pBUN6I11-AtU6-FPA-sgRNA1-35S-Cas9
$99.00
In stock
SKU
PL56269
100μg liquid plasmid(Instantly):$120
| name | pBUN6I11-AtU6-FPA-sgRNA1-35S-Cas9 | another name | apBUN6I11-AtU6-FPA-sgRNA1-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | UBI,OsU3 | Replicon | pSa |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TAAGCTCACG TGACGGAATT AAGCTTCGTT GAACAACGGA AACTCGACTT GCCTTCCGCA CAATACATCA TTTCTTCTTA GCTTTTTTTC TTCTTCTTCG TTCATACAGT TTTTTTTTGT TTATCAGCTT ACATTTTCTT GAACCGTAGC TTTCGTTTTC TTCTTTTTAA CTTTCCATTC GGAGTTTTTG TATCTTGTTT CATAGTTTGT CCCAGGATTA GAATGATTAG GCATCGAACC TTCAAGAATT TGATTGAATA AAACATCTTC ATTCTTAAGA TATGAAGATA ATCTTCAAAA GGCCCCTGGG AATCTGAAAG AAGAGAAGCA GGCCCATTTA TATGGGAAAG AACAATAGTA TTTCTTATAT AGGCCCATTT AAGTTGAAAA CAATCTTCAA AAGTCCCACA TCGCTTAGAT AAGAAAACGA AGCTGAGTTT ATATACAGCT AGAGTCGAAG TAGTGATTGT CAAAATCCTA CCTACAGAGG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTGTCCCTTC GAAGGGCAAT TCTGCAGATA TCCATCACAC TGGCGGCCGC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCCT GCAGTGCAGC GTGACCCGGT CGTGCCCCTC TCTAGAGATA ATGAGCATTG CATGTCTAAG TTATAAAAAA TTACCACATA TTTTTTTTGT CACACTTGTT TGAAGTGCAG TTTATCTATC TTTATACATA TATTTAAACT TTACTCTACG AATAATATAA TCTATAGTAC TACAATAATA TCAGTGTTTT AGAGAATCAT ATAAATGAAC AGTTAGACAT GGTCTAAAGG ACAATTGAGT ATTTTGACAA CAGGACTCTA CAGTTTTATC TTTTTAGTGT GCATGTGTTC TCCTTTTTTT TTGCAAATAG CTTCACCTAT ATAATACTTC ATCCATTTTA TTAGTACATC CATTTAGGGT TTAGGGTTAA TGGTTTTTAT AGACTAATTT TTTTAGTACA TCTATTTTAT TCTATTTTAG CCTCTAAATT AAGAAAACTA AAACTCTATT TTAGTTTTTT TATTTAATAA TTTAGATATA AAATAGAATA AAATAAAGTG ACTAAAAATT AAACAAATAC CCTTTAAGAA ATTAAAAAAA CTAAGGAAAC ATTTTTCTTG TTTCGAGTAG ATAATGCCAG CCTGTTAAAC GCCGTCGACG AGTCTAACGG ACACCAACCA GCGAACCAGC AGCGTCGCGT CGGGCCAAGC GAAGCAGACG GCACGGCATC TCTGTCGCTG CCTCTGGACC CCTCTCGAGA GTTCCGCTCC ACCGTTGGAC TTGCTCCGCT GTCGGCATCC AGAAATGCGT GGCGGAGCGG CAGACGTGAG CCGGCACGGC AGGCGGCCTC CTCCTCCTCT CACGGCACGG CAGCTACGGG GGATTCCTTT CCCACCGCTC CTTCGCTTTC CCTTCCTCGC CCGCCGTAAT AAATAGACAC CCCCTCCACA CCCTCTTTCC CCAACCTCGT GTTGTTCGGA GCGCACACAC ACACAACCAG ATCTCCCCCA AATCCACCCG TCGGCACCTC CGCTTCAAGG TACGCCGCTC GTCCTCCCCC CCCCCCCCTC TCTACCTTCT CTAGATCGGC GTTCCGGTCC ATGGTTAGGG CCCGGTAGTT CTACTTCTGT TCATGTTTGT GTTAGATCCG TGTTTGTGTT AGATCCGTGC TGCTAGCGTT CGTACACGGA TGCGACCTGT ACGTCAGACA CGTTCTGATT GCTAACTTGC CAGTGTTTCT CTTTGGGGAA TCCTGGGATG GCTCTAGCCG TTCCGCAGAC GGGATCGATT TCATGATTTT TTTTGTTTCG TTGCATAGGG TTTGGTTTGC CCTTTTCCTT TATTTCAATA TATGCCGTGC ACTTGTTTGT CGGGTCATCT TTTCATGCTT TTTTTTGTCT TGGTTGTGAT GATGTGGTCT GGTTGGGCGG TCGTTCTAGA TCGGAGTAGA ATTCTGTTTC AAACTACCTG GTGGATTTAT TAATTTTGGA TCTGTATGTG TGTGCCATAC ATATTCATAG TTACGAATTG AAGATGATGG ATGGAAATAT CGATCTAGGA TAGGTATACA TGTTGATGCG GGTTTTACTG ATGCATATAC AGAGATGCTT TTTGTTCGCT TGGTTGTGAT GATGTGGTGT GGTTGGGCGG TCGTTCATTC GTTCTAGATC GGAGTAGAAT ACTGTTTCAA ACTACCTGGT GTATTTATTA ATTTTGGAAC TGTATGTGTG TGTCATACAT CTTCATAGTT ACGAGTTTAA GATGGATGGA AATATCGATC TAGGATAGGT ATACATGTTG ATGTGGGTTT TACTGATGCA TATACATGAT GGCATATGCA GCATCTATTC ATATGCTCTA ACCTTGAGTA CCTATCTATT ATAATAAACA AGTATGTTTT ATAATTATTT TGATCTTGAT ATACTTGGAT GATGGCATAT GCAGCAGCTA TATGTGGATT TTTTTAGCCC TGCCTTCATA CGCTATTTAT TTGCTTGGTA CTGTTTCTTT TGTCGATGCT CACCCTGTTG TTTGGTGTTA CTTCTGCAGC CCGGGGGATC CCCAATACTT GTATGGCCGC GGCCGCTCTA GATGGATTAC AAGGACCACG ACGGGGATTA CAAGGACCAC GACATTGATT ACAAGGATGA TGATGACAAG ATGGCTCCGA AGAAGAAGAG GAAGGTTGGC ATCCACGGGG TGCCAGCTGC TGACAAGAAG TACTCGATCG GCCTCGCCAT TGGGACTAAC TCTGTTGGCT GGGCCGTGAT CACCGACGAG TACAAGGTGC CCTCAAAGAA GTTCAAGGTC CTGGGCAACA CCGATCGGCA TTCCATCAAG AAGAATCTCA TTGGCGCTCT CCTGTTCGAC AGCGGCGAGA CGGCTGAGGC TACGCGGCTC AAGCGCACCG CCCGCAGGCG GTACACGCGC AGGAAGAATC GCATCTGCTA CCTGCAGGAG ATTTTCTCCA ACGAGATGGC GAAGGTTGAC GATTCTTTCT TCCACAGGCT GGAGGAGTCA TTCCTCGTGG AGGAGGATAA GAAGCACGAG CGGCATCCAA TCTTCGGCAA CATTGTCGAC GAGGTTGCCT ACCACGAGAA GTACCCTACG ATCTACCATC TGCGGAAGAA GCTCGTGGAC TCCACAGATA AGGCGGACCT CCGCCTGATC TACCTCGCTC TGGCCCACAT GATTAAGTTC AGGGGCCATT TCCTGATCGA GGGGGATCTC AACCCGGACA ATAGCGATGT TGACAAGCTG TTCATCCAGC TCGTGCAGAC GTACAACCAG CTCTTCGAGG AGAACCCCAT TAATGCGTCA GGCGTCGACG CGAAGGCTAT CCTGTCCGCT AGGCTCTCGA AGTCTCGGCG CCTCGAGAAC CTGATCGCCC AGCTGCCGGG CGAGAAGAAG AACGGCCTGT TCGGGAATCT CATTGCGCTC AGCCTGGGGC TCACGCCCAA CTTCAAGTCG AATTTCGATC TCGCTGAGGA CGCCAAGCTG CAGCTCTCCA AGGACACATA CGACGATGAC CTGGATAACC TCCTGGCCCA GATCGGCGAT CAGTACGCGG ACCTGTTCCT CGCTGCCAAG AATCTGTCGG ACGCCATCCT CCTGTCTGAT ATTCTCAGGG TGAACACCGA GATTACGAAG GCTCCGCTCT CAGCCTCCAT GATCAAGCGC TACGACGAGC ACCATCAGGA TCTGACCCTC CTGAAGGCGC TGGTCAGGCA GCAGCTCCCC GAGAAGTACA AGGAGATCTT CTTCGATCAG TCGAAGAACG GCTACGCTGG GTACATTGAC GGCGGGGCCT CTCAGGAGGA GTTCTACAAG TTCATCAAGC CGATTCTGGA GAAGATGGAC GGCACGGAGG AGCTGCTGGT GAAGCTCAAT CGCGAGGACC TCCTGAGGAA GCAGCGGACA TTCGATAACG GCAGCATCCC ACACCAGATT CATCTCGGGG AGCTGCACGC TATCCTGAGG AGGCAGGAGG ACTTCTACCC TTTCCTCAAG GATAACCGCG AGAAGATCGA GAAGATTCTG ACTTTCAGGA TCCCGTACTA CGTCGGCCCA CTCGCTAGGG GCAACTCCCG CTTCGCTTGG ATGACCCGCA AGTCAGAGGA GACGATCACG CCGTGGAACT TCGAGGAGGT GGTCGACAAG GGCGCTAGCG CTCAGTCGTT CATCGAGAGG ATGACGAATT TCGACAAGAA CCTGCCAAAT GAGAAGGTGC TCCCTAAGCA CTCGCTCCTG TACGAGTACT TCACAGTCTA CAACGAGCTG ACTAAGGTGA AGTATGTGAC CGAGGGCATG AGGAAGCCGG CTTTCCTGTC TGGGGAGCAG AAGAAGGCCA TCGTGGACCT CCTGTTCAAG ACCAACCGGA AGGTCACGGT TAAGCAGCTC AAGGAGGACT ACTTCAAGAA GATTGAGTGC TTCGATTCGG TCGAGATCTC TGGCGTTGAG GACCGCTTCA ACGCCTCCCT GGGGACCTAC CACGATCTCC TGAAGATCAT TAAGGATAAG GACTTCCTGG ACAACGAGGA GAATGAGGAT ATCCTCGAGG ACATTGTGCT GACACTCACT CTGTTCGAGG ACCGGGAGAT GATCGAGGAG CGCCTGAAGA CTTACGCCCA TCTCTTCGAT GACAAGGTCA TGAAGCAGCT CAAGAGGAGG AGGTACACCG GCTGGGGGAG GCTGAGCAGG AAGCTCATCA ACGGCATTCG GGACAAGCAG TCCGGGAAGA CGATCCTCGA CTTCCTGAAG AGCGATGGCT TCGCGAACCG CAATTTCATG CAGCTGATTC ACGATGACAG CCTCACATTC AAGGAGGATA TCCAGAAGGC TCAGGTGAGC GGCCAGGGGG ACTCGCTGCA CGAGCATATC GCGAACCTCG CTGGCTCGCC AGCTATCAAG AAGGGGATTC TGCAGACCGT GAAGGTTGTG GACGAGCTGG TGAAGGTCAT GGGCAGGCAC AAGCCTGAGA ACATCGTCAT TGAGATGGCC CGGGAGAATC AGACCACGCA GAAGGGCCAG AAGAACTCAC GCGAGAGGAT GAAGAGGATC GAGGAGGGCA TTAAGGAGCT GGGGTCCCAG ATCCTCAAGG AGCACCCGGT GGAGAACACG CAGCTGCAGA ATGAGAAGCT CTACCTGTAC TACCTCCAGA ATGGCCGCGA TATGTATGTG GACCAGGAGC TGGATATTAA CAGGCTCAGC GATTACGACG TCGATGCCAT CGTTCCACAG TCATTCCTGA AGGATGACTC CATTGACAAC AAGGTCCTCA CCAGGTCGGA CAAGAACCGG GGCAAGTCTG ATAATGTTCC TTCAGAGGAG GTCGTTAAGA AGATGAAGAA CTACTGGCGC CAGCTCCTGA ATGCCAAGCT GATCACGCAG CGGAAGTTCG ATAACCTCAC AAAGGCTGAG AGGGGCGGGC TCTCTGAGCT GGACAAGGCG GGCTTCATCA AGAGGCAGCT GGTCGAGACA CGGCAGATCA CTAAGCACGT TGCGCAGATT CTCGACTCAC GGATGAACAC TAAGTACGAT GAGAATGACA AGCTGATCCG CGAGGTGAAG GTCATCACCC TGAAGTCAAA GCTCGTCTCC GACTTCAGGA AGGATTTCCA GTTCTACAAG GTTCGGGAGA TCAACAATTA CCACCATGCC CATGACGCGT ACCTGAACGC GGTGGTCGGC ACAGCTCTGA TCAAGAAGTA CCCAAAGCTC GAGAGCGAGT TCGTGTACGG GGACTACAAG GTTTACGATG TGAGGAAGAT GATCGCCAAG TCGGAGCAGG AGATTGGCAA GGCTACCGCC AAGTACTTCT TCTACTCTAA CATTATGAAT TTCTTCAAGA CAGAGATCAC TCTGGCCAAT GGCGAGATCC GGAAGCGCCC CCTCATCGAG ACGAACGGCG AGACGGGGGA GATCGTGTGG GACAAGGGCA GGGATTTCGC GACCGTCAGG AAGGTTCTCT CCATGCCACA AGTGAATATC GTCAAGAAGA CAGAGGTCCA GACTGGCGGG TTCTCTAAGG AGTCAATTCT GCCTAAGCGG AACAGCGACA AGCTCATCGC CCGCAAGAAG GACTGGGATC CGAAGAAGTA CGGCGGGTTC GACAGCCCCA CTGTGGCCTA CTCGGTCCTG GTTGTGGCGA AGGTTGAGAA GGGCAAGTCC AAGAAGCTCA AGAGCGTGAA GGAGCTGCTG GGGATCACGA TTATGGAGCG CTCCAGCTTC GAGAAGAACC CGATCGATTT CCTGGAGGCG AAGGGCTACA AGGAGGTGAA GAAGGACCTG ATCATTAAGC TCCCCAAGTA CTCACTCTTC GAGCTGGAGA ACGGCAGGAA GCGGATGCTG GCTTCCGCTG GCGAGCTGCA GAAGGGGAAC GAGCTGGCTC TGCCGTCCAA GTATGTGAAC TTCCTCTACC TGGCCTCCCA CTACGAGAAG CTCAAGGGCA GCCCCGAGGA CAACGAGCAG AAGCAGCTGT TCGTCGAGCA GCACAAGCAT TACCTCGACG AGATCATTGA GCAGATTTCC GAGTTCTCCA AGCGCGTGAT CCTGGCCGAC GCGAATCTGG ATAAGGTCCT CTCCGCGTAC AACAAGCACC GCGACAAGCC AATCAGGGAG CAGGCTGAGA ATATCATTCA TCTCTTCACC CTGACGAACC TCGGCGCCCC TGCTGCTTTC AAGTACTTCG ACACAACTAT CGATCGCAAG AGGTACACAA GCACTAAGGA GGTCCTGGAC GCGACCCTCA TCCACCAGTC GATTACCGGC CTCTACGAGA CGCGCATCGA CCTGTCTCAG CTCGGGGGCG ACAAGCGGCC AGCGGCGACG AAGAAGGCGG GGCAGGCGAA GAAGAAGAAG TCCGGGTCCT CGGGGTCGCC AAAGAAGAAG CGGAAGGTCG GCTCAAGCGG GTCACCAAAG AAGAAGCGGA AGGTCGGCTC GATGGACGCT AAGTCCCTCA CCGCTTGGAG CCGCACCCTG GTGACGTTCA AGGACGTGTT CGTCGATTTC ACAAGGGAGG AGTGGAAGCT CCTGGACACT GCTCAGCAGA TCGTTTACCG GAACGTGATG CTGGAGAACT ACAAGAACCT CGTGTCCCTC GGCTACCAGC TGACGAAGCC GGATGTTATT CTCCGCCTGG AGAAGGGCGA GGAGCCCGGG TCCCCTAAGA AGAAGCGGAA GGTCGGCTCC TACCCATACG ATGTCCCTGA CTACGCCTCA TGAGCTCAGG CCTCCATACA AGTATTGGGG ATCCGAATTT CCCCGATCGT TCAAACATTT GGCAATAAAG TTTCTTAAGA TTGAATCCTG TTGCCGGTCT TGCGATGATT ATCATATAAT TTCTGTTGAA TTACGTTAAG CATGTAATAA TTAACATGTA ATGCATGACG TTATTTATGA GATGGGTTTT TATGATTAGA GTCCCGCAAT TATACATTTA ATACGCGATA GAAAACAAAA TATAGCGCGC AAACTAGGAT AAATTATCGC GCGCGGTGTC ATCTATGTTA CTAGATCGGG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGGGGTACCG AGCTCTAGAA TTCGAGGCGG TTTGCGTATT GGCTAGAGCA GCTTGCCAAC ATGGTGGAGC ACGACACTCT CGTCTACTCC AAGAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCTA TTGAGACTTT TCAACAAAGG GTAATATCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCAAAAGG ACAGTAGAAA AGGAAGGTGG CACCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC GTTCAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATAACATG GTGGAGCACG ACACTCTCGT CTACTCCAAG AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCTATTG AGACTTTTCA ACAAAGGGTA ATATCGGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTCAT CAAAAGGACA GTAGAAAAGG AAGGTGGCAC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCTATCGTT CAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACACGCTGAA ATCACCAGTC TCTCTCTACA AATCTATCTC TCTCGAGTCT ACCATGAGCC CAGAACGACG CCCGGCCGAC ATCCGCCGTG CCACCGAGGC GGACATGCCG GCGGTCTGCA CCATCGTCAA CCACTACATC GAGACAAGCA CGGTCAACTT CCGTACCGAG CCGCAGGAAC CGCAGGAGTG GACGGACGAC CTCGTCCGTC TGCGGGAGCG CTATCCCTGG CTCGTCGCCG AGGTGGACGG CGAGGTCGCC GGCATCGCCT ACGCGGGCCC CTGGAAGGCA CGCAACGCCT ACGACTGGAC GGCCGAGTCG ACCGTGTACG TCTCCCCCCG CCACCAGCGG ACGGGACTGG GCTCCACGCT CTACACCCAC CTGCTGAAGT CCCTGGAGGC ACAGGGCTTC AAGAGCGTGG TCGCTGTCAT CGGGCTGCCC AACGACCCGA GCGTGCGCAT GCACGAGGCG CTCGGATATG CCCCCCGCGG CATGCTGCGG GCGGCCGGCT TCAAGCACGG GAACTGGCAT GACGTGGGTT TCTGGCAGCT GGACTTCAGC CTGCCGGTAC CGCCCCGTCC GGTCCTGCCC GTCACCGAGA TTTGACTCGA GTTTCTCCAT AATAATGTGT GAGTAGTTCC CAGATAAGGG AATTAGGGTT CCTATAGGGT TTCGCTCATG TGTTGAGCAT ATAAGAAACC CTTAGTATGT ATTTGTATTT GTAAAATACT TCTATCAATA AAATTTCTAA TTCCTAAAAC CAAAATCCAG TACTAAAATC CAGATCCCCC GAATTAATTC GGCGTTAATT CAGTACATTA AAAACGTCCG CAATGTGTTA TTAAGTTGTC ACTAGTCAGG TTAACTCAAT TCGGCGTTAA TTCAGTACAT TAAAAACGTC CGCAATGTGT TATTAAGTTG TCTAAGCGTC AATTTGTTTA CACCACAATA TATCCTGCCA CCAGCCAGCC AACAGCTCCC CGACCGGCAG CTCGGCACAA AATCACCACT CGATACAGGC AGCCCATCAG TCCGGGACGG CGTCAGCGGG AGAGCCGTTG TAAGGCGGCA GACTTTGCTC ATGTTACCGA TGCTATTCGG AAGAACGGCA ACTAAGCTGC CGGGTTTGAA ACACGGATGA TCTCGCGGAG GGTAGCATGT TGATTGTAAC GATGACAGAG CGTTGCTGCC TGTGATCACT TAAGTAACTA ACTAACAGGA AGAGTTTGTA GAAACGCAAA AAGGCCATCC GTCAGGATGG CCTTCTGCTT AGTTTGATGC CTGGCAGTTT ATGGCGGGCG TCCTGCCCGC CACCCTCCGG GCCGTTGCTT CACAACGTTC AAATCCGCTC CCGGCGGATT TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTC CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCT TAGTAGTTAG ACGTCCCCGA GATCCATGCT AGACCATGAA TCCAGAAGCC CGAGAGGTTG CCGCCTTTCG GGCTTTTTCT TTTTCAAAAA AAAAAATTTA TAAAACGATC TGTTGCGGCC GGCCGCCGGG TTGTGGGCAA AGGCGCTCGA CGGTGGGCAA CCGCTTGCGG TTGTCCACGG GCGGAGCCGG TGCGCGTAGC GCATTGTCCA CAAGCCAAGG GCGACCAATA ATTGATATAT ATATTCATAA TTGAAAAGCT AATTGAACAT ACTACTTGCT GTAACTACTT GCCGGAGCGA GGGGTGTTTG CAAGCTGTTG ATCTGAAAGG GCTATTAGCG TTCTCACGTG CCTTTTTGAT TAGCGATTTC ACGTGACCTT ATTAGCGATT TCACGTACTC CGATTAGCGA TTTCACGTAC CCTGATTAGC GATTTCACGT GGATAGTTTT TGGAGCGGGC CGGAAAGCCC CGTGAATCAA GGCTTTGCGG GGCATTAGCG GTTTCACGTG GATAACTACC CTCTATCCAC AGGCTTCCGG GGATAAAAAA GCCCGCTCGA CGGCGGGCTG TTGGATGGGG ATCTAGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACCGGGTC TGACGCTCAG TGGAACGGGG CCCAATCTGA ATAATGTTAC AACCAATTAA CCAATTCTGA TTAGAAAAAC TCATCGAGCA TCAAATGAAA CTGCAATTTA TTCATATCAG GATTATCAAT ACCATATTTT TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA TAGGATGGCA AGATCCTGGT ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC TATTAATTTC CCCTCGTCAA AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC TGAATCCGGT GAGAATGGCA AAAGTTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA GCCATTACGC TCGTCATCAA AATCACTCGC ATCAACCAAA CCGTTATTCA TTCGTGATTG CGCCTGAGCG AGACGAAATA CGCGATCGCT GTTAAAAGGA CAATTACAAA CAGGAATCGA ATGCAACCGG CGCAGGGACA CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA TTCTTCTAAT ACCTGGAATG CTGTTTTTCC GGGGATCGCA GTGGTGAGTA ACCATGCATC ATCAGGAGTA CGGATAAAAT GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT TAGTCTGACC ATCTCATCTG TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA CAACTCTGGC GCATCGGGCT TCCCATACAA GCGATAGATT GTCGCACCTG ATTGCCCGAC ATTATCGCGA GCCCATTTAT ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG CCTCGACGTT TCCCGTTGAA TATGGCTCAT AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT TTGAGACACG GGCCAGAGCT GCAGTTTGAT CCCGAGGGGA ACCCTGTGGT TGACATGCAC ATACAAATGG ACGAACGGAT AAACCTTTTC ACGCCCTTTT AAATATCCGT TATTCTAATA AACGCTCTTT TCTCTTAG
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