pGL4.11-SHMT1(human)-Promoter-Fluc-hPEST
$84.00
In stock
SKU
PL55241
100μg liquid plasmid(Instantly):$105
| name | pGL4.11-SHMT1(human)-Promoter-Fluc-hPEST | another name | apGL4.11-SHMT1(human)-Promoter-Fluc-hPEST |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCC GCCCGGTTTT TATCATTCTT ATAATAATTA TTGTTATCAT TTGACAAATA AAAAAATTTA TGACGCAGCC TCAGGTGGTC CTGACATGTG GCCCCGGTTT TTATCATTCT TAACAATAGG TAATGTTCAT TGAGTCCTTA TTGTGTGTCA GTATTTTATT TATCTTATTT TATTCTTCTT GAGACGTTGT CTCGCTCCGT CCCCCAGGCT GGAGTGCAGT GGCGCGATCT CGACTCACTG CAACCTCCGC CTCCTGGGAG GCAACATAGT TAGACCCCCA TCTCTACTTC AACCTGGGGG ACAGAGTGAG ACCCTGTCTC AAAAAAATAA AAATAAAAAA TAACAAAAAT AATAATAATA AAGTGTCTGC TCTTGGATTA TACACCTACA GAGTAATAAT GTTGCAGTTT GGACGCACAT TTGTCCTACT TTAAATGACC ACCCTGCAAA CTAAAAGTAG TACCATGAAC AAAATTCACT CATAATCCCA CGGCCCAAGC GACAGGCTTA AGTGAGGAAG GCCCTGTGTA GTCTTCCTGG AAATCTTGGT CCACACACCA GCCCTGCAGC CAAAGCGGGT CAGTTCTGCA GGTCCCTGCC CCAGGTGCGC AGCTGCACTA CGCGGCCTCC GGCCCGCCCC GGAGAAACAG GCCCTGCCCG CCGCTGCGCC GCAGCCAATA GCGCGGGTGC GTTTGGTGGC GGCCCATGCT TGGCTGCGCT CTCTGATTGG GCGCCTCCGG GGGCGGGGCC AAGCCCAAGC CTGGCGCGCA GAGTGCACCT TCCTGAGCTC GAGCGGTCCA GCGCCAAGTT CGGGGTTTGG GGTTGGAGCG GCTGGTCACG TGGCTGGCCC GCGGCGGTGC GCGGGGCGTT GGGTCAGCGG GTCTGGGACT GGTGGCACCG GCGGCGGCGT AGGACGGAGG CGTCGCTAGG TACGTGCGCG GGCCGTGTTC CGGTAGGTGG GCGGCTTCGG GTCCGAGGGC CGCGGCGTCC CAGAGCCCGG GGGGTGCTTC GGCGTCCTCG CTGTCCCCCG CCGTACCCCA CCCTCTGCAG AAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG CCGTGAATTC TCACGGCTTC CCTCCCGAGG TGGAGGAGCA GGCCGCCGGC ACCCTGCCCA TGAGCTGCGC CCAGGAGAGC GGCATGGATA GACACCCTGC TGCTTGCGCC AGCGCCAGGA TCAACGTCTA AGGCCGCGAC TCTAGAGTCG GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGATCCGT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGCG GCCGCAAATG CTAAACCACT GCAGTGGTTA CCAGTGCTTG ATCAGTGAGG CACCGATCTC AGCGATCTGC CTATTTCGTT CGTCCATAGT GGCCTGACTC CCCGTCGTGT AGATCACTAC GATTCGTGAG GGCTTACCAT CAGGCCCCAG CGCAGCAATG ATGCCGCGAG AGCCGCGTTC ACCGGCCCCC GATTTGTCAG CAATGAACCA GCCAGCAGGG AGGGCCGAGC GAAGAAGTGG TCCTGCTACT TTGTCCGCCT CCATCCAGTC TATGAGCTGC TGTCGTGATG CTAGAGTAAG AAGTTCGCCA GTGAGTAGTT TCCGAAGAGT TGTGGCCATT GCTACTGGCA TCGTGGTATC ACGCTCGTCG TTCGGTATGG CTTCGTTCAA CTCTGGTTCC CAGCGGTCAA GCCGGGTCAC ATGATCACCC ATATTATGAA GAAATGCAGT CAGCTCCTTA GGGCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCGGTGT TGTCGCTCAT GGTAATGGCA GCACTACACA ATTCTCTTAC CGTCATGCCA TCCGTAAGAT GCTTTTCCGT GACCGGCGAG TACTCAACCA AGTCGTTTTG TGAGTAGTGT ATACGGCGAC CAAGCTGCTC TTGCCCGGCG TCTATACGGG ACAACACCGC GCCACATAGC AGTACTTTGA AAGTGCTCAT CATCGGGAAT CGTTCTTCGG GGCGGAAAGA CTCAAGGATC TTGCCGCTAT TGAGATCCAG TTCGATATAG CCCACTCTTG CACCCAGTTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCGGGGTGT GCAAAAACAG GCAAGCAAAA TGCCGCAAAG AAGGGAATGA GTGCGACACG AAAATGTTGG ATGCTCATAC TCGTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA CTAGTACGTC TCTCAAGGAT AAGTAAGTAA TATTAAGGTA CGGGAGGTAT TGGACAGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG TACTAACATA CGCTCTCCAT CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT TTCTCT
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