pX333-U6-Ptk7-gRNA1-CBH-Cre-HA
$84.00
In stock
SKU
PL55516
100μg liquid plasmid(Instantly):$105
| name | pX333-U6-Ptk7-gRNA1-CBH-Cre-HA | another name | apX333-U6-Ptk7-gRNA1-CBH-Cre-HA |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTTTTGC TCACATGCGC CATTCTCGAG CATGCGCCAT TCTCGAGCAT GCTCGAGAAT GGCGCATGTG AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACCGG GTCTTCGAGA AGACCTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGCTCTA GAGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGCGTCTACC GTTGCATCGG TCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTTT TAGCGCGTGC GCCAATTCTG CAGACAAATG GGGTACCCTC CATCACTAGG GGTTCCTGCG GCCGCACGCG TGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG CTGTTAGAGA GATAATTGGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACACC GGAAGAGCGA GCTCTTCTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT TCTAGAGGTA CCAGGTCTTG AAAGGAGTGG GAATTGGCTC CGGTGCCCGT CAGTCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTGTGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT TTAATTATTT TGTGCAGCGA TGGGGGCGGG GGGGGGGGGG GGGCGCGCGC CAGGCGGGGC GGGGCGGGGC GAGGGGCGGG GCGGGGCGAG GCGGAGAGGT GCGGCGGCAG CCAATCAGAG CGGCGCGCTC CGAAAGTTTC CTTTTATGGC GAGGCGGCGG CGGCGGCGGC CCTATAAAAA GCGAAGCGCG CGGCGGGCGG GAGTCGCTGC GCGCTGCCTT CGCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTC CGGGCTGTAA TTAGCTGAGC AAGAGGTAAG GGTTTAAGGG ATGGTTGGTT GGTGGGGTAT TAATGTTTAA TTACCTGGAG CACCTGCCTG AAATCACTTT TTTTCAGGTT GGACCGGTGC CACCATGCCC AAGAAGAAGA GGAAGGTGTC CAATCTCCTG ACTGTTCACC AGAACCTCCC TGCGCTGCCA GTAGATGCCA CTAGCGATGA GGTCAGGAAA AATCTCATGG ATATGTTTAG GGATAGACAG GCGTTTTCTG AACACACCTG GAAAATGCTG CTTAGCGTGT GCCGATCCTG GGCAGCCTGG TGTAAGCTGA ACAATCGCAA ATGGTTCCCC GCCGAGCCGG AGGACGTGCG CGATTACCTG CTGTATCTCC AGGCAAGAGG GCTGGCTGTC AAGACTATCC AGCAGCACTT GGGCCAACTG AATATGCTGC ATCGACGCAG CGGGCTCCCC CGGCCTAGCG ATTCAAACGC AGTCTCCCTT GTTATGAGGA GAATTAGAAA GGAAAACGTA GATGCGGGTG AGAGGGCTAA GCAGGCTCTC GCTTTTGAGC GGACTGATTT CGACCAGGTC AGATCCCTGA TGGAGAACAG CGATCGGTGC CAGGACATCA GGAACCTCGC ATTTCTGGGA ATTGCATATA ACACACTTCT GCGCATAGCT GAGATCGCCC GGATCAGAGT GAAAGACATC AGTCGAACGG ACGGCGGCCG GATGCTTATT CATATTGGAC GCACAAAGAC ATTGGTCAGC ACCGCTGGCG TTGAAAAGGC CTTGTCCCTG GGCGTAACGA AGCTGGTGGA AAGATGGATC TCAGTGTCCG GCGTGGCTGA CGACCCTAAT AATTACTTGT TCTGTCGAGT GAGAAAAAAC GGAGTCGCCG CGCCCTCTGC CACCAGCCAA TTGAGTACAC GGGCCCTTGA AGGGATCTTT GAGGCAACCC ACCGACTCAT ATACGGAGCC AAGGATGACA GTGGCCAGAG GTATCTCGCC TGGTCAGGTC ATTCTGCTAG GGTGGGGGCC GCACGAGACA TGGCGCGGGC AGGAGTCTCC ATACCAGAGA TTATGCAAGC TGGAGGTTGG ACAAATGTGA ACATCGTTAT GAACTATATC CGCAATCTTG ACTCTGAAAC CGGGGCCATG GTGAGACTGC TCGAAGATGG TGACTACCCA TACGATGTTC CAGATTACGC TTAAGAATTC CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG AGAATAGCAG GCATGCTGGG GAGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CTGCCTGCAG GGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCTTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACTCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGTCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGAAGCCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT
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