PYCm-PK9-T82A
$84.00
In stock
SKU
PL55849
100μg liquid plasmid(Instantly):$105
| name | PYCm-PK9-T82A | another name | aPYCm-PK9-T82A |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCACTATCCA ACTACACTCA TAATGCTTAA AGAAAATTGG CATTATTTTA CCAATTTTTT TAAAACAAAA AAAGAATAAA ATATTTTCTT ATTATTTAGC TAAAATTGTA CTAAATTTTA TACCAAAAAA AATATATATA AATATATGTA ATATATAATA TATATATATA TATATGTATA TATGTATGTA TGTATATATG TATGTATGTA TATATGTATG TATGTATATA TGTATGTATG TATGTATAGT TTATATTTAT TTGGCAAGTT TTTCTGTAAA AATGTTTAAT TAAAAAAAAA AATATATAAC ATGCCAATAT GCACTATTCA TTTTATGGAG GCAATTTTAT GAGTAACCAT TTTTACTTTT TTTTTACATA TGCGCATACT TCGAGTTATA CAATGTTATT GGAGACGCGT AGAGGCCTCG TCTCAGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTTGTTC CTTTTGTTGT AGACATATAA TTGATCATTT TATTTTTTTA TTTATTATTT TCCAAATTCT TTAAATTTTT ATTACCCTTA AAAAATTTCC CATTTTTCCA TATAACCAAA CTTAGCTAGT TATGGCTAGT TTTGTATGAC CAATATTTTT TAATATTCCT AAGAATTTTT TTTTTTAATT TGTTTATAAA ATTATGGAAA ATCCAAGAAA TCACAATTAT CAATTTTTTA GTTAATTTCA CTTTTTATGT TTTCAATAAT TCCATCGAAA AGACACGAAA TGATATACTC CCTTGCACTT CACAAAAGTG CGAAATAAAA AATGTATAGT AAATAATATA ATATTAAATA CGTAGTATTT TTAGGCTTTC CTATATTAAA TTAATTATTC TCGACATATT TATTAATATA TAGTAAGAAA TGTAAATTTT CCAACAAGCA TATAACAATT ACTGTTCATT CAATATTGCC ATCTAAAATG GAATGGAATA TATTTAAAGT GGCTTCATAG AAAGGGGATA TTATTAAAGG TATTATTTTT AACTTTTTTT TTTTTATTTA ATTTTTAAAA TTTATATTTT AGTGCACTAT ATCTATTCGT GAAACATCGG AGAAAATATA TATATTCATA TATTTAGATG CACGTGGATG CATATAAACG TATTTACACA CACCCTTTCG TGTGAACTCT GATTATTGAT TCTATGAATT GAATCATATC AAGATGTATC TAGGGAGTGT CAAAAATGTG GAGGATTTGT TCCTGTCGAA GGGAGAATAC AAACAAGTAT TCCTTTGTTT AAAAAAAAAT GAAAATAGTG AGGCAATACA AATAGATATA AGCTGTGATA GCAACATTTA TAGTATCTTA CAATTATGCA GAGAAAAATA TGGAATCCAT GGTGATTATT TAACAGATAG TTGTGGAAAT ATTGTAGAAA ATATTAAAGA GATAATAGAT GGTGATGCTT TATATTTAAA AGATAATAAA GATAAATTTC ATTTTTTAAA TCAAATAAAA TCAAATAATT TAATAGTAAA TGATTATATA GTAGAAAAAA GAATAGGGTC AGGTGGATTT GGTATAGTAT TTCAAGGTAT ACATATACAA ACTAAACAAA AAGTTGCACT AAAATTTATA CCTAAAAGTA ATTTTTTAGA TGTTACAGAT GTACATAGAG TCTTTATAGA AATACAAACA TTAAGAGGTT TGATACATAA AAATATAATT AAAATGTATG ATGTTAATCA TTTTCAAAAT TATGTATGTC TAATTATGGA ATATGCTGTT AATAGTGATT TAAAAAAATA TATACAAAAA AATAATGGAT ATTTATCTGA AAAAGAAACA TATTTTTTAT TTTTGCAAAT TGTTAAAGGA GTATATTATT GTCATTCTAA GCATATTGTA CATAGAGATT TAAAATTAGA AAATATTTTA CTTGATGAAA ATATGACATG TAAAATTGCA GATTTTGGAT TATCTGACTT TGTTAATGTT GATCAAAATA TTAAAACTGA AGCTGGAAGA ATTCCTTAAG TTTACAATTT AATATTCATA CTTTAAGTAT TTTTTGTAGT ATCCTAGATA TTGTGCTTTA AATGCTCACC CCTCAAAGCA CCAGTAATAT TTTCATCCAC TGAAATACCA TTAAATTTTC AAAAAAATAC TATGCATATA ATGTTATACA TATAAACATA AAACGCCATG TAAATCAAAA AATATATAAA AATATGTATA AAAATAAATA TGCACTAAAT ATAAGCTAAT TATGCATAAA AATTAAAGTG CCCTTTATTA ACTAGTCGTA ATTATTTATA TTTCTATGTT ATAAAAAAAT CCTCATATAA TAATATAATT AATATATGTA ATGTTTTTTT TATTTTATAA TTTTAATATA AAATAATATG TAAATTAATT CAAAAAATAA ATATAATTGT TGTGAAACAA AAAACGTAAT TTTTTCATTT GCCTTCAAAA TTTAAATTTA TTTTAATATT TCCTAAAATA TATATACTTT GTGTATAAAT ATATAAAAAT ATATATTTGC TTATAAATAA ATAAAAAATT TTATAAAACA TAGGGGGATC TATGGTTGGT TCGCTAAACT GCATCGTCGC TGTGTCCCAG AACATGGGCA TCGGCAAGAA CGGGGACCTG CCCTGGCCAC CGCTCAGGAA CGAATTTAGA TATTTCCAGA GAATGACCAC AACCTCTTCA GTAGAAGGTA AACAGAATCT GGTGATTATG GGTAAGAAGA CCTGGTTCTC CATTCCTGAG AAGAATCGAC CTTTAAAGGG TAGAATTAAT TTAGTTCTCA GCAGAGAACT CAAGGAACCT CCACAAGGAG CTCATTTTCT TTCCAGAAGT CTAGATGATG CCTTAAAACT TACTGAACAA CCAGAATTAG CAAATAAAGT AGACATGGTC TGGATAGTTG GTGGCAGTTC TGTTTATAAG GAAGCCATGA ATCACCCAGG CCATCTTAAA CTATTTGTGA CAAGGATCAT GCAAGACTTT GAAAGTGACA CGTTTTTTCC AGAAATTGAT TTGGAGAAAT ATAAACTTCT GCCAGAATAC CCAGGTGTTC TCTCTGATGT CCAGGAGGAG AAAGGCATTA AGTACAAATT TGAAGTATAT GAGAAGAATG ATGCAAGCGG AGGAGGTGGA TCTGGTGGAG GTGGAAGTGC AAGCGTGACA GGGGGAATGG CTAGCAAGTG GGATCAGAAG GGTATGGACA TTGCCTATGA GGAGGCGGCC TTAGGTTACA AAGAGGGTGG TGTTCCTATT GGCGGATGTC TTATCAATAA CAAAGACGGA AGTGTTCTCG GTCGTGGTCA CAACATGAGA TTTCAAAAGG GATCCGCCAC ACTACATGGT GAGATCTCCA CTTTGGAAAA CTGTGGGAGA TTAGAGGGCA AAGTGTACAA AGATACCACT TTGTATACGA CGCTGTCTCC ATGCGACATG TGTACAGGTG CCATCATCAT GTATGGTATT CCACGCTGTG TTGTCGGTGA GAACGTTAAT TTCAAAAGTA AGGGCGAGAA ATATTTACAA ACTAGAGGTC ACGAGGTTGT TGTTGTTGAC GATGAGAGGT GTAAAAAGAT CATGAAACAA TTTATCGATG AAAGACCTCA GGATTGGTTT GAAGATATTG GTGAGGCTTC GGAACCATTT AAGAACGTCT ACTTGCTACC TCAAACAAAC CAATTGCTGG GTTTGTACAC CATCATCAGA AATAAGAATA CAACTAGACC TGATTTCATT TTCTACTCCG ATAGAATCAT CAGATTGTTG GTTGAAGAAG GTTTGAACCA TCTACCTGTG CAAAAGCAAA TTGTGGAAAC TGACACCAAC GAAAACTTCG AAGGTGTCTC ATTCATGGGT AAAATCTGTG GTGTTTCCAT TGTCAGAGCT GGTGAATCGA TGGAGCAAGG ATTAAGAGAC TGTTGTAGGT CTGTGCGTAT CGGTAAAATT TTAATTCAAA GGGACGAGGA GACTGCTTTA CCAAAGTTAT TCTACGAAAA ATTACCAGAG GATATATCTG AAAGGTATGT CTTCCTATTA GACCCAATGC TGGCCACCGG TGGTAGTGCT ATCATGGCTA CAGAAGTCTT GATTAAGAGA GGTGTTAAGC CAGAGAGAAT TTACTTCTTA AACCTAATCT GTAGTAAGGA AGGGATTGAA AAATACCATG CCGCCTTCCC AGAGGTCAGA ATTGTTACTG GTGCCCTCGA CAGAGGTCTA GATGAAAACA AGTATCTAGT TCCAGGGTTG GGTGACTTTG GTGACAGATA CTACTGTGTT GGTTCGGGAG AGGGCAGAGG ATCCCTGCTA ACATGCGGTG ATGTCGAGGA GAATCCTGGC CCAGAATCGA TGGACTATAA GGACCACGAC GGAGACTACA AGGATCATGA TATTGATTAC AAAGACGATG ACGATAAGAT GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC CCAGCAGCCG ACAAGAAGTA CAGCATCGGC CTGGACATCG GCACCAACTC TGTGGGCTGG GCCGTGATCA CCGACGAGTA CAAGGTGCCC AGCAAGAAAT TCAAGGTGCT GGGCAACACC GACCGGCACA GCATCAAGAA GAACCTGATC GGAGCCCTGC TGTTCGACAG CGGCGAAACA GCCGAGGCCA CCCGGCTGAA GAGAACCGCC AGAAGAAGAT ACACCAGACG GAAGAACCGG ATCTGCTATC TGCAAGAGAT CTTCAGCAAC GAGATGGCCA AGGTGGACGA CAGCTTCTTC CACAGACTGG AAGAGTCCTT CCTGGTGGAA GAGGATAAGA AGCACGAGCG GCACCCCATC TTCGGCAACA TCGTGGACGA GGTGGCCTAC CACGAGAAGT ACCCCACCAT CTACCACCTG AGAAAGAAAC TGGTGGACAG CACCGACAAG GCCGACCTGC GGCTGATCTA TCTGGCCCTG GCCCACATGA TCAAGTTCCG GGGCCACTTC CTGATCGAGG GCGACCTGAA CCCCGACAAC AGCGACGTGG ACAAGCTGTT CATCCAGCTG GTGCAGACCT ACAACCAGCT GTTCGAGGAA AACCCCATCA ACGCCAGCGG CGTGGACGCC AAGGCCATCC TGTCTGCCAG ACTGAGCAAG AGCAGACGGC TGGAAAATCT GATCGCCCAG CTGCCCGGCG AGAAGAAGAA TGGCCTGTTC GGAAACCTGA TTGCCCTGAG CCTGGGCCTG ACCCCCAACT TCAAGAGCAA CTTCGACCTG GCCGAGGATG CCAAACTGCA GCTGAGCAAG GACACCTACG ACGACGACCT GGACAACCTG CTGGCCCAGA TCGGCGACCA GTACGCCGAC CTGTTTCTGG CCGCCAAGAA CCTGTCCGAC GCCATCCTGC TGAGCGACAT CCTGAGAGTG AACACCGAGA TCACCAAGGC CCCCCTGAGC GCCTCTATGA TCAAGAGATA CGACGAGCAC CACCAGGACC TGACCCTGCT GAAAGCTCTC GTGCGGCAGC AGCTGCCTGA GAAGTACAAA GAGATTTTCT TCGACCAGAG CAAGAACGGC TACGCCGGCT ACATTGACGG CGGAGCCAGC CAGGAAGAGT TCTACAAGTT CATCAAGCCC ATCCTGGAAA AGATGGACGG CACCGAGGAA CTGCTCGTGA AGCTGAACAG AGAGGACCTG CTGCGGAAGC AGCGGACCTT CGACAACGGC AGCATCCCCC ACCAGATCCA CCTGGGAGAG CTGCACGCCA TTCTGCGGCG GCAGGAAGAT TTTTACCCAT TCCTGAAGGA CAACCGGGAA AAGATCGAGA AGATCCTGAC CTTCCGCATC CCCTACTACG TGGGCCCTCT GGCCAGGGGA AACAGCAGAT TCGCCTGGAT GACCAGAAAG AGCGAGGAAA CCATCACCCC CTGGAACTTC GAGGAAGTGG TGGACAAGGG CGCTTCCGCC CAGAGCTTCA TCGAGCGGAT GACCAACTTC GATAAGAACC TGCCCAACGA GAAGGTGCTG CCCAAGCACA GCCTGCTGTA CGAGTACTTC ACCGTGTATA ACGAGCTGAC CAAAGTGAAA TACGTGACCG AGGGAATGAG AAAGCCCGCC TTCCTGAGCG GCGAGCAGAA AAAGGCCATC GTGGACCTGC TGTTCAAGAC CAACCGGAAA GTGACCGTGA AGCAGCTGAA AGAGGACTAC TTCAAGAAAA TCGAGTGCTT CGACTCCGTG GAAATCTCCG GCGTGGAAGA TCGGTTCAAC GCCTCCCTGG GCACATACCA CGATCTGCTG AAAATTATCA AGGACAAGGA CTTCCTGGAC AATGAGGAAA ACGAGGACAT TCTGGAAGAT ATCGTGCTGA CCCTGACACT GTTTGAGGAC AGAGAGATGA TCGAGGAACG GCTGAAAACC TATGCCCACC TGTTCGACGA CAAAGTGATG AAGCAGCTGA AGCGGCGGAG ATACACCGGC TGGGGCAGGC TGAGCCGGAA GCTGATCAAC GGCATCCGGG ACAAGCAGTC CGGCAAGACA ATCCTGGATT TCCTGAAGTC CGACGGCTTC GCCAACAGAA ACTTCATGCA GCTGATCCAC GACGACAGCC TGACCTTTAA AGAGGACATC CAGAAAGCCC AGGTGTCCGG CCAGGGCGAT AGCCTGCACG AGCACATTGC CAATCTGGCC GGCAGCCCCG CCATTAAGAA GGGCATCCTG CAGACAGTGA AGGTGGTGGA CGAGCTCGTG AAAGTGATGG GCCGGCACAA GCCCGAGAAC ATCGTGATCG AAATGGCCAG AGAGAACCAG ACCACCCAGA AGGGACAGAA GAACAGCCGC GAGAGAATGA AGCGGATCGA AGAGGGCATC AAAGAGCTGG GCAGCCAGAT CCTGAAAGAA CACCCCGTGG AAAACACCCA GCTGCAGAAC GAGAAGCTGT ACCTGTACTA CCTGCAGAAT GGGCGGGATA TGTACGTGGA CCAGGAACTG GACATCAACC GGCTGTCCGA CTACGATGTG GACCATATCG TGCCTCAGAG CTTTCTGAAG GACGACTCCA TCGACAACAA GGTGCTGACC AGAAGCGACA AGAACCGGGG CAAGAGCGAC AACGTGCCCT CCGAAGAGGT CGTGAAGAAG ATGAAGAACT ACTGGCGGCA GCTGCTGAAC GCCAAGCTGA TTACCCAGAG AAAGTTCGAC AATCTGACCA AGGCCGAGAG AGGCGGCCTG AGCGAACTGG ATAAGGCCGG CTTCATCAAG AGACAGCTGG TGGAAACCCG GCAGATCACA AAGCACGTGG CACAGATCCT GGACTCCCGG ATGAACACTA AGTACGACGA GAATGACAAG CTGATCCGGG AAGTGAAAGT GATCACCCTG AAGTCCAAGC TGGTGTCCGA TTTCCGGAAG GATTTCCAGT TTTACAAAGT GCGCGAGATC AACAACTACC ACCACGCCCA CGACGCCTAC CTGAACGCCG TCGTGGGAAC CGCCCTGATC AAAAAGTACC CTAAGCTGGA AAGCGAGTTC GTGTACGGCG ACTACAAGGT GTACGACGTG CGGAAGATGA TCGCCAAGAG CGAGCAGGAA ATCGGCAAGG CTACCGCCAA GTACTTCTTC TACAGCAACA TCATGAACTT TTTCAAGACC GAGATTACCC TGGCCAACGG CGAGATCCGG AAGCGGCCTC TGATCGAGAC AAACGGCGAA ACCGGGGAGA TCGTGTGGGA TAAGGGCCGG GATTTTGCCA CCGTGCGGAA AGTGCTGAGC ATGCCCCAAG TGAATATCGT GAAAAAGACC GAGGTGCAGA CAGGCGGCTT CAGCAAAGAG TCTATCCTGC CCAAGAGGAA CAGCGATAAG CTGATCGCCA GAAAGAAGGA CTGGGACCCT AAGAAGTACG GCGGCTTCGA CAGCCCCACC GTGGCCTATT CTGTGCTGGT GGTGGCCAAA GTGGAAAAGG GCAAGTCCAA GAAACTGAAG AGTGTGAAAG AGCTGCTGGG GATCACCATC ATGGAAAGAA GCAGCTTCGA GAAGAATCCC ATCGACTTTC TGGAAGCCAA GGGCTACAAA GAAGTGAAAA AGGACCTGAT CATCAAGCTG CCTAAGTACT CCCTGTTCGA GCTGGAAAAC GGCCGGAAGA GAATGCTGGC CTCTGCCGGC GAACTGCAGA AGGGAAACGA ACTGGCCCTG CCCTCCAAAT ATGTGAACTT CCTGTACCTG GCCAGCCACT ATGAGAAGCT GAAGGGCTCC CCCGAGGATA ATGAGCAGAA ACAGCTGTTT GTGGAACAGC ACAAGCACTA CCTGGACGAG ATCATCGAGC AGATCAGCGA GTTCTCCAAG AGAGTGATCC TGGCCGACGC TAATCTGGAC AAAGTGCTGT CCGCCTACAA CAAGCACCGG GATAAGCCCA TCAGAGAGCA GGCCGAGAAT ATCATCCACC TGTTTACCCT GACCAATCTG GGAGCCCCTG CCGCCTTCAA GTACTTTGAC ACCACCATCG ACCGGAAGAG GTACACCAGC ACCAAAGAGG TGCTGGACGC CACCCTGATC CACCAGAGCA TCACCGGCCT GTACGAGACA CGGATCGACC TGTCTCAGCT GGGAGGCGAC AAAAGGCCGG CGGCCACGAA AAAGGCCGGC CAGGCAAAAA AGAAAAAGTA AGAATTACCG GTGATCCCGT TTTTCTTACT TATATATTTA TACCAATTGA TTGTATTTAT AACTGTAAAA ATGTGTATGT TGTGTGCATA TTTTTTTTTG TGCATGCACA TGCATGTAAA TAGCTAAAAT TATGAACATT TTATTTTTTG TTCAGAAAAA AAAAACTTTA CACACATAAA ATGGCTAGTA TGAATAGCCA TATTTTATAT AAATTAAATC CTATGAATTT ATGACCATAT TAAAAATTTA GATATTTATG GAACATAATA TGTTTGAAAC AATAAGACAA AATTATTATT ATTATTATTA TTTTTACTGT TATAATTATG TTGTCTCTTC AATGATTCAT AAATAGTTGG ACTTGATTTT TAAAATGTTT ATAATATGAT TAGCATAGTT AAATAAAAAA AGTTGAAAAA TTAAAAAAAA ACATATAAAC ACAAATGATG TTTTTTCCTT CAATTTCGG
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