pHCMC04-BmSQS-6×His
$113.00
In stock
SKU
PL55834
100μg liquid plasmid(Instantly):$134
| name | pHCMC04-BmSQS-6×His | another name | apHCMC04-BmSQS-6×His |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Chl | Fluorescent labeling | |
| Promoter | xylA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGAGGCCTG CAGGCATGCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACTGCCGGGC CTCTTGCGGG ATCGGCGAGG CTAGTTACCC TTAAGTTATT GGTATGACTG GTTTTAAGCG CAAAAAAAGT TGCTTTTTCG TACCTATTAA TGTATCGTTT TAGAAAACCG ACTGTAAAAA GTACAGTCGG CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT GACAATTCCT GAATAGAGTT CATAAACAAT CCTGCATGAT AACCATCACA AACAGAATGA TGTACCTGTA AAGATAGCGG TAAATATATT GAATTACCTT TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA ATTACTATTA TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG AACCATTATA TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG AAGTCATTCT TTACAGGAGT CCAAATACCA GAGAATGTTT TAGATACACC ATCAAAAATT GTATAAAGTG GCTCTAACTT ATCCCAATAA CCTAACTCTC CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT TATCACCCTT GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG TTTGTTGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT AAATCAATTT TATTAAAGTT CATTTGATAT GCCTCCTAAA TTTTTATCTA AAGTGAATTT AGGAGGCTTA CTTGTCTGCT TTCTTCATTA GAATCAATCC TTTTTTAAAA GTCAATATTA CTGTAACATA AATATATATT TTAAAAATAT CCCACTTTAT CCAATTTTCG TTTGTTGAAC TAATGGGTGC TTTAGTTGAA GAATAAAGAC CACATTAAAA AATGTGGTCT TTTGTGTTTT TTTAAAGGAT TTGAGCGTAG CGAAAAATCC TTTTCTTTCT TATCTTGATA ATAAGGGTAA CTATTGCCGA TCGTCCATTC CGACAGCATC GCCAGTCACT ATGGCGTGCT GCTAGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCCTAA CTTATAGGGG TAACACTTAA AAAAGAATCA ATAACGATAG AAACCGCTCC TAAAGCAGGT GCATTTTTTC CTAACGAAGA AGGCAATAGT TCACATTTAT TGTCTAAATG AGAATGGACT CTAGAAGAAA CTTCGTTTTT AATCGTATTT AAAACAATGG GATGAGATTC AATTATATGA TTTCTCAAGA TAACAGCTTC TATATCAAAT GTATTAAGGA TATTGGTTAA TCCAATTCCG ATATAAAAGC CAAAGTTTTG AAGTGCATTT AACATTTCTA CATCATTTTT ATTTGCGCGT TCCACAATCT CTTTTCGAGA AATATTCTTT TCTTCTTTAG AGAGCGAAGC CAGTAACGCT TTTTCAGAAG CATATAATTC CCAACAGCCT CGATTTCCAC AGCTGCATTT GGGTCCATTA AAATCTATCG TCATATGACC CATTTCCCCA GAAAAACCCT GAACACCTTT ATACAATTCG TTGTTAATAA CAAGTCCAGT TCCAATTCCG ATATTAATAC TGATGTAAAC GATGTTTTCA TAGTTTTTTG TCATACCAAA TACTTTTTCA CCGTATGCTC CTGCATTAGC TTCATTTTCA ACAAAAACCG GAACATTAAA CTCACTCTCA ATTAAAAACT GCAAATCTTT GATATTCCAA TTTAAGTTAG GCATGAAAAT AATTTGCTGA TGACGATCTA CAAGGCCTGG AACACAAATT CCTATTCCGA CTAGACCATA AGGGGACTCA GGCATATGGG TTACAAAACC ATGAATAAGT GCAAATAAAA TCTCTTTTAC TTCACTAGCG GAAGAACTAG ACAAGTCAGA AGTCTTCTCG AGAATAATAT TTCCTTCTAA GTCGGTTAGA ATTCCGTTAA GATAGTCGAC TCCTATATCA ATACCAATCG AGTAGCCTGC ATTCTTATTA AAAACAAGCA TTACAGGTCT TCTGCCGCCT CTAGATTGCC CTGCCCCAAT TTCAAAAATA AAATCTTTTT CAAGCAGTGT ATTTACTTGA GAGGAGACAG TAGACTTGTT TAATCCTGTA ATCTCAGAGA GAGTTGCCCT GGAGACAGGG GAGTTCTTCA AAATTTCATC TAATATTAAT TTTTGATTCA TTTTTTTTAC TAAAGCTTGA TCTGCAATTT GAATAATAAC CACTCCTTTG TTTATCCACC GAACTAAGTT GGTGTTTTTT GAAGCTTGAA TTAGATATTT AAAAGTATCA TATCTAATAT TATAACTAAA TTTTCTAAAA AAAACATTGA AATAAACATT TATTTTGTAT ATGATGAGAT AAAGTTAGTT TATTGGATAA ACAAACTAAC TCAATTAAGA TAGTTGATGG ATAAACTTGT TCACTTAAAT CAAAGGGGGA AATGACAAAT GGTCTAAACT AGTAAGAGGA GGAGAAATAT GTCTGTTCCT AACAAACTTC GTGATAACGC TATGGTTATG CTTAAAGAAA CATCTCGTAC ATTCTTCATC CCTATCTCTC ATCTTCCTGC TGAACTTCAA AACGCTGTTG GCTCTGCTTA CCTTTGCATG CGTGCTATCG ATGAAATCGA AGATCATCCT GAACTTGAAG CTGGCGTTAA ATCTCGTCTT CTTTACGCTA TCTCTGATCT TCTTAAAAAA TCTTTCAACG AAGATGAATA CATGGCTCTT GTTGGCCCTT ACCAAGCTGA TCTTCCTGAT GTTACACTTC AACTTGGCAA CTGGATCGCT CTTTGCCCTA CAGGCGTTGC TGATCAAGTT CTTGATGCTA CATCTATCAT GGCTAAAGGC ATGGCTGATT GGGTTGAAAA AGATTGGCAT ATCCAAAACG AAGCTGATCT TGATGATTAC ACATTCTACG TTGCTGGCCT TGTTGGCGTT ATGCTTAACG ATATCTGGAA ATGGTACGAT GGCACAGAAA CAGATAAAGA ACTTGCTATC GCTTTCGGCC GTGGCCTTCA ATCTGTTAAC ATCCTTCGTA ACACATCTGA AGATTCTGAA CGTGGCGTTT CTTTCTTCCC TAACAACTGG TCTCGTGAAG ATATGTTCGC TTACGCTCGT CGTAACCTTT CTCTTGGCAA AAAATACCTT GAAGATGTTA AATCTCTTCC TATCCTTCAT TTCTGCAAAA TCCCTCTTGC TCTTGCTGAT GGCACACTTA ACGCTCTTAT GAAAGGCAAA GAAAAAATGA CACGTGATGA TGTTAACAAA ACAGTTAACG AAGTTGTTGA TATGCATCAT CATCATCATC ATTAAGGATC CTCTAGAGTC GACGTCCCCG GGGCAGCCCG CCTAATGAGC GGGCTTTTTT CACGTCACGC GTCCATGGAG ATCTTTGTCT GCAACTGAAA AGTTTATACC TTACCTGGAA CAAATGGTTG AAACATACGA GGCTAATATC GGCTTATTAG GAATAGTCCC TGTACTAATA AAATCAGGTG GATCAGTTGA TCAGTATATT TTGGACGAAG CTCGGAAAGA ATTTGGAGAT GACTTGCTTA ATTCCACAAT TAAATTAAGG GAAAGAATAA AGCGATTTGA TGTTCAAGGA ATCACGGAAG AAGATACTCA TGATAAAGAA GCTCTAAAAC TATTCAATAA CCTTACAATG GAATTGATCG AAAGGGTGGA AGGTTAATGG TACGAAAATT AGGGGATCTA CCTAGAAAGC CACAAGGCGA TAGGTCAAGC TTAAAGAACC CTTACATGGA TCTTACAGAT TCTGAAAGTA AAGAAACAAC AGAGGTTAAA CAAACAGAAC CAAAAAGAAA AAAAGCATTG TTGAAAACAA TGAAAGTTGA TGTTTCAATC CATAATAAGA TTAAATCGCT GCACGAAATT CTGGCAGCAT CCGAAGGGAA TTCATATTAC TTAGAGGATA CTATTGAGAG AGCTATTGAT AAGATGGTTG AGACATTACC TGAGAGCCAA AAAACTTTTT ATGAATATGA ATTAAAAAAA AGAACCAACA AAGGCTGAGA CAGACTCCAA ACGAGTCTGT TTTTTTAAAA AAAATATTAG GAGCATTGAA TATATATTAG AGAATTAAGA AAGACATGGG AATAAAAATA TTTTAAATCC AGTAAAAATA TGATAAGATT ATTTCAGAAT ATGAAGAACT CTGTTTGTTT TTGATGAAAA AACAAACAAA AAAAATCCAC CTAACGGAAT CTCAATTTAA CTAACAGCGG CCAAACTGAG AAGTTAAATT TGAGAAGGGG AAAAGGCGGA TTTATACTTG TATTTAACTA TCTCCATTTT AACATTTTAT TAAACCCCAT ACAAGTGAAA ATCCTCTTTT ACACTGTTCC TTTAGGTGAT CGCGGAGGGA CATTATGAGT GAAGTAAACC TAAAAGGAAA TACAGATGAA TTAGTGTATT ATCGACAGCA AACCACTGGA AATAAAATCG CCAGGAAGAG AATCAAAAAA GGGAAAGAAG AAGTTTATTA TGTTGCTGAA ACGGAAGAGA AGATATGGAC AGAAGAGCAA ATAAAAAACT TTTCTTTAGA CAAATTTGGT ACGCATATAC CTTACATAGA AGGTCATTAT ACAATCTTAA ATAATTACTT CTTTGATTTT TGGGGCTATT TTTTAGGTGC TGAAGGAATT GCGCTCTATG CTCACCTAAC TCGTTATGCA TACGGCAGCA AAGACTTTTG CTTTCCTAGT CTACAAACAA TCGCTAAAAA AATGGACAAG ACTCCTGTTA CAGTTAGAGG CTACTTGAAA CTGCTTGAAA GGTACGGTTT TATTTGGAAG GTAAACGTCC GTAATAAAAC CAAGGATAAC ACAGAGGAAT CCCCGATTTT TAAGATTAGA CGTAAGGTTC CTTTGCTTTC AGAAGAACTT TTAAATGGAA ACCCTAATAT TGAAATTCCA GATGACGAGG AAGCACATGT AAAGAAGGCT TTAAAAAAGG AAAAAGAGGG TCTTCCAAAG GTTTTGAAAA AAGAGCACGA TGAATTTGTT AAAAAAATGA TGGATGAGTC AGAAACAATT AATATTCCAG AGGCCTTACA ATATGACACA ATGTATGAAG ATATACTCAG TAAAGGAGAA ATTCGAAAAG AAATCAAAAA ACAAATACCT AATCCTACAA CATCTTTTGA GAGTATATCA ATGACAACTG AAGAGGAAAA AGTCGACAGT ACTTTAAAAA GCGAAATGCA AAATCGTGTC TCTAAGCCTT CTTTTGATAC CTGGTTTAAA AACACTAAGA TCAAAATTGA AAATAAAAAT TGTTTATTAC TTGTACCGAG TGAATTTGCA TTTGAATGGA TTAAGAAAAG ATATTTAGAA ACAATTAAAA CAGTCCTTGA AGAAGCTGGA TATGTTTTCG AAAAAATCGA ACTAAGAAAA GTGCAATAAA CTGCTGAAGT ATTTCAGCAG TTTTTTTTAT TTAGAAATAG TGAAAAAAAT ATAATCAGGG AGGTATCAAT ATTTAATGAG TACTGATTTA AATTTATTTA GACTGGAATT AATAATTAAC ACGTAGACTA ATTAAAATTT AATGAGGGAT AAAGAGGATA CAAAAATATT AATTTCAATC CCTATTAAAT TTTAACAAGG GGGGGATTAA AATTTAATTA GAGGTTTATC CACAAGAAAA GACCCTAATA AAATTTTTAC TAGGGTTATA ACACTGATTA ATTTCTTAAT GGGGGAGGGA TTAAAATTTA ATGACAAAGA AAACAATCTT TTAAGAAAAG CTTTTAAAAG ATAATAATAA AAAGAGCTTT GCGATTAAGC AAAACTCTTT ACTTTTTCAT TGACATTATC AAATTCATCG ATTTCAAATT GTTGTTGTAT CATAAAGTTA ATTCTGTTTT GCACAACCTT TTCAGGAATA TAAAACACAT CTGAGGCTTG TTTTATAAAC TCAGGGTCGC TAAAGTCAAT GTAACGTAGC ATATGATATG GTATAGCTTC CACCCAAGTT AGCCTTTCTG CTTCTTCTGA ATGTTTTTCA TATACTTCCA TGGGTATCTC TAAATGATTT TCCTCATGTA GCAAGGTATG AGCAAAAAGT TTATGGAATT GATAGTTCCT CTCTTTTTCT TCAACTTTTT TATCTAAAAC AAACACTTTA ACATCTGAGT CAATGTAAGC ATAAGATGTT TTTCCAGTCA TAATTTCAAT CCCAAATCTT TTAGACAGAA ATTCTGGACG TAAATCTTTT GGTGAAAGAA TTTTTTTATG TAGCAATATA TCCGATACAG CACCTTCTAA AAGCGTTGGT GAATAGGGCA TTTTACCTAT CTCCTCTCAT TTTGTGGAAT AAAAATAGTC ATATTCGTCC ATCTACCTAT CCTATTATCG AACAGTTGAA CTTTTTAATC AAGGATCAGT CCTTTTTTTC ATTATTCTTA AACTGTGCTC TTAACTTTAA CAACTCGATT TGTTTTTCCA GATC
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