pTac-mCherry-R6K
$84.00
In stock
SKU
PL55086
100μg liquid plasmid(Instantly):$105
| name | pTac-mCherry-R6K | another name | apTac-mCherry-R6K |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Tac | Replicon | R6K |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATAAACCG CCCAGTCTAG CTATCGTCAT GTAAGCCCCC TGCAAGCTAC CTCTTTCTCT TTGCGCTTGC GTTTTCCCTT GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTAGCA CCGTTTCTGC GGACTGGCTT TCTACGTGTT CCGCTTCCTT TAGCAGCCCT TGCGCCCTGA TGCTTGCGGC AGCGCGTTTC CGAGAACCGC GCGAACGACA TGGAGCGGCA CGCGGGCGTG GAAAGCCTGG TCGGCTGGAT CCGGCCACGA TGCGTCCGGC GTAGAGGATC TGAAGATCAG CAGTTCAACC TGTTGATAGT ACGTACTAAG CTCTCATGTT TCACGTACTA AGCTCTCATG TTTAACGTAC TAAGCTCTCA TGTTTAACGA ACTAAACCCT CATGGCTAAC GTACTAAGCT CTCATGGCTA ACGTACTAAG CTCTCATGTT TCACGTACTA AGCTCTCATG TTTGAACAAT AAAATTAATA TAAATCAGCA ACTTAAATAG CCTCTAAGGT TTTAAGTTTT ATAAGAAAAA AAAGAATATA TAAGGCTTTT AAAGCTTTTA AGGTTTAACG GTTGTGGACA ACAAGCCAGG GATGTAACGC ACTGAGAAGC CCTTAGAGCC TCTCAAAGCA ATTTTGAGTG ACACAGGAAC ACTTAACGGC TGACATGGGA ATTCCACATG TGGAATTCCA CATGTGGAAT TGTGAGCGGA TAACAATTTG TGGAATTCCC GGGAGAGCTA CTGACTCTTA TACACAAGTT CGGCCACGGC CTAGGCCCAT GGTTGACAAT TAATCATCGG CTCGTATAAT GCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACTCGAGAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCT TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGTAGG AGCTCCAAAT AAAACGAAAA GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATGCGGCCGC AGCTTGTCGA CGAATTCCCG GGGATCCGGT GATTGATTGA GCAAGCTTTA TGCTTGTAAA CCGTTTTGTG AAAAAATTTT TAAAATAAAA AAGGGGACCT CTAGGGTCCC CAATTAATTA GTAATATAAT CTATTAAAGG TCATTCAAAA GGTCATCCAC CGGATCAATT CCCCTGCTCA CACAGACTGG GTACCAAGCT CTCGGTAACA TCAAGGCCCG ATCCTTGGAG CCCTTGCCCT CCCGACGATG ATCGTGCCGT GATCGAAATC CAGATCCTTG ACCCACAGTT GCAAACCCTC ACTGATCCGT CCCGTCCGGG TCGACCAAAG CGGCCATCGT GCCTCCCCAC TCCTGCAGTT CGGGGGCATG GATGCGCGGA TAGCCGCTGC TGGTTTCCTG GATGCCGACG GATTTGCACT GCCGGTAGAA CTCCGCGAGG TCGTCCAGCC TCAGGCAGCA GCTGAACCAA CTCGCGAGGG GATCGAGCCC GGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCG CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGGGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCCTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCAG ATCTTGATCC CCTGCGCCAT CAGATCCTTG GCGGCAAGAA AGCCATCCAG TTTACTTTGC AGGGCTTCCC AACCTTACCA GAGGGCGCCC CAGCTGGCAA TTCCGGTTCG CTTGCTGTCC ATAAAACCGC CCAGTCTAGC TATCGTCATG TAAGCCCACT GCAAGCTACC TCTTTCTCTT TGCGCTTGCG TTTTCCCTTG TCCAGATAGC CCAGAAGCTG ACATTCATCC CGGGGTAGCA CCGGCTTTC
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