pHSN501-AtU6-BRI1-sgRNA1-sgRNA2-35S-Cas9
$84.00
In stock
SKU
PL55853
100μg liquid plasmid(Instantly):$105
| name | pHSN501-AtU6-BRI1-sgRNA1-sgRNA2-35S-Cas9 | another name | apHSN501-AtU6-BRI1-sgRNA1-sgRNA2-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan,Spe | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | AtU6-26 | Replicon | pSa |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TAAGCTCACG TGACGGAATT AAGCTTCGAC TTGCCTTCCG CACAATACAT CATTTCTTCT TAGCTTTTTT TCTTCTTCTT CGTTCATACA GTTTTTTTTT GTTTATCAGC TTACATTTTC TTGAACCGTA GCTTTCGTTT TCTTCTTTTT AACTTTCCAT TCGGAGTTTT TGTATCTTGT TTCATAGTTT GTCCCAGGAT TAGAATGATT AGGCATCGAA CCTTCAAGAA TTTGATTGAA TAAAACATCT TCATTCTTAA GATATGAAGA TAATCTTCAA AAGGCCCCTG GGAATCTGAA AGAAGAGAAG CAGGCCCATT TATATGGGAA AGAACAATAG TATTTCTTAT ATAGGCCCAT TTAAGTTGAA AACAATCTTC AAAAGTCCCA CATCGCTTAG ATAAGAAAAC GAAGCTGAGT TTATATACAG CTAGAGTCGA AGTAGTGATT GTTACAGGCT AAGAGCATGA AGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTTGCAAA ATTTTCCAGA TCGATTTCTT CTTCCTCTGT TCTTCGGCCA TCGCTACCTT AGGACCGTTA TAGTTAATTA CCCTGTTATC CCTATTAATT AAGAGCTCAG AAATCTCAAA ATTCCGGCAG AACAATTTTG AATCTCGATC CGTAGAAACG AGACGGTCAT TGTTTTAGTT CCACCACGAT TATATTTGAA ATTTACGTGA GTGTGAGTGA GACTTGCATA AGAAAATAAA ATCTTTAGTT GGGAAAAAAT TCAATAATAT AAATGGGCTT GAGAAGGAAG CGAGGGATAG GCCTTTTTCT AAAATAGGCC CATTTAAGCT ATTAACAATC TTCAAAAGTA CCACAGCGCT TAGGTAAAGA AAGCAGCTGA GTTTATATAT GGTTAGAGAC GAAGTAGTGA TTGCGCAAAA AGCTACCACC CATGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCT TTTTTTTGCA AAATTTTCCA GATCGATTTC TTCTTCCTCT GTTCTTCGGC GTTCAATTTC TGGGGTTTTC TCTTCGTTTT CTGTAACTGA AACCTAAAAT TTGACCTAAA AAAAATCTCA AATAATATGA TTCAGTGGTT TTGTACTTTT CAGTTAGTTG AGTTTTGCAG TTCCGATGAG ATAAACCAAT AAGCTTGCAT GCCTGCAGGT CAACATGGTG GAGCACGACA CACTTGTCTA CTCCAAAAAT ATCAAAGATA CAGTCTCAGA AGACCAAAGG GCAATTGAGA CTTTTCAACA AAGGGTAATA TCCGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTTATTGT GAAGATAGTG GAAAAGGAAG GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC CATCGTTGAA GATGCCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGATAA CATGGTGGAG CACGACACAC TTGTCTACTC CAAAAATATC AAAGATACAG TCTCAGAAGA CCAAAGGGCA ATTGAGACTT TTCAACAAAG GGTAATATCC GGAAACCTCC TCGGATTCCA TTGCCCAGCT ATCTGTCACT TTATTGTGAA GATAGTGGAA AAGGAAGGTG GCTCCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCCAT CGTTGAAGAT GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGCAA GTGGATTGAT GTGATATCTC CACTGACGTA AGGGATGACG CACAATCCCA CTATCCTTCG CAAGACCCTT CCTCTATATA AGGAAGTTCA TTTCATTTGG AGAGGACCTC GACCTCAACA CAACATATAC AAAACAAACG AATCTCAAGC AATCAAGCAT TCTACTTCTA TTGCAGCAAT TTAAATCATT TCTTTTAAAG CAAAAGCAAT TTTCTGAAAA TTTTCACCAT TTACGAACGA TACTCGAGGG GGATCCCCAA TACTTGTATG GCCGCGGCCG CTCTAGATGG ATTACAAGGA CCACGACGGG GATTACAAGG ACCACGACAT TGATTACAAG GATGATGATG ACAAGATGGC TCCGAAGAAG AAGAGGAAGG TTGGCATCCA CGGGGTGCCA GCTGCTGACA AGAAGTACTC GATCGGCCTC GCCATTGGGA CTAACTCTGT TGGCTGGGCC GTGATCACCG ACGAGTACAA GGTGCCCTCA AAGAAGTTCA AGGTCCTGGG CAACACCGAT CGGCATTCCA TCAAGAAGAA TCTCATTGGC GCTCTCCTGT TCGACAGCGG CGAGACGGCT GAGGCTACGC GGCTCAAGCG CACCGCCCGC AGGCGGTACA CGCGCAGGAA GAATCGCATC TGCTACCTGC AGGAGATTTT CTCCAACGAG ATGGCGAAGG TTGACGATTC TTTCTTCCAC AGGCTGGAGG AGTCATTCCT CGTGGAGGAG GATAAGAAGC ACGAGCGGCA TCCAATCTTC GGCAACATTG TCGACGAGGT TGCCTACCAC GAGAAGTACC CTACGATCTA CCATCTGCGG AAGAAGCTCG TGGACTCCAC AGATAAGGCG GACCTCCGCC TGATCTACCT CGCTCTGGCC CACATGATTA AGTTCAGGGG CCATTTCCTG ATCGAGGGGG ATCTCAACCC GGACAATAGC GATGTTGACA AGCTGTTCAT CCAGCTCGTG CAGACGTACA ACCAGCTCTT CGAGGAGAAC CCCATTAATG CGTCAGGCGT CGACGCGAAG GCTATCCTGT CCGCTAGGCT CTCGAAGTCT CGGCGCCTCG AGAACCTGAT CGCCCAGCTG CCGGGCGAGA AGAAGAACGG CCTGTTCGGG AATCTCATTG CGCTCAGCCT GGGGCTCACG CCCAACTTCA AGTCGAATTT CGATCTCGCT GAGGACGCCA AGCTGCAGCT CTCCAAGGAC ACATACGACG ATGACCTGGA TAACCTCCTG GCCCAGATCG GCGATCAGTA CGCGGACCTG TTCCTCGCTG CCAAGAATCT GTCGGACGCC ATCCTCCTGT CTGATATTCT CAGGGTGAAC ACCGAGATTA CGAAGGCTCC GCTCTCAGCC TCCATGATCA AGCGCTACGA CGAGCACCAT CAGGATCTGA CCCTCCTGAA GGCGCTGGTC AGGCAGCAGC TCCCCGAGAA GTACAAGGAG ATCTTCTTCG ATCAGTCGAA GAACGGCTAC GCTGGGTACA TTGACGGCGG GGCCTCTCAG GAGGAGTTCT ACAAGTTCAT CAAGCCGATT CTGGAGAAGA TGGACGGCAC GGAGGAGCTG CTGGTGAAGC TCAATCGCGA GGACCTCCTG AGGAAGCAGC GGACATTCGA TAACGGCAGC ATCCCACACC AGATTCATCT CGGGGAGCTG CACGCTATCC TGAGGAGGCA GGAGGACTTC TACCCTTTCC TCAAGGATAA CCGCGAGAAG ATCGAGAAGA TTCTGACTTT CAGGATCCCG TACTACGTCG GCCCACTCGC TAGGGGCAAC TCCCGCTTCG CTTGGATGAC CCGCAAGTCA GAGGAGACGA TCACGCCGTG GAACTTCGAG GAGGTGGTCG ACAAGGGCGC TAGCGCTCAG TCGTTCATCG AGAGGATGAC GAATTTCGAC AAGAACCTGC CAAATGAGAA GGTGCTCCCT AAGCACTCGC TCCTGTACGA GTACTTCACA GTCTACAACG AGCTGACTAA GGTGAAGTAT GTGACCGAGG GCATGAGGAA GCCGGCTTTC CTGTCTGGGG AGCAGAAGAA GGCCATCGTG GACCTCCTGT TCAAGACCAA CCGGAAGGTC ACGGTTAAGC AGCTCAAGGA GGACTACTTC AAGAAGATTG AGTGCTTCGA TTCGGTCGAG ATCTCTGGCG TTGAGGACCG CTTCAACGCC TCCCTGGGGA CCTACCACGA TCTCCTGAAG ATCATTAAGG ATAAGGACTT CCTGGACAAC GAGGAGAATG AGGATATCCT CGAGGACATT GTGCTGACAC TCACTCTGTT CGAGGACCGG GAGATGATCG AGGAGCGCCT GAAGACTTAC GCCCATCTCT TCGATGACAA GGTCATGAAG CAGCTCAAGA GGAGGAGGTA CACCGGCTGG GGGAGGCTGA GCAGGAAGCT CATCAACGGC ATTCGGGACA AGCAGTCCGG GAAGACGATC CTCGACTTCC TGAAGAGCGA TGGCTTCGCG AACCGCAATT TCATGCAGCT GATTCACGAT GACAGCCTCA CATTCAAGGA GGATATCCAG AAGGCTCAGG TGAGCGGCCA GGGGGACTCG CTGCACGAGC ATATCGCGAA CCTCGCTGGC TCGCCAGCTA TCAAGAAGGG GATTCTGCAG ACCGTGAAGG TTGTGGACGA GCTGGTGAAG GTCATGGGCA GGCACAAGCC TGAGAACATC GTCATTGAGA TGGCCCGGGA GAATCAGACC ACGCAGAAGG GCCAGAAGAA CTCACGCGAG AGGATGAAGA GGATCGAGGA GGGCATTAAG GAGCTGGGGT CCCAGATCCT CAAGGAGCAC CCGGTGGAGA ACACGCAGCT GCAGAATGAG AAGCTCTACC TGTACTACCT CCAGAATGGC CGCGATATGT ATGTGGACCA GGAGCTGGAT ATTAACAGGC TCAGCGATTA CGACGTCGAT CATATCGTTC CACAGTCATT CCTGAAGGAT GACTCCATTG ACAACAAGGT CCTCACCAGG TCGGACAAGA ACCGGGGCAA GTCTGATAAT GTTCCTTCAG AGGAGGTCGT TAAGAAGATG AAGAACTACT GGCGCCAGCT CCTGAATGCC AAGCTGATCA CGCAGCGGAA GTTCGATAAC CTCACAAAGG CTGAGAGGGG CGGGCTCTCT GAGCTGGACA AGGCGGGCTT CATCAAGAGG CAGCTGGTCG AGACACGGCA GATCACTAAG CACGTTGCGC AGATTCTCGA CTCACGGATG AACACTAAGT ACGATGAGAA TGACAAGCTG ATCCGCGAGG TGAAGGTCAT CACCCTGAAG TCAAAGCTCG TCTCCGACTT CAGGAAGGAT TTCCAGTTCT ACAAGGTTCG GGAGATCAAC AATTACCACC ATGCCCATGA CGCGTACCTG AACGCGGTGG TCGGCACAGC TCTGATCAAG AAGTACCCAA AGCTCGAGAG CGAGTTCGTG TACGGGGACT ACAAGGTTTA CGATGTGAGG AAGATGATCG CCAAGTCGGA GCAGGAGATT GGCAAGGCTA CCGCCAAGTA CTTCTTCTAC TCTAACATTA TGAATTTCTT CAAGACAGAG ATCACTCTGG CCAATGGCGA GATCCGGAAG CGCCCCCTCA TCGAGACGAA CGGCGAGACG GGGGAGATCG TGTGGGACAA GGGCAGGGAT TTCGCGACCG TCAGGAAGGT TCTCTCCATG CCACAAGTGA ATATCGTCAA GAAGACAGAG GTCCAGACTG GCGGGTTCTC TAAGGAGTCA ATTCTGCCTA AGCGGAACAG CGACAAGCTC ATCGCCCGCA AGAAGGACTG GGATCCGAAG AAGTACGGCG GGTTCGACAG CCCCACTGTG GCCTACTCGG TCCTGGTTGT GGCGAAGGTT GAGAAGGGCA AGTCCAAGAA GCTCAAGAGC GTGAAGGAGC TGCTGGGGAT CACGATTATG GAGCGCTCCA GCTTCGAGAA GAACCCGATC GATTTCCTGG AGGCGAAGGG CTACAAGGAG GTGAAGAAGG ACCTGATCAT TAAGCTCCCC AAGTACTCAC TCTTCGAGCT GGAGAACGGC AGGAAGCGGA TGCTGGCTTC CGCTGGCGAG CTGCAGAAGG GGAACGAGCT GGCTCTGCCG TCCAAGTATG TGAACTTCCT CTACCTGGCC TCCCACTACG AGAAGCTCAA GGGCAGCCCC GAGGACAACG AGCAGAAGCA GCTGTTCGTC GAGCAGCACA AGCATTACCT CGACGAGATC ATTGAGCAGA TTTCCGAGTT CTCCAAGCGC GTGATCCTGG CCGACGCGAA TCTGGATAAG GTCCTCTCCG CGTACAACAA GCACCGCGAC AAGCCAATCA GGGAGCAGGC TGAGAATATC ATTCATCTCT TCACCCTGAC GAACCTCGGC GCCCCTGCTG CTTTCAAGTA CTTCGACACA ACTATCGATC GCAAGAGGTA CACAAGCACT AAGGAGGTCC TGGACGCGAC CCTCATCCAC CAGTCGATTA CCGGCCTCTA CGAGACGCGC ATCGACCTGT CTCAGCTCGG GGGCGACAAG CGGCCAGCGG CGACGAAGAA GGCGGGGCAG GCGAAGAAGA AGAAGTGAGC TCAGGCCTCC ATACAAGTAT TGGGGATCCA CTAGCGAATT TCCCCGATCG TTCAAACATT TGGCAATAAA GTTTCTTAAG ATTGAATCCT GTTGCCGGTC TTGCGATGAT TATCATATAA TTTCTGTTGA ATTACGTTAA GCATGTAATA ATTAACATGT AATGCATGAC GTTATTTATG AGATGGGTTT TTATGATTAG AGTCCCGCAA TTATACATTT AATACGCGAT AGAAAACAAA ATATAGCGCG CAAACTAGGA TAAATTATCG CGCGCGGTGT CATCTATGTT ACTAGATCGG AATTCGAGGC GGTTTGCGTA TTGGCTAGAG CAGCTTGCCA ACATGGTGGA GCACGACACT CTCGTCTACT CCAAGAATAT CAAAGATACA GTCTCAGAAG ACCAAAGGGC TATTGAGACT TTTCAACAAA GGGTAATATC GGGAAACCTC CTCGGATTCC ATTGCCCAGC TATCTGTCAC TTCATCAAAA GGACAGTAGA AAAGGAAGGT GGCACCTACA AATGCCATCA TTGCGATAAA GGAAAGGCTA TCGTTCAAGA TGCCTCTGCC GACAGTGGTC CCAAAGATGG ACCCCCACCC ACGAGGAGCA TCGTGGAAAA AGAAGACGTT CCAACCACGT CTTCAAAGCA AGTGGATTGA TGTGATAACA TGGTGGAGCA CGACACTCTC GTCTACTCCA AGAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCTAT TGAGACTTTT CAACAAAGGG TAATATCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCAAAAGGA CAGTAGAAAA GGAAGGTGGC ACCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GGACACGCTG AAATCACCAG TCTCTCTCTA CAAATCTATC TCTCTCGAGC TTTCGCAGAT CCCGGGGGGC AATGAGATAT GAAAAAGCCT GAACTCACCG CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAGTTTAGC GAGAGCCTGA CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC TGCCCGCTGT TCTACAACCG GTCGCGGAGG CTATGGATGC GATCGCTGCG GCCGATCTTA GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCACG ACTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC GATCCGGAGC CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA CCGATGGCTG TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA GGGCAAAGAA ATAGAGTAGA TGCCGACCGG ATCTGTCGAT CGACAAGCTC GAGTTTCTCC ATAATAATGT GTGAGTAGTT CCCAGATAAG GGAATTAGGG TTCCTATAGG GTTTCGCTCA TGTGTTGAGC ATATAAGAAA CCCTTAGTAT GTATTTGTAT TTGTAAAATA CTTCTATCAA TAAAATTTCT AATTCCTAAA ACCAAAATCC AGTACTAAAA TCCAGATCCC CCGAATTAAT TCGGCGTTAA TTCAGTACAT TAAAAACGTC CGCAATGTGT TATTAAGTTG TCACTAGTCA GGTTAACTCA ATTCGGCGTT AATTCAGTAC ATTAAAAACG TCCGCAATGT GTTATTAAGT TGTCTAAGCG TCAATTTGTT TACACCACAA TATATCCTGC CACCAGCCAG CCAACAGCTC CCCGACCGGC AGCTCGGCAC AAAATCACCA CTCGATACAG GCAGCCCATC AGTCCGGGAC GGCGTCAGCG GGAGAGCCGT TGTAAGGCGG CAGACTTTGC TCATGTTACC GATGCTATTC GGAAGAACGG CAACTAAGCT GCCGGGTTTG AAACACGGAT GATCTCGCGG AGGGTAGCAT GTTGATTGTA ACGATGACAG AGCGTTGCTG CCTGTGATCA CTTAAGTAAC TAACTAACAG GAAGAGTTTG TAGAAACGCA AAAAGGCCAT CCGTCAGGAT GGCCTTCTGC TTAGTTTGAT GCCTGGCAGT TTATGGCGGG CGTCCTGCCC GCCACCCTCC GGGCCGTTGC TTCACAACGT TCAAATCCGC TCCCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA TAAAACGAAA GGCCCAGTCT TCCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG CTTAGTAGTT AGACGTCCCC GAGATCCATG CTAGACCATG AATCCAGAAG CCCGAGAGGT TGCCGCCTTT CGGGCTTTTT CTTTTTCAAA AAAAAAAATT TATAAAACGA TCTGTTGCGG CCGGCCGCCG GGTTGTGGGC AAAGGCGCTC GACGGTGGGC AACCGCTTGC GGTTGTCCAC GGGCGGAGCC GGTGCGCGTA GCGCATTGTC CACAAGCCAA GGGCGACCAA TAATTGATAT ATATATTCAT AATTGAAAAG CTAATTGAAC ATACTACTTG CTGTAACTAC TTGCCGGAGC GAGGGGTGTT TGCAAGCTGT TGATCTGAAA GGGCTATTAG CGTTCTCACG TGCCTTTTTG ATTAGCGATT TCACGTGACC TTATTAGCGA TTTCACGTAC TCCGATTAGC GATTTCACGT ACCCTGATTA GCGATTTCAC GTGGATAGTT TTTGGAGCGG GCCGGAAAGC CCCGTGAATC AAGGCTTTGC GGGGCATTAG CGGTTTCACG TGGATAACTA CCCTCTATCC ACAGGCTTCC GGGGATAAAA AAGCCCGCTC GACGGCGGGC TGTTGGATGG GGATCTAGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACCGGG TCTGACGCTC AGTGGAACGG GGCCCAATCT GAATAATGTT ACAACCAATT AACCAATTCT GATTAGAAAA ACTCATCGAG CATCAAATGA AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT TTTGAAAAAG CCGTTTCTGT AATGAAGGAG AAAACTCACC GAGGCAGTTC CATAGGATGG CAAGATCCTG GTATCGGTCT GCGATTCCGA CTCGTCCAAC ATCAATACAA CCTATTAATT TCCCCTCGTC AAAAATAAGG TTATCAAGTG AGAAATCACC ATGAGTGACG ACTGAATCCG GTGAGAATGG CAAAAGTTTA TGCATTTCTT TCCAGACTTG TTCAACAGGC CAGCCATTAC GCTCGTCATC AAAATCACTC GCATCAACCA AACCGTTATT CATTCGTGAT TGCGCCTGAG CGAGACGAAA TACGCGATCG CTGTTAAAAG GACAATTACA AACAGGAATC GAATGCAACC GGCGCAGGGA CACTGCCAGC GCATCAACAA TATTTTCACC TGAATCAGGA TATTCTTCTA ATACCTGGAA TGCTGTTTTT CCGGGGATCG CAGTGGTGAG TAACCATGCA TCATCAGGAG TACGGATAAA ATGCTTGATG GTCGGAAGAG GCATAAATTC CGTCAGCCAG TTTAGTCTGA CCATCTCATC TGTAACATCA TTGGCAACGC TACCTTTGCC ATGTTTCAGA AACAACTCTG GCGCATCGGG CTTCCCATAC AAGCGATAGA TTGTCGCACC TGATTGCCCG ACATTATCGC GAGCCCATTT ATACCCATAT AAATCAGCAT CCATGTTGGA ATTTAATCGC GGCCTCGACG TTTCCCGTTG AATATGGCTC ATAACACCCC TTGTATTACT GTTTATGTAA GCAGACAGTT TTATTGTTCA TGATGATATA TTTTTATCTT GTGCAATGTA ACATCAGAGA TTTTGAGACA CGGGCCAGAG CTGCAGTTTG ATCCCGAGGG GAACCCTGTG GTTGACATGC ACATACAAAT GGACGAACGG ATAAACCTTT TCACGCCCTT TTAAATATCC GTTATTCTAA TAAACGCTCT TTTCTCTTAG
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