pTX172-AG-sgRNA1-UBI-Cas9
$84.00
In stock
SKU
PL55774
100μg liquid plasmid(Instantly):$105
| name | pTX172-AG-sgRNA1-UBI-Cas9 | another name | apTX172-AG-sgRNA1-UBI-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | UBI | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGATATTCTT GGAGTAGACG AGAGTGTCGT GCTCCACCAT GGGCGCGCCT GCAGTGCAGC GTGACCCGGT CGTGCCCCTC TCTTGAGATA AGGTGAGCAT TGCATGTCTA AGTTATAAAA AATTACCACA TATTTTTTTT GTCACACTTG TTTGAAGTGC AGTTTATCTA TCTTTATACA TATATTTAAA CTTTACTCTA CGAATAATAT AATCTATAGT ACTACAATAA TATCAGTGTT TTAGAGAATC ATATAAATGA ACAGTTAGAC ATGGTCTAAA GGACAATTGA GTATTTTGAC AACAGGACTC TACAGTTTTA TCTTTTTAGT GTGCATGTGT TCTCCTTTTT TTTTGCAAAT AGCTTCACCT ATATAATACT TCATCCATTT TATTAGTACA TCCATTTAGG GTTTAGGGTT AATGGTTTTT ATAGACTAAT TTTTTTAGTA CATCTATTTT ATTCTATTTT AGCCTCTAAA TTAAGAAAAC TAAAACTCTA TTTTAGTTTT TTTATTTAAT AATTTAGATA TAAAATAGAA TAAAATAAAG TGACTAAAAA TTAAACAAAT ACCCTTTAAG AAATTAAAAA AACTAAGGAA ACATTTTTCT TGTTTCGAGT AGATAATGCC AGCCTGTTAA ACGCCGTCGA CGAGTCTAAC GGACACCAAC CAGCGAACCA GCAGCGTCGC GTCGGGCCAA GCGAAGCAGA CGGCACGGCA TCTCTGTCGC TGCCTCTGGA CCCCTCTCGA GAGTTCCGCT CCACCGTTGG ACTTGCTCCG CTGTCGGCAT CCAGAAATTG CGTGGCGGAG CGGCAGACGT GAGCCGGCAC GGCAGGCGGC CTCCTCCTCC TCTCACGGCA CCGGCAGCTA CGGGGGATTC CTTTCCCACC GCTCCTTCGC TTTCCCTTCC TCGCCCGCCG TAATAAATAG ACACCCCCTC CACACCCTCT TTCCCCAACC TCGTGTTGTT CGGAGCGCAC ACACACACAA CCAGAACTCC CCCAAATCCA CCCGTCGGCA CCTCCGCTTC AAGGTACGCC GCTCGTCCTC CCCCCCCCCC TCTCTACCTT CTCAAGATCG GCGTTCCGGT CCATGGTTAG GGCCCGGTAG TTCTACTTCT GTTCATGTTT GTGTTAGATC CGTGTTTGTG TTAGATCCGT GCTACTAGCG TTCGTACACG GATGCGACCT GTACGTCAGA CACGTTCTGA TTGCTAACTT GCCAGTGTTT CTCTTTGGGG AATCCTGGGA TGGCTCTAGC CGTTCCGCAG ACGGGATCGA TTTCATGATT TTTTTTGTTT CGTTGCATAG GGTTTGGTTT GCCCTTTTCC TTTATTTCAA TATATGCCGT GCACTTGTTT GTCGGGTCAT CTTTTCATGC TTTTTTTTGT CTTGGTTGTG ATGATGTGGT CTGGTTGGGC GGTCGTTCAA GATCGGAGTA GAATTAATTC TGTTTCAAAC TACCTGGTGG ATTTATTAAT TTTGGATCTG TATGTGTGTG CCATACATAT TCATAGTTAC GAATTGAAGA TGATGGATGG AAATATCGAT CTAGGATAGG TATACATGTT GATGCGGGTT TTACTGATGC ATATACAGAG ATGCTTTTTG TTCGCTTGGT TGTGATGATG TGGTGTGGTT GGGCGGTCGT TCATTCGTTC AAGATCGGAG TAGAATACTG TTTCAAACTA CCTGGTGTAT TTATTAATTT TGGAACTGTA TGTGTGTGTC ATACATCTTC ATAGTTACGA GTTTAAGATG GATGGAAATA TCGATCTAGG ATAGGTATAC ATGTTGATGT GGGTTTTACT GATGCATATA CATGATGGCA TATGCAGCAT CTATTCATAT GCTCTAACCT TGAGTACCTA TCTATTATAA TAAACAAGTA TGTTTTATAA TTATTTTGAT CTTGATATAC TTGGATGATG GCATATGCAG CAGCTATATG TGGATTTTTT TAGCCCTGCC TTCATACGCT ATTTATTTGC TTGGTACTGT TTCTTTTGTC GATGCTCACC CTGTTGTTTG GTGTTACTTC TGCAGCCTGC AGGTAGATCG CTCGTCGACA TGGATAAGAA GTACTCTATC GGACTCGATA TCGGAACTAA CTCTGTGGGA TGGGCTGTGA TCACCGATGA GTACAAGGTG CCATCTAAGA AGTTCAAGGT TCTCGGAAAC ACCGATAGGC ACTCTATCAA GAAAAACCTT ATCGGTGCTC TCCTCTTCGA TTCTGGTGAA ACTGCTGAGG CTACCAGACT CAAGAGAACC GCTAGAAGAA GGTACACCAG AAGAAAGAAC AGGATCTGCT ACCTCCAAGA GATCTTCTCT AACGAGATGG CTAAAGTGGA TGATTCATTC TTCCACAGGC TCGAAGAGTC ATTCCTCGTG GAAGAAGATA AGAAGCACGA GAGGCACCCT ATCTTCGGAA ACATCGTTGA TGAGGTGGCA TACCACGAGA AGTACCCTAC TATCTACCAC CTCAGAAAGA AGCTCGTTGA TTCTACTGAT AAGGCTGATC TCAGGCTCAT CTACCTCGCT CTCGCTCACA TGATCAAGTT CAGAGGACAC TTCCTCATCG AGGGTGATCT CAACCCTGAT AACTCTGATG TGGATAAGTT GTTCATCCAG CTCGTGCAGA CCTACAACCA GCTTTTCGAA GAGAACCCTA TCAACGCTTC AGGTGTGGAT GCTAAGGCTA TCCTCTCTGC TAGGCTCTCT AAGTCAAGAA GGCTTGAGAA CCTCATTGCT CAGCTCCCTG GTGAGAAGAA GAACGGACTT TTCGGAAACT TGATCGCTCT CTCTCTCGGA CTCACCCCTA ACTTCAAGTC TAACTTCGAT CTCGCTGAGG ATGCAAAGCT CCAGCTCTCA AAGGATACCT ACGATGATGA TCTCGATAAC CTCCTCGCTC AGATCGGAGA TCAGTACGCT GATTTGTTCC TCGCTGCTAA GAACCTCTCT GATGCTATCC TCCTCAGTGA TATCCTCAGA GTGAACACCG AGATCACCAA GGCTCCACTC TCAGCTTCTA TGATCAAGAG ATACGATGAG CACCACCAGG ATCTCACACT TCTCAAGGCT CTTGTTAGAC AGCAGCTCCC AGAGAAGTAC AAAGAGATTT TCTTCGATCA GTCTAAGAAC GGATACGCTG GTTACATCGA TGGTGGTGCA TCTCAAGAAG AGTTCTACAA GTTCATCAAG CCTATCCTCG AGAAGATGGA TGGAACCGAG GAACTCCTCG TGAAGCTCAA TAGAGAGGAT CTTCTCAGAA AGCAGAGGAC CTTCGATAAC GGATCTATCC CTCATCAGAT CCACCTCGGA GAGTTGCACG CTATCCTTAG AAGGCAAGAG GATTTCTACC CATTCCTCAA GGATAACAGG GAAAAGATTG AGAAGATTCT CACCTTCAGA ATCCCTTACT ACGTGGGACC TCTCGCTAGA GGAAACTCAA GATTCGCTTG GATGACCAGA AAGTCTGAGG AAACCATCAC CCCTTGGAAC TTCGAAGAGG TGGTGGATAA GGGTGCTAGT GCTCAGTCTT TCATCGAGAG GATGACCAAC TTCGATAAGA ACCTTCCAAA CGAGAAGGTG CTCCCTAAGC ACTCTTTGCT CTACGAGTAC TTCACCGTGT ACAACGAGTT GACCAAGGTT AAGTACGTGA CCGAGGGAAT GAGGAAGCCT GCTTTTTTGT CAGGTGAGCA AAAGAAGGCT ATCGTTGATC TCTTGTTCAA GACCAACAGA AAGGTGACCG TGAAGCAGCT CAAAGAGGAT TACTTCAAGA AAATCGAGTG CTTCGATTCA GTTGAGATTT CTGGTGTTGA GGATAGGTTC AACGCATCTC TCGGAACCTA CCACGATCTC CTCAAGATCA TTAAGGATAA GGATTTCTTG GATAACGAGG AAAACGAGGA TATCTTGGAG GATATCGTTC TTACCCTCAC CCTCTTTGAA GATAGAGAGA TGATTGAAGA AAGGCTCAAG ACCTACGCTC ATCTCTTCGA TGATAAGGTG ATGAAGCAGT TGAAGAGAAG AAGATACACT GGTTGGGGAA GGCTCTCAAG AAAGCTCATT AACGGAATCA GGGATAAGCA GTCTGGAAAG ACAATCCTTG ATTTCCTCAA GTCTGATGGA TTCGCTAACA GAAACTTCAT GCAGCTCATC CACGATGATT CTCTCACCTT TAAAGAGGAT ATCCAGAAGG CTCAGGTTTC AGGACAGGGT GATAGTCTCC ATGAGCATAT CGCTAACCTC GCTGGATCTC CTGCAATCAA GAAGGGAATC CTCCAGACTG TGAAGGTTGT GGATGAGTTG GTGAAGGTGA TGGGAAGGCA TAAGCCTGAG AACATCGTGA TCGAAATGGC TAGAGAGAAC CAGACCACTC AGAAGGGACA GAAGAACTCT AGGGAAAGGA TGAAGAGGAT CGAGGAAGGT ATCAAAGAGC TTGGATCTCA GATCCTCAAA GAGCACCCTG TTGAGAACAC TCAGCTCCAG AATGAGAAGC TCTACCTCTA CTACCTCCAG AACGGAAGGG ATATGTATGT GGATCAAGAG TTGGATATCA ACAGGCTCTC TGATTACGAT GTTGATCATA TCGTGCCACA GTCATTCTTG AAGGATGATT CTATCGATAA CAAGGTGCTC ACCAGGTCTG ATAAGAACAG GGGTAAGAGT GATAACGTGC CAAGTGAAGA GGTTGTGAAG AAAATGAAGA ACTATTGGAG GCAGCTCCTC AACGCTAAGC TCATCACTCA GAGAAAGTTC GATAACTTGA CTAAGGCTGA GAGGGGAGGA CTCTCTGAAT TGGATAAGGC AGGATTCATC AAGAGGCAGC TTGTGGAAAC CAGGCAGATC ACTAAGCACG TTGCACAGAT CCTCGATTCT AGGATGAACA CCAAGTACGA TGAGAACGAT AAGTTGATCA GGGAAGTGAA GGTTATCACC CTCAAGTCAA AGCTCGTGTC TGATTTCAGA AAGGATTTCC AATTCTACAA GGTGAGGGAA ATCAACAACT ACCACCACGC TCACGATGCT TACCTTAACG CTGTTGTTGG AACCGCTCTC ATCAAGAAGT ATCCTAAGCT CGAGTCAGAG TTCGTGTACG GTGATTACAA GGTGTACGAT GTGAGGAAGA TGATCGCTAA GTCTGAGCAA GAGATCGGAA AGGCTACCGC TAAGTATTTC TTCTACTCTA ACATCATGAA TTTCTTCAAG ACCGAGATTA CCCTCGCTAA CGGTGAGATC AGAAAGAGGC CACTCATCGA GACAAACGGT GAAACAGGTG AGATCGTGTG GGATAAGGGA AGGGATTTCG CTACCGTTAG AAAGGTGCTC TCTATGCCAC AGGTGAACAT CGTTAAGAAA ACCGAGGTGC AGACCGGTGG ATTCTCTAAA GAGTCTATCC TCCCTAAGAG GAACTCTGAT AAGCTCATTG CTAGGAAGAA GGATTGGGAC CCTAAGAAAT ACGGTGGTTT CGATTCTCCT ACCGTGGCTT ACTCTGTTCT CGTTGTGGCT AAGGTTGAGA AGGGAAAGAG TAAGAAGCTC AAGTCTGTTA AGGAACTTCT CGGAATCACT ATCATGGAAA GGTCATCTTT CGAGAAGAAC CCAATCGATT TCCTCGAGGC TAAGGGATAC AAAGAGGTTA AGAAGGATCT CATCATCAAG CTCCCAAAGT ACTCACTCTT CGAACTCGAG AACGGTAGAA AGAGGATGCT CGCTTCTGCT GGTGAGCTTC AAAAGGGAAA CGAGCTTGCT CTCCCATCTA AGTACGTTAA CTTTCTTTAC CTCGCTTCTC ACTACGAGAA GTTGAAGGGA TCTCCAGAAG ATAACGAGCA GAAGCAACTT TTCGTTGAGC AGCACAAGCA CTACTTGGAT GAGATCATCG AGCAGATCTC TGAGTTCTCT AAAAGGGTGA TCCTCGCTGA TGCAAACCTC GATAAGGTGT TGTCTGCTTA CAACAAGCAC AGAGATAAGC CTATCAGGGA ACAGGCAGAG AACATCATCC ATCTCTTCAC CCTTACCAAC CTCGGTGCTC CTGCTGCTTT CAAGTACTTC GATACAACCA TCGATAGGAA GAGATACACC TCTACCAAAG AAGTGCTCGA TGCTACCCTC ATCCATCAGT CTATCACTGG ACTCTACGAG ACTAGGATCG ATCTCTCACA GCTCGGTGGT GATTCAAGGG CTGATCCTAA GAAGAAGAGG AAGGTTTGAG GATCTAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAGGCTT TTGTCACCGG AGGACAATTC TAACACCGGA TGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TGTCACCGGA TGTGTTTTCC GGTCTGATGA GTCCGTGAGG ACGAAACAAA AGCGAGCTCA AACTATCAGT GTTTGACAGG ATATATTGGC GGGTAAACCT AAGAGAAAAG AGCGTTTATT AGAATAATCG GATATTTAAA AGGGCGTGAA AAGGTTTATC CGTTCGTCCA TTTGTATGTG CATGCCAACC ACAGGGTTCC CCTCGGGATC AAAGTACTTT GATCCAACCC CTCCGCTGCT ATAGTGCAGT CGGCTTCTGA CGTTCAGTGC AGCCGTCTTC TGAAAACGAC ATGTCGCACA AGTCCTAAGT TACGCGACAG GCTGCCGCCC TGCCCTTTTC CTGGCGTTTT CTTGTCGCGT GTTTTAGTCG CATAAAGTAG AATACTTGCG ACTAGAACCG GAGACATTAC GCCATGAACA AGAGCGCCGC CGCTGGCCTG CTGGGCTATG CCCGCGTCAG CACCGACGAC CAGGACTTGA CCAACCAACG GGCCGAACTG CACGCGGCCG GCTGCACCAA GCTGTTTTCC GAGAAGATCA CCGGCACCAG GCGCGACCGC CCGGAGCTGG CCAGGATGCT TGACCACCTA CGCCCTGGCG ACGTTGTGAC AGTGACCAGG CTAGACCGCC TGGCCCGCAG CACCCGCGAC CTACTGGACA TTGCCGAGCG CATCCAGGAG GCCGGCGCGG GCCTGCGTAG CCTGGCAGAG CCGTGGGCCG ACACCACCAC GCCGGCCGGC CGCATGGTGT TGACCGTGTT CGCCGGCATT GCCGAGTTCG AGCGTTCCCT AATCATCGAC CGCACCCGGA GCGGGCGCGA GGCCGCCAAG GCCCGAGGCG TGAAGTTTGG CCCCCGCCCT ACCCTCACCC CGGCACAGAT CGCGCACGCC CGCGAGCTGA TCGACCAGGA AGGCCGCACC GTGAAAGAGG CGGCTGCACT GCTTGGCGTG CATCGCTCGA CCCTGTACCG CGCACTTGAG CGCAGCGAGG AAGTGACGCC CACCGAGGCC AGGCGGCGCG GTGCCTTCCG TGAGGACGCA TTGACCGAGG CCGACGCCCT GGCGGCCGCC GAGAATGAAC GCCAAGAGGA ACAAGCATGA AACCGCACCA GGACGGCCAG GACGAACCGT TTTTCATTAC CGAAGAGATC GAGGCGGAGA TGATCGCGGC CGGGTACGTG TTCGAGCCGC CCGCGCACGG CTCAACCGTG CGGCTGCATG AAATCCTGGC CGGTTTGTCT GATGCCAAGC TGGCGGCCTG GCCGGCCAGC TTGGCCGCTG AAGAAACCGA GCGCCGCCGT CTAAAAAGGT GATGTGTATT TGAGTAAAAC AGCTTGCGTC ATGCGGTCGC TGCGTATATG ATGCGATGAG TAAATAAACA AATACGCAAG GGGAACGCAT GAAGGTTATC GCTGTACTTA ACCAGAAAGG CGGGTCAGGC AAGACGACCA TCGCAACCCA TCTAGCCCGC GCCCTGCAAC TCGCCGGGGC CGATGTTCTG TTAGTCGATT CCGATCCCCA GGGCAGTGCC CGCGATTGGG CGGCCGTGCG GGAAGATCAA CCGCTAACCG TTGTCGGCAT CGACCGCCCG ACGATTGACC GCGACGTGAA GGCCATCGGC CGGCGCGACT TCGTAGTGAT CGACGGAGCG CCCCAGGCGG CGGACTTGGC TGTGTCCGCG ATCAAGGCAG CCGACTTCGT GCTGATTCCG GTGCAGCCAA GCCCTTACGA CATATGGGCC ACCGCCGACC TGGTGGAGCT GGTTAAGCAG CGCATTGAGG TCACGGATGG AAGGCTACAA GCGGCCTTTG TCGTGTCGCG GGCGATCAAA GGCACGCGCA TCGGCGGTGA GGTTGCCGAG GCGCTGGCCG GGTACGAGCT GCCCATTCTT GAGTCCCGTA TCACGCAGCG CGTGAGCTAC CCAGGCACTG CCGCCGCCGG CACAACCGTT CTTGAATCAG AACCCGAGGG CGACGCTGCC CGCGAGGTCC AGGCGCTGGC CGCTGAAATT AAATCAAAAC TCATTTGAGT TAATGAGGTA AAGAGAAAAT GAGCAAAAGC ACAAACACGC TAAGTGCCGG CCGTCCGAGC GCACGCAGCA GCAAGGCTGC AACGTTGGCC AGCCTGGCAG ACACGCCAGC CATGAAGCGG GTCAACTTTC AGTTGCCGGC GGAGGATCAC ACCAAGCTGA AGATGTACGC GGTACGCCAA GGCAAGACCA TTACCGAGCT GCTATCTGAA TACATCGCGC AGCTACCAGA GTAAATGAGC AAATGAATAA ATGAGTAGAT GAATTTTAGC GGCTAAAGGA GGCGGCATGG AAAATCAAGA ACAACCAGGC ACCGACGCCG TGGAATGCCC CATGTGTGGA GGAACGGGCG GTTGGCCAGG CGTAAGCGGC TGGGTTGCCT GCCGGCCCTG CAATGGCACT GGAACCCCCA AGCCCGAGGA ATCGGCGTGA GCGGTCGCAA ACCATCCGGC CCGGTACAAA TCGGCGCGGC GCTGGGTGAT GACCTGGTGG AGAAGTTGAA GGCCGCGCAG GCCGCCCAGC GGCAACGCAT CGAGGCAGAA GCACGCCCCG GTGAATCGTG GCAAGCGGCC GCTGATCGAA TCCGCAAAGA ATCCCGGCAA CCGCCGGCAG CCGGTGCGCC GTCGATTAGG AAGCCGCCCA AGGGCGACGA GCAACCAGAT TTTTTCGTTC CGATGCTCTA TGACGTGGGC ACCCGCGATA GTCGCAGCAT CATGGACGTG GCCGTTTTCC GTCTGTCGAA GCGTGACCGA CGAGCTGGCG AGGTGATCCG CTACGAGCTT CCAGACGGGC ACGTAGAGGT TTCCGCAGGG CCGGCCGGCA TGGCCAGTGT GTGGGATTAC GACCTGGTAC TGATGGCGGT TTCCCATCTA ACCGAATCCA TGAACCGATA CCGGGAAGGG AAGGGAGACA AGCCCGGCCG CGTGTTCCGT CCACACGTTG CGGACGTACT CAAGTTCTGC CGGCGAGCCG ATGGCGGAAA GCAGAAAGAC GACCTGGTAG AAACCTGCAT TCGGTTAAAC ACCACGCACG TTGCCATGCA GCGTACGAAG AAGGCCAAGA ACGGCCGCCT GGTGACGGTA TCCGAGGGTG AAGCCTTGAT TAGCCGCTAC AAGATCGTAA AGAGCGAAAC CGGGCGGCCG GAGTACATCG AGATCGAGCT AGCTGATTGG ATGTACCGCG AGATCACAGA AGGCAAGAAC CCGGACGTGC TGACGGTTCA CCCCGATTAC TTTTTGATCG ATCCCGGCAT CGGCCGTTTT CTCTACCGCC TGGCACGCCG CGCCGCAGGC AAGGCAGAAG CCAGATGGTT GTTCAAGACG ATCTACGAAC GCAGTGGCAG CGCCGGAGAG TTCAAGAAGT TCTGTTTCAC CGTGCGCAAG CTGATCGGGT CAAATGACCT GCCGGAGTAC GATTTGAAGG AGGAGGCGGG GCAGGCTGGC CCGATCCTAG TCATGCGCTA CCGCAACCTG ATCGAGGGCG AAGCATCCGC CGGTTCCTAA TGTACGGAGC AGATGCTAGG GCAAATTGCC CTAGCAGGGG AAAAAGGTCG AAAAGGCCTC TTTCCTGTGG ATAGCACGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGAACCCG TACATTGGGA ACCCAAAGCC GTACATTGGG AACCGGTCAC ACATGTAAGT GACTGATATA AAAGAGAAAA AAGGCGATTT TTCCGCCTAA AACTCTTTAA AACTTATTAA AACTCTTAAA ACCCGCCTGG CCTGTGCATA ACTGTCTGGC CAGCGCACAG CCGAAGAGCT GCAAAAAGCG CCTACCCTTC GGTCGCTGCG CTCCCTACGC CCCGCCGCTT CGCGTCGGCC TATCGCGGCC GCTGGCCGCT CAAAAATGGC TGGCCTACGG CCAGGCAATC TACCAGGGCG CGGACAAGCC GCGCCGTCGC CACTCGACCG CCGGCGCCCA CATCAAGGCA CCCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAA ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGCATTCTA GGTACTAAAA CAATTCATCC AGTAAAATAT AATATTTTAT TTTCTCCCAA TCAGGCTTGA TCCCCAGTAA GTCAAAAAAT AGCTCGACAT ACTGTTCTTC CCCGATATCC TCCCTGATCG ACCGGACGCA GAAGGCAATG TCATACCACT TGTCCGCCCT GCCGCTTCTC CCAAGATCAA TAAAGCCACT TACTTTGCCA TCTTTCACAA AGATGTTGCT GTCTCCCAGG TCGCCGTGGG AAAAGACAAG TTCCTCTTCG GGCTTTTCCG TCTTTAAAAA ATCATACAGC TCGCGCGGAT CTTTAAATGG AGTGTCTTCT TCCCAGTTTT CGCAATCCAC ATCGGCCAGA TCGTTATTCA GTAAGTAATC CAATTCGGCT AAGCGGCTGT CTAAGCTATT CGTATAGGGA CAATCCGATA TGTCGATGGA GTGAAAGAGC CTGATGCACT CCGCATACAG CTCGATAATC TTTTCAGGGC TTTGTTCATC TTCATACTCT TCCGAGCAAA GGACGCCATC GGCCTCACTC ATGAGCAGAT TGCTCCAGCC ATCATGCCGT TCAAAGTGCA GGACCTTTGG AACAGGCAGC TTTCCTTCCA GCCATAGCAT CATGTCCTTT TCCCGTTCCA CATCATAGGT GGTCCCTTTA TACCGGCTGT CCGTCATTTT TAAATATAGG TTTTCATTTT CTCCCACCAG CTTATATACC TTAGCAGGAG ACATTCCTTC CGTATCTTTT ACGCAGCGGT ATTTTTCGAT CAGTTTTTTC AATTCCGGTG ATATTCTCAT TTTAGCCATT TATTATTTCC TTCCTCTTTT CTACAGTATT TAAAGATACC CCAAGAAGCT AATTATAACA AGACGAACTC CAATTCACTG TTCCTTGCAT TCTAAAACCT TAAATACCAG AAAACAGCTT TTTCAAAGTT GTTTTCAAAG TTGGCGTATA ACATAGTATC GACGGAGCCG ATTTTGAAAC CGCGGTGATC ACAGGCAGCA ACGCTCTGTC ATCGTTACAA TCAACATGCT ACCCTCCGCG AGATCATCCG TGTTTCAAAC CCGGCAGCTT AGTTGCCGTT CTTCCGAATA GCATCGGTAA CATGAGCAAA GTCTGCCGCC TTACAACGGC TCTCCCGCTG ACGCCGTCCC GGACTGATGG GCTGCCTGTA TCGAGTGGTG ATTTTGTGCC GAGCTGCCGG TCGGGGAGCT GTTGGCTGGC TGGTGGCAGG ATATATTGTG GTGTAAACAA ATTGACGCTT AGACAACTTA ATAACACATT GCGGACGTTT TTAATGTACT GAATTAACGC CGAATTAATT CGGGGGATCT GGATTTTAGT ACTGGATTTT GGTTTTAGGA ATTAGAAATT TTATTGATAG AAGTATTTTA CAAATACAAA TACATACTAA GGGTTTCTTA TATGCTCAAC ACATGAGCGA AACCCTATAG GAACCCTAAT TCCCTTATCT GGGAACTACT CACACATTAT TATGGAGAAA ATAGAGCTTG TCGATCGTTA ATTAATCACC TCCAAGATCC CGGACGAGTG CTGGGGCGTC GGTTTCCACT ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG GAGCATATAC GCCCGGAGCC GCGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCACG AGGTGCCGGA CTTCGGGGCA GTCCTCGGCC CAAAGCATCA GCTCATCGAG AGCCTGCGCG ACGGACGCAC TGACGGTGTC GTCCATCACA GTTTGCCAGT GATACACATG GGGATCAGCA ATCGCGCATA TGAAATCACG CCATGTAGTG TATTGACCGA TTCCTTGCGG TCCGAATGGG CCGAACCCGC TCGTCTGGCT AAGATCGGCC GCAGCGATCG CATCCATGGC CTCCGCGACC GGCTGCAGAA CAGCGGGCAG TTCGGTTTCA GGCAGGTCTT GCAACGTGAC ACCCTGTGCA CGGCGGGAGA TGCAATAGGT CAGGCTCTCG CTGAATTCCC CAATGTCAAG CACTTCCGGA ATCGGGAGCG CGGCCGATGC AAAGTGCCGA TAAACATAAC GATCTTTGTA GAAACCATCG GCGCAGCTAT TTACCCGCAG GACATATCCA CGCCCTCCTA CATCGAAGCT GAAAGCACGA GATTCTTCGC CCTCCGAGAG CTGCATCAGG TCGGACACGC TGTCGAACTT TTCGATCAGA AACTTCTCGA CAGACGTCGC GGTGAGTTCA GGCTTTTTCA TCCTAGGAGA GATAGATTTG TAGAGAGAGA CTGGTGATTT CAGCGTGTCC TCTCCAAATG AAATGAACTT CCTTATATAG AGGAAGGGTC TTGCGAAGGA TAGTGGGATT GTGCGTCATC CCTTACGTCA GTGGAGATAT CACATCAATC CACTTGCTTT GAAGACGTGG TTGGAACGTC TTCTTTTTCC ACGATGCTCC TCGTGGGTGG GGGTCCATCT TTGGGACCAC TGTCGGCAGA GGCATCTTGA ACGATAGCCT TTCCTTTATC GCAATGATGG CATTTGTAGG TGCCACCTTC CTTTTCTACT GTCCTTTTGA TGAAGTGACA GATAGCTGGG CAATGGAATC CGAGGAGGTT TCCCGATATT ACCCTTTGTT GAAAAGTCTC AATAGCCCTT TGGTCTTCTG AGACTGTATC TTTGATATTC TTGGAGTAGA CGAGAGTGTC GTGCTCCACC ATGTTCACAT CAATCCACTT GCTTTGAAGA CGTGGTTGGA ACGTCTTCTT TTTCCACGAT GCTCCTCGTG GGTGGGGGTC CATCTTTGGG ACCACTGTCG GCAGAGGCAT CTTGAACGAT AGCCTTTCCT TTATCGCAAT GATGGCATTT GTAGGTGCCA CCTTCCTTTT CTACTGTCCT TTTGATGAAG TGACAGATAG CTGGGCAATG GAATCCGAGG AGGTTTCCCG ATATTACCCT TTGTTGAAAA GTCTCAATAG CCCTTTGGTC TTCTGAGACT GTATCTT
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