pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9
100μg liquid plasmid(Instantly):$120
100μg liquid plasmid(Instantly):$120
| name | pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9 | another name | apHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Arabidopsis | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | AtU6 | Replicon | pVS1 |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TGCTCTAGCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTCGACTTG CCTTCCGCAC AATACATCAT TTCTTCTTAG CTTTTTTTCT TCTTCTTCGT TCATACAGTT TTTTTTTGTT TATCAGCTTA CATTTTCTTG AACCGTAGCT TTCGTTTTCT TCTTTTTAAC TTTCCATTCG GAGTTTTTGT ATCTTGTTTC ATAGTTTGTC CCAGGATTAG AATGATTAGG CATCGAACCT TCAAGAATTT GATTGAATAA AACATCTTCA TTCTTAAGAT ATGAAGATAA TCTTCAAAAG GCCCCTGGGA ATCTGAAAGA AGAGAAGCAG GCCCATTTAT ATGGGAAAGA ACAATAGTAT TTCTTATATA GGCCCATTTA AGTTGAAAAC AATCTTCAAA AGTCCCACAT CGCTTAGATA AGAAAACGAA GCTGAGTTTA TATACAGCTA GAGTCGAAGT AGTGATTGCT CTTCCTCCAG GTCATCCTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT TTGCAAAATT TTCCAGATCG ATTTCTTCTT CCTCTGTTCT TCGGCCATCG CTACCTTAGG ACCGTTATAG TTAATTACCC TGTTATCCCT ATTAATTAAG AGCTCAGAAA TCTCAAAATT CCGGCAGAAC AATTTTGAAT CTCGATCCGT AGAAACGAGA CGGTCATTGT TTTAGTTCCA CCACGATTAT ATTTGAAATT TACGTGAGTG TGAGTGAGAC TTGCATAAGA AAATAAAATC TTTAGTTGGG AAAAAATTCA ATAATATAAA TGGGCTTGAG AAGGAAGCGA GGGATAGGCC TTTTTCTAAA ATAGGCCCAT TTAAGCTATT AACAATCTTC AAAAGTACCA CAGCGCTTAG GTAAAGAAAG CAGCTGAGTT TATATATGGT TAGAGACGAA GTAGTGATTG TTGGGTCATA ACGATATCTC GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTTCTAGACC CAGCTTTCTT GTACAAGCTT GCATGCCTGC AGGTCAACAT GGTGGAGCAC GACACACTTG TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATAACATGGT GGAGCACGAC ACACTTGTCT ACTCCAAAAA TATCAAAGAT ACAGTCTCAG AAGACCAAAG GGCAATTGAG ACTTTTCAAC AAAGGGTAAT ATCCGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTTATTG TGAAGATAGT GGAAAAGGAA GGTGGCTCCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG CCATCGTTGA AGATGCCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAC GTTCCAACCA CGTCTTCAAA GCAAGTGGAT TGATGTGATA TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGGA CCTCGACCTC AACACAACAT ATACAAAACA AACGAATCTC AAGCAATCAA GCATTCTACT TCTATTGCAG CAATTTAAAT CATTTCTTTT AAAGCAAAAG CAATTTTCTG AAAATTTTCA CCATTTACGA ACGATACTCG AGTAATCTAG ATGGATTACA AGGACCACGA CGGGGATTAC AAGGACCACG ACATTGATTA CAAGGATGAT GATGACAAGA TGGCTCCGAA GAAGAAGAGG AAGGTTGGCA TCCACGGGGT GCCAGCTGCT GACAAGAAGT ACTCGATCGG CCTCGATATT GGGACTAACT CTGTTGGCTG GGCCGTGATC ACCGACGAGT ACAAGGTGCC CTCAAAGAAG TTCAAGGTCC TGGGCAACAC CGATCGGCAT TCCATCAAGA AGAATCTCAT TGGCGCTCTC CTGTTCGACA GCGGCGAGAC GGCTGAGGCT ACGCGGCTCA AGCGCACCGC CCGCAGGCGG TACACGCGCA GGAAGAATCG CATCTGCTAC CTGCAGGAGA TTTTCTCCAA CGAGATGGCG AAGGTTGACG ATTCTTTCTT CCACAGGCTG GAGGAGTCAT TCCTCGTGGA GGAGGATAAG AAGCACGAGC GGCATCCAAT CTTCGGCAAC ATTGTCGACG AGGTTGCCTA CCACGAGAAG TACCCTACGA TCTACCATCT GCGGAAGAAG CTCGTGGACT CCACAGATAA GGCGGACCTC CGCCTGATCT ACCTCGCTCT GGCCCACATG ATTAAGTTCA GGGGCCATTT CCTGATCGAG GGGGATCTCA ACCCGGACAA TAGCGATGTT GACAAGCTGT TCATCCAGCT CGTGCAGACG TACAACCAGC TCTTCGAGGA GAACCCCATT AATGCGTCAG GCGTCGACGC GAAGGCTATC CTGTCCGCTA GGCTCTCGAA GTCTCGGCGC CTCGAGAACC TGATCGCCCA GCTGCCGGGC GAGAAGAAGA ACGGCCTGTT CGGGAATCTC ATTGCGCTCA GCCTGGGGCT CACGCCCAAC TTCAAGTCGA ATTTCGATCT CGCTGAGGAC GCCAAGCTGC AGCTCTCCAA GGACACATAC GACGATGACC TGGATAACCT CCTGGCCCAG ATCGGCGATC AGTACGCGGA CCTGTTCCTC GCTGCCAAGA ATCTGTCGGA CGCCATCCTC CTGTCTGATA TTCTCAGGGT GAACACCGAG ATTACGAAGG CTCCGCTCTC AGCCTCCATG ATCAAGCGCT ACGACGAGCA CCATCAGGAT CTGACCCTCC TGAAGGCGCT GGTCAGGCAG CAGCTCCCCG AGAAGTACAA GGAGATCTTC TTCGATCAGT CGAAGAACGG CTACGCTGGG TACATTGACG GCGGGGCCTC TCAGGAGGAG TTCTACAAGT TCATCAAGCC GATTCTGGAG AAGATGGACG GCACGGAGGA GCTGCTGGTG AAGCTCAATC GCGAGGACCT CCTGAGGAAG CAGCGGACAT TCGATAACGG CAGCATCCCA CACCAGATTC ATCTCGGGGA GCTGCACGCT ATCCTGAGGA GGCAGGAGGA CTTCTACCCT TTCCTCAAGG ATAACCGCGA GAAGATCGAG AAGATTCTGA CTTTCAGGAT CCCGTACTAC GTCGGCCCAC TCGCTAGGGG CAACTCCCGC TTCGCTTGGA TGACCCGCAA GTCAGAGGAG ACGATCACGC CGTGGAACTT CGAGGAGGTG GTCGACAAGG GCGCTAGCGC TCAGTCGTTC ATCGAGAGGA TGACGAATTT CGACAAGAAC CTGCCAAATG AGAAGGTGCT CCCTAAGCAC TCGCTCCTGT ACGAGTACTT CACAGTCTAC AACGAGCTGA CTAAGGTGAA GTATGTGACC GAGGGCATGA GGAAGCCGGC TTTCCTGTCT GGGGAGCAGA AGAAGGCCAT CGTGGACCTC CTGTTCAAGA CCAACCGGAA GGTCACGGTT AAGCAGCTCA AGGAGGACTA CTTCAAGAAG ATTGAGTGCT TCGATTCGGT CGAGATCTCT GGCGTTGAGG ACCGCTTCAA CGCCTCCCTG GGGACCTACC ACGATCTCCT GAAGATCATT AAGGATAAGG ACTTCCTGGA CAACGAGGAG AATGAGGATA TCCTCGAGGA CATTGTGCTG ACACTCACTC TGTTCGAGGA CCGGGAGATG ATCGAGGAGC GCCTGAAGAC TTACGCCCAT CTCTTCGATG ACAAGGTCAT GAAGCAGCTC AAGAGGAGGA GGTACACCGG CTGGGGGAGG CTGAGCAGGA AGCTCATCAA CGGCATTCGG GACAAGCAGT CCGGGAAGAC GATCCTCGAC TTCCTGAAGA GCGATGGCTT CGCGAACCGC AATTTCATGC AGCTGATTCA CGATGACAGC CTCACATTCA AGGAGGATAT CCAGAAGGCT CAGGTGAGCG GCCAGGGGGA CTCGCTGCAC GAGCATATCG CGAACCTCGC TGGCTCGCCA GCTATCAAGA AGGGGATTCT GCAGACCGTG AAGGTTGTGG ACGAGCTGGT GAAGGTCATG GGCAGGCACA AGCCTGAGAA CATCGTCATT GAGATGGCCC GGGAGAATCA GACCACGCAG AAGGGCCAGA AGAACTCACG CGAGAGGATG AAGAGGATCG AGGAGGGCAT TAAGGAGCTG GGGTCCCAGA TCCTCAAGGA GCACCCGGTG GAGAACACGC AGCTGCAGAA TGAGAAGCTC TACCTGTACT ACCTCCAGAA TGGCCGCGAT ATGTATGTGG ACCAGGAGCT GGATATTAAC AGGCTCAGCG ATTACGACGT CGATCATATC GTTCCACAGT CATTCCTGAA GGATGACTCC ATTGACAACA AGGTCCTCAC CAGGTCGGAC AAGAACCGGG GCAAGTCTGA TAATGTTCCT TCAGAGGAGG TCGTTAAGAA GATGAAGAAC TACTGGCGCC AGCTCCTGAA TGCCAAGCTG ATCACGCAGC GGAAGTTCGA TAACCTCACA AAGGCTGAGA GGGGCGGGCT CTCTGAGCTG GACAAGGCGG GCTTCATCAA GAGGCAGCTG GTCGAGACAC GGCAGATCAC TAAGCACGTT GCGCAGATTC TCGACTCACG GATGAACACT AAGTACGATG AGAATGACAA GCTGATCCGC GAGGTGAAGG TCATCACCCT GAAGTCAAAG CTCGTCTCCG ACTTCAGGAA GGATTTCCAG TTCTACAAGG TTCGGGAGAT CAACAATTAC CACCATGCCC ATGACGCGTA CCTGAACGCG GTGGTCGGCA CAGCTCTGAT CAAGAAGTAC CCAAAGCTCG AGAGCGAGTT CGTGTACGGG GACTACAAGG TTTACGATGT GAGGAAGATG ATCGCCAAGT CGGAGCAGGA GATTGGCAAG GCTACCGCCA AGTACTTCTT CTACTCTAAC ATTATGAATT TCTTCAAGAC AGAGATCACT CTGGCCAATG GCGAGATCCG GAAGCGCCCC CTCATCGAGA CGAACGGCGA GACGGGGGAG ATCGTGTGGG ACAAGGGCAG GGATTTCGCG ACCGTCAGGA AGGTTCTCTC CATGCCACAA GTGAATATCG TCAAGAAGAC AGAGGTCCAG ACTGGCGGGT TCTCTAAGGA GTCAATTCTG CCTAAGCGGA ACAGCGACAA GCTCATCGCC CGCAAGAAGG ACTGGGATCC GAAGAAGTAC GGCGGGTTCG ACAGCCCCAC TGTGGCCTAC TCGGTCCTGG TTGTGGCGAA GGTTGAGAAG GGCAAGTCCA AGAAGCTCAA GAGCGTGAAG GAGCTGCTGG GGATCACGAT TATGGAGCGC TCCAGCTTCG AGAAGAACCC GATCGATTTC CTGGAGGCGA AGGGCTACAA GGAGGTGAAG AAGGACCTGA TCATTAAGCT CCCCAAGTAC TCACTCTTCG AGCTGGAGAA CGGCAGGAAG CGGATGCTGG CTTCCGCTGG CGAGCTGCAG AAGGGGAACG AGCTGGCTCT GCCGTCCAAG TATGTGAACT TCCTCTACCT GGCCTCCCAC TACGAGAAGC TCAAGGGCAG CCCCGAGGAC AACGAGCAGA AGCAGCTGTT CGTCGAGCAG CACAAGCATT ACCTCGACGA GATCATTGAG CAGATTTCCG AGTTCTCCAA GCGCGTGATC CTGGCCGACG CGAATCTGGA TAAGGTCCTC TCCGCGTACA ACAAGCACCG CGACAAGCCA ATCAGGGAGC AGGCTGAGAA TATCATTCAT CTCTTCACCC TGACGAACCT CGGCGCCCCT GCTGCTTTCA AGTACTTCGA CACAACTATC GATCGCAAGA GGTACACAAG CACTAAGGAG GTCCTGGACG CGACCCTCAT CCACCAGTCG ATTACCGGCC TCTACGAGAC GCGCATCGAC CTGTCTCAGC TCGGGGGCGA CAAGCGGCCA GCGGCGACGA AGAAGGCGGG GCAGGCGAAG AAGAAGAAGT GAGCTCAGAG CTTTCGTTCG TATCATCGGT TTCGACAACG TTCGTCAAGT TCAATGCATC AGTTTCATTG CGCACACACC AGAATCCTAC TGAGTTTGAG TATTATGGCA TTGGGAAAAC TGTTTTTCTT GTACCATTTG TTGTGCTTGT AATTTACTGT GTTTTTTATT CGGTTTTCGC TATCGAACTG TGAAATGGAA ATGGATGGAG AAGAGTTAAT GAATGATATG GTCCTTTTGT TCATTCTCAA ATTAATATTA TTTGTTTTTT CTCTTATTTG TTGTGTGTTG AATTTGAAAT TATAAGAGAT ATGCAAACAT TTTGTTTTGA GTAAAAATGT GTCAAATCGT GGCCTCTAAT GACCGAAGTT AATATGAGGA GTAAAACACT TGTAGTTGTA CCATTATGCT TATTCACTAG GCAACAAATA TATTTTCAGA CCTAGAAAAG CTGCAAATGT TACTGAATAC AAGTATGTCC TCTTGTGTTT TAGACATTTA TGAACTTTCC TTTATGTAAT TTTCCAGAAT CCTTGTCAGA TTCTAATCAT TGCTTTATAA TTATAGTTAT ACTCATGGAT TTGTAGTTGA GTATGAAAAT ATTTTTTAAT GCATTTTATG ACTTGCCAAT TGATTGACAA CGAATTCGTA ATCATGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GCTAGAGCAG CTTGCCAACA TGGTGGAGCA CGACACTCTC GTCTACTCCA AGAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCTAT TGAGACTTTT CAACAAAGGG TAATATCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCAAAAGGA CAGTAGAAAA GGAAGGTGGC ACCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GAACATGGTG GAGCACGACA CTCTCGTCTA CTCCAAGAAT ATCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGGTAATA TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCAA AAGGACAGTA GAAAAGGAAG GTGGCACCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCGTTCAA GATGCCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGATAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGCTGAAAT CACCAGTCTC TCTCTACAAA TCTATCTCTC TCGAGCTTTC GCAGATCCGG GGGGCAATGA GATATGAAAA AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT TTAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTTCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC GCTGTTCTAC AACCGGTCGC GGAGGCTATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCACGACTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA AAGAAATAGA GTAGATGCCG ACCGGGATCT GTCGATCGAC AAGCTCGAGT TTCTCCATAA TAATGTGTGA GTAGTTCCCA GATAAGGGAA TTAGGGTTCC TATAGGGTTT CGCTCATGTG TTGAGCATAT AAGAAACCCT TAGTATGTAT TTGTATTTGT AAAATACTTC TATCAATAAA ATTTCTAATT CCTAAAACCA AAATCCAGTA CTAAAATCCA GATCCCCCGA ATTAATTCGG CGTTAATTCA GTACATTAAA AACGTCCGCA ATGTGTTATT AAGTTGTCTA AGCGTCAATT TGTTTACACC ACAATATATC CTGCCACCAG CCAGCCAACA GCTCCCCGAC CGGCAGCTCG GCACAAAATC ACCACTCGAT ACAGGCAGCC CATCAGTCCG GGACGGCGTC AGCGGGAGAG CCGTTGTAAG GCGGCAGACT TTGCTCATGT TACCGATGCT ATTCGGAAGA ACGGCAACTA AGCTGCCGGG TTTGAAACAC GGATGATCTC GCGGAGGGTA GCATGTTGAT TGTAACGATG ACAGAGCGTT GCTGCCTGTG ATCACCGCGG TTTCAAAATC GGCTCCGTCG ATACTATGTT ATACGCCAAC TTTGAAAACA ACTTTGAAAA AGCTGTTTTC TGGTATTTAA GGTTTTAGAA TGCAAGGAAC AGTGAATTGG AGTTCGTCTT GTTATAATTA GCTTCTTGGG GTATCTTTAA ATACTGTAGA AAAGAGGAAG GAAATAATAA ATGGCTAAAA TGAGAATATC ACCGGAATTG AAAAAACTGA TCGAAAAATA CCGCTGCGTA AAAGATACGG AAGGAATGTC TCCTGCTAAG GTATATAAGC TGGTGGGAGA AAATGAAAAC CTATATTTAA AAATGACGGA CAGCCGGTAT AAAGGGACCA CCTATGATGT GGAACGGGAA AAGGACATGA TGCTATGGCT GGAAGGAAAG CTGCCTGTTC CAAAGGTCCT GCACTTTGAA CGGCATGATG GCTGGAGCAA TCTGCTCATG AGTGAGGCCG ATGGCGTCCT TTGCTCGGAA GAGTATGAAG ATGAACAAAG CCCTGAAAAG ATTATCGAGC TGTATGCGGA GTGCATCAGG CTCTTTCACT CCATCGACAT ATCGGATTGT CCCTATACGA ATAGCTTAGA CAGCCGCTTA GCCGAATTGG ATTACTTACT GAATAACGAT CTGGCCGATG TGGATTGCGA AAACTGGGAA GAAGACACTC CATTTAAAGA TCCGCGCGAG CTGTATGATT TTTTAAAGAC GGAAAAGCCC GAAGAGGAAC TTGTCTTTTC CCACGGCGAC CTGGGAGACA GCAACATCTT TGTGAAAGAT GGCAAAGTAA GTGGCTTTAT TGATCTTGGG AGAAGCGGCA GGGCGGACAA GTGGTATGAC ATTGCCTTCT GCGTCCGGTC GATCAGGGAG GATATCGGGG AAGAACAGTA TGTCGAGCTA TTTTTTGACT TACTGGGGAT CAAGCCTGAT TGGGAGAAAA TAAAATATTA TATTTTACTG GATGAATTGT TTTAGTACCT AGAATGCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGGGT GCCTTGATGT GGGCGCCGGC GGTCGAGTGG CGACGGCGCG GCTTGTCCGC GCCCTGGTAG ATTGCCTGGC CGTAGGCCAG CCATTTTTGA GCGGCCAGCG GCCGCGATAG GCCGACGCGA AGCGGCGGGG CGTAGGGAGC GCAGCGACCG AAGGGTAGGC GCTTTTTGCA GCTCTTCGGC TGTGCGCTGG CCAGACAGTT ATGCACAGGC CAGGCGGGTT TTAAGAGTTT TAATAAGTTT TAAAGAGTTT TAGGCGGAAA AATCGCCTTT TTTCTCTTTT ATATCAGTCA CTTACATGTG TGACCGGTTC CCAATGTACG GCTTTGGGTT CCCAATGTAC GGGTTCCGGT TCCCAATGTA CGGCTTTGGG TTCCCAATGT ACGTGCTATC CACAGGAAAC AGACCTTTTC GACCTTTTTC CCCTGCTAGG GCAATTTGCC CTAGCATCTG CTCCGTACAT TAGGAACCGG CGGATGCTTC GCCCTCGATC AGGTTGCGGT AGCGCATGAC TAGGATCGGG CCAGCCTGCC CCGCCTCCTC CTTCAAATCG TACTCCGGCA GGTCATTTGA CCCGATCAGC TTGCGCACGG TGAAACAGAA CTTCTTGAAC TCTCCGGCGC TGCCACTGCG TTCGTAGATC GTCTTGAACA ACCATCTGGC TTCTGCCTTG CCTGCGGCGC GGCGTGCCAG GCGGTAGAGA AAACGGCCGA TGCCGGGATC GATCAAAAAG TAATCGGGGT GAACCGTCAG CACGTCCGGG TTCTTGCCTT CTGTGATCTC GCGGTACATC CAATCAGCTA GCTCGATCTC GATGTACTCC GGCCGCCCGG TTTCGCTCTT TACGATCTTG TAGCGGCTAA TCAAGGCTTC ACCCTCGGAT ACCGTCACCA GGCGGCCGTT CTTGGCCTTC TTCGTACGCT GCATGGCAAC GTGCGTGGTG TTTAACCGAA TGCAGGTTTC TACCAGGTCG TCTTTCTGCT TTCCGCCATC GGCTCGCCGG CAGAACTTGA GTACGTCCGC AACGTGTGGA CGGAACACGC GGCCGGGCTT GTCTCCCTTC CCTTCCCGGT ATCGGTTCAT GGATTCGGTT AGATGGGAAA CCGCCATCAG TACCAGGTCG TAATCCCACA CACTGGCCAT GCCGGCCGGC CCTGCGGAAA CCTCTACGTG CCCGTCTGGA AGCTCGTAGC GGATCACCTC GCCAGCTCGT CGGTCACGCT TCGACAGACG GAAAACGGCC ACGTCCATGA TGCTGCGACT ATCGCGGGTG CCCACGTCAT AGAGCATCGG AACGAAAAAA TCTGGTTGCT CGTCGCCCTT GGGCGGCTTC CTAATCGACG GCGCACCGGC TGCCGGCGGT TGCCGGGATT CTTTGCGGAT TCGATCAGCG GCCGCTTGCC ACGATTCACC GGGGCGTGCT TCTGCCTCGA TGCGTTGCCG CTGGGCGGCC TGCGCGGCCT TCAACTTCTC CACCAGGTCA TCACCCAGCG CCGCGCCGAT TTGTACCGGG CCGGATGGTT TGCGACCGCT CACGCCGATT CCTCGGGCTT GGGGGTTCCA GTGCCATTGC AGGGCCGGCA GGCAACCCAG CCGCTTACGC CTGGCCAACC GCCCGTTCCT CCACACATGG GGCATTCCAC GGCGTCGGTG CCTGGTTGTT CTTGATTTTC CATGCCGCCT CCTTTAGCCG CTAAAATTCA TCTACTCATT TATTCATTTG CTCATTTACT CTGGTAGCTG CGCGATGTAT TCAGATAGCA GCTCGGTAAT GGTCTTGCCT TGGCGTACCG CGTACATCTT CAGCTTGGTG TGATCCTCCG CCGGCAACTG AAAGTTGACC CGCTTCATGG CTGGCGTGTC TGCCAGGCTG GCCAACGTTG CAGCCTTGCT GCTGCGTGCG CTCGGACGGC CGGCACTTAG CGTGTTTGTG CTTTTGCTCA TTTTCTCTTT ACCTCATTAA CTCAAATGAG TTTTGATTTA ATTTCAGCGG CCAGCGCCTG GACCTCGCGG GCAGCGTCGC CCTCGGGTTC TGATTCAAGA ACGGTTGTGC CGGCGGCGGC AGTGCCTGGG TAGCTCACGC GCTGCGTGAT ACGGGACTCA AGAATGGGCA GCTCGTACCC GGCCAGCGCC TCGGCAACCT CACCGCCGAT GCGCGTGCCT TTGATCGCCC GCGACACGAC AAAGGCCGCT TGTAGCCTTC CATCCGTGAC CTCAATGCGC TGCTTAACCA GCTCCACCAG GTCGGCGGTG GCCCATATGT CGTAAGGGCT TGGCTGCACC GGAATCAGCA CGAAGTCGGC TGCCTTGATC GCGGACACAG CCAAGTCCGC CGCCTGGGGC GCTCCGTCGA TCACTACGAA GTCGCGCCGG CCGATGGCCT TCACGTCGCG GTCAATCGTC GGGCGGTCGA TGCCGACAAC GGTTAGCGGT TGATCTTCCC GCACGGCCGC CCAATCGCGG GCACTGCCCT GGGGATCGGA ATCGACTAAC AGAACATCGG CCCCGGCGAG TTGCAGGGCG CGGGCTAGAT GGGTTGCGAT GGTCGTCTTG CCTGACCCGC CTTTCTGGTT AAGTACAGCG ATAACCTTCA TGCGTTCCCC TTGCGTATTT GTTTATTTAC TCATCGCATC ATATACGCAG CGACCGCATG ACGCAAGCTG TTTTACTCAA ATACACATCA CCTTTTTAGA CGGCGGCGCT CGGTTTCTTC AGCGGCCAAG CTGGCCGGCC AGGCCGCCAG CTTGGCATCA GACAAACCGG CCAGGATTTC ATGCAGCCGC ACGGTTGAGA CGTGCGCGGG CGGCTCGAAC ACGTACCCGG CCGCGATCAT CTCCGCCTCG ATCTCTTCGG TAATGAAAAA CGGTTCGTCC TGGCCGTCCT GGTGCGGTTT CATGCTTGTT CCTCTTGGCG TTCATTCTCG GCGGCCGCCA GGGCGTCGGC CTCGGTCAAT GCGTCCTCAC GGAAGGCACC GCGCCGCCTG GCCTCGGTGG GCGTCACTTC CTCGCTGCGC TCAAGTGCGC GGTACAGGGT CGAGCGATGC ACGCCAAGCA GTGCAGCCGC CTCTTTCACG GTGCGGCCTT CCTGGTCGAT CAGCTCGCGG GCGTGCGCGA TCTGTGCCGG GGTGAGGGTA GGGCGGGGGC CAAACTTCAC GCCTCGGGCC TTGGCGGCCT CGCGCCCGCT CCGGGTGCGG TCGATGATTA GGGAACGCTC GAACTCGGCA ATGCCGGCGA ACACGGTCAA CACCATGCGG CCGGCCGGCG TGGTGGTGTC GGCCCACGGC TCTGCCAGGC TACGCAGGCC CGCGCCGGCC TCCTGGATGC GCTCGGCAAT GTCCAGTAGG TCGCGGGTGC TGCGGGCCAG GCGGTCTAGC CTGGTCACTG TCACAACGTC GCCAGGGCGT AGGTGGTCAA GCATCCTGGC CAGCTCCGGG CGGTCGCGCC TGGTGCCGGT GATCTTCTCG GAAAACAGCT TGGTGCAGCC GGCCGCGTGC AGTTCGGCCC GTTGGTTGGT CAAGTCCTGG TCGTCGGTGC TGACGCGGGC ATAGCCCAGC AGGCCAGCGG CGGCGCTCTT GTTCATGGCG TAATGTCTCC GGTTCTAGTC GCAAGTATTC TACTTTATGC GACTAAAACA CGCGACAAGA AAACGCCAGG AAAAGGGCAG GGCGGCAGCC TGTCGCGTAA CTTAGGACTT GTGCGACATG TCGTTTTCAG AAGACGGCTG CACTGAACGT CAGAAGCCGA CTGCACTATA GCAGCGGAGG GGTTGGATCA AAGTACTTTG ATCCCGAGGG GAACCCTGTG GTTGGCATGC ACATACAAAT GGACGAACGG ATAAACCTTT TCACGCCCTT TTAAATATCC GATTATTCTA A