pBUE411-AtU6-FPA-BRI1-sgRNA1-Ubi-Cas9
$84.00
In stock
SKU
PL55632
100μg liquid plasmid(Instantly):$105
| name | pBUE411-AtU6-FPA-BRI1-sgRNA1-Ubi-Cas9 | another name | apBUE411-AtU6-FPA-BRI1-sgRNA1-Ubi-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Basta | Fluorescent labeling | |
| Promoter | AtU6 | Replicon | pVS1 |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TGCTCTAGCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTCGACTTG CCTTCCGCAC AATACATCAT TTCTTCTTAG CTTTTTTTCT TCTTCTTCGT TCATACAGTT TTTTTTTGTT TATCAGCTTA CATTTTCTTG AACCGTAGCT TTCGTTTTCT TCTTTTTAAC TTTCCATTCG GAGTTTTTGT ATCTTGTTTC ATAGTTTGTC CCAGGATTAG AATGATTAGG CATCGAACCT TCAAGAATTT GATTGAATAA AACATCTTCA TTCTTAAGAT ATGAAGATAA TCTTCAAAAG GCCCCTGGGA ATCTGAAAGA AGAGAAGCAG GCCCATTTAT ATGGGAAAGA ACAATAGTAT TTCTTATATA GGCCCATTTA AGTTGAAAAC AATCTTCAAA AGTCCCACAT CGCTTAGATA AGAAAACGAA GCTGAGTTTA TATACAGCTA GAGTCGAAGT AGTGATTGCT CTTCCTCCAG GTCATCCTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT TTGCAAAATT TTCCAGATCG ATTTCTTCTT CCTCTGTTCT TCGGCCATCG CTACCTTAGG ACCGTTATAG TTAATTACCC TGTTATCCCT ATTAATTAAG AGCTCAGAAA TCTCAAAATT CCGGCAGAAC AATTTTGAAT CTCGATCCGT AGAAACGAGA CGGTCATTGT TTTAGTTCCA CCACGATTAT ATTTGAAATT TACGTGAGTG TGAGTGAGAC TTGCATAAGA AAATAAAATC TTTAGTTGGG AAAAAATTCA ATAATATAAA TGGGCTTGAG AAGGAAGCGA GGGATAGGCC TTTTTCTAAA ATAGGCCCAT TTAAGCTATT AACAATCTTC AAAAGTACCA CAGCGCTTAG GTAAAGAAAG CAGCTGAGTT TATATATGGT TAGAGACGAA GTAGTGATTG TTGGGTCATA ACGATATCTC GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTTCTAGACC CAGCTTTCTT GTACAAGCTT GCATGCCTGC AGTGCAGCGT GACCCGGTCG TGCCCCTCTC TAGAGATAAT GAGCATTGCA TGTCTAAGTT ATAAAAAATT ACCACATATT TTTTTTGTCA CACTTGTTTG AAGTGCAGTT TATCTATCTT TATACATATA TTTAAACTTT ACTCTACGAA TAATATAATC TATAGTACTA CAATAATATC AGTGTTTTAG AGAATCATAT AAATGAACAG TTAGACATGG TCTAAAGGAC AATTGAGTAT TTTGACAACA GGACTCTACA GTTTTATCTT TTTAGTGTGC ATGTGTTCTC CTTTTTTTTT GCAAATAGCT TCACCTATAT AATACTTCAT CCATTTTATT AGTACATCCA TTTAGGGTTT AGGGTTAATG GTTTTTATAG ACTAATTTTT TTAGTACATC TATTTTATTC TATTTTAGCC TCTAAATTAA GAAAACTAAA ACTCTATTTT AGTTTTTTTA TTTAATAATT TAGATATAAA ATAGAATAAA ATAAAGTGAC TAAAAATTAA ACAAATACCC TTTAAGAAAT TAAAAAAACT AAGGAAACAT TTTTCTTGTT TCGAGTAGAT AATGCCAGCC TGTTAAACGC CGTCGACGAG TCTAACGGAC ACCAACCAGC GAACCAGCAG CGTCGCGTCG GGCCAAGCGA AGCAGACGGC ACGGCATCTC TGTCGCTGCC TCTGGACCCC TCTCGAGAGT TCCGCTCCAC CGTTGGACTT GCTCCGCTGT CGGCATCCAG AAATTGCGTG GCGGAGCGGC AGACGTGAGC CGGCACGGCA GGCGGCCTCC TCCTCCTCTC ACGGCACGGC AGCTACGGGG GATTCCTTTC CCACCGCTCC TTCGCTTTCC CTTCCTCGCC CGCCGTAATA AATAGACACC CCCTCCACAC CCTCTTTCCC CAACCTCGTG TTGTTCGGAG CGCACACACA CACAACCAGA TCTCCCCCAA ATCCACCCGT CGGCACCTCC GCTTCAAGGT ACGCCGCTCG TCCTCCCCCC CCCCCCCTCT CTACCTTCTC TAGATCGGCG TTCCGGTCCA TGGTTAGGGC CCGGTAGTTC TACTTCTGTT CATGTTTGTG TTAGATCCGT GTTTGTGTTA GATCCGTGCT GCTAGCGTTC GTACACGGAT GCGACCTGTA CGTCAGACAC GTTCTGATTG CTAACTTGCC AGTGTTTCTC TTTGGGGAAT CCTGGGATGG CTCTAGCCGT TCCGCAGACG GGATCGATTT CATGATTTTT TTTGTTTCGT TGCATAGGGT TTGGTTTGCC CTTTTCCTTT ATTTCAATAT ATGCCGTGCA CTTGTTTGTC GGGTCATCTT TTCATGCTTT TTTTTGTCTT GGTTGTGATG ATGTGGTCTG GTTGGGCGGT CGTTCTAGAT CGGAGTAGAA TTCTGTTTCA AACTACCTGG TGGATTTATT AATTTTGGAT CTGTATGTGT GTGCCATACA TATTCATAGT TACGAATTGA AGATGATGGA TGGAAATATC GATCTAGGAT AGGTATACAT GTTGATGCGG GTTTTACTGA TGCATATACA GAGATGCTTT TTGTTCGCTT GGTTGTGATG ATGTGGTGTG GTTGGGCGGT CGTTCATTCG TTCTAGATCG GAGTAGAATA CTGTTTCAAA CTACCTGGTG TATTTATTAA TTTTGGAACT GTATGTGTGT GTCATACATC TTCATAGTTA CGAGTTTAAG ATGGATGGAA ATATCGATCT AGGATAGGTA TACATGTTGA TGTGGGTTTT ACTGATGCAT ATACATGATG GCATATGCAG CATCTATTCA TATGCTCTAA CCTTGAGTAC CTATCTATTA TAATAAACAA GTATGTTTTA TAATTATTTT GATCTTGATA TACTTGGATG ATGGCATATG CAGCAGCTAT ATGTGGATTT TTTTAGCCCT GCCTTCATAC GCTATTTATT TGCTTGGTAC TGTTTCTTTT GTCGATGCTC ACCCTGTTGT TTGGTGTTAC TTCTGCAGCC CTAGGCCTAC TAGATGGATT ACAAGGACCA CGACGGGGAT TACAAGGACC ACGACATTGA TTACAAGGAT GATGATGACA AGATGGCTCC GAAGAAGAAG AGGAAGGTTG GCATCCACGG GGTGCCAGCT GCTGACAAGA AGTACTCGAT CGGCCTCGAT ATTGGGACTA ACTCTGTTGG CTGGGCCGTG ATCACCGACG AGTACAAGGT GCCCTCAAAG AAGTTCAAGG TCCTGGGCAA CACCGATCGG CATTCCATCA AGAAGAATCT CATTGGCGCT CTCCTGTTCG ACAGCGGCGA GACGGCTGAG GCTACGCGGC TCAAGCGCAC CGCCCGCAGG CGGTACACGC GCAGGAAGAA TCGCATCTGC TACCTGCAGG AGATTTTCTC CAACGAGATG GCGAAGGTTG ACGATTCTTT CTTCCACAGG CTGGAGGAGT CATTCCTCGT GGAGGAGGAT AAGAAGCACG AGCGGCATCC AATCTTCGGC AACATTGTCG ACGAGGTTGC CTACCACGAG AAGTACCCTA CGATCTACCA TCTGCGGAAG AAGCTCGTGG ACTCCACAGA TAAGGCGGAC CTCCGCCTGA TCTACCTCGC TCTGGCCCAC ATGATTAAGT TCAGGGGCCA TTTCCTGATC GAGGGGGATC TCAACCCGGA CAATAGCGAT GTTGACAAGC TGTTCATCCA GCTCGTGCAG ACGTACAACC AGCTCTTCGA GGAGAACCCC ATTAATGCGT CAGGCGTCGA CGCGAAGGCT ATCCTGTCCG CTAGGCTCTC GAAGTCTCGG CGCCTCGAGA ACCTGATCGC CCAGCTGCCG GGCGAGAAGA AGAACGGCCT GTTCGGGAAT CTCATTGCGC TCAGCCTGGG GCTCACGCCC AACTTCAAGT CGAATTTCGA TCTCGCTGAG GACGCCAAGC TGCAGCTCTC CAAGGACACA TACGACGATG ACCTGGATAA CCTCCTGGCC CAGATCGGCG ATCAGTACGC GGACCTGTTC CTCGCTGCCA AGAATCTGTC GGACGCCATC CTCCTGTCTG ATATTCTCAG GGTGAACACC GAGATTACGA AGGCTCCGCT CTCAGCCTCC ATGATCAAGC GCTACGACGA GCACCATCAG GATCTGACCC TCCTGAAGGC GCTGGTCAGG CAGCAGCTCC CCGAGAAGTA CAAGGAGATC TTCTTCGATC AGTCGAAGAA CGGCTACGCT GGGTACATTG ACGGCGGGGC CTCTCAGGAG GAGTTCTACA AGTTCATCAA GCCGATTCTG GAGAAGATGG ACGGCACGGA GGAGCTGCTG GTGAAGCTCA ATCGCGAGGA CCTCCTGAGG AAGCAGCGGA CATTCGATAA CGGCAGCATC CCACACCAGA TTCATCTCGG GGAGCTGCAC GCTATCCTGA GGAGGCAGGA GGACTTCTAC CCTTTCCTCA AGGATAACCG CGAGAAGATC GAGAAGATTC TGACTTTCAG GATCCCGTAC TACGTCGGCC CACTCGCTAG GGGCAACTCC CGCTTCGCTT GGATGACCCG CAAGTCAGAG GAGACGATCA CGCCGTGGAA CTTCGAGGAG GTGGTCGACA AGGGCGCTAG CGCTCAGTCG TTCATCGAGA GGATGACGAA TTTCGACAAG AACCTGCCAA ATGAGAAGGT GCTCCCTAAG CACTCGCTCC TGTACGAGTA CTTCACAGTC TACAACGAGC TGACTAAGGT GAAGTATGTG ACCGAGGGCA TGAGGAAGCC GGCTTTCCTG TCTGGGGAGC AGAAGAAGGC CATCGTGGAC CTCCTGTTCA AGACCAACCG GAAGGTCACG GTTAAGCAGC TCAAGGAGGA CTACTTCAAG AAGATTGAGT GCTTCGATTC GGTCGAGATC TCTGGCGTTG AGGACCGCTT CAACGCCTCC CTGGGGACCT ACCACGATCT CCTGAAGATC ATTAAGGATA AGGACTTCCT GGACAACGAG GAGAATGAGG ATATCCTCGA GGACATTGTG CTGACACTCA CTCTGTTCGA GGACCGGGAG ATGATCGAGG AGCGCCTGAA GACTTACGCC CATCTCTTCG ATGACAAGGT CATGAAGCAG CTCAAGAGGA GGAGGTACAC CGGCTGGGGG AGGCTGAGCA GGAAGCTCAT CAACGGCATT CGGGACAAGC AGTCCGGGAA GACGATCCTC GACTTCCTGA AGAGCGATGG CTTCGCGAAC CGCAATTTCA TGCAGCTGAT TCACGATGAC AGCCTCACAT TCAAGGAGGA TATCCAGAAG GCTCAGGTGA GCGGCCAGGG GGACTCGCTG CACGAGCATA TCGCGAACCT CGCTGGCTCG CCAGCTATCA AGAAGGGGAT TCTGCAGACC GTGAAGGTTG TGGACGAGCT GGTGAAGGTC ATGGGCAGGC ACAAGCCTGA GAACATCGTC ATTGAGATGG CCCGGGAGAA TCAGACCACG CAGAAGGGCC AGAAGAACTC ACGCGAGAGG ATGAAGAGGA TCGAGGAGGG CATTAAGGAG CTGGGGTCCC AGATCCTCAA GGAGCACCCG GTGGAGAACA CGCAGCTGCA GAATGAGAAG CTCTACCTGT ACTACCTCCA GAATGGCCGC GATATGTATG TGGACCAGGA GCTGGATATT AACAGGCTCA GCGATTACGA CGTCGATCAT ATCGTTCCAC AGTCATTCCT GAAGGATGAC TCCATTGACA ACAAGGTCCT CACCAGGTCG GACAAGAACC GGGGCAAGTC TGATAATGTT CCTTCAGAGG AGGTCGTTAA GAAGATGAAG AACTACTGGC GCCAGCTCCT GAATGCCAAG CTGATCACGC AGCGGAAGTT CGATAACCTC ACAAAGGCTG AGAGGGGCGG GCTCTCTGAG CTGGACAAGG CGGGCTTCAT CAAGAGGCAG CTGGTCGAGA CACGGCAGAT CACTAAGCAC GTTGCGCAGA TTCTCGACTC ACGGATGAAC ACTAAGTACG ATGAGAATGA CAAGCTGATC CGCGAGGTGA AGGTCATCAC CCTGAAGTCA AAGCTCGTCT CCGACTTCAG GAAGGATTTC CAGTTCTACA AGGTTCGGGA GATCAACAAT TACCACCATG CCCATGACGC GTACCTGAAC GCGGTGGTCG GCACAGCTCT GATCAAGAAG TACCCAAAGC TCGAGAGCGA GTTCGTGTAC GGGGACTACA AGGTTTACGA TGTGAGGAAG ATGATCGCCA AGTCGGAGCA GGAGATTGGC AAGGCTACCG CCAAGTACTT CTTCTACTCT AACATTATGA ATTTCTTCAA GACAGAGATC ACTCTGGCCA ATGGCGAGAT CCGGAAGCGC CCCCTCATCG AGACGAACGG CGAGACGGGG GAGATCGTGT GGGACAAGGG CAGGGATTTC GCGACCGTCA GGAAGGTTCT CTCCATGCCA CAAGTGAATA TCGTCAAGAA GACAGAGGTC CAGACTGGCG GGTTCTCTAA GGAGTCAATT CTGCCTAAGC GGAACAGCGA CAAGCTCATC GCCCGCAAGA AGGACTGGGA TCCGAAGAAG TACGGCGGGT TCGACAGCCC CACTGTGGCC TACTCGGTCC TGGTTGTGGC GAAGGTTGAG AAGGGCAAGT CCAAGAAGCT CAAGAGCGTG AAGGAGCTGC TGGGGATCAC GATTATGGAG CGCTCCAGCT TCGAGAAGAA CCCGATCGAT TTCCTGGAGG CGAAGGGCTA CAAGGAGGTG AAGAAGGACC TGATCATTAA GCTCCCCAAG TACTCACTCT TCGAGCTGGA GAACGGCAGG AAGCGGATGC TGGCTTCCGC TGGCGAGCTG CAGAAGGGGA ACGAGCTGGC TCTGCCGTCC AAGTATGTGA ACTTCCTCTA CCTGGCCTCC CACTACGAGA AGCTCAAGGG CAGCCCCGAG GACAACGAGC AGAAGCAGCT GTTCGTCGAG CAGCACAAGC ATTACCTCGA CGAGATCATT GAGCAGATTT CCGAGTTCTC CAAGCGCGTG ATCCTGGCCG ACGCGAATCT GGATAAGGTC CTCTCCGCGT ACAACAAGCA CCGCGACAAG CCAATCAGGG AGCAGGCTGA GAATATCATT CATCTCTTCA CCCTGACGAA CCTCGGCGCC CCTGCTGCTT TCAAGTACTT CGACACAACT ATCGATCGCA AGAGGTACAC AAGCACTAAG GAGGTCCTGG ACGCGACCCT CATCCACCAG TCGATTACCG GCCTCTACGA GACGCGCATC GACCTGTCTC AGCTCGGGGG CGACAAGCGG CCAGCGGCGA CGAAGAAGGC GGGGCAGGCG AAGAAGAAGA AGTGAGCTCA GAGCTTTCGT TCGTATCATC GGTTTCGACA ACGTTCGTCA AGTTCAATGC ATCAGTTTCA TTGCGCACAC ACCAGAATCC TACTGAGTTT GAGTATTATG GCATTGGGAA AACTGTTTTT CTTGTACCAT TTGTTGTGCT TGTAATTTAC TGTGTTTTTT ATTCGGTTTT CGCTATCGAA CTGTGAAATG GAAATGGATG GAGAAGAGTT AATGAATGAT ATGGTCCTTT TGTTCATTCT CAAATTAATA TTATTTGTTT TTTCTCTTAT TTGTTGTGTG TTGAATTTGA AATTATAAGA GATATGCAAA CATTTTGTTT TGAGTAAAAA TGTGTCAAAT CGTGGCCTCT AATGACCGAA GTTAATATGA GGAGTAAAAC ACTTGTAGTT GTACCATTAT GCTTATTCAC TAGGCAACAA ATATATTTTC AGACCTAGAA AAGCTGCAAA TGTTACTGAA TACAAGTATG TCCTCTTGTG TTTTAGACAT TTATGAACTT TCCTTTATGT AATTTTCCAG AATCCTTGTC AGATTCTAAT CATTGCTTTA TAATTATAGT TATACTCATG GATTTGTAGT TGAGTATGAA AATATTTTTT AATGCATTTT ATGACTTGCC AATTGATTGA CAACGAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGCTAGA GCAGCTTGCC AACATGGTGG AGCACGACAC TCTCGTCTAC TCCAAGAATA TCAAAGATAC AGTCTCAGAA GACCAAAGGG CTATTGAGAC TTTTCAACAA AGGGTAATAT CGGGAAACCT CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTCATCAAA AGGACAGTAG AAAAGGAAGG TGGCACCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCT ATCGTTCAAG ATGCCTCTGC CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGACGT TCCAACCACG TCTTCAAAGC AAGTGGATTG ATGTGATAAC ATGGTGGAGC ACGACACTCT CGTCTACTCC AAGAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCTA TTGAGACTTT TCAACAAAGG GTAATATCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCAAAAGG ACAGTAGAAA AGGAAGGTGG CACCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC GTTCAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGGACACGCT GAAATCACCA GTCTCTCTCT ACAAATCTAT CTCTCTCGAG TCTACCATGA GCCCAGAACG ACGCCCGGCC GACATCCGCC GTGCCACCGA GGCGGACATG CCGGCGGTCT GCACCATCGT CAACCACTAC ATCGAGACAA GCACGGTCAA CTTCCGTACC GAGCCGCAGG AACCGCAGGA GTGGACGGAC GACCTCGTCC GTCTGCGGGA GCGCTATCCC TGGCTCGTCG CCGAGGTGGA CGGCGAGGTC GCCGGCATCG CCTACGCGGG CCCCTGGAAG GCACGCAACG CCTACGACTG GACGGCCGAG TCGACCGTGT ACGTCTCCCC CCGCCACCAG CGGACGGGAC TGGGCTCCAC GCTCTACACC CACCTGCTGA AGTCCCTGGA GGCACAGGGC TTCAAGAGCG TGGTCGCTGT CATCGGGCTG CCCAACGACC CGAGCGTGCG CATGCACGAG GCGCTCGGAT ATGCCCCCCG CGGCATGCTG CGGGCGGCCG GCTTCAAGCA CGGGAACTGG CATGACGTGG GTTTCTGGCA GCTGGACTTC AGCCTGCCGG TACCGCCCCG TCCGGTCCTG CCCGTCACCG AGATTTGACT CGAGTTTCTC CATAATAATG TGTGAGTAGT TCCCAGATAA GGGAATTAGG GTTCCTATAG GGTTTCGCTC ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT ACTTCTATCA ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTACTAAA ATCCAGATCC CCCGAATTAA TTCGGCGTTA ATTCAGTACA TTAAAAACGT CCGCAATGTG TTATTAAGTT GTCTAAGCGT CAATTTGTTT ACACCACAAT ATATCCTGCC ACCAGCCAGC CAACAGCTCC CCGACCGGCA GCTCGGCACA AAATCACCAC TCGATACAGG CAGCCCATCA GTCCGGGACG GCGTCAGCGG GAGAGCCGTT GTAAGGCGGC AGACTTTGCT CATGTTACCG ATGCTATTCG GAAGAACGGC AACTAAGCTG CCGGGTTTGA AACACGGATG ATCTCGCGGA GGGTAGCATG TTGATTGTAA CGATGACAGA GCGTTGCTGC CTGTGATCAC CGCGGTTTCA AAATCGGCTC CGTCGATACT ATGTTATACG CCAACTTTGA AAACAACTTT GAAAAAGCTG TTTTCTGGTA TTTAAGGTTT TAGAATGCAA GGAACAGTGA ATTGGAGTTC GTCTTGTTAT AATTAGCTTC TTGGGGTATC TTTAAATACT GTAGAAAAGA GGAAGGAAAT AATAAATGGC TAAAATGAGA ATATCACCGG AATTGAAAAA ACTGATCGAA AAATACCGCT GCGTAAAAGA TACGGAAGGA ATGTCTCCTG CTAAGGTATA TAAGCTGGTG GGAGAAAATG AAAACCTATA TTTAAAAATG ACGGACAGCC GGTATAAAGG GACCACCTAT GATGTGGAAC GGGAAAAGGA CATGATGCTA TGGCTGGAAG GAAAGCTGCC TGTTCCAAAG GTCCTGCACT TTGAACGGCA TGATGGCTGG AGCAATCTGC TCATGAGTGA GGCCGATGGC GTCCTTTGCT CGGAAGAGTA TGAAGATGAA CAAAGCCCTG AAAAGATTAT CGAGCTGTAT GCGGAGTGCA TCAGGCTCTT TCACTCCATC GACATATCGG ATTGTCCCTA TACGAATAGC TTAGACAGCC GCTTAGCCGA ATTGGATTAC TTACTGAATA ACGATCTGGC CGATGTGGAT TGCGAAAACT GGGAAGAAGA CACTCCATTT AAAGATCCGC GCGAGCTGTA TGATTTTTTA AAGACGGAAA AGCCCGAAGA GGAACTTGTC TTTTCCCACG GCGACCTGGG AGACAGCAAC ATCTTTGTGA AAGATGGCAA AGTAAGTGGC TTTATTGATC TTGGGAGAAG CGGCAGGGCG GACAAGTGGT ATGACATTGC CTTCTGCGTC CGGTCGATCA GGGAGGATAT CGGGGAAGAA CAGTATGTCG AGCTATTTTT TGACTTACTG GGGATCAAGC CTGATTGGGA GAAAATAAAA TATTATATTT TACTGGATGA ATTGTTTTAG TACCTAGAAT GCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGGGTGCCTT GATGTGGGCG CCGGCGGTCG AGTGGCGACG GCGCGGCTTG TCCGCGCCCT GGTAGATTGC CTGGCCGTAG GCCAGCCATT TTTGAGCGGC CAGCGGCCGC GATAGGCCGA CGCGAAGCGG CGGGGCGTAG GGAGCGCAGC GACCGAAGGG TAGGCGCTTT TTGCAGCTCT TCGGCTGTGC GCTGGCCAGA CAGTTATGCA CAGGCCAGGC GGGTTTTAAG AGTTTTAATA AGTTTTAAAG AGTTTTAGGC GGAAAAATCG CCTTTTTTCT CTTTTATATC AGTCACTTAC ATGTGTGACC GGTTCCCAAT GTACGGCTTT GGGTTCCCAA TGTACGGGTT CCGGTTCCCA ATGTACGGCT TTGGGTTCCC AATGTACGTG CTATCCACAG GAAACAGACC TTTTCGACCT TTTTCCCCTG CTAGGGCAAT TTGCCCTAGC ATCTGCTCCG TACATTAGGA ACCGGCGGAT GCTTCGCCCT CGATCAGGTT GCGGTAGCGC ATGACTAGGA TCGGGCCAGC CTGCCCCGCC TCCTCCTTCA AATCGTACTC CGGCAGGTCA TTTGACCCGA TCAGCTTGCG CACGGTGAAA CAGAACTTCT TGAACTCTCC GGCGCTGCCA CTGCGTTCGT AGATCGTCTT GAACAACCAT CTGGCTTCTG CCTTGCCTGC GGCGCGGCGT GCCAGGCGGT AGAGAAAACG GCCGATGCCG GGATCGATCA AAAAGTAATC GGGGTGAACC GTCAGCACGT CCGGGTTCTT GCCTTCTGTG ATCTCGCGGT ACATCCAATC AGCTAGCTCG ATCTCGATGT ACTCCGGCCG CCCGGTTTCG CTCTTTACGA TCTTGTAGCG GCTAATCAAG GCTTCACCCT CGGATACCGT CACCAGGCGG CCGTTCTTGG CCTTCTTCGT ACGCTGCATG GCAACGTGCG TGGTGTTTAA CCGAATGCAG GTTTCTACCA GGTCGTCTTT CTGCTTTCCG CCATCGGCTC GCCGGCAGAA CTTGAGTACG TCCGCAACGT GTGGACGGAA CACGCGGCCG GGCTTGTCTC CCTTCCCTTC CCGGTATCGG TTCATGGATT CGGTTAGATG GGAAACCGCC ATCAGTACCA GGTCGTAATC CCACACACTG GCCATGCCGG CCGGCCCTGC GGAAACCTCT ACGTGCCCGT CTGGAAGCTC GTAGCGGATC ACCTCGCCAG CTCGTCGGTC ACGCTTCGAC AGACGGAAAA CGGCCACGTC CATGATGCTG CGACTATCGC GGGTGCCCAC GTCATAGAGC ATCGGAACGA AAAAATCTGG TTGCTCGTCG CCCTTGGGCG GCTTCCTAAT CGACGGCGCA CCGGCTGCCG GCGGTTGCCG GGATTCTTTG CGGATTCGAT CAGCGGCCGC TTGCCACGAT TCACCGGGGC GTGCTTCTGC CTCGATGCGT TGCCGCTGGG CGGCCTGCGC GGCCTTCAAC TTCTCCACCA GGTCATCACC CAGCGCCGCG CCGATTTGTA CCGGGCCGGA TGGTTTGCGA CCGCTCACGC CGATTCCTCG GGCTTGGGGG TTCCAGTGCC ATTGCAGGGC CGGCAGACAA CCCAGCCGCT TACGCCTGGC CAACCGCCCG TTCCTCCACA CATGGGGCAT TCCACGGCGT CGGTGCCTGG TTGTTCTTGA TTTTCCATGC CGCCTCCTTT AGCCGCTAAA ATTCATCTAC TCATTTATTC ATTTGCTCAT TTACTCTGGT AGCTGCGCGA TGTATTCAGA TAGCAGCTCG GTAATGGTCT TGCCTTGGCG TACCGCGTAC ATCTTCAGCT TGGTGTGATC CTCCGCCGGC AACTGAAAGT TGACCCGCTT CATGGCTGGC GTGTCTGCCA GGCTGGCCAA CGTTGCAGCC TTGCTGCTGC GTGCGCTCGG ACGGCCGGCA CTTAGCGTGT TTGTGCTTTT GCTCATTTTC TCTTTACCTC ATTAACTCAA ATGAGTTTTG ATTTAATTTC AGCGGCCAGC GCCTGGACCT CGCGGGCAGC GTCGCCCTCG GGTTCTGATT CAAGAACGGT TGTGCCGGCG GCGGCAGTGC CTGGGTAGCT CACGCGCTGC GTGATACGGG ACTCAAGAAT GGGCAGCTCG TACCCGGCCA GCGCCTCGGC AACCTCACCG CCGATGCGCG TGCCTTTGAT CGCCCGCGAC ACGACAAAGG CCGCTTGTAG CCTTCCATCC GTGACCTCAA TGCGCTGCTT AACCAGCTCC ACCAGGTCGG CGGTGGCCCA TATGTCGTAA GGGCTTGGCT GCACCGGAAT CAGCACGAAG TCGGCTGCCT TGATCGCGGA CACAGCCAAG TCCGCCGCCT GGGGCGCTCC GTCGATCACT ACGAAGTCGC GCCGGCCGAT GGCCTTCACG TCGCGGTCAA TCGTCGGGCG GTCGATGCCG ACAACGGTTA GCGGTTGATC TTCCCGCACG GCCGCCCAAT CGCGGGCACT GCCCTGGGGA TCGGAATCGA CTAACAGAAC ATCGGCCCCG GCGAGTTGCA GGGCGCGGGC TAGATGGGTT GCGATGGTCG TCTTGCCTGA CCCGCCTTTC TGGTTAAGTA CAGCGATAAC CTTCATGCGT TCCCCTTGCG TATTTGTTTA TTTACTCATC GCATCATATA CGCAGCGACC GCATGACGCA AGCTGTTTTA CTCAAATACA CATCACCTTT TTAGACGGCG GCGCTCGGTT TCTTCAGCGG CCAAGCTGGC CGGCCAGGCC GCCAGCTTGG CATCAGACAA ACCGGCCAGG ATTTCATGCA GCCGCACGGT TGAGACGTGC GCGGGCGGCT CGAACACGTA CCCGGCCGCG ATCATCTCCG CCTCGATCTC TTCGGTAATG AAAAACGGTT CGTCCTGGCC GTCCTGGTGC GGTTTCATGC TTGTTCCTCT TGGCGTTCAT TCTCGGCGGC CGCCAGGGCG TCGGCCTCGG TCAATGCGTC CTCACGGAAG GCACCGCGCC GCCTGGCCTC GGTGGGCGTC ACTTCCTCGC TGCGCTCAAG TGCGCGGTAC AGGGTCGAGC GATGCACGCC AAGCAGTGCA GCCGCCTCTT TCACGGTGCG GCCTTCCTGG TCGATCAGCT CGCGGGCGTG CGCGATCTGT GCCGGGGTGA GGGTAGGGCG GGGGCCAAAC TTCACGCCTC GGGCCTTGGC GGCCTCGCGC CCGCTCCGGG TGCGGTCGAT GATTAGGGAA CGCTCGAACT CGGCAATGCC GGCGAACACG GTCAACACCA TGCGGCCGGC CGGCGTGGTG GTGTCGGCCC ACGGCTCTGC CAGGCTACGC AGGCCCGCGC CGGCCTCCTG GATGCGCTCG GCAATGTCCA GTAGGTCGCG GGTGCTGCGG GCCAGGCGGT CTAGCCTGGT CACTGTCACA ACGTCGCCAG GGCGTAGGTG GTCAAGCATC CTGGCCAGCT CCGGGCGGTC GCGCCTGGTG CCGGTGATCT TCTCGGAAAA CAGCTTGGTG CAGCCGGCCG CGTGCAGTTC GGCCCGTTGG TTGGTCAAGT CCTGGTCGTC GGTGCTGACG CGGGCATAGC CCAGCAGGCC AGCGGCGGCG CTCTTGTTCA TGGCGTAATG TCTCCGGTTC TAGTCGCAAG TATTCTACTT TATGCGACTA AAACACGCGA CAAGAAAACG CCAGGAAAAG GGCAGGGCGG CAGCCTGTCG CGTAACTTAG GACTTGTGCG ACATGTCGTT TTCAGAAGAC GGCTGCACTG AACGTCAGAA GCCGACTGCA CTATAGCAGC GGAGGGGTTG GATCAAAGTA CTTTGATCCC GAGGGGAACC CTGTGGTTGG CATGCACATA CAAATGGACG AACGGATAAA CCTTTTCACG CCCTTTTAAA TATCCGTTAT TCTAA
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