pETDuet1-Promoter-mcjB-mcjC-mcjD(coli)-T7-msyB-mcjA(coli)
$113.00
In stock
SKU
PL55179
100μg liquid plasmid(Instantly):$134
| name | pETDuet1-Promoter-mcjB-mcjC-mcjD(coli)-T7-msyB-mcjA(coli) | another name | apETDuet1-Promoter-mcjB-mcjC-mcjD(coli)-T7-msyB-mcjA(coli) |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | T7ter：TGCTAGTTATTGCTCAGCGG | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCTTTTTGA TGCAATCCGC TTTGCTTCTG ACTATAATAG TCAGGGTAAT TGAGTGTAAA GGCATAACTA CAGGAGGGAG TGTGCAAAAT GATCCGTTAC TGCTTAACCA GTTATAGAGA GGATCTTGTT ATCCTGGATA TAATTAATGA TAGTTTCAGC ATAGTGCCTG ACGCAGGTAG CTTGCTAAAA GAAAGAGATA AATTGCTTAA AGAATTCCCA CAACTATCTT ACTTTTTTGA CAGTGAATAT CATATTGGAA GTGTTTCTCG TAATAGTGAC ACTTCTTTTC TTGAAGAACG CTGGTTTCTA CCAGAACCTG ACAAAACATT ATATAAGTGT TCTCTATTTA AACGATTTAT ATTATTACTC AAAGTCTTTT ACTATAGCTG GAATATTGAA AAAAAAGGGA TGGCATGGAT TTTCATAAGT AATAAAAAAG AGAATAGGCT ATACTCCTTG AATGAAGAGC ATCTTATCCG GAAAGAAATT AGTAATCTTT CCATTATCTT TCATCTTAAT ATTTTTAAAT CTGACTGTCT TACCTATTCA TACGCACTAA AAAGAATTCT TAATTCCAGA AATATTGATG CTCATCTTGT TATTGGTGTA AGGACACAAC CTTTTTATAG CCACTCTTGG GTGGAGGTTG GGGGACAAGT TATCAATGAT GCTCCCAATA TGCGGGATAA ATTATCTGTT ATTGCAGAGA TATAGTTATG GAAATATTTA ATGTCAAGTT AAATGATACT TCAATTAGAA TTATTTTCTG TAAAACGCTT TCTGCCTTCC GGACAGAAAA TACCATCGTT ATGCTCAAAG GAAAAGCAGT TTCAAATGGC AAACCTGTAT CCACAGAGGA GATTGCCAGA GTAGTGGAAG AAAAAGGTGT TTCAGAAGTA ATAGAAAATT TAGATGGTGT TTTCTGTATC CTAATTTATC ATTTTAATGA TCTCCTTATA GGGAAAAGCA TTCAATCAGG CCCCGCTCTA TTTTATTGTA AAAAGAATAT GGATATTTTT GTTTCGGATA AAATTTCTGA TATCAAATTT TTGAATCCAG ATATGACATT CAGTCTAAAT ATAACAATGG CAGAACATTA TCTGTCAGGA AATCGAATAG CAACCCAGGA ATCACTAATC ACTGGCATTT ACAAAGTAAA TAATGGTGAG TTTATAAAAT TTAATAATCA GTTGAAACCT GTGCTACTTC GTGATGAGTT TAGTATTACC AAAAAGAACA ATTCAACTAT CGACAGTATC ATTGATAATA TTGAGATGAT GCGGGATAAT AGAAAAATAG CCCTATTATT TTCCGGAGGA TTGGATTCTG CATTAATTTT TCACACACTT AAAGAATCAG GTAACAAATT CTGCGCTTAT CATTTTTTTT CTGATGAATC TGATGACAGT GAAAAGTATT TTGCTAAGGA ATACTGTTCA AAATATGGAG TTGATTTTAT ATCTGTTAAT AAAAACATCA ACTTTAATGA AAAACTTTAT TTCAATTTAA ATCCTAATAG TCCGGACGAA ATCCCTTTGA TATTTGAACA GACAGATGAA GAAGGTGAAG GTCAGCCCCC CATAGACGAT GATTTATTAT ATCTATGTGG TCACGGTGGA GATCATATTT TCGGACAAAA TCCTTCAGAA CTTTTTGGCA TTGATGCATA TCGAAGTCAT GGCTTGATGT TTATGCATAA AAAAATAGTA GAATTTTCCA ATCTCAAGGG AAAGAGATAT AAAGATATCA TATTTTCAAA TATTTCCGCA TTCATTAATA CATCCAACGG ATGTTCTCCA GCAAAGCAAG AGCACGTATC AGATATGAAA CTTGCCTCTG CTCAGTTTTT TGCAACTGAT TATACAGGAA AAATTAATAA ACTAACTCCA TTCCTGCATA AAAATATTAT CCAGCATTAT GCTGGCTTAC CAGTTTTTAG TCTATTTAAC CAGCACTTTG ATCGTTATCC CGTTCGTTAT GAAGCGTTTC AACGATTTGG TTCAGATATT TTCTGGAAAA AAACCAAACG GTCATCTTCA TCATGACTAA TATTCAGAAT TCTATCCGGT AAAAAGGATG AACTAGTGAA TACAATAAAA CAGTCAGGAT TAATTGAAAT ATTAGGCATT AACCATATTG AATTGGAAAG CATTTTGTAT GAAAATACGA CTACACGTCT GACAATGGAA CTACCATATA TACTTAACTT ATACCGTCTG GCAAAATTCA TTCAACTTCA ATCCATTGAT TATAAAGGTT AATTATGGAA AGAAAACAGA AAAACTCATT ATTTAATTAT ATTTATTCAT TAATGGATGT AAGAGGTAAA TTTTTATTCT TTTCCATGTT ATTCATTACA TCATTATCAT CGATAATCAT ATCTATTTCA CCATTGATTC TTGCAAAGAT TACAGATTTA CTGTCTGGCT CATTGTCAAA TTTTAGTTAT GAATATCTGG TTTTACTTGC CTGTTTATAC ATGTTTTGCG TTATATCTAA TAAAGCAAGT GTTTTTTTAT TTATGATACT GCAAAGTAGT CTACGTATTA ACATGCAGAA AAAAATGTCG CTAAAGTATT TGAGAGAATT GTATAACGAA AATATAACTA ACTTGAGTAA AAATAATGCT GGATATACAA CGCAAAGTCT TAACCAGGCT TCAAATGACA TTTATATTCT TGTGAGAAAT GTTTCCCAGA ATATCCTGTC ACCTGTTATA CAACTTATTT CCACTATTGT TGTTGTTTTA TCTACGAAGG ACTGGTTTTC TGCCGGTGTG TTTTTTCTCT ATATTCTGGT ATTTGTAATT TTTAATACCA GACTGACTGG CAGTTTAGCG TCTCTCAGAA AACACAGCAT GGATATCACT CTTAACTCTT ATAGTCTGTT ATCTGATACT GTTGATAACA TGATAGCAGC TAAAAAGAAT AATGCATTAA GACTTATTTC TGAACGTTAT GAAGATGCTC TCACTCAGGA AAACAATGCT CAGAAAAAAT ACTGGTTACT CAGTTCTAAA GTTCTTTTAT TGAACTCTTT ACTTGCTGTA ATATTATTTG GTTCTGTATT CATATATAAT ATTTTAGGTG TGCTGAATGG TGTAGTTAGT ATCGGCCACT TCATTATGAT TACATCATAT ATCATTCTTC TTTCAACGCC AGTGGAAAAT ATAGGGGCAT TGCTAAGTGA GATCAGGCAG TCAATGTCTA GCCTGGCAGG TTTTATTCAA CGTCATGCCG AGAATAAAGC CACATCTCCT TCAATACCTT TTCTCAACAT GGAGCGAAAA TTAAACCTGT CCATAAGAGA GCTTTCATTT AGCTATAGTG ATGATAAAAA AATACTTAAT TCAGTCAGTC TTGACCTTTT TACCGGAAAA ATGTATTCAT TAACCGGACC CAGTGGTTCA GGAAAATCCA CCCTTGTAAA AATAATATCA GGTTACTATA AAAATTACTT TGGAGACATT TATCTGAATG ATATATCCTT ACGTAATATC AGTGATGAGG ATTTGAATGA TGCTATTTAC TACCTAACAC AAGATGATTA TATTTTTATG GATACACTAC GATTTAATCT CCGGCTCGCA AATTACGACG CGTCAGAAAA TGAAATATTT AAAGTTCTTA AACTGGCAAA TCTTTCTGTC GTCAACAATG AACCAGTGAG TCTGGATACA CACCTTATAA ACAGAGGCAA TAACTATTCA GGAGGGCAAA AACAACGAAT TTCGTTAGCG CGACTGTTTT TGAGAAAACC TGCAATAATT ATTATTGATG AAGCCACATC GGCTCTGGAT TATATTAATG AATCAGAAAT TTTATCATCA ATAAGAACTC ATTTTCCTGA TGCGTTAATT ATAAATATTA GTCACCGAAT AAATCTTCTG GAGTGTTCCG ATTGTGTTTA TGTATTGAAT GAAGGAAATA TTGTTGCTTC TGGCCATTTC AGGGATTTGA TGGTCAGCAA TGAATACATA TCGGGACTGG CTTCTGTTAC TGAATAAAAG CTTGCGGCCG CATAATGCTT AAGTCGAACA GAAAGTAATC GTATTGTACA CGGCCGCATA ATCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT CCCCATCTTA GTATATTAGT TAAGTATAAG AAGGAGATAT ACATATGACC ATGTACGCAA CGCTTGAAGA AGCCATTGAC GCTGCACGCG AAGAATTTCT TGCAGACAAC CCCGGCATCG ACGCCGAAGA TGCGAATGTG CAACAGTTCA ATGCCCAAAA ATACGTTTTG CAGGACGGCG ACATCATGTG GCAAGTTGAG TTTTTTGCCG ACGAAGGGGA AGAAGGTGAA TGTTTACCTA TGCTTAGCGG TGAAGCCGCG CAAAGTGTTT TTGATGGCGA CTATGATGAG ATAGAGATAC GCCAGGAGTG GCAGGAAGAG AATACATTAC ATGAATGGGA CGAGGGGGAA TTTCAGCTTG AGCCACCGCT GGATACCGAG GAAGGACGCG CAGCAGCTGA TGAGTGGGAT GAACGTATTA AGCATTTTCA TTTTAATAAA CTGTCTTCTG GTAAAAAAAA TAATGTTCCA TCTCCTGCAA AGGGGGTTAT ACAAATAAAA AAATCAGCAT CGCAACTCAC AAAAGGTGGT GCAGGACATG TGCCTGAGTA TTTTGTGGGG ATTGGTACAC CTATATCTTT CTATGGCTGA CTCGAGTCTG GTAAAGAAAC CGCTGCTGCG AAATTTGAAC GCCAGCACAT GGACTCGTCT ACTAGCGCAG CTTAATTAAC CTAGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TTGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTCT GGCGGCACGA TGGCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT CATGATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCTAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATG TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGC
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