pCWLIC-2×Tac-CYP7A1(human)(25-504aa)-6×His
$84.00
In stock
SKU
PL55863
100μg liquid plasmid(Instantly):$105
| name | pCWLIC-2×Tac-CYP7A1(human)(25-504aa)-6×His | another name | apCWLIC-2×Tac-CYP7A1(human)(25-504aa)-6×His |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Tac | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGAAC GCCATCAAAA ATAATTCGCG TCTGGCCTTC CTGTAGCCAG CTTTCATCAA CATTAAATGT GAGCGAGTAA CAACCCGTCG GATTCTCCGT GGGAACAAAC GGCGGATTGA CCGTAATGGG ATAGGTTACG TTGGTGTAGA TGGGCGCATC GTAACCGTGC ATCTGCCAGT TTGAGGGGAC GACGACAGTA TCGGCCTCAG GAAGATCGCA CTCCAGCCAG CTTTCCGGCA CCGCTTCTGG TGCCGGAAAC CAGGCAAAGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATCCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG ATGTCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG GTGGCAACGC CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCTCACGAGG TCCTCGAGTT GACAATTAAT CATCGGCTCG TATAATGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGGA TCAGCTTACT CCCCATCCCC CTGGGATCCA TCGATGCTTT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AAAGGAGGTC ATATGATGGC CAAGAAGACC AGCAGCAGGA GAAGGCAAAC GGGTGAACCA CCTCTAGAGA ATGGATTAAT TCCATACCTG GGCTGTGCTC TGCAATTTGG TGCCAATCCT CTTGAGTTCC TCAGAGCAAA TCAAAGGAAA CATGGTCATG TTTTTACCTG CAAACTAATG GGAAAATATG TCCATTTCAT CACAAATCCC TTGTCATACC ATAAGGTGTT GTGCCACGGA AAATATTTTG ATTGGAAAAA ATTTCACTTT GCTACTTCTG CGAAGGCATT TGGGCACAGA AGCATTGACC CGATGGATGG AAATACCACT GAAAACATAA ACGACACTTT CATCAAAACC CTGCAGGGCC ATGCCTTGAA TTCCCTCACG GAAAGCATGA TGGAAAACCT CCAACGTATC ATGAGACCTC CAGTCTCCTC TAACTCAAAG ACCGCTGCCT GGGTGACAGA AGGGATGTAT TCTTTCTGCT ACCGAGTGAT GTTTGAAGCT GGGTATTTAA CTATCTTTGG CAGAGATCTT ACAAGGCGGG ACACACAGAA AGCACATATT CTAAACAATC TTGACAACTT CAAGCAATTC GACAAAGTCT TTCCAGCCCT GGTAGCAGGC CTCCCCATTC ACATGTTCAG GACTGCGCAC AATGCCCGGG AGAAACTGGC AGAGAGCTTG AGGCACGAGA ACCTCCAAAA GAGGGAAAGC ATCTCAGAAC TGATCAGCCT GCGCATGTTT CTCAATGACA CTTTGTCCAC CTTTGATGAT CTGGAGAAGG CCAAGACACA CCTCGTGGTC CTCTGGGCAT CGCAAGCAAA CACCATTCCA GCGACTTTCT GGAGTTTATT TCAAATGATT AGGAACCCAG AAGCAATGAA AGCAGCTACT GAAGAAGTGA AAAGAACATT AGAGAATGCT GGTCAAAAAG TCAGCTTGGA AGGCAATCCT ATTTGTTTGA GTCAAGCAGA ACTGAATGAC CTGCCAGTAT TAGATAGTAT AATCAAGGAA TCGCTGAGGC TTTCCAGTGC CTCCCTCAAC ATCCGGACAG CTAAGGAGGA TTTCACTTTG CACCTTGAGG ACGGTTCCTA CAACATCCGA AAAGATGACA TCATAGCTCT TTACCCACAG TTAATGCACT TAGATCCAGA AATCTACCCA GACCCTTTGA CTTTTAAATA TGATAGGTAT CTTGATGAAA ACGGGAAGAC AAAGACTACC TTCTATTGTA ATGGACTCAA GTTAAAGTAT TACTACATGC CCTTTGGATC GGGAGCTACA ATATGTCCTG GAAGATTGTT CGCTATCCAC GAAATCAAGC AATTTTTGAT TCTGATGCTT TCTTATTTTG AATTGGAGCT TATAGAGGGC CAAGCTAAAT GTCCACCTTT GGACCAGTCC CGGGCAGGCT TGGGCATTTT GCCGCCATTG AATGATATTG AATTTAAATA TAAATTCAAG CATTTGCACC ATCACCATCA CCATTAGGAA AGATCCATAA CTTCGTATAG CATACATTAT ACGAAGTTAT GCGGCCGCGA CGTCCACATA TACCTGCCGT TCACTATTAT TTAGTGAAAT GAGATATTAT GATATTTTCT GAATTGTGAT TAAAAAGGCA ACTTTATGCC CATGCAACAG AAACTATAAA AAATACAGAG AATGAAAAGA AACAGATAGA TTTTTTAGTT CTTTAGGCCC GTAGTCTGCA AATCCTTTTA TGATTTTCTA TCAAACAAAA GAGGAAAATA GACCAGTTGC AATCCAAACG AGAGTCTAAT AGAATGAGGT CGAAAAGTAA ATCGCGCGGG TTTGTTACTG ATAAAGCAGG CAAGACCTAA AATGTGTAAA GGGCAAAGTG TATACTTTGG CGTCACCCCT TACATATTTT AGGTCTTTTT TTATTGTGCG TAACTAACTT GCCATCTTCA AACAGGAGGG CTGGAAGAAG CAGACCGCTA ACACAGTACA TAAAAAAGGA GACATGAACG ATGAACATCA AAAAGTTTGC AAAACAAGCA ACAGTATTAA CCTTTACTAC CGCACTGCTG GCAGGAGGCG CAACTCAAGC GTTTGCGAAA GAAACGAACC AAAAGCCATA TAAGGAAACA TACGGCATTT CCCATATTAC ACGCCATGAT ATGCTGCAAA TCCCTGAACA GCAAAAAAAT GAAAAATATC AAGTTCCTGA GTTCGATTCG TCCACAATTA AAAATATCTC TTCTGCAAAA GGCCTGGACG TTTGGGACAG CTGGCCATTA CAAAACGCTG ACGGCACTGT CGCAAACTAT CACGGCTACC ACATCGTCTT TGCATTAGCC GGAGATCCTA AAAATGCGGA TGACACATCG ATTTACATGT TCTATCAAAA AGTCGGCGAA ACTTCTATTG ACAGCTGGAA AAACGCTGGC CGCGTCTTTA AAGACAGCGA CAAATTCGAT GCAAATGATT CTATCCTAAA AGACCAAACA CAAGAATGGT CAGGTTCAGC CACATTTACA TCTGACGGAA AAATCCGTTT ATTCTACACT GATTTCTCCG GTAAACATTA CGGCAAACAA ACACTGACAA CTGCACAAGT TAACGTATCA GCATCAGACA GCTCTTTGAA CATCAACGGT GTAGAGGATT ATAAATCAAT CTTTGACGGT GACGGAAAAA CGTATCAAAA TGTACAGCAG TTCATCGATG AAGGCAACTA CAGCTCAGGC GACAACCATA CGCTGAGAGA TCCTCACTAC GTAGAAGATA AAGGCCACAA ATACTTAGTA TTTGAAGCAA ACACTGGAAC TGAAGATGGC TACCAAGGCG AAGAATCTTT ATTTAACAAA GCATACTATG GCAAAAGCAC ATCATTCTTC CGTCAAGAAA GTCAAAAACT TCTGCAAAGC GATAAAAAAC GCACGGCTGA GTTAGCAAAC GGCGCTCTCG GTATGATTGA GCTAAACGAT GATTACACAC TGAAAAAAGT GATGAAACCG CTGATTGCAT CTAACACAGT AACAGATGAA ATTGAACGCG CGAACGTCTT TAAAATGAAC GGCAAATGGT ACCTGTTCAC TGACTCCCGC GGATCAAAAA TGACGATTGA CGGCATTACG TCTAACGATA TTTACATGCT TGGTTATGTT TCTAATTCTT TAACTGGCCC ATACAAGCCG CTGAACAAAA CTGGCCTTGT GTTAAAAATG GATCTTGATC CTAACGATGT AACCTTTACT TACTCACACT TCGCTGTACC TCAAGCGAAA GGAAACAATG TCGTGATTAC AAGCTATATG ACAAACAGAG GATTCTACGC AGACAAACAA TCAACGTTTG CGCCGAGCTT CCTGCTGAAC ATCAAAGGCA AGAAAACATC TGTTGTCAAA GACAGCATCC TTGAACAAGG ACAATTAACA GTTAACAAAT AAAAACGCAA AAGAAAATGC CGATATTGAC GTCAGGTGGC ACTTTTCGAG GAGATCATGC ACATGATGAC GAAGCTTATC GATGATAAGC TGTCAAACAT GAGCAGATCT GAGCCCGCCT AATGAGCGGG CTTTTAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GGTGCTCGTC AAAGCAACTC CGGAT
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