NM_004963.4;DIAR6; GC-C; GUC2C; MECIL; MUCIL; STAR
100μg liquid plasmid(Instantly):$120
| name | PiggyBac-CMV-GUCY2C(human)-EF1a-Puro | another name | aPiggyBac-CMV-GUCY2C(human)-EF1a-Puro |
| Insert Size (bp) | 3222bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | EF1a-R：gagccagtacacgacatcac | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGAAGA CGTTGCTGTT GGACTTGGCT TTGTGGTCAC TGCTCTTCCA GCCCGGGTGG CTGTCCTTTA GTTCCCAGGT GAGTCAGAAC TGCCACAATG GCAGCTATGA AATCAGCGTC CTGATGATGG GCAACTCAGC CTTTGCAGAG CCCCTGAAAA ACTTGGAAGA TGCGGTGAAT GAGGGGCTGG AAATAGTGAG AGGACGTCTG CAAAATGCTG GCCTAAATGT GACTGTGAAC GCTACTTTCA TGTATTCGGA TGGTCTGATT CATAACTCAG GCGACTGCCG GAGTAGCACC TGTGAAGGCC TCGACCTACT CAGGAAAATT TCAAATGCAC AACGGATGGG CTGTGTCCTC ATAGGGCCCT CATGTACATA CTCCACCTTC CAGATGTACC TTGACACAGA ATTGAGCTAC CCCATGATCT CAGCTGGAAG TTTTGGATTG TCATGTGACT ATAAAGAAAC CTTAACCAGG CTGATGTCTC CAGCTAGAAA GTTGATGTAC TTCTTGGTTA ACTTTTGGAA AACCAACGAT CTGCCCTTCA AAACTTATTC CTGGAGCACT TCGTATGTTT ACAAGAATGG TACAGAAACT GAGGACTGTT TCTGGTACCT TAATGCTCTG GAGGCTAGCG TTTCCTATTT CTCCCACGAA CTCGGCTTTA AGGTGGTGTT AAGACAAGAT AAGGAGTTTC AGGATATCTT AATGGACCAC AACAGGAAAA GCAATGTGAT TATTATGTGT GGTGGTCCAG AGTTCCTCTA CAAGCTGAAG GGTGACCGAG CAGTGGCTGA AGACATTGTC ATTATTCTAG TGGATCTTTT CAATGACCAG TACTTTGAGG ACAATGTCAC AGCCCCTGAC TATATGAAAA ATGTCCTTGT TCTGACGCTG TCTCCTGGGA ATTCCCTTCT AAATAGCTCT TTCTCCAGGA ATCTATCACC AACAAAACGA GACTTTGCTC TTGCCTATTT GAATGGAATC CTGCTCTTTG GACATATGCT GAAGATATTT CTTGAAAATG GAGAAAATAT TACCACCCCC AAATTTGCTC ATGCTTTCAG GAATCTCACT TTTGAAGGGT ATGACGGTCC AGTGACCTTG GATGACTGGG GGGATGTTGA CAGTACCATG GTGCTTCTGT ATACCTCTGT GGACACCAAG AAATACAAGG TTCTTTTGAC CTATGATACC CACGTAAATA AGACCTATCC TGTGGATATG AGCCCCACAT TCACTTGGAA GAACTCTAAA CTTCCTAATG ATATTACAGG CCGGGGCCCT CAGATCCTGA TGATTGCAGT CTTCACCCTC ACTGGAGCTG TGGTGCTGCT CCTGCTCGTC GCTCTCCTGA TGCTCAGAAA ATATAGAAAA GATTATGAAC TTCGTCAGAA AAAATGGTCC CACATTCCTC CTGAAAATAT CTTTCCTCTG GAGACCAATG AGACCAATCA TGTTAGCCTC AAGATCGATG ATGACAAAAG ACGAGATACA ATCCAGAGAC TACGACAGTG CAAATACGAC AAAAAGCGAG TGATTCTCAA AGATCTCAAG CACAATGATG GTAATTTCAC TGAAAAACAG AAGATAGAAT TGAACAAGTT GCTTCAGATT GACTATTACA ACCTGACCAA GTTCTACGGC ACAGTGAAAC TTGATACCAT GATCTTCGGG GTGATAGAAT ACTGTGAGAG AGGATCCCTC CGGGAAGTTT TAAATGACAC AATTTCCTAC CCTGATGGCA CATTCATGGA TTGGGAGTTT AAGATCTCTG TCTTGTATGA CATTGCTAAG GGAATGTCAT ATCTGCACTC CAGTAAGACA GAAGTCCATG GTCGTCTGAA ATCTACCAAC TGCGTAGTGG ACAGTAGAAT GGTGGTGAAG ATCACTGATT TTGGCTGCAA TTCCATTTTA CCTCCAAAAA AGGACCTGTG GACAGCTCCA GAGCACCTCC GCCAAGCCAA CATCTCTCAG AAAGGAGATG TGTACAGCTA TGGGATCATC GCACAGGAGA TCATCCTGCG GAAAGAAACC TTCTACACTT TGAGCTGTCG GGACCGGAAT GAGAAGATTT TCAGAGTGGA AAATTCCAAT GGAATGAAAC CCTTCCGCCC AGATTTATTC TTGGAAACAG CAGAGGAAAA AGAGCTAGAA GTGTACCTAC TTGTAAAAAA CTGTTGGGAG GAAGATCCAG AAAAGAGACC AGATTTCAAA AAAATTGAGA CTACACTTGC CAAGATATTT GGACTTTTTC ATGACCAAAA AAATGAAAGC TATATGGATA CCTTGATCCG ACGTCTACAG CTATATTCTC GAAACCTGGA ACATCTGGTA GAGGAAAGGA CACAGCTGTA CAAGGCAGAG AGGGACAGGG CTGACAGACT TAACTTTATG TTGCTTCCAA GGCTAGTGGT AAAGTCTCTG AAGGAGAAAG GCTTTGTGGA GCCGGAACTA TATGAGGAAG TTACAATCTA CTTCAGTGAC ATTGTAGGTT TCACTACTAT CTGCAAATAC AGCACCCCCA TGGAAGTGGT GGACATGCTT AATGACATCT ATAAGAGTTT TGACCACATT GTTGATCATC ATGATGTCTA CAAGGTGGAA ACCATCGGTG ATGCGTACAT GGTGGCTAGT GGTTTGCCTA AGAGAAATGG CAATCGGCAT GCAATAGACA TTGCCAAGAT GGCCTTGGAA ATCCTCAGCT TCATGGGGAC CTTTGAGCTG GAGCATCTTC CTGGCCTCCC AATATGGATT CGCATTGGAG TTCACTCTGG TCCCTGTGCT GCTGGAGTTG TGGGAATCAA GATGCCTCGT TATTGTCTAT TTGGAGATAC GGTCAACACA GCCTCTAGGA TGGAATCCAC TGGCCTCCCT TTGAGAATTC ACGTGAGTGG CTCCACCATA GCCATCCTGA AGAGAACTGA GTGCCAGTTC CTTTATGAAG TGAGAGGAGA AACATACTTA AAGGGAAGAG GAAATGAGAC TACCTACTGG CTGACTGGGA TGAAGGACCA GAAATTCAAC CTGCCAACCC CTCCTACTGT GGAGAATCAA CAGCGTTTGC AAGCAGAATT TTCAGACATG ATTGCCAACT CTTTACAGAA AAGACAGGCA GCAGGGATAA GAAGCCAAAA ACCCAGACGG GTAGCCAGCT ATAAAAAAGG CACTCTGGAA TACTTGCAGC TGAATACCAC AGACAAGGAG AGCACCTATT TTTAAGCGGC CGCGAAGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC CGGCGCCTAC GCTAGACGCC ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCAGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGAATTGACT CAAATGATGT CAATTAGTCT ATCAGAAGCT ATCTGGTCTC CCTTCCGGGG GACAAGACAT CCCTGTTTAA TATTTAAACA GCAGTGTTCC CAAACTGGGT TCTTATATCC CTTGCTCTGG TCAACCAGGT TGCAGGGTTT CCTGTCCTCA CAGGAACGAA GTCCCTAAAG AAACAGTGGC AGCCAGGTTT AGCCCCGGAA TTGACTGGAT TCCTTTTTTA GGGCCCATTG GTATGGCTTT TTCCCCGTAT CCCCCCAGGT GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG TTCAGAGGAA AGCGATCCCG TGCCACCTTC CCCGTGCCCG GGCTGTCCCC GCACGCTGCC GGCTCGGGGA TGCGGGGGGA GCGCCGGACC GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC CCCCTAGCGG GGGAGGGACG TAATTACATC CCTGGGGGCT TTGGGGGGGG GCTGTCCCTG ATATCTATAA CAAGAAAATA TATATATAAT AAGTTATCAC GTAAGTAGAA CATGAAATAA CAATATAATT ATCGTATGAG TTAAATCTTA AAAGTCACGT AAAAGATAAT CATGCGTCAT TTTGACTCAC GCGGTCGTTA TAGTTCAAAA TCAGTGACAC TTACCGCATT GACAAGCACG CCTCACGGGA GCTCCAAGCG GCGACTGAGA TGTCCTAAAT GCACAGCGAC GGATTCGCGC TATTTAGAAA GAGAGAGCAA TATTTCAAGA ATGCATGCGT CAATTTTACG CAGACTATCT TTCTAGGGTT AATCTAGCTG CATCAGGATC ATATCGTCGG GTCTTTTTTC CGGCTCAGTC ATCGCCCAAG CTGGCGCTAT CTGGGCATCG GGGAGGAAGA AGCCCGTGCC TTTTCCCGCG AGGTTGAAGC GGCATGGAAA GAGTTTGCCG AGGATGACTG CTGCTGCATT GACGTTGAGC GAAAACGCAC GTTTACCATG ATGATTCGGG AAGGTGTGGC CATGCACGCC TTTAACGGTG AACTGTTCGT TCAGGCCACC TGGGATACCA GTTCGTCGCG GCTTTTCCGG ACACAGTTCC GGATGGTCAG CCCGAAGCGC ATCAGCAACC CGAACAATAC CGGCGACAGC CGGAACTGCC GTGCCGGTGT GCAGATTAAT GACAGCGGTG CGGCGCTGGG ATATTACGTC AGCGAGGACG GGTATCCTGG CTGGATGCCG CAGAAATGGA CATGGATACC CCGTGAGTTA CCCGGCGGGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
