PYCm-PK9-T82A-T265A-T269A
$84.00
In stock
SKU
PL55934
100μg liquid plasmid(Instantly):$105
| name | PYCm-PK9-T82A-T265A-T269A | another name | aPYCm-PK9-T82A-T265A-T269A |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCACTATCCA ACTACACTCA TAATGCTTAA AGAAAATTGG CATTATTTTA CCAATTTTTT TAAAACAAAA AAAGAATAAA ATATTTTCTT ATTATTTAGC TAAAATTGTA CTAAATTTTA TACCAAAAAA AATATATATA AATATATGTA ATATATAATA TATATATATA TATATGTATA TATGTATGTA TGTATATATG TATGTATGTA TATATGTATG TATGTATATA TGTATGTATG TATGTATAGT TTATATTTAT TTGGCAAGTT TTTCTGTAAA AATGTTTAAT TAAAAAAAAA AATATATAAC ATGCCAATAT GCACTATTCA TTTTATGGAG GCAATTTTAT GAGTAACCAT TTTTACTTTT TTTTTACATA TGCGCATACT TCGAGTTATA CAATGTTATT GGAGACGCGT AGAGGCCTCG TCTCAGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTTGTTC CTTTTGTTGT AGACATATAA TTGATCATTT TATTTTTTTA TTTATTATTT TCCAAATTCT TTAAATTTTT ATTACCCTTA AAAAATTTCC CATTTTTCCA TATAACCAAA CTTAGCTAGT TATGGCTAGT TTTGTATGAC CAATATTTTT TAATATTCCT AAGAATTTTT TTTTTTAATT TGTTTATAAA ATTATGGAAA ATCCAAGAAA TCACAATTAT CAATTTTTTA GTTAATTTCA CTTTTTATGT TTTCAATAAT TCCATCGAAA AGACACGAAA TGATATACTC CCTTGCACTT CACAAAAGTG CGAAATAAAA AATGTATAGT AAATAATATA ATATTAAATA CGTAGTATTT TTAGGCTTTC CTATATTAAA TTAATTATTC TCGACATATT TATTAATATA TAGTAAGAAA TGTAAATTTT CCAACAAGCA TATAACAATT ACTGTTCATT CAATATTGCC ATCTAAAATG GAATGGAATA TATTTAAAGT GGCTTCATAG AAAGGGGATA TTATTAAAGG TATTATTTTT AACTTTTTTT TTTTTATTTA ATTTTTAAAA TTTATATTTT AGTGCACTAT ATCTATTCGT GAAACATCGG AGAAAATATA TATATTCATA TATTTAGATG CACGTGGATG CATATAAACG TATTTACACA CACCCTTTCG TGTGAACTCT GATTATTGAT TCTATGAATT GAATCATATC AAGATGTATC TAGGGAGTGT CAAAAATGTG GAGGATTTGT TCCTGTCGAA GGGAGAATAC AAACAAGTAT TCCTTTGTTT AAAAAAAAAT GAAAATAGTG AGGCAATACA AATAGATATA AGCTGTGATA GCAACATTTA TAGTATCTTA CAATTATGCA GAGAAAAATA TGGAATCCAT GGTGATTATT TAACAGATAG TTGTGGAAAT ATTGTAGAAA ATATTAAAGA GATAATAGAT GGTGATGCTT TATATTTAAA AGATAATAAA GATAAATTTC ATTTTTTAAA TCAAATAAAA TCAAATAATT TAATAGTAAA TGATTATATA GTAGAAAAAA GAATAGGGTC AGGTGGATTT GGTATAGTAT TTCAAGGTAT ACATATACAA ACTAAACAAA AAGTTGCACT AAAATTTATA CCTAAAAGTA ATTTTTTAGA TGTTACAGAT GTACATAGAG TCTTTATAGA AATACAAACA TTAAGAGGTT TGATACATAA AAATATAATT AAAATGTATG ATGTTAATCA TTTTCAAAAT TATGTATGTC TAATTATGGA ATATGCTGTT AATAGTGATT TAAAAAAATA TATACAAAAA AATAATGGAT ATTTATCTGA AAAAGAAACA TATTTTTTAT TTTTGCAAAT TGTTAAAGGA GTATATTATT GTCATTCTAA GCATATTGTA CATAGAGATT TAAAATTAGA AAATATTTTA CTTGATGAAA ATATGACATG TAAAATTGCA GATTTTGGAT TATCTGACTT TGTTAATGTT GATCAAAATA TTAAAGCTGA AGCTGGAGCA AAATTATGAA TTCCTTAAGT TTACAATTTA ATATTCATAC TTTAAGTATT TTTTGTAGTA TCCTAGATAT TGTGCTTTAA ATGCTCACCC CTCAAAGCAC CAGTAATATT TTCATCCACT GAAATACCAT TAAATTTTCA AAAAAATACT ATGCATATAA TGTTATACAT ATAAACATAA AACGCCATGT AAATCAAAAA ATATATAAAA ATATGTATAA AAATAAATAT GCACTAAATA TAAGCTAATT ATGCATAAAA ATTAAAGTGC CCTTTATTAA CTAGTCGTAA TTATTTATAT TTCTATGTTA TAAAAAAATC CTCATATAAT AATATAATTA ATATATGTAA TGTTTTTTTT ATTTTATAAT TTTAATATAA AATAATATGT AAATTAATTC AAAAAATAAA TATAATTGTT GTGAAACAAA AAACGTAATT TTTTCATTTG CCTTCAAAAT TTAAATTTAT TTTAATATTT CCTAAAATAT ATATACTTTG TGTATAAATA TATAAAAATA TATATTTGCT TATAAATAAA TAAAAAATTT TATAAAACAT AGGGGGATCT ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC GGGGACCTGC CCTGGCCACC GCTCAGGAAC GAATTTAGAT ATTTCCAGAG AATGACCACA ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT GTTTATAAGG AAGCCATGAA TCACCCAGGC CATCTTAAAC TATTTGTGAC AAGGATCATG CAAGACTTTG AAAGTGACAC GTTTTTTCCA GAAATTGATT TGGAGAAATA TAAACTTCTG CCAGAATACC CAGGTGTTCT CTCTGATGTC CAGGAGGAGA AAGGCATTAA GTACAAATTT GAAGTATATG AGAAGAATGA TGCAAGCGGA GGAGGTGGAT CTGGTGGAGG TGGAAGTGCA AGCGTGACAG GGGGAATGGC TAGCAAGTGG GATCAGAAGG GTATGGACAT TGCCTATGAG GAGGCGGCCT TAGGTTACAA AGAGGGTGGT GTTCCTATTG GCGGATGTCT TATCAATAAC AAAGACGGAA GTGTTCTCGG TCGTGGTCAC AACATGAGAT TTCAAAAGGG ATCCGCCACA CTACATGGTG AGATCTCCAC TTTGGAAAAC TGTGGGAGAT TAGAGGGCAA AGTGTACAAA GATACCACTT TGTATACGAC GCTGTCTCCA TGCGACATGT GTACAGGTGC CATCATCATG TATGGTATTC CACGCTGTGT TGTCGGTGAG AACGTTAATT TCAAAAGTAA GGGCGAGAAA TATTTACAAA CTAGAGGTCA CGAGGTTGTT GTTGTTGACG ATGAGAGGTG TAAAAAGATC ATGAAACAAT TTATCGATGA AAGACCTCAG GATTGGTTTG AAGATATTGG TGAGGCTTCG GAACCATTTA AGAACGTCTA CTTGCTACCT CAAACAAACC AATTGCTGGG TTTGTACACC ATCATCAGAA ATAAGAATAC AACTAGACCT GATTTCATTT TCTACTCCGA TAGAATCATC AGATTGTTGG TTGAAGAAGG TTTGAACCAT CTACCTGTGC AAAAGCAAAT TGTGGAAACT GACACCAACG AAAACTTCGA AGGTGTCTCA TTCATGGGTA AAATCTGTGG TGTTTCCATT GTCAGAGCTG GTGAATCGAT GGAGCAAGGA TTAAGAGACT GTTGTAGGTC TGTGCGTATC GGTAAAATTT TAATTCAAAG GGACGAGGAG ACTGCTTTAC CAAAGTTATT CTACGAAAAA TTACCAGAGG ATATATCTGA AAGGTATGTC TTCCTATTAG ACCCAATGCT GGCCACCGGT GGTAGTGCTA TCATGGCTAC AGAAGTCTTG ATTAAGAGAG GTGTTAAGCC AGAGAGAATT TACTTCTTAA ACCTAATCTG TAGTAAGGAA GGGATTGAAA AATACCATGC CGCCTTCCCA GAGGTCAGAA TTGTTACTGG TGCCCTCGAC AGAGGTCTAG ATGAAAACAA GTATCTAGTT CCAGGGTTGG GTGACTTTGG TGACAGATAC TACTGTGTTG GTTCGGGAGA GGGCAGAGGA TCCCTGCTAA CATGCGGTGA TGTCGAGGAG AATCCTGGCC CAGAATCGAT GGACTATAAG GACCACGACG GAGACTACAA GGATCATGAT ATTGATTACA AAGACGATGA CGATAAGATG GCCCCAAAGA AGAAGCGGAA GGTCGGTATC CACGGAGTCC CAGCAGCCGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GTCTGCCAGA CTGAGCAAGA GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTCG GAAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT CAAGAGCAAC TTCGACCTGG CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA CGACGACCTG GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC CGCCAAGAAC CTGTCCGACG CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT CACCAAGGCC CCCCTGAGCG CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT GACCCTGCTG AAAGCTCTCG TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT CGACCAGAGC AAGAACGGCT ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT CTACAAGTTC ATCAAGCCCA TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA GCTGAACAGA GAGGACCTGC TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA CCAGATCCAC CTGGGAGAGC TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT CCTGAAGGAC AACCGGGAAA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT GGGCCCTCTG GCCAGGGGAA ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC CATCACCCCC TGGAACTTCG AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT CGAGCGGATG ACCAACTTCG ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG CCTGCTGTAC GAGTACTTCA CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA GGGAATGAGA AAGCCCGCCT TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT GTTCAAGACC AACCGGAAAG TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT CGAGTGCTTC GACTCCGTGG AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG CACATACCAC GATCTGCTGA AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA CGAGGACATT CTGGAAGATA TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT CGAGGAACGG CTGAAAACCT ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA GCGGCGGAGA TACACCGGCT GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA CAAGCAGTCC GGCAAGACAA TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA CTTCATGCAG CTGATCCACG ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA GGTGTCCGGC CAGGGCGATA GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC CATTAAGAAG GGCATCCTGC AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG CCGGCACAAG CCCGAGAACA TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA GGGACAGAAG AACAGCCGCG AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG CAGCCAGATC CTGAAAGAAC ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA CCTGTACTAC CTGCAGAATG GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG GCTGTCCGAC TACGATGTGG ACCATATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT CGACAACAAG GTGCTGACCA GAAGCGACAA GAACCGGGGC AAGAGCGACA ACGTGCCCTC CGAAGAGGTC GTGAAGAAGA TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT TACCCAGAGA AAGTTCGACA ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA TAAGGCCGGC TTCATCAAGA GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC ACAGATCCTG GACTCCCGGA TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA AGTGAAAGTG ATCACCCTGA AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT TTACAAAGTG CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT CGTGGGAACC GCCCTGATCA AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA CTACAAGGTG TACGACGTGC GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC TACCGCCAAG TACTTCTTCT ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT GGCCAACGGC GAGATCCGGA AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT CGTGTGGGAT AAGGGCCGGG ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT GAATATCGTG AAAAAGACCG AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC CAAGAGGAAC AGCGATAAGC TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG CGGCTTCGAC AGCCCCACCG TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG CAAGTCCAAG AAACTGAAGA GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG CAGCTTCGAG AAGAATCCCA TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA GGACCTGATC ATCAAGCTGC CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG AATGCTGGCC TCTGCCGGCG AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA TGTGAACTTC CTGTACCTGG CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA TGAGCAGAAA CAGCTGTTTG TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA GATCAGCGAG TTCTCCAAGA GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC CGCCTACAAC AAGCACCGGG ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT GTTTACCCTG ACCAATCTGG GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA CCGGAAGAGG TACACCAGCA CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT CACCGGCCTG TACGAGACAC GGATCGACCT GTCTCAGCTG GGAGGCGACA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC AGGCAAAAAA GAAAAAGTAA GAATTACCGG TGATCCCGTT TTTCTTACTT ATATATTTAT ACCAATTGAT TGTATTTATA ACTGTAAAAA TGTGTATGTT GTGTGCATAT TTTTTTTTGT GCATGCACAT GCATGTAAAT AGCTAAAATT ATGAACATTT TATTTTTTGT TCAGAAAAAA AAAACTTTAC ACACATAAAA TGGCTAGTAT GAATAGCCAT ATTTTATATA AATTAAATCC TATGAATTTA TGACCATATT AAAAATTTAG ATATTTATGG AACATAATAT GTTTGAAACA ATAAGACAAA ATTATTATTA TTATTATTAT TTTTACTGTT ATAATTATGT TGTCTCTTCA ATGATTCATA AATAGTTGGA CTTGATTTTT AAAATGTTTA TAATATGATT AGCATAGTTA AATAAAAAAA GTTGAAAAAT TAAAAAAAAA CATATAAACA CAAATGATGT TTTTTCCTTC AATTTCGG
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