T7-VEE-OKS-iG-ΔG
$113.00
In stock
SKU
PL55529
100μg liquid plasmid(Instantly):$134
| name | T7-VEE-OKS-iG-ΔG | another name | aT7-VEE-OKS-iG-ΔG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression,Stem cell reprogramming | Fragment type | ORF,IPS |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGGGCGGCG CATGAGAGAA GCCCAGACCA ATTACCTACC CAAAATGGAG AAAGTTCACG TTGACATCGA GGAAGACAGC CCATTCCTCA GAGCTTTGCA GCGGAGCTTC CCGCAGTTTG AGGTAGAAGC CAAGCAGGTC ACTGATAATG ACCATGCTAA TGCCAGAGCG TTTTCGCATC TGGCTTCAAA ACTGATCGAA ACGGAGGTGG ACCCATCCGA CACGATCCTT GACATTGGAA GTGCGCCCGC CCGCAGAATG TATTCTAAGC ACAAGTATCA TTGTATCTGT CCGATGAGAT GTGCGGAAGA TCCGGACAGA TTGTATAAGT ATGCAACTAA GCTGAAGAAA AACTGTAAGG AAATAACTGA TAAGGAATTG GACAAGAAAA TGAAGGAGCT GGCCGCCGTC ATGAGCGACC CTGACCTGGA AACTGAGACT ATGTGCCTCC ACGACGACGA GTCGTGTCGC TACGAAGGGC AAGTCGCTGT TTACCAGGAT GTATACGCGG TTGACGGACC GACAAGTCTC TATCACCAAG CCAATAAGGG AGTTAGAGTC GCCTACTGGA TAGGCTTTGA CACCACCCCT TTTATGTTTA AGAACTTGGC TGGAGCATAT CCATCATACT CTACCAACTG GGCCGACGAA ACCGTGTTAA CGGCTCGTAA CATAGGCCTA TGCAGCTCTG ACGTTATGGA GCGGTCACGT AGAGGGATGT CCATTCTTAG AAAGAAGTAT TTGAAACCAT CCAACAATGT TCTATTCTCT GTTGGCTCGA CCATCTACCA CGAGAAGAGG GACTTACTGA GGAGCTGGCA CCTGCCGTCT GTATTTCACT TACGTGGCAA GCAAAATTAC ACATGTCGGT GTGAGACTAT AGTTAGTTGC GACGGGTACG TCGTTAAAAG AATAGCTATC AGTCCAGGCC TGTATGGGAA GCCTTCAGGC TATGCTGCTA CGATGCACCG CGAGGGATTC TTGTGCTGCA AAGTGACAGA CACATTGAAC GGGGAGAGGG TCTCTTTTCC CGTGTGCACG TATGTGCCAG CTACATTGTG TGACCAAATG ACTGGCATAC TGGCAACAGA TGTCAGTGCG GACGACGCGC AAAAACTGCT GGTTGGGCTC AACCAGCGTA TAGTCGTCAA CGGTCGCACC CAGAGAAACA CCAATACCAT GAAAAATTAC CTTTTGCCCG TAGTGGCCCA GGCATTTGCT AGGTGGGCAA AGGAATATAA GGAAGATCAA GAAGATGAAA GGCCACTAGG ACTACGAGAT AGACAGTTAG TCATGGGGTG TTGTTGGGCT TTTAGAAGGC ACAAGATAAC ATCTATTTAT AAGCGCCCGG ATACCCAAAC CATCATCAAA GTGAACAGCG ATTTCCACTC ATTCGTGCTG CCCAGGATAG GCAGTAACAC ATTGGAGATC GGGCTGAGAA CAAGAATCAG GAAAATGTTA GAGGAGCACA AGGAGCCGTC ACCTCTCATT ACCGCCGAGG ACGTACAAGA AGCTAAGTGC GCAGCCGATG AGGCTAAGGA GGTGCGTGAA GCCGAGGAGT TGCGCGCAGC TCTACCACCT TTGGCAGCTG ATGTTGAGGA GCCCACTCTG GAAGCCGATG TCGACTTGAT GTTACAAGAG GCTGGGGCCG GCTCAGTGGA GACACCTCGT GGCTTGATAA AGGTTACCAG CTACGATGGC GAGGACAAGA TCGGCTCTTA CGCTGTGCTT TCTCCGCAGG CTGTACTCAA GAGTGAAAAA TTATCTTGCA TCCACCCTCT CGCTGAACAA GTCATAGTGA TAACACACTC TGGCCGAAAA GGGCGTTATG CCGTGGAACC ATACCATGGT AAAGTAGTGG TGCCAGAGGG ACATGCAATA CCCGTCCAGG ACTTTCAAGC TCTGAGTGAA AGTGCCACCA TTGTGTACAA CGAACGTGAG TTCGTAAACA GGTACCTGCA CCATATTGCC ACACATGGAG GAGCGCTGAA CACTGATGAA GAATATTACA AAACTGTCAA GCCCAGCGAG CACGACGGCG AATACCTGTA CGACATCGAC AGGAAACAGT GCGTCAAGAA AGAACTAGTC ACTGGGCTAG GGCTCACAGG CGAGCTGGTG GATCCTCCCT TCCATGAATT CGCCTACGAG AGTCTGAGAA CACGACCAGC CGCTCCTTAC CAAGTACCAA CCATAGGGGT GTATGGCGTG CCAGGATCAG GCAAGTCTGG CATCATTAAA AGCGCAGTCA CCAAAAAAGA TCTAGTGGTG AGCGCCAAGA AAGAAAACTG TGCAGAAATT ATAAGGGACG TCAAGAAAAT GAAAGGGCTG GACGTCAATG CCAGAACTGT GGACTCAGTG CTCTTGAATG GATGCAAACA CCCCGTAGAG ACCCTGTATA TTGACGAAGC TTTTGCTTGT CATGCAGGTA CTCTCAGAGC GCTCATAGCC ATTATAAGAC CTAAAAAGGC AGTGCTCTGC GGGGATCCCA AACAGTGCGG TTTTTTTAAC ATGATGTGCC TGAAAGTGCA TTTTAACCAC GAGATTTGCA CACAAGTCTT CCACAAAAGC ATCTCTCGCC GTTGCACTAA ATCTGTGACT TCGGTCGTCT CAACCTTGTT TTACGACAAA AAAATGAGAA CGACGAATCC GAAAGAGACT AAGATTGTGA TTGACACTAC CGGCAGTACC AAACCTAAGC AGGACGATCT CATTCTCACT TGTTTCAGAG GGTGGGTGAA GCAGTTGCAA ATAGATTACA AAGGCAACGA AATAATGACG GCAGCTGCCT CTCAAGGGCT GACCCGTAAA GGTGTGTATG CCGTTCGGTA CAAGGTGAAT GAAAATCCTC TGTACGCACC CACCTCAGAA CATGTGAACG TCCTACTGAC CCGCACGGAG GACCGCATCG TGTGGAAAAC ACTAGCCGGC GACCCATGGA TAAAAACACT GACTGCCAAG TACCCTGGGA ATTTCACTGC CACGATAGAG GAGTGGCAAG CAGAGCATGA TGCCATCATG AGGCACATCT TGGAGAGACC GGACCCTACC GACGTCTTCC AGAATAAGGC AAACGTGTGT TGGGCCAAGG CTTTAGTGCC GGTGCTGAAG ACCGCTGGCA TAGACATGAC CACTGAACAA TGGAACACTG TGGATTATTT TGAAACGGAC AAAGCTCACT CAGCAGAGAT AGTATTGAAC CAACTATGCG TGAGGTTCTT TGGACTCGAT CTGGACTCCG GTCTATTTTC TGCACCCACT GTTCCGTTAT CCATTAGGAA TAATCACTGG GATAACTCCC CGTCGCCTAA CATGTACGGG CTGAATAAAG AAGTGGTCCG TCAGCTCTCT CGCAGGTACC CACAACTGCC TCGGGCAGTT GCCACTGGAA GAGTCTATGA CATGAACACT GGTACACTGC GCAATTATGA TCCGCGCATA AACCTAGTAC CTGTAAACAG AAGACTGCCT CATGCTTTAG TCCTCCACCA TAATGAACAC CCACAGAGTG ACTTTTCTTC ATTCGTCAGC AAATTGAAGG GCAGAACTGT CCTGGTGGTC GGGGAAAAGT TGTCCGTCCC AGGCAAAATG GTTGACTGGT TGTCAGACCG GCCTGAGGCT ACCTTCAGAG CTCGGCTGGA TTTAGGCATC CCAGGTGATG TGCCCAAATA TGACATAATA TTTGTTAATG TGAGGACCCC ATATAAATAC CATCACTATC AGCAGTGTGA AGACCATGCC ATTAAGCTTA GCATGTTGAC CAAGAAAGCT TGTCTGCATC TGAATCCCGG CGGAACCTGT GTCAGCATAG GTTATGGTTA CGCTGACAGG GCCAGCGAAA GCATCATTGG TGCTATAGCG CGGCAGTTCA AGTTTTCCCG GGTATGCAAA CCGAAATCCT CACTTGAAGA GACGGAAGTT CTGTTTGTAT TCATTGGGTA CGATCGCAAG GCCCGTACGC ACAATTCTTA CAAGCTTTCA TCAACCTTGA CCAACATTTA TACAGGTTCC AGACTCCACG AAGCCGGATG TGCACCCTCA TATCATGTGG TGCGAGGGGA TATTGCCACG GCCACCGAAG GAGTGATTAT AAATGCTGCT AACAGCAAAG GACAACCTGG CGGAGGGGTG TGCGGAGCGC TGTATAAGAA ATTCCCGGAA AGCTTCGATT TACAGCCGAT CGAAGTAGGA AAAGCGCGAC TGGTCAAAGG TGCAGCTAAA CATATCATTC ATGCCGTAGG ACCAAACTTC AACAAAGTTT CGGAGGTTGA AGGTGACAAA CAGTTGGCAG AGGCTTATGA GTCCATCGCT AAGATTGTCA ACGATAACAA TTACAAGTCA GTAGCGATTC CACTGTTGTC CACCGGCATC TTTTCCGGGA ACAAAGATCG ACTAACCCAA TCATTGAACC ATTTGCTGAC AGCTTTAGAC ACCACTGATG CAGATGTAGC CATATACTGC AGGGACAAGA AATGGGAAAT GACTCTCAAG GAAGCAGTGG CTAGGAGAGA AGCAGTGGAG GAGATATGCA TATCCGACGA CTCTTCAGTG ACAGAACCTG ATGCAGAGCT GGTGAGGGTG CATCCGAAGA GTTCTTTGGC TGGAAGGAAG GGCTACAGCA CAAGCGATGG CAAAACTTTC TCATATTTGG AAGGGACCAA GTTTCACCAG GCGGCCAAGG ATATAGCAGA AATTAATGCC ATGTGGCCCG TTGCAACGGA GGCCAATGAG CAGGTATGCA TGTATATCCT CGGAGAAAGC ATGAGCAGTA TTAGGTCGAA ATGCCCCGTC GAAGAGTCGG AAGCCTCCAC ACCACCTAGC ACGCTGCCTT GCTTGTGCAT CCATGCCATG ACTCCAGAAA GAGTACAGCG CCTAAAAGCC TCACGTCCAG AACAAATTAC TGTGTGCTCA TCCTTTCCAT TGCCGAAGTA TAGAATCACT GGTGTGCAGA AGATCCAATG CTCCCAGCCT ATATTGTTCT CACCGAAAGT GCCTGCGTAT ATTCATCCAA GGAAGTATCT CGTGGAAACA CCACCGGTAG ACGAGACTCC GGAGCCATCG GCAGAGAACC AATCCACAGA GGGGACACCT GAACAACCAC CACTTATAAC CGAGGATGAG ACCAGGACTA GAACGCCTGA GCCGATCATC ATCGAAGAGG AAGAAGAGGA TAGCATAAGT TTGCTGTCAG ATGGCCCGAC CCACCAGGTG CTGCAAGTCG AGGCAGACAT TCACGGGCCG CCCTCTGTAT CTAGCTCATC CTGGTCCATT CCTCATGCAT CCGACTTTGA TGTGGACAGT TTATCCATAC TTGACACCCT GGAGGGAGCT AGCGTGACCA GCGGGGCAAC GTCAGCCGAG ACTAACTCTT ACTTCGCAAA GAGTATGGAG TTTCTGGCGC GACCGGTGCC TGCGCCTCGA ACAGTATTCA GGAACCCTCC ACATCCCGCT CCGCGCACAA GAACACCGTC ACTTGCACCC AGCAGGGCCT GCTCGAGAAC CAGCCTAGTT TCCACCCCGC CAGGCGTGAA TAGGGTGATC ACTAGAGAGG AGCTCGAGGC GCTTACCCCG TCACGCACTC CTAGCAGGTC GGTCTCGAGA ACCAGCCTGG TCTCCAACCC GCCAGGCGTA AATAGGGTGA TTACAAGAGA GGAGTTTGAG GCGTTCGTAG CACAACAACA ATGACGGTTT GATGCGGGTG CATACATCTT TTCCTCCGAC ACCGGTCAAG GGCATTTACA ACAAAAATCA GTAAGGCAAA CGGTGCTATC CGAAGTGGTG TTGGAGAGGA CCGAATTGGA GATTTCGTAT GCCCCGCGCC TCGACCAAGA AAAAGAAGAA TTACTACGCA AGAAATTACA GTTAAATCCC ACACCTGCTA ACAGAAGCAG ATACCAGTCC AGGAAGGTGG AGAACATGAA AGCCATAACA GCTAGACGTA TTCTGCAAGG CCTAGGGCAT TATTTGAAGG CAGAAGGAAA AGTGGAGTGC TACCGAACCC TGCATCCTGT TCCTTTGTAT TCATCTAGTG TGAACCGTGC CTTTTCAAGC CCCAAGGTCG CAGTGGAAGC CTGTAACGCC ATGTTGAAAG AGAACTTTCC GACTGTGGCT TCTTACTGTA TTATTCCAGA GTACGATGCC TATTTGGACA TGGTTGACGG AGCTTCATGC TGCTTAGACA CTGCCAGTTT TTGCCCTGCA AAGCTGCGCA GCTTTCCAAA GAAACACTCC TATTTGGAAC CCACAATACG ATCGGCAGTG CCTTCAGCGA TCCAGAACAC GCTCCAGAAC GTCCTGGCAG CTGCCACAAA AAGAAATTGC AATGTCACGC AAATGAGAGA ATTGCCCGTA TTGGATTCGG CGGCCTTTAA TGTGGAATGC TTCAAGAAAT ATGCGTGTAA TAATGAATAT TGGGAAACGT TTAAAGAAAA CCCCATCAGG CTTACTGAAG AAAACGTGGT AAATTACATT ACCAAATTAA AAGGACCAAA AGCTGCTGCT CTTTTTGCGA AGACACATAA TTTGAATATG TTGCAGGACA TACCAATGGA CAGGTTTGTA ATGGACTTAA AGAGAGACGT GAAAGTGACT CCAGGAACAA AACATACTGA AGAACGGCCC AAGGTACAGG TGATCCAGGC TGCCGATCCG CTAGCAACAG CGTATCTGTG CGGAATCCAC CGAGAGCTGG TTAGGAGATT AAATGCGGTC CTGCTTCCGA ACATTCATAC ACTGTTTGAT ATGTCGGCTG AAGACTTTGA CGCTATTATA GCCGAGCACT TCCAGCCTGG GGATTGTGTT CTGGAAACTG ACATCGCGTC GTTTGATAAA AGTGAGGACG ACGCCATGGC TCTGACCGCG TTAATGATTC TGGAAGACTT AGGTGTGGAC GCAGAGCTGT TGACGCTGAT TGAGGCGGCT TTCGGCGAAA TTTCATCAAT ACATTTGCCC ACTAAAACTA AATTTAAATT CGGAGCCATG ATGAAATCTG GAATGTTCCT CACACTGTTT GTGAACACAG TCATTAACAT TGTAATCGCA AGCAGAGTGT TGAGAGAACG GCTAACCGGA TCACCATGTG CAGCATTCAT TGGAGATGAC AATATCGTGA AAGGAGTCAA ATCGGACAAA TTAATGGCAG ACAGGTGCGC CACCTGGTTG AATATGGAAG TCAAGATTAT AGATGCTGTG GTGGGCGAGA AAGCGCCTTA TTTCTGTGGA GGGTTTATTT TGTGTGACTC CGTGACCGGC ACAGCGTGCC GTGTGGCAGA CCCCCTAAAA AGGCTGTTTA AGCTTGGCAA ACCTCTGGCA GCAGACGATG AACATGATGA TGACAGGAGA AGGGCATTGC ATGAAGAGTC AACACGCTGG AACCGAGTGG GTATTCTTTC AGAGCTGTGC AAGGCAGTAG AATCAAGGTA TGAAACCGTA GGAACTTCCA TCATAGTTAT GGCCATGACT ACTCTAGCTA GCAGTGTTAA ATCATTCAGC TACCTGAGAG GGGCCCCTAT AACTCTCTAC GGCTAACCTG AATGGACTAC GACATAGTCT AGTCCGCCAA GTCTAGCATA TGGGCGCGTG AATTCGCCAC CATGGCGGGA CACCTGGCTT CGGATTTCGC CTTCTCGCCC CCTCCAGGTG GTGGAGGTGA TGGGCCAGGG GGGCCGGAGC CGGGCTGGGT TGATCCTCGG ACCTGGCTAA GCTTCCAAGG CCCTCCTGGA GGGCCAGGAA TCGGGCCGGG GGTTGGGCCA GGCTCTGAGG TGTGGGGGAT TCCCCCATGC CCCCCGCCGT ATGAGTTCTG TGGGGGGATG GCGTACTGTG GGCCCCAGGT TGGAGTGGGG CTAGTGCCCC AAGGCGGCTT GGAGACCTCT CAGCCTGAGG GCGAAGCAGG AGTCGGGGTG GAGAGCAACT CCGATGGGGC CTCCCCGGAG CCCTGCACCG TCACCCCTGG TGCCGTGAAG CTGGAGAAGG AGAAGCTGGA GCAAAACCCG GAGGAGTCCC AGGACATCAA AGCTCTGCAG AAAGAACTCG AGCAATTTGC CAAGCTCCTG AAGCAGAAGA GGATCACCCT GGGATATACA CAGGCCGATG TGGGGCTCAC CCTGGGGGTT CTATTTGGGA AGGTATTCAG CCAAACGACC ATCTGCCGCT TTGAGGCTCT GCAGCTTAGC TTCAAGAACA TGTGTAAGCT GCGGCCCTTG CTGCAGAAGT GGGTGGAGGA AGCTGACAAC AATGAAAATC TTCAGGAGAT ATGCAAAGCA GAAACCCTCG TGCAGGCCCG AAAGAGAAAG CGAACCAGTA TCGAGAACCG AGTGAGAGGC AACCTGGAGA ATTTGTTCCT GCAGTGCCCG AAACCCACAC TGCAGCAGAT CAGCCACATC GCCCAGCAGC TTGGGCTCGA GAAGGATGTG GTCCGAGTGT GGTTCTGTAA CCGGCGCCAG AAGGGCAAGC GATCAAGCAG CGACTATGCA CAACGAGAGG ATTTTGAGGC TGCTGGGTCT CCTTTCTCAG GGGGACCAGT GTCCTTTCCT CTGGCCCCAG GGCCCCATTT TGGTACCCCA GGCTATGGGA GCCCTCACTT CACTGCACTG TACTCCTCGG TCCCTTTCCC TGAGGGGGAA GCCTTTCCCC CTGTCTCCGT CACCACTCTG GGCTCTCCCA TGCATTCAAA CTCTAGTGAG GGCAGAGGAA GTCTGCTAAC ATGCGGTGAC GTCGAGGAGA ATCCTGGCCC ACAATTGATG GCTGTCAGCG ACGCGCTGCT CCCATCTTTC TCCACGTTCG CGTCTGGCCC GGCGGGAAGG GAGAAGACAC TGCGTCAAGC AGGTGCCCCG AATAACCGCT GGCGGGAGGA GCTCTCCCAC ATGAAGCGAC TTCCCCCAGT GCTTCCCGGC CGCCCCTATG ACCTGGCGGC GGCGACCGTG GCCACAGACC TGGAGAGCGG CGGAGCCGGT GCGGCTTGCG GCGGTAGCAA CCTGGCGCCC CTACCTCGGA GAGAGACCGA GGAGTTCAAC GATCTCCTGG ACCTGGACTT TATTCTCTCC AATTCGCTGA CCCATCCTCC GGAGTCAGTG GCCGCCACCG TGTCCTCGTC AGCGTCAGCC TCCTCTTCGT CGTCGCCGTC GAGCAGCGGC CCTGCCAGCG CGCCCTCCAC CTGCAGCTTC ACCTATCCGA TCCGGGCCGG GAACGACCCG GGCGTGGCGC CGGGCGGCAC GGGCGGAGGC CTCCTCTATG GCAGGGAGTC CGCTCCCCCT CCGACGGCTC CCTTCAACCT GGCGGACATC AACGACGTGA GCCCCTCGGG CGGCTTCGTG GCCGAGCTCC TGCGGCCAGA ATTGGACCCG GTGTACATTC CGCCGCAGCA GCCGCAGCCG CCAGGTGGCG GGCTGATGGG CAAGTTCGTG CTGAAGGCGT CGCTGAGCGC CCCTGGCAGC GAGTACGGCA GCCCGTCGGT CATCAGCGTC AGCAAAGGCA GCCCTGACGG CAGCCACCCG GTGGTGGTGG CGCCCTACAA CGGCGGGCCG CCGCGCACGT GCCCCAAGAT CAAGCAGGAG GCGGTCTCTT CGTGCACCCA CTTGGGCGCT GGACCCCCTC TCAGCAATGG CCACCGGCCG GCTGCACACG ACTTCCCCCT GGGGCGGCAG CTCCCCAGCA GGACTACCCC GACCCTGGGT CTTGAGGAAG TGCTGAGCAG CAGGGACTGT CACCCTGCCC TGCCGCTTCC TCCCGGCTTC CATCCCCACC CGGGGCCCAA TTACCCATCC TTCCTGCCCG ATCAGATGCA GCCGCAAGTC CCGCCGCTCC ATTACCAAGA GCTCATGCCA CCCGGTTCCT GCATGCCAGA GGAGCCCAAG CCAAAGAGGG GAAGACGATC GTGGCCCCGG AAAAGGACCG CCACCCACAC TTGTGATTAC GCGGGCTGCG GCAAAACCTA CACAAAGAGT TCCCATCTCA AGGCACACCT GCGAACCCAC ACAGGTGAGA AACCTTACCA CTGTGACTGG GACGGCTGTG GATGGAAATT CGCCCGCTCA GATGAACTGA CCAGGCACTA CCGTAAACAC ACGGGGCACC GCCCGTTCCA GTGCCAAAAA TGCGACCGAG CATTTTCCAG GTCGGACCAC CTCGCCTTAC ACATGAAGAG GCATTTTTCT AGACAATGTA CTAACTACGC TTTGTTGAAA CTCGCTGGCG ATGTTGAAAG TAACCCCGGT CCTGGCGCGC CCATGTACAA CATGATGGAG ACGGAGCTGA AGCCGCCGGG CCCGCAGCAA ACTTCGGGGG GCGGCGGCGG CAACTCCACC GCGGCGGCGG CCGGCGGCAA CCAGAAAAAC AGCCCGGACC GCGTCAAGCG GCCCATGAAT GCCTTCATGG TGTGGTCCCG CGGGCAGCGG CGCAAGATGG CCCAGGAGAA CCCCAAGATG CACAACTCGG AGATCAGCAA GCGCCTGGGC GCCGAGTGGA AACTTTTGTC GGAGACGGAG AAGCGGCCGT TCATCGACGA GGCTAAGCGG CTGCGAGCGC TGCACATGAA GGAGCACCCG GATTATAAAT ACCGGCCCCG GCGGAAAACC AAGACGCTCA TGAAGAAGGA TAAGTACACG CTGCCCGGCG GGCTGCTGGC CCCCGGCGGC AATAGCATGG CGAGCGGGGT CGGGGTGGGC GCCGGCCTGG GCGCGGGCGT GAACCAGCGC ATGGACAGTT ACGCGCACAT GAACGGCTGG AGCAACGGCA GCTACAGCAT GATGCAGGAC CAGCTGGGCT ACCCGCAGCA CCCGGGCCTC AATGCGCACG GCGCAGCGCA GATGCAGCCC ATGCACCGCT ACGACGTGAG CGCCCTGCAG TACAACTCCA TGACCAGCTC GCAGACCTAC ATGAACGGCT CGCCCACCTA CAGCATGTCC TACTCGCAGC AGGGCACCCC TGGCATGGCT CTTGGCTCCA TGGGTTCGGT GGTCAAGTCC GAGGCCAGCT CCAGCCCCCC TGTGGTTACC TCTTCCTCCC ACTCCAGGGC GCCCTGCCAG GCCGGGGACC TCCGGGACAT GATCAGCATG TATCTCCCCG GCGCCGAGGT GCCGGAACCC GCCGCCCCCA GCAGACTTCA CATGTCCCAG CACTACCAGA GCGGCCCGGT GCCCGGCACG GCCATTAACG GCACACTGCC CCTCTCACAC ATGTGAGCGG CCATCGATAA CTAACTTAAG CTAGCAACGG TTTCCCTCTA GCGGGATCAA TTCCGCCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATAATACC AATTCGCCAC CATGGCAGAG GCCCGCACAT CCCTGTCTGC CCACTGTCGG GGCCCGCTGG CCACTGGCCT GCACCCAGAC CTGGACCTCC CGGGCCGAAG CCTCGCCACC CCTGCGCCTT CCTGCTACCT TCTGGGCAGC GAACCCAGCT CTGGCCTGGG CCTCCAGCCC GAGACCCACC TCCCCGAGGG CAGCCTGAAG CGGTGCTGCG TCTTGGGCCT ACCCCCCACC TCCCCAGCCT CCTCCTCACC CTGTGCCTCC TCCGACGTCA CCTCCATCAT CCGCTCCTCC CAGACGTCTC TGGTCACCTG TGTAAATGGA CTCCGGAGCC CCCCTCTGAC GGGAGATCTG GGGGGCCCTT CCAAGCGGGC CCGGCCTGGC CCTGCATCGA CGGACAGCCA TGAGGGCAGC TTGCAACTTG AAGCCTGCCG GAAGGCGAGC TTCCTGAAGC AGGAACCCGC GGATGAGTTT TCAGAGCTCT TTGGGCCTCA CCAGCAGGGC CTGCCGCCCC CCTATCCCCT GTCTCAGTTG CCGCCTGGCC CAAGCCTTGG AGGCCTGGGG CTGGGCCTGG CAGGCAGGGT GGTGGCCGGG CGGCAGGCGT GCCGCTGGGT GGACTGCTGT GCAGCCTATG AGCAGCAGGA GGAGCTGGTG CGGCACATCG AGAAGAGCCA CATCGACCAG CGCAAGGGCG AGGACTTCAC CTGCTTCTGG GCTGGCTGCG TGCGCCGCTA CAAGCCCTTC AACGCCCGCT ACAAGCTGCT CATCCACATG CGAGTGCACT CGGGCGAGAA GCCCAACAAG TGCATGTTTG AAGGCTGCAG CAAGGCCTTC TCACGGCTGG AGAACCTCAA GATCCACCTG AGGAGCCACA CGGGCGAGAA GCCGTACCTG TGCCAGCACC CGGGTTGCCA GAAGGCCTTC AGCAACTCCA GCGACCGCGC CAAGCACCAG CGCACCCACC TAGACACGAA GCCGTACGCC TGTCAGATCC CTGGCTGCTC CAAGCGCTAC ACAGACCCCA GCTCCCTCCG CAAGCACGTC AAGGCCCATT CAGCCAAAGA GCAGCAGGTG CGTAAGAAGC TGCATGCGGG CCCTGACACC GAGGCCGACG TCCTGACCGA GTGTCTGGTC CTGCAGCAGC TCCACACGTC CACACAGCTG GCTGCCAGCG ACGGCAAGGG TGGCTGTGGC CTGGGCCAGG AGCTGCTCCC AGGTGTGTAT CCTGGCTCCA TCACCCCCCA TAACGGACTT GCATCGGGCC TCCTGCCCCC AGCGCACGAC GTACCTTCCA GGCACCACCC GCTGGATGCC ACCACCAGTT CCCACCACCA TCTGTCCCCT CTGCCCATGG CTGAGAGCAC CCGGGATGGG TTGGGGCCCG GCCTCCTCTC ACCAATAGTC AGCCCCCTGA AGGGGCTGGG GCCACCGCCG CTGCCCCCAT CCTCTCAGAG CCATTCTCCG GGGGGCCAGC CCTTCCCCAC ACTCCCCAGC AAGCCGTCCT ACCCACCCTT CCAGAGCCCT CCACCCCCGC CTCTGCCCAG CCCACAAGGT TACCAGGGCA GTTTCCACTC CATCCAGAGT TGCTTCCCCT ATGGCGACTG CTACCGGATG GCTGAACCAG CAGCCGGTGG GGACGGACTG GTCGGGGAGA CCCACGGTTT CAACCCCCTG CGGCCCAATG GCTACCACAG CCTCAGCACG CCCTTGCCTG CCACAGGCTA TGAGGCCCTG GCTGAGGCCT CATGCCCCAC AGCGCTGCCA CAGCAGCCAT CTGAAGATGT GGTGTCCAGC GGCCCCGAGG ACTGTGGCTT CTTCCCCAAT GGAGCCTTTG ACCACTGCCT GGGCCACATC CCCTCCATCT ACACAGACAC CTGAGCGGCC GCAACTAACT TAAGCTAGCA ACGGTTTCCC TCTAGCGGGA TCAATTCCGC CCCCCCCCCC TAACGTTACT GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT ATATGTTATT TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC CTGTCTTCTT GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC TGTTGAATGT CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG ATGCCCAGAA GGTACCCCAT TGTATGGGAT CTGATCTGGG GCCTCGGTGC ACATGCTTTA CATGTGTTTA GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATAA TACCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGAATTG GCAAGCTGCT TACATAGAAC TCGCGGCGAT TGGCATGCCG CCTTAAAATT TTTATTTTAT TTTTCTTTTC TTTTCCGAAT CGGATTTTGT TTTTAATATT TCAAAAAAAA AAAAAAAAAA AAAAAAAACG CGTCGAGGGG AATTAATTCT TGAAGACGAA AGGGCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GAGCTCTAAT ACGACTCACT ATA
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