pHSN6A01-AtU6-ALKBH10B-sgRNA1-35S-Cas9
100μg liquid plasmid(Instantly):$105
100μg liquid plasmid(Instantly):$105
| name | pHSN6A01-AtU6-ALKBH10B-sgRNA1-35S-Cas9 | another name | apHSN6A01-AtU6-ALKBH10B-sgRNA1-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan,Spe | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | AtU6-26 | Replicon | pSa |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGCTCTTTTC TCTTAGGTTT ACCCGCCAAT ATATCCTGTC AAACACTGAT AGTTTAAACT GAAGGCGGGA AACGACAATC TGATCCAAGC TCAAGCTAAG CTCACGTGAC GGAATTAAGC TTCGACTTGC CTTCCGCACA ATACATCATT TCTTCTTAGC TTTTTTTCTT CTTCTTCGTT CATACAGTTT TTTTTTGTTT ATCAGCTTAC ATTTTCTTGA ACCGTAGCTT TCGTTTTCTT CTTTTTAACT TTCCATTCGG AGTTTTTGTA TCTTGTTTCA TAGTTTGTCC CAGGATTAGA ATGATTAGGC ATCGAACCTT CAAGAATTTG ATTGAATAAA ACATCTTCAT TCTTAAGATA TGAAGATAAT CTTCAAAAGG CCCCTGGGAA TCTGAAAGAA GAGAAGCAGG CCCATTTATA TGGGAAAGAA CAATAGTATT TCTTATATAG GCCCATTTAA GTTGAAAACA ATCTTCAAAA GTCCCACATC GCTTAGATAA GAAAACGAAG CTGAGTTTAT ATACAGCTAG AGTCGAAGTA GTGATTGGCA CTCTCTTGTA GCTGACGGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTT TGCAAAATTT TCCAGATCGA TTTCTTCTTC CTCTGTTCTT CGGCGTTCAA TTTCTGGGGT TTTCTCTTCG TTTTCTGTAA CTGAAACCTA AAATTTGACC TAAAAAAAAT CTCAAATAAT ATGATTCAGT GGTTTTGTAC TTTTCAGTTA GTTGAGTTTT GCAGTTCCGA TGAGATAAAC CAATAAGCTT GCATGCCTGC AGGTCAACAT GGTGGAGCAC GACACACTTG TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATAACATGGT GGAGCACGAC ACACTTGTCT ACTCCAAAAA TATCAAAGAT ACAGTCTCAG AAGACCAAAG GGCAATTGAG ACTTTTCAAC AAAGGGTAAT ATCCGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTTATTG TGAAGATAGT GGAAAAGGAA GGTGGCTCCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG CCATCGTTGA AGATGCCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA AAAAGAAGAC GTTCCAACCA CGTCTTCAAA GCAAGTGGAT TGATGTGATA TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGGA CCTCGACCTC AACACAACAT ATACAAAACA AACGAATCTC AAGCAATCAA GCATTCTACT TCTATTGCAG CAATTTAAAT CATTTCTTTT AAAGCAAAAG CAATTTTCTG AAAATTTTCA CCATTTACGA ACGATACTCG AGGGGGATCC CCAATACTTG TATGGCCGCG GCCGCTCTAG ATGGATTACA AGGACCACGA CGGGGATTAC AAGGACCACG ACATTGATTA CAAGGATGAT GATGACAAGA TGGCTCCGAA GAAGAAGAGG AAGGTTGGCA TCCACGGGGT GCCAGCTGCT GACAAGAAGT ACTCGATCGG CCTCGCCATT GGGACTAACT CTGTTGGCTG GGCCGTGATC ACCGACGAGT ACAAGGTGCC CTCAAAGAAG TTCAAGGTCC TGGGCAACAC CGATCGGCAT TCCATCAAGA AGAATCTCAT TGGCGCTCTC CTGTTCGACA GCGGCGAGAC GGCTGAGGCT ACGCGGCTCA AGCGCACCGC CCGCAGGCGG TACACGCGCA GGAAGAATCG CATCTGCTAC CTGCAGGAGA TTTTCTCCAA CGAGATGGCG AAGGTTGACG ATTCTTTCTT CCACAGGCTG GAGGAGTCAT TCCTCGTGGA GGAGGATAAG AAGCACGAGC GGCATCCAAT CTTCGGCAAC ATTGTCGACG AGGTTGCCTA CCACGAGAAG TACCCTACGA TCTACCATCT GCGGAAGAAG CTCGTGGACT CCACAGATAA GGCGGACCTC CGCCTGATCT ACCTCGCTCT GGCCCACATG ATTAAGTTCA GGGGCCATTT CCTGATCGAG GGGGATCTCA ACCCGGACAA TAGCGATGTT GACAAGCTGT TCATCCAGCT CGTGCAGACG TACAACCAGC TCTTCGAGGA GAACCCCATT AATGCGTCAG GCGTCGACGC GAAGGCTATC CTGTCCGCTA GGCTCTCGAA GTCTCGGCGC CTCGAGAACC TGATCGCCCA GCTGCCGGGC GAGAAGAAGA ACGGCCTGTT CGGGAATCTC ATTGCGCTCA GCCTGGGGCT CACGCCCAAC TTCAAGTCGA ATTTCGATCT CGCTGAGGAC GCCAAGCTGC AGCTCTCCAA GGACACATAC GACGATGACC TGGATAACCT CCTGGCCCAG ATCGGCGATC AGTACGCGGA CCTGTTCCTC GCTGCCAAGA ATCTGTCGGA CGCCATCCTC CTGTCTGATA TTCTCAGGGT GAACACCGAG ATTACGAAGG CTCCGCTCTC AGCCTCCATG ATCAAGCGCT ACGACGAGCA CCATCAGGAT CTGACCCTCC TGAAGGCGCT GGTCAGGCAG CAGCTCCCCG AGAAGTACAA GGAGATCTTC TTCGATCAGT CGAAGAACGG CTACGCTGGG TACATTGACG GCGGGGCCTC TCAGGAGGAG TTCTACAAGT TCATCAAGCC GATTCTGGAG AAGATGGACG GCACGGAGGA GCTGCTGGTG AAGCTCAATC GCGAGGACCT CCTGAGGAAG CAGCGGACAT TCGATAACGG CAGCATCCCA CACCAGATTC ATCTCGGGGA GCTGCACGCT ATCCTGAGGA GGCAGGAGGA CTTCTACCCT TTCCTCAAGG ATAACCGCGA GAAGATCGAG AAGATTCTGA CTTTCAGGAT CCCGTACTAC GTCGGCCCAC TCGCTAGGGG CAACTCCCGC TTCGCTTGGA TGACCCGCAA GTCAGAGGAG ACGATCACGC CGTGGAACTT CGAGGAGGTG GTCGACAAGG GCGCTAGCGC TCAGTCGTTC ATCGAGAGGA TGACGAATTT CGACAAGAAC CTGCCAAATG AGAAGGTGCT CCCTAAGCAC TCGCTCCTGT ACGAGTACTT CACAGTCTAC AACGAGCTGA CTAAGGTGAA GTATGTGACC GAGGGCATGA GGAAGCCGGC TTTCCTGTCT GGGGAGCAGA AGAAGGCCAT CGTGGACCTC CTGTTCAAGA CCAACCGGAA GGTCACGGTT AAGCAGCTCA AGGAGGACTA CTTCAAGAAG ATTGAGTGCT TCGATTCGGT CGAGATCTCT GGCGTTGAGG ACCGCTTCAA CGCCTCCCTG GGGACCTACC ACGATCTCCT GAAGATCATT AAGGATAAGG ACTTCCTGGA CAACGAGGAG AATGAGGATA TCCTCGAGGA CATTGTGCTG ACACTCACTC TGTTCGAGGA CCGGGAGATG ATCGAGGAGC GCCTGAAGAC TTACGCCCAT CTCTTCGATG ACAAGGTCAT GAAGCAGCTC AAGAGGAGGA GGTACACCGG CTGGGGGAGG CTGAGCAGGA AGCTCATCAA CGGCATTCGG GACAAGCAGT CCGGGAAGAC GATCCTCGAC TTCCTGAAGA GCGATGGCTT CGCGAACCGC AATTTCATGC AGCTGATTCA CGATGACAGC CTCACATTCA AGGAGGATAT CCAGAAGGCT CAGGTGAGCG GCCAGGGGGA CTCGCTGCAC GAGCATATCG CGAACCTCGC TGGCTCGCCA GCTATCAAGA AGGGGATTCT GCAGACCGTG AAGGTTGTGG ACGAGCTGGT GAAGGTCATG GGCAGGCACA AGCCTGAGAA CATCGTCATT GAGATGGCCC GGGAGAATCA GACCACGCAG AAGGGCCAGA AGAACTCACG CGAGAGGATG AAGAGGATCG AGGAGGGCAT TAAGGAGCTG GGGTCCCAGA TCCTCAAGGA GCACCCGGTG GAGAACACGC AGCTGCAGAA TGAGAAGCTC TACCTGTACT ACCTCCAGAA TGGCCGCGAT ATGTATGTGG ACCAGGAGCT GGATATTAAC AGGCTCAGCG ATTACGACGT CGATGCCATC GTTCCACAGT CATTCCTGAA GGATGACTCC ATTGACAACA AGGTCCTCAC CAGGTCGGAC AAGAACCGGG GCAAGTCTGA TAATGTTCCT TCAGAGGAGG TCGTTAAGAA GATGAAGAAC TACTGGCGCC AGCTCCTGAA TGCCAAGCTG ATCACGCAGC GGAAGTTCGA TAACCTCACA AAGGCTGAGA GGGGCGGGCT CTCTGAGCTG GACAAGGCGG GCTTCATCAA GAGGCAGCTG GTCGAGACAC GGCAGATCAC TAAGCACGTT GCGCAGATTC TCGACTCACG GATGAACACT AAGTACGATG AGAATGACAA GCTGATCCGC GAGGTGAAGG TCATCACCCT GAAGTCAAAG CTCGTCTCCG ACTTCAGGAA GGATTTCCAG TTCTACAAGG TTCGGGAGAT CAACAATTAC CACCATGCCC ATGACGCGTA CCTGAACGCG GTGGTCGGCA CAGCTCTGAT CAAGAAGTAC CCAAAGCTCG AGAGCGAGTT CGTGTACGGG GACTACAAGG TTTACGATGT GAGGAAGATG ATCGCCAAGT CGGAGCAGGA GATTGGCAAG GCTACCGCCA AGTACTTCTT CTACTCTAAC ATTATGAATT TCTTCAAGAC AGAGATCACT CTGGCCAATG GCGAGATCCG GAAGCGCCCC CTCATCGAGA CGAACGGCGA GACGGGGGAG ATCGTGTGGG ACAAGGGCAG GGATTTCGCG ACCGTCAGGA AGGTTCTCTC CATGCCACAA GTGAATATCG TCAAGAAGAC AGAGGTCCAG ACTGGCGGGT TCTCTAAGGA GTCAATTCTG CCTAAGCGGA ACAGCGACAA GCTCATCGCC CGCAAGAAGG ACTGGGATCC GAAGAAGTAC GGCGGGTTCG ACAGCCCCAC TGTGGCCTAC TCGGTCCTGG TTGTGGCGAA GGTTGAGAAG GGCAAGTCCA AGAAGCTCAA GAGCGTGAAG GAGCTGCTGG GGATCACGAT TATGGAGCGC TCCAGCTTCG AGAAGAACCC GATCGATTTC CTGGAGGCGA AGGGCTACAA GGAGGTGAAG AAGGACCTGA TCATTAAGCT CCCCAAGTAC TCACTCTTCG AGCTGGAGAA CGGCAGGAAG CGGATGCTGG CTTCCGCTGG CGAGCTGCAG AAGGGGAACG AGCTGGCTCT GCCGTCCAAG TATGTGAACT TCCTCTACCT GGCCTCCCAC TACGAGAAGC TCAAGGGCAG CCCCGAGGAC AACGAGCAGA AGCAGCTGTT CGTCGAGCAG CACAAGCATT ACCTCGACGA GATCATTGAG CAGATTTCCG AGTTCTCCAA GCGCGTGATC CTGGCCGACG CGAATCTGGA TAAGGTCCTC TCCGCGTACA ACAAGCACCG CGACAAGCCA ATCAGGGAGC AGGCTGAGAA TATCATTCAT CTCTTCACCC TGACGAACCT CGGCGCCCCT GCTGCTTTCA AGTACTTCGA CACAACTATC GATCGCAAGA GGTACACAAG CACTAAGGAG GTCCTGGACG CGACCCTCAT CCACCAGTCG ATTACCGGCC TCTACGAGAC GCGCATCGAC CTGTCTCAGC TCGGGGGCGA CAAGCGGCCA GCGGCGACGA AGAAGGCGGG GCAGGCGAAG AAGAAGAAGT CCGGGTCATC AGGCAGCCCT AAGAAGAAGA GGAAGGTCGG CAGCAGCGGC AGCCCCAAGA AGAAGAGGAA GGTCGGGTCG GACGCCCTCG ACGATTTCGA CCTCGATATG CTGGGCTCCG ACGCCCTGGA CGACTTCGAC CTCGATATGC TCGGGAGCGA CGCGCTCGAC GACTTTGACC TCGATATGCT CGGGTCGGAC GCTCTGGACG ATTTTGACCT CGATATGCTG GGGTCGCCTA AGAAGAAGCG GAAGGTTGGC TCATACCCAT ACGATGTGCC CGACTACGCC TCCTGAGCTC AGGCCTCCAT ACAAGTATTG GGGATCCACT ACGAATTTCC CCGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC GCGGTGTCAT CTATGTTACT AGATCGGGAA TTCGAGGCGG TTTGCGTATT GGCTAGAGCA GCTTGCCAAC ATGGTGGAGC ACGACACTCT CGTCTACTCC AAGAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCTA TTGAGACTTT TCAACAAAGG GTAATATCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCAAAAGG ACAGTAGAAA AGGAAGGTGG CACCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC GTTCAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TGAAATCACC AGTCTCTCTC TACAAATCTA TCTCTCTCGA GCTTTCGCAG ATCCGGGGGG CAATGAGATA TGAAAAAGCC TGAACTCACC GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAGTTTAG CGAGAGCCTG ACCTATTGCA TCTCCCGCCG TTCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG TTCTACAACC GGTCGCGGAG GCTATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCAC GACTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGA AATAGAGTAG ATGCCGACCG GGATCTGTCG ATCGACAAGC TCGAGTTTCT CCATAATAAT GTGTGAGTAG TTCCCAGATA AGGGAATTAG GGTTCCTATA GGGTTTCGCT CATGTGTTGA GCATATAAGA AACCCTTAGT ATGTATTTGT ATTTGTAAAA TACTTCTATC AATAAAATTT CTAATTCCTA AAACCAAAAT CCAGTACTAA AATCCAGATC CCCCGAATTA ATTCGGCGTT AATTCAGTAC ATTAAAAACG TCCGCAATGT GTTATTAAGT TGTCACTAGT CAGGTTAACT CAATTCGGCG TTAATTCAGT ACATTAAAAA CGTCCGCAAT GTGTTATTAA GTTGTCTAAG CGTCAATTTG TTTACACCAC AATATATCCT GCCACCAGCC AGCCAACAGC TCCCCGACCG GCAGCTCGGC ACAAAATCAC CACTCGATAC AGGCAGCCCA TCAGTCCGGG ACGGCGTCAG CGGGAGAGCC GTTGTAAGGC GGCAGACTTT GCTCATGTTA CCGATGCTAT TCGGAAGAAC GGCAACTAAG CTGCCGGGTT TGAAACACGG ATGATCTCGC GGAGGGTAGC ATGTTGATTG TAACGATGAC AGAGCGTTGC TGCCTGTGAT CACTTAAGTA ACTAACTAAC AGGAAGAGTT TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA AAGGCCCAGT CTTCCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT CGCTTAGTAG TTAGACGTCC CCGAGATCCA TGCTAGACCA TGAATCCAGA AGCCCGAGAG GTTGCCGCCT TTCGGGCTTT TTCTTTTTCA AAAAAAAAAA TTTATAAAAC GATCTGTTGC GGCCGGCCGC CGGGTTGTGG GCAAAGGCGC TCGACGGTGG GCAACCGCTT GCGGTTGTCC ACGGGCGGAG CCGGTGCGCG TAGCGCATTG TCCACAAGCC AAGGGCGACC AATAATTGAT ATATATATTC ATAATTGAAA AGCTAATTGA ACATACTACT TGCTGTAACT ACTTGCCGGA GCGAGGGGTG TTTGCAAGCT GTTGATCTGA AAGGGCTATT AGCGTTCTCA CGTGCCTTTT TGATTAGCGA TTTCACGTGA CCTTATTAGC GATTTCACGT ACTCCGATTA GCGATTTCAC GTACCCTGAT TAGCGATTTC ACGTGGATAG TTTTTGGAGC GGGCCGGAAA GCCCCGTGAA TCAAGGCTTT GCGGGGCATT AGCGGTTTCA CGTGGATAAC TACCCTCTAT CCACAGGCTT CCGGGGATAA AAAAGCCCGC TCGACGGCGG GCTGTTGGAT GGGGATCTAG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GGGGCCCAAT CTGAATAATG TTACAACCAA TTAACCAATT CTGATTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC GACTCGTCCA ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA GGTTATCAAG TGAGAAATCA CCATGAGTGA CGACTGAATC CGGTGAGAAT GGCAAAAGTT TATGCATTTC TTTCCAGACT TGTTCAACAG GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA CAAACAGGAA TCGAATGCAA CCGGCGCAGG GACACTGCCA GCGCATCAAC AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT TTCCGGGGAT CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG AGGCATAAAT TCCGTCAGCC AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG CCATGTTTCA GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAAGCGATA GATTGTCGCA CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC ATCCATGTTG GAATTTAATC GCGGCCTCGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACGGGCCAG AGCTGCAGTT TGATCCCGAG GGGAACCCTG TGGTTGACAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGTTATTCT AATAAA