGCCGGC GGTTGCCGGG ATTCTTTGCG GATTCGATCA GCGGCCGCTT GCCACGATTC ACCGGGGCGT GCTTCTGCCT CGATGCGTTG CCGCTGGGCG GCCTGCGCGG CCTTCAACTT CTCCACCAGG TCATCACCCA GCGCCGCGCC GATTTGTACC GGGCCGGATG GTTTGCGACC GCTCACGCCG ATTCCTCGGG CTTGGGGGTT CCAGTGCCAT TGCAGGGCCG GCAGGCAACC CAGCCGCTTA CGCCTGGCCA ACCGCCCGTT CCTCCACACA TGGGGCATTC CACGGCGTCG GTGCCTGGTT GTTCTTGATT TTCCATGCCG CCTCCTTTAG CCGCTAAAAT TCATCTACTC ATTTATTCAT TTGCTCATTT ACTCTGGTAG CTGCGCGATG TATTCAGATA GCAGCTCGGT AATGGTCTTG CCTTGGCGTA CCGCGTACAT CTTCAGCTTG GTGTGATCCT CCGCCGGCAA CTGAAAGTTG ACCCGCTTCA TGGCTGGCGT GTCTGCCAGG CTGGCCAACG TTGCAGCCTT GCTGCTGCGT GCGCTCGGAC GGCCGGCACT TAGCGTGTTT GTGCTTTTGC TCATTTTCTC TTTACCTCAT TAACTCAAAT GAGTTTTGAT TTAATTTCAG CGGCCAGCGC CTGGACCTCG CGGGCAGCGT CGCCCTCGGG TTCTGATTCA AGAACGGTTG TGCCGGCGGC GGCAGTGCCT GGGTAGCTCA CGCGCTGCGT GATACGGGAC TCAAGAATGG GCAGCTCGTA CCCGGCCAGC GCCTCGGCAA CCTCACCGCC GATGCGCGTG CCTTTGATCG CCCGCGACAC GACAAAGGCC GCTTGTAGCC TTCCATCCGT GACCTCAATG CGCTGCTTAA CCAGCTCCAC CAGGTCGGCG GTGGCCCATA TGTCGTAAGG GCTTGGCTGC ACCGGAATCA GCACGAAGTC GGCTGCCTTG ATCGCGGACA CAGCCAAGTC CGCCGCCTGG GGCGCTCCGT CGATCACTAC GAAGTCGCGC CGGCCGATGG CCTTCACGTC GCGGTCAATC GTCGGGCGGT CGATGCCGAC AACGGTTAGC GGTTGATCTT CCCGCACGGC CGCCCAATCG CGGGCACTGC CCTGGGGATC GGAATCGACT AACAGAACAT CGGCCCCGGC GAGTTGCAGG GCGCGGGCTA GATGGGTTGC GATGGTCGTC TTGCCTGACC CGCCTTTCTG GTTAAGTACA GCGATAACCT TCATGCGTTC CCCTTGCGTA TTTGTTTATT TACTCATCGC ATCATATACG CAGCGACCGC ATGACGCAAG CTGTTTTACT CAAATACACA TCACCTTTTT AGACGGCGGC GCTCGGTTTC TTCAGCGGCC AAGCTGGCCG GCCAGGCCGC CAGCTTGGCA TCAGACAAAC CGGCCAGGAT TTCATGCAGC CGCACGGTTG AGACGTGCGC GGGCGGCTCG AACACGTACC CGGCCGCGAT CATCTCCGCC TCGATCTCTT CGGTAATGAA AAACGGTTCG TCCTGGCCGT CCTGGTGCGG TTTCATGCTT GTTCCTCTTG GCGTTCATTC TCGGCGGCCG CCAGGGCGTC GGCCTCGGTC AATGCGTCCT CACGGAAGGC ACCGCGCCGC CTGGCCTCGG TGGGCGTCAC TTCCTCGCTG CGCTCAAGTG CGCGGTACAG GGTCGAGCGA TGCACGCCAA GCAGTGCAGC CGCCTCTTTC ACGGTGCGGC CTTCCTGGTC GATCAGCTCG CGGGCGTGCG CGATCTGTGC CGGGGTGAGG GTAGGGCGGG GGCCAAACTT CACGCCTCGG GCCTTGGCGG CCTCGCGCCC GCTCCGGGTG CGGTCGATGA TTAGGGAACG CTCGAACTCG GCAATGCCGG CGAACACGGT CAACACCATG CGGCCGGCCG GCGTGGTGGT GTCGGCCCAC GGCTCTGCCA GGCTACGCAG GCCCGCGCCG GCCTCCTGGA TGCGCTCGGC AATGTCCAGT AGGTCGCGGG TGCTGCGGGC CAGGCGGTCT AGCCTGGTCA CTGTCACAAC GTCGCCAGGG CGTAGGTGGT CAAGCATCCT GGCCAGCTCC GGGCGGTCGC GCCTGGTGCC GGTGATCTTC TCGGAAAACA GCTTGGTGCA GCCGGCCGCG TGCAGTTCGG CCCGTTGGTT GGTCAAGTCC TGGTCGTCGG TGCTGACGCG GGCATAGCCC AGCAGGCCAG CGGCGGCGCT CTTGTTCATG GCGTAATGTC TCCGGTTCTA GTCGCAAGTA TTCTACTTTA TGCGACTAAA ACACGCGACA AGAAAACGCC AGGAAAAGGG CAGGGCGGCA GCCTGTCGCG TAACTTAGGA CTTGTGCGAC ATGTCGTTTT CAGAAGACGG CTGCACTGAA CGTCAGAAGC CGACTGCACT ATAGCAGCGG AGGGGTTGGA TCAAAGTACT TTGATCCCGA GGGGAACCCT GTGGTTGGCA TGCACATACA AATGGACGAA CGGATAAACC TTTTCACGCC CTTTTAAATA TCCGATTATT CTAATAAACG CTCTTTTCTC TTAGGTTTAC CCGCCAATAT ATCCTGTCAA ACACTGATAG TTTAAACTGA AGGCGGGAAA CGACAATCTG ATCCAAGCTC AAGCTGCTCT AGCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCG
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