PYCm-PK9-T265A
$84.00
In stock
SKU
PL55844
100μg liquid plasmid(Instantly):$105
| name | PYCm-PK9-T265A | another name | aPYCm-PK9-T265A |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCACTATCCA ACTACACTCA TAATGCTTAA AGAAAATTGG CATTATTTTA CCAATTTTTT TAAAACAAAA AAAGAATAAA ATATTTTCTT ATTATTTAGC TAAAATTGTA CTAAATTTTA TACCAAAAAA AATATATATA AATATATGTA ATATATAATA TATATATATA TATATGTATA TATGTATGTA TGTATATATG TATGTATGTA TATATGTATG TATGTATATA TGTATGTATG TATGTATAGT TTATATTTAT TTGGCAAGTT TTTCTGTAAA AATGTTTAAT TAAAAAAAAA AATATATAAC ATGCCAATAT GCACTATTCA TTTTATGGAG GCAATTTTAT GAGTAACCAT TTTTACTTTT TTTTTACATA TGCGCATACT TCGAGTTATA CAATGTTATT GGAGACGCGT AGAGGCCTCG TCTCAGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTTGTTC CTTTTGTTGT AGACATATAA TTGATCATTT TATTTTTTTA TTTATTATTT TCCAAATTCT TTAAATTTTT ATTACCCTTA AAAAATTTCC CATTTTTCCA TATAACCAAA CTTAGCTAGT TATGGCTAGT TTTGTATGAC CAATATTTTT TAATATTCCT AAGAATTTTT TTTTTTAATT TGTTTATAAA ATTATGGAAA ATCCAAGAAA TCACAATTAT CAATTTTTTA GTTAATTTCA CTTTTTATGT TTTCAATAAT TCCATCGAAA AGACACGAAA TGATATACTC CCTTGCACTT CACAAAAGTG CGAAATAAAA AATGTATAGT AAATAATATA ATATTAAATA CGTAGTATTT TTAGGCTTTC CTATATTAAA TTAATTATTC TCGACATATT TATTAATATA TAGTAAGAAA TGTAAATTTT CCAACAAGCA TATAACAATT ACTGTTCATT CAATATTGCC ATCTAAAATG GAATGGAATA TATTTAAAGT GGCTTCATAG AAAGGGGATA TTATTAAAGG TATTATTTTT AACTTTTTTT TTTTTATTTA ATTTTTAAAA TTTATATTTT AGTGCACTAT ATCTATTCGT GAAACATCGG AGAAAATATA TATATTCATA TATTTAGATG CACGTGGATG CATATAAACG TATTTACACA CACCCTTTCG TGTGAACTCT GATTATTGAT TCTATGAATT GAATCATATC AAGATGTATC TAGGGAGTGT CAAAAATGTG GAGGATTTGT TCCTGTCGAA GGGAGAATAC AAACAAGTAT TCCTTTGTTT AAAAAAAAAT GAAAATAGTG AGGCAATACA AATAGATATA AGCTGTGATA GCAACATTTA TAGTATCTTA CAATTATGCA GAGAAAAATA TGGAATCCAT GGTGATTATT TAACAGATAG TTGTGGAAAT ATTGTAGAAA ATATTAAAGA GATAATAGAT GGTGATACTT TATATTTAAA AGATAATAAA GATAAATTTC ATTTTTTAAA TCAAATAAAA TCAAATAATT TAATAGTAAA TGATTATATA GTAGAAAAAA GAATAGGGTC AGGTGGATTT GGTATAGTAT TTCAAGGTAT ACATATACAA ACTAAACAAA AAGTTGCACT AAAATTTATA CCTAAAAGTA ATTTTTTAGA TGTTACAGAT GTACATAGAG TCTTTATAGA AATACAAACA TTAAGAGGTT TGATACATAA AAATATAATT AAAATGTATG ATGTTAATCA TTTTCAAAAT TATGTATGTC TAATTATGGA ATATGCTGTT AATAGTGATT TAAAAAAATA TATACAAAAA AATAATGGAT ATTTATCTGA AAAAGAAACA TATTTTTTAT TTTTGCAAAT TGTTAAAGGA GTATATTATT GTCATTCTAA GCATATTGTA CATAGAGATT TAAAATTAGA AAATATTTTA CTTGATGAAA ATATGACATG TAAAATTGCA GATTTTGGAT TATCTGACTT TGTTAATGTT GATCAAAATA TTAAAGCTGA AGCTGGAACA GAATTCCTTA AGTTTACAAT TTAATATTCA TACTTTAAGT ATTTTTTGTA GTATCCTAGA TATTGTGCTT TAAATGCTCA CCCCTCAAAG CACCAGTAAT ATTTTCATCC ACTGAAATAC CATTAAATTT TCAAAAAAAT ACTATGCATA TAATGTTATA CATATAAACA TAAAACGCCA TGTAAATCAA AAAATATATA AAAATATGTA TAAAAATAAA TATGCACTAA ATATAAGCTA ATTATGCATA AAAATTAAAG TGCCCTTTAT TAACTAGTCG TAATTATTTA TATTTCTATG TTATAAAAAA ATCCTCATAT AATAATATAA TTAATATATG TAATGTTTTT TTTATTTTAT AATTTTAATA TAAAATAATA TGTAAATTAA TTCAAAAAAT AAATATAATT GTTGTGAAAC AAAAAACGTA ATTTTTTCAT TTGCCTTCAA AATTTAAATT TATTTTAATA TTTCCTAAAA TATATATACT TTGTGTATAA ATATATAAAA ATATATATTT GCTTATAAAT AAATAAAAAA TTTTATAAAA CATAGGGGGA TCTATGGTTG GTTCGCTAAA CTGCATCGTC GCTGTGTCCC AGAACATGGG CATCGGCAAG AACGGGGACC TGCCCTGGCC ACCGCTCAGG AACGAATTTA GATATTTCCA GAGAATGACC ACAACCTCTT CAGTAGAAGG TAAACAGAAT CTGGTGATTA TGGGTAAGAA GACCTGGTTC TCCATTCCTG AGAAGAATCG ACCTTTAAAG GGTAGAATTA ATTTAGTTCT CAGCAGAGAA CTCAAGGAAC CTCCACAAGG AGCTCATTTT CTTTCCAGAA GTCTAGATGA TGCCTTAAAA CTTACTGAAC AACCAGAATT AGCAAATAAA GTAGACATGG TCTGGATAGT TGGTGGCAGT TCTGTTTATA AGGAAGCCAT GAATCACCCA GGCCATCTTA AACTATTTGT GACAAGGATC ATGCAAGACT TTGAAAGTGA CACGTTTTTT CCAGAAATTG ATTTGGAGAA ATATAAACTT CTGCCAGAAT ACCCAGGTGT TCTCTCTGAT GTCCAGGAGG AGAAAGGCAT TAAGTACAAA TTTGAAGTAT ATGAGAAGAA TGATGCAAGC GGAGGAGGTG GATCTGGTGG AGGTGGAAGT GCAAGCGTGA CAGGGGGAAT GGCTAGCAAG TGGGATCAGA AGGGTATGGA CATTGCCTAT GAGGAGGCGG CCTTAGGTTA CAAAGAGGGT GGTGTTCCTA TTGGCGGATG TCTTATCAAT AACAAAGACG GAAGTGTTCT CGGTCGTGGT CACAACATGA GATTTCAAAA GGGATCCGCC ACACTACATG GTGAGATCTC CACTTTGGAA AACTGTGGGA GATTAGAGGG CAAAGTGTAC AAAGATACCA CTTTGTATAC GACGCTGTCT CCATGCGACA TGTGTACAGG TGCCATCATC ATGTATGGTA TTCCACGCTG TGTTGTCGGT GAGAACGTTA ATTTCAAAAG TAAGGGCGAG AAATATTTAC AAACTAGAGG TCACGAGGTT GTTGTTGTTG ACGATGAGAG GTGTAAAAAG ATCATGAAAC AATTTATCGA TGAAAGACCT CAGGATTGGT TTGAAGATAT TGGTGAGGCT TCGGAACCAT TTAAGAACGT CTACTTGCTA CCTCAAACAA ACCAATTGCT GGGTTTGTAC ACCATCATCA GAAATAAGAA TACAACTAGA CCTGATTTCA TTTTCTACTC CGATAGAATC ATCAGATTGT TGGTTGAAGA AGGTTTGAAC CATCTACCTG TGCAAAAGCA AATTGTGGAA ACTGACACCA ACGAAAACTT CGAAGGTGTC TCATTCATGG GTAAAATCTG TGGTGTTTCC ATTGTCAGAG CTGGTGAATC GATGGAGCAA GGATTAAGAG ACTGTTGTAG GTCTGTGCGT ATCGGTAAAA TTTTAATTCA AAGGGACGAG GAGACTGCTT TACCAAAGTT ATTCTACGAA AAATTACCAG AGGATATATC TGAAAGGTAT GTCTTCCTAT TAGACCCAAT GCTGGCCACC GGTGGTAGTG CTATCATGGC TACAGAAGTC TTGATTAAGA GAGGTGTTAA GCCAGAGAGA ATTTACTTCT TAAACCTAAT CTGTAGTAAG GAAGGGATTG AAAAATACCA TGCCGCCTTC CCAGAGGTCA GAATTGTTAC TGGTGCCCTC GACAGAGGTC TAGATGAAAA CAAGTATCTA GTTCCAGGGT TGGGTGACTT TGGTGACAGA TACTACTGTG TTGGTTCGGG AGAGGGCAGA GGATCCCTGC TAACATGCGG TGATGTCGAG GAGAATCCTG GCCCAGAATC GATGGACTAT AAGGACCACG ACGGAGACTA CAAGGATCAT GATATTGATT ACAAAGACGA TGACGATAAG ATGGCCCCAA AGAAGAAGCG GAAGGTCGGT ATCCACGGAG TCCCAGCAGC CGACAAGAAG TACAGCATCG GCCTGGACAT CGGCACCAAC TCTGTGGGCT GGGCCGTGAT CACCGACGAG TACAAGGTGC CCAGCAAGAA ATTCAAGGTG CTGGGCAACA CCGACCGGCA CAGCATCAAG AAGAACCTGA TCGGAGCCCT GCTGTTCGAC AGCGGCGAAA CAGCCGAGGC CACCCGGCTG AAGAGAACCG CCAGAAGAAG ATACACCAGA CGGAAGAACC GGATCTGCTA TCTGCAAGAG ATCTTCAGCA ACGAGATGGC CAAGGTGGAC GACAGCTTCT TCCACAGACT GGAAGAGTCC TTCCTGGTGG AAGAGGATAA GAAGCACGAG CGGCACCCCA TCTTCGGCAA CATCGTGGAC GAGGTGGCCT ACCACGAGAA GTACCCCACC ATCTACCACC TGAGAAAGAA ACTGGTGGAC AGCACCGACA AGGCCGACCT GCGGCTGATC TATCTGGCCC TGGCCCACAT GATCAAGTTC CGGGGCCACT TCCTGATCGA GGGCGACCTG AACCCCGACA ACAGCGACGT GGACAAGCTG TTCATCCAGC TGGTGCAGAC CTACAACCAG CTGTTCGAGG AAAACCCCAT CAACGCCAGC GGCGTGGACG CCAAGGCCAT CCTGTCTGCC AGACTGAGCA AGAGCAGACG GCTGGAAAAT CTGATCGCCC AGCTGCCCGG CGAGAAGAAG AATGGCCTGT TCGGAAACCT GATTGCCCTG AGCCTGGGCC TGACCCCCAA CTTCAAGAGC AACTTCGACC TGGCCGAGGA TGCCAAACTG CAGCTGAGCA AGGACACCTA CGACGACGAC CTGGACAACC TGCTGGCCCA GATCGGCGAC CAGTACGCCG ACCTGTTTCT GGCCGCCAAG AACCTGTCCG ACGCCATCCT GCTGAGCGAC ATCCTGAGAG TGAACACCGA GATCACCAAG GCCCCCCTGA GCGCCTCTAT GATCAAGAGA TACGACGAGC ACCACCAGGA CCTGACCCTG CTGAAAGCTC TCGTGCGGCA GCAGCTGCCT GAGAAGTACA AAGAGATTTT CTTCGACCAG AGCAAGAACG GCTACGCCGG CTACATTGAC GGCGGAGCCA GCCAGGAAGA GTTCTACAAG TTCATCAAGC CCATCCTGGA AAAGATGGAC GGCACCGAGG AACTGCTCGT GAAGCTGAAC AGAGAGGACC TGCTGCGGAA GCAGCGGACC TTCGACAACG GCAGCATCCC CCACCAGATC CACCTGGGAG AGCTGCACGC CATTCTGCGG CGGCAGGAAG ATTTTTACCC ATTCCTGAAG GACAACCGGG AAAAGATCGA GAAGATCCTG ACCTTCCGCA TCCCCTACTA CGTGGGCCCT CTGGCCAGGG GAAACAGCAG ATTCGCCTGG ATGACCAGAA AGAGCGAGGA AACCATCACC CCCTGGAACT TCGAGGAAGT GGTGGACAAG GGCGCTTCCG CCCAGAGCTT CATCGAGCGG ATGACCAACT TCGATAAGAA CCTGCCCAAC GAGAAGGTGC TGCCCAAGCA CAGCCTGCTG TACGAGTACT TCACCGTGTA TAACGAGCTG ACCAAAGTGA AATACGTGAC CGAGGGAATG AGAAAGCCCG CCTTCCTGAG CGGCGAGCAG AAAAAGGCCA TCGTGGACCT GCTGTTCAAG ACCAACCGGA AAGTGACCGT GAAGCAGCTG AAAGAGGACT ACTTCAAGAA AATCGAGTGC TTCGACTCCG TGGAAATCTC CGGCGTGGAA GATCGGTTCA ACGCCTCCCT GGGCACATAC CACGATCTGC TGAAAATTAT CAAGGACAAG GACTTCCTGG ACAATGAGGA AAACGAGGAC ATTCTGGAAG ATATCGTGCT GACCCTGACA CTGTTTGAGG ACAGAGAGAT GATCGAGGAA CGGCTGAAAA CCTATGCCCA CCTGTTCGAC GACAAAGTGA TGAAGCAGCT GAAGCGGCGG AGATACACCG GCTGGGGCAG GCTGAGCCGG AAGCTGATCA ACGGCATCCG GGACAAGCAG TCCGGCAAGA CAATCCTGGA TTTCCTGAAG TCCGACGGCT TCGCCAACAG AAACTTCATG CAGCTGATCC ACGACGACAG CCTGACCTTT AAAGAGGACA TCCAGAAAGC CCAGGTGTCC GGCCAGGGCG ATAGCCTGCA CGAGCACATT GCCAATCTGG CCGGCAGCCC CGCCATTAAG AAGGGCATCC TGCAGACAGT GAAGGTGGTG GACGAGCTCG TGAAAGTGAT GGGCCGGCAC AAGCCCGAGA ACATCGTGAT CGAAATGGCC AGAGAGAACC AGACCACCCA GAAGGGACAG AAGAACAGCC GCGAGAGAAT GAAGCGGATC GAAGAGGGCA TCAAAGAGCT GGGCAGCCAG ATCCTGAAAG AACACCCCGT GGAAAACACC CAGCTGCAGA ACGAGAAGCT GTACCTGTAC TACCTGCAGA ATGGGCGGGA TATGTACGTG GACCAGGAAC TGGACATCAA CCGGCTGTCC GACTACGATG TGGACCATAT CGTGCCTCAG AGCTTTCTGA AGGACGACTC CATCGACAAC AAGGTGCTGA CCAGAAGCGA CAAGAACCGG GGCAAGAGCG ACAACGTGCC CTCCGAAGAG GTCGTGAAGA AGATGAAGAA CTACTGGCGG CAGCTGCTGA ACGCCAAGCT GATTACCCAG AGAAAGTTCG ACAATCTGAC CAAGGCCGAG AGAGGCGGCC TGAGCGAACT GGATAAGGCC GGCTTCATCA AGAGACAGCT GGTGGAAACC CGGCAGATCA CAAAGCACGT GGCACAGATC CTGGACTCCC GGATGAACAC TAAGTACGAC GAGAATGACA AGCTGATCCG GGAAGTGAAA GTGATCACCC TGAAGTCCAA GCTGGTGTCC GATTTCCGGA AGGATTTCCA GTTTTACAAA GTGCGCGAGA TCAACAACTA CCACCACGCC CACGACGCCT ACCTGAACGC CGTCGTGGGA ACCGCCCTGA TCAAAAAGTA CCCTAAGCTG GAAAGCGAGT TCGTGTACGG CGACTACAAG GTGTACGACG TGCGGAAGAT GATCGCCAAG AGCGAGCAGG AAATCGGCAA GGCTACCGCC AAGTACTTCT TCTACAGCAA CATCATGAAC TTTTTCAAGA CCGAGATTAC CCTGGCCAAC GGCGAGATCC GGAAGCGGCC TCTGATCGAG ACAAACGGCG AAACCGGGGA GATCGTGTGG GATAAGGGCC GGGATTTTGC CACCGTGCGG AAAGTGCTGA GCATGCCCCA AGTGAATATC GTGAAAAAGA CCGAGGTGCA GACAGGCGGC TTCAGCAAAG AGTCTATCCT GCCCAAGAGG AACAGCGATA AGCTGATCGC CAGAAAGAAG GACTGGGACC CTAAGAAGTA CGGCGGCTTC GACAGCCCCA CCGTGGCCTA TTCTGTGCTG GTGGTGGCCA AAGTGGAAAA GGGCAAGTCC AAGAAACTGA AGAGTGTGAA AGAGCTGCTG GGGATCACCA TCATGGAAAG AAGCAGCTTC GAGAAGAATC CCATCGACTT TCTGGAAGCC AAGGGCTACA AAGAAGTGAA AAAGGACCTG ATCATCAAGC TGCCTAAGTA CTCCCTGTTC GAGCTGGAAA ACGGCCGGAA GAGAATGCTG GCCTCTGCCG GCGAACTGCA GAAGGGAAAC GAACTGGCCC TGCCCTCCAA ATATGTGAAC TTCCTGTACC TGGCCAGCCA CTATGAGAAG CTGAAGGGCT CCCCCGAGGA TAATGAGCAG AAACAGCTGT TTGTGGAACA GCACAAGCAC TACCTGGACG AGATCATCGA GCAGATCAGC GAGTTCTCCA AGAGAGTGAT CCTGGCCGAC GCTAATCTGG ACAAAGTGCT GTCCGCCTAC AACAAGCACC GGGATAAGCC CATCAGAGAG CAGGCCGAGA ATATCATCCA CCTGTTTACC CTGACCAATC TGGGAGCCCC TGCCGCCTTC AAGTACTTTG ACACCACCAT CGACCGGAAG AGGTACACCA GCACCAAAGA GGTGCTGGAC GCCACCCTGA TCCACCAGAG CATCACCGGC CTGTACGAGA CACGGATCGA CCTGTCTCAG CTGGGAGGCG ACAAAAGGCC GGCGGCCACG AAAAAGGCCG GCCAGGCAAA AAAGAAAAAG TAAGAATTAC CGGTGATCCC GTTTTTCTTA CTTATATATT TATACCAATT GATTGTATTT ATAACTGTAA AAATGTGTAT GTTGTGTGCA TATTTTTTTT TGTGCATGCA CATGCATGTA AATAGCTAAA ATTATGAACA TTTTATTTTT TGTTCAGAAA AAAAAAACTT TACACACATA AAATGGCTAG TATGAATAGC CATATTTTAT ATAAATTAAA TCCTATGAAT TTATGACCAT ATTAAAAATT TAGATATTTA TGGAACATAA TATGTTTGAA ACAATAAGAC AAAATTATTA TTATTATTAT TATTTTTACT GTTATAATTA TGTTGTCTCT TCAATGATTC ATAAATAGTT GGACTTGATT TTTAAAATGT TTATAATATG ATTAGCATAG TTAAATAAAA AAAGTTGAAA AATTAAAAAA AAACATATAA ACACAAATGA TGTTTTTTCC TTCAATTTCG G
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