NM_001297650.2;CD163B; M160; SCARI2; WC1
100μg liquid plasmid(Instantly):$149
| name | pLVX-EnCMV-3×FLAG-CD163L1(human)(1synonymous mutations)-PGK-Puro | another name | apLVX-EnCMV-3×FLAG-CD163L1(human)(1synonymous mutations)-PGK-Puro |
| Insert Size (bp) | 4392bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC TACTAGAGGA TCGCTAGCGC TACCGGACTC AGATCTCGAG CCACCATGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGATGATG CTGCCTCAAA ACTCGTGGCA TATTGATTTT GGAAGATGCT GCTGTCATCA GAACCTTTTC TCTGCTGTGG TAACTTGCAT CCTGCTCCTG AATTCCTGCT TTCTCATCAG CAGTTTTAAT GGAACAGATT TGGAGTTGAG GCTGGTCAAT GGAGACGGTC CCTGCTCTGG GACAGTGGAG GTGAAATTCC AGGGACAGTG GGGGACTGTG TGTGATGATG GGTGGAACAC TACTGCCTCA ACTGTCGTGT GCAAACAGCT TGGATGTCCA TTTTCTTTCG CCATGTTTCG TTTTGGACAA GCCGTGACTA GACATGGAAA AATTTGGCTT GATGATGTTT CCTGTTATGG AAATGAGTCA GCTCTCTGGG AATGTCAACA CCGGGAATGG GGAAGCCATA ACTGTTATCA TGGAGAAGAT GTTGGTGTGA ACTGTTATGG TAAGAGTCTA ACTAGAGTAG GAAGGCAAGG TGAAGCCAAT CTGGGTTTGA GGCTAGTGGA TGGAAACAAC TCCTGTTCAG GGAGAGTGGA GGTGAAATTC CAAGAAAGGT GGGGAACTAT ATGTGATGAT GGGTGGAACT TGAATACTGC TGCCGTGGTG TGCAGGCAAC TAGGATGTCC ATCTTCTTTT ATTTCTTCTG GAGTTGTTAA TAGCCCTGCT GTATTGCGCC CCATTTGGCT GGATGACATT TTATGCCAGG GGAATGAGTT GGCACTCTGG AATTGCAGAC ATCGTGGATG GGGAAATCAT GACTGCAGTC ACAATGAGGA TGTCACATTA ACTTGTTATG ATAGTAGTGA TCTTGAACTA AGGCTTGTAG GTGGAACTAA CCGCTGTATG GGGAGAGTAG AGCTGAAAAT CCAAGGAAGG TGGGGGACCG TATGCCACCA TAAGTGGAAC AATGCTGCAG CTGATGTCGT ATGCAAGCAG TTGGGATGTG GAACCGCACT TCACTTCGCT GGCTTGCCTC ATTTGCAGTC AGGGTCTGAT GTTGTATGGC TTGATGGTGT CTCCTGCTCC GGTAATGAAT CTTTTCTTTG GGACTGCAGA CATTCCGGAA CCGTCAATTT TGACTGTCTT CATCAAAACG ATGTGTCTGT GATCTGCTCA GATGGAGCAG ATTTGGAACT GCGACTAGCA GATGGAAGTA ACAATTGTTC AGGGAGAGTA GAGGTGAGAA TTCATGAACA GTGGTGGACA ATATGTGACC AGAACTGGAA GAATGAACAA GCCCTTGTGG TTTGTAAGCA GCTAGGATGT CCGTTCAGCG TCTTTGGCAG TCGTCGTGCT AAACCTAGTA ATGAAGCTAG AGACATTTGG ATAAACAGCA TATCTTGCAC TGGGAATGAG TCAGCTCTCT GGGACTGCAC ATATGATGGA AAAGCAAAGC GAACATGCTT CCGAAGATCA GATGCTGGAG TAATTTGTTC TGATAAGGCA GATCTGGACC TAAGGCTTGT CGGGGCTCAT AGCCCCTGTT ATGGGAGATT GGAGGTGAAA TACCAAGGAG AGTGGGGGAC TGTGTGTCAT GACAGATGGA GCACAAGGAA TGCAGCTGTT GTTTGTAAAC AATTGGGATG TGGAAAGCCT TTGCATGTGT TTGGTATGAC CTATTTTAAA GAAGCATCAG GACCTATTTG GCTGGATGAC GTTTCTTGCA TTGGAAATGA GTCAAATATC TGGGACTGTG AACACAGTGG ATGGGGAAAG CATAATTGTG TACACAGAGA GGATGTGATT GTAACCTGCT CAGGTGATGC AACATGGGGC CTGAGGCTGG TGGGCGGCAG CAACCGCTGC TCGGGAAGAC TGGAGGTGTA CTTTCAAGGA CGGTGGGGCA CAGTGTGTGA TGACGGCTGG AACAGTAAAG CTGCAGCTGT GGTGTGTAGC CAGCTGGACT GCCCATCTTC TATCATTGGC ATGGGTCTGG GAAACGCTTC TACAGGATAT GGAAAAATTT GGCTCGATGA TGTTTCCTGT GATGGAGATG AGTCAGATCT CTGGTCATGC AGGAACAGTG GGTGGGGAAA TAATGACTGC AGTCACAGTG AAGATGTTGG AGTGATCTGT TCTGATGCAT CGGATATGGA GCTGAGGCTT GTGGGTGGAA GCAGCAGGTG TGCTGGAAAA GTTGAGGTGA ATGTCCAGGG TGCCGTGGGA ATTCTGTGTG CTAATGGCTG GGGAATGAAC ATTGCTGAAG TTGTTTGCAG GCAACTTGAA TGTGGGTCTG CAATCAGGGT CTCCAGAGAG CCTCATTTCA CAGAAAGAAC ATTACACATC TTAATGTCGA ATTCTGGCTG CACTGGAGGG GAAGCCTCTC TCTGGGATTG TATACGATGG GAGTGGAAAC AGACTGCGTG TCATTTAAAT ATGGAAGCAA GTTTGATCTG CTCAGCCCAC AGGCAGCCCA GGCTGGTTGG AGCTGATATG CCCTGCTCTG GACGTGTTGA AGTGAAACAT GCAGACACAT GGCGCTCTGT CTGTGATTCT GATTTCTCTC TTCATGCTGC CAATGTGCTG TGCAGAGAAT TAAACTGTGG AGATGCCATA TCTCTTTCTG TGGGAGATCA CTTTGGAAAA GGGAATGGTC TAACTTGGGC CGAAAAGTTC CAGTGTGAAG GGAGTGAAAC TCACCTTGCA TTATGCCCCA TTGTTCAACA TCCGGAAGAC ACTTGTATCC ACAGCAGAGA AGTTGGAGTT GTCTGTTCCC GATATACAGA TGTCCGACTT GTGAATGGCA AATCCCAGTG TGACGGGCAA GTGGAGATCA ACGTGCTTGG ACACTGGGGC TCACTGTGTG ACACCCACTG GGACCCAGAA GATGCCCGTG TTCTATGCAG ACAGCTCAGC TGTGGGACTG CTCTCTCAAC CACAGGAGGA AAATATATTG GAGAAAGAAG TGTTCGTGTG TGGGGACACA GGTTTCATTG CTTAGGGAAT GAGTCACTTC TGGATAACTG TCAAATGACA GTTCTTGGAG CACCTCCCTG TATCCATGGA AATACTGTCT CTGTGATCTG CACAGGAAGC CTGACCCAGC CACTGTTTCC ATGCCTCGCA AATGTATCTG ACCCATATTT GTCTGCAGTT CCAGAGGGCA GTGCTTTGAT CTGCTTAGAG GACAAACGGC TCCGCCTAGT GGATGGGGAC AGCCGCTGTG CCGGGAGAGT AGAGATCTAT CACGACGGCT TCTGGGGCAC CATCTGTGAT GACGGCTGGG ACCTGAGCGA TGCCCACGTG GTGTGTCAAA AGCTGGGCTG TGGAGTGGCC TTCAATGCCA CGGTCTCTGC TCACTTTGGG GAGGGGTCAG GGCCCATCTG GCTGGATGAC CTGAACTGCA CAGGAATGGA GTCCCACTTG TGGCAGTGCC CTTCCCGCGG CTGGGGGCAG CACGACTGCA GGCACAAGGA GGACGCAGGG GTCATCTGCT CAGAATTCAC AGCCTTGAGG CTCTACAGTG AAACTGAAAC AGAGAGCTGT GCTGGGAGAT TGGAAGTCTT CTATAACGGG ACCTGGGGCA GCGTCGGCAG GAGGAACATC ACCACAGCCA TAGCAGGCAT TGTGTGCAGG CAGCTGGGCT GTGGGGAGAA TGGAGTTGTC AGCCTCGCCC CTTTATCTAA GACAGGCTCT GGTTTCATGT GGGTGGATGA CATTCAGTGT CCTAAAACGC ATATCTCCAT ATGGCAGTGC CTGTCTGCCC CATGGGAGCG AAGAATCTCC AGCCCAGCAG AAGAGACCTG GATCACATGT GAAGATAGAA TAAGAGTGCG TGGAGGAGAC ACCGAGTGCT CTGGGAGAGT GGAGATCTGG CACGCAGGCT CCTGGGGCAC AGTGTGTGAT GACTCCTGGG ACCTGGCCGA GGCGGAAGTG GTGTGTCAGC AGCTGGGCTG TGGCTCTGCT CTGGCTGCCC TGAGGGACGC TTCGTTTGGC CAGGGAACTG GAACCATCTG GTTGGATGAC ATGCGGTGCA AAGGAAATGA GTCATTTCTA TGGGACTGTC ACGCCAAACC CTGGGGACAG AGTGACTGTG GACACAAGGA AGATGCTGGC GTGAGGTGCT CTGGACAGTC GCTGAAATCA CTGAATGCCT CCTCAGGTCA TTTAGCACTT ATTTTATCCA GTATCTTTGG GCTCCTTCTC CTGGTTCTGT TTATTCTATT TCTCACGTGG TGCCGAGTTC AGAAACAAAA ACATCTGCCC CTCAGAGTTT CAACCAGAAG GAGGGGTTCT CTCGAGGAGA ATTTATTCCA TGAGATGGAG ACCTGCCTCA AGAGAGAGGA CCCACATGGG ACAAGAACCT CAGATGACAC CCCCAACCAT GGTTGTGAAG ATGCTAGCGA CACATCGCTG TTGGGAGTTC TTCCTGCCTC TGAAGCCACA AAATGAGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA TCCCGCGACT CTAGATAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC TTTCGACCTG CAGCCCAAGC TTACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACCGCG TCTGGAACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAATTAATTC TGCAGTCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGAGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTAGCAGA ACTACACACC AGGGCCAGGG GTCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA GCCAGATAAG GTAGAAGAGG CCAATAAAGG AGAGAACACC AGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTGTTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACGTGGCCC GAGAGCTGCA TCCGGAGTAC TTCAAGAACT GCTGATATCG AGCTTGCTAC AAGGGACTTT CCGCTGGGGA CTTTCCAGGG AGGCGTGGCC TGGGCGGGAC TGGGGAGTGG CGAGCCCTCA GATCCTGCAT ATAAGCAGCT GCTTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGCC TTGACATTGC TAGCGTTTTA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCAACTGG ATAACTCAAG CTAACCAAAA TCATCCCAAA CTTCCCACCC CATACCCTAT TACCACTGCC AATTACCTGT GGTTTCATTT ACTCTAAACC TGTGATTCCT CTGAATTATT TTCATTTTAA AGAAATTGTA TTTGTTAAAT ATGTACTACA AACTTAGTAG TTTTTAAAGA AATTGTATTT GTTAAATATG TACTACAAAC TTAGTAGT
