PYCm-HSP70-Linker-GFP
$99.00
In stock
SKU
PL54978
100μg liquid plasmid(Instantly):$120
| name | PYCm-HSP70-Linker-GFP | another name | aPYCm-HSP70-Linker-GFP |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCACTATCCA ACTACACTCA TAATGCTTAA AGAAAATTGG CATTATTTTA CCAATTTTTT TAAAACAAAA AAAGAATAAA ATATTTTCTT ATTATTTAGC TAAAATTGTA CTAAATTTTA TACCAAAAAA AATATATATA AATATATGTA ATATATAATA TATATATATA TATATGTATA TATGTATGTA TGTATATATG TATGTATGTA TATATGTATG TATGTATATA TGTATGTATG TATGTATAGT TTATATTTAT TTGGCAAGTT TTTCTGTAAA AATGTTTAAT TAAAAAAAAA AATATATAAC ATGCCAATAT GCACTATTCA TTTTATGGAG GCAATTTTAT GAGTAACCAT TTTTACTTTT TTTTTACATA TGCGCATACT TCGAGTTATA CAATGTTATT GGAGACGCGT AGAGGCCTCG TCTCAGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTCAACA TATGCTGATA ACCAACCAGG AGTCTTAATA CAAGTATATG AAGGAGAAAG AGCTTTAACA AAAGATAATA ATTTATTAGG TAAATTTCAT TTAGATGGTA TCCCACCTGC TCCTAGAAAA GTACCACAAA TTGAAGTTAC ATTCGATATT GATGCTAATG GTATTTTAAA TGTTACAGCT GTTGAAAAAA GTACTGGTAA ACAAAACCAT ATTACAATTA CAAATGATAA AGGTAGATTA TCTCCTGAAG AAATTGATAG AATGGTTAAT GATGCTGAAA AATATAAAGC AGAAGATGAA GAAAATAAAA AAAGAATTGA AGCCAGAAAT AGTCTTGAAA ATTATTGCTA TGGTGTTAAA AGTTCATTAG AAGATCAAAA AATTAAAGAA AAATTACAAC CTAATGAAGT TGAAACATGT ATGAAATCTG TTACTTCTAT TCTTGAATGG TTAGAAAAAA ATCAATTAGC TGGAAAGGAT GAATATGAAG CTAAACAAAA AGAAGCTGAA GCTGTATGCT CTCCAATTAT GTCTAAAATA TATCAAGATG CTGGTGCAGC TGCTGGTGGT ATGCCAGGTG GAATGCCAGG TGGAATGCCA GGAGGAATGC CAGGAGGAAT GCCAGGAGGA ATGAACTTCC CAGGTGGAAT GCCAGGAGGA ATGGGAGCAC CCGCTGGAGC CCCAGCGGGC TCGGGACCAA CTGTTGAAGA AGTTGATGGT TCGGGAATGA GTAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGCCA ACACTTGTCA CTACTTTCGG TTATGGTGTT CAATGCTTTG CGAGATACCC AGATCATATG AAACAGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA CGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG AATGGAATCA AAGTTAACTT CAAAATTAGA CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG ATTACACATG GCATGGATGA ACTATACAAA TAAATAAATT AATTTTTTTT TTTTTTTTTT TTTTATATTG TTGTTCTGTA GTTCTTCTTT TGTGAATAGT ATTTTTATAT ATGTACTGTT AATTTTAAAA TGTGGAACGC CACGAACATA TTTTCTCGAA CAAAATATAT ATTTTTTGTG TCGAAATATA TAAATTTGTG TCGAATATAT AAATTTGTGT ACTTCCTTTT TGTTGTATGC ATGAAAAAAA GGAATAACAT CAAAGGGGGT CGAGTTATTA TGCTCTCTAG ACCCTAAAAC AAACTTGTAC ATCACCACTA CTTTAATCAT ACGTAATATC ACTACTTTAA GCATACGTAA TATCACTACT TTAATCATAC GTAATATCAT TAGATGTAAT ATCATCAGAT GTAATATCAT CAGATGTAAT ATCATCAGAT GTAATATCAT TAAATGTGAA AGCGTGTTAG CACTTGGTAT CGGTTTAGCT TTCCAGTCAT GCTGGCAATC ACATGCTAAT ATGTGAGGTG TGCCCTTTTT AACTACACTC AAAATAACAT GATTTAATTA ACATTGGCGA GCAGCATATC ACTTTTGATG CACTAGCTCT GAAATGTGTA CAAGTTTTCA TTTGCTAAAG TTACAAAAAA AGGGGGAGTT TTTATTTGAG GCAAGTGCGT TAAAATGGAA AATTAAAAAA ATGAAAAAAT GAAAAAAAAT GAAAAATTAA AAAAAATGAA AAAATGAAAG AATGGCAGAA TGGTGAAAGA GCGGGAGGTG GCGAATTCCT TAAGTTTACA ATTTAATATT CATACTTTAA GTATTTTTTG TAGTATCCTA GATATTGTGC TTTAAATGCT CACCCCTCAA AGCACCAGTA ATATTTTCAT CCACTGAAAT ACCATTAAAT TTTCAAAAAA ATACTATGCA TATAATGTTA TACATATAAA CATAAAACGC CATGTAAATC AAAAAATATA TAAAAATATG TATAAAAATA AATATGCACT AAATATAAGC TAATTATGCA TAAAAATTAA AGTGCCCTTT ATTAACTAGT CGTAATTATT TATATTTCTA TGTTATAAAA AAATCCTCAT ATAATAATAT AATTAATATA TGTAATGTTT TTTTTATTTT ATAATTTTAA TATAAAATAA TATGTAAATT AATTCAAAAA ATAAATATAA TTGTTGTGAA ACAAAAAACG TAATTTTTTC ATTTGCCTTC AAAATTTAAA TTTATTTTAA TATTTCCTAA AATATATATA CTTTGTGTAT AAATATATAA AAATATATAT TTGCTTATAA ATAAATAAAA AATTTTATAA AACATAGGGG GATCTATGGT TGGTTCGCTA AACTGCATCG TCGCTGTGTC CCAGAACATG GGCATCGGCA AGAACGGGGA CCTGCCCTGG CCACCGCTCA GGAACGAATT TAGATATTTC CAGAGAATGA CCACAACCTC TTCAGTAGAA GGTAAACAGA ATCTGGTGAT TATGGGTAAG AAGACCTGGT TCTCCATTCC TGAGAAGAAT CGACCTTTAA AGGGTAGAAT TAATTTAGTT CTCAGCAGAG AACTCAAGGA ACCTCCACAA GGAGCTCATT TTCTTTCCAG AAGTCTAGAT GATGCCTTAA AACTTACTGA ACAACCAGAA TTAGCAAATA AAGTAGACAT GGTCTGGATA GTTGGTGGCA GTTCTGTTTA TAAGGAAGCC ATGAATCACC CAGGCCATCT TAAACTATTT GTGACAAGGA TCATGCAAGA CTTTGAAAGT GACACGTTTT TTCCAGAAAT TGATTTGGAG AAATATAAAC TTCTGCCAGA ATACCCAGGT GTTCTCTCTG ATGTCCAGGA GGAGAAAGGC ATTAAGTACA AATTTGAAGT ATATGAGAAG AATGATGCAA GCGGAGGAGG TGGATCTGGT GGAGGTGGAA GTGCAAGCGT GACAGGGGGA ATGGCTAGCA AGTGGGATCA GAAGGGTATG GACATTGCCT ATGAGGAGGC GGCCTTAGGT TACAAAGAGG GTGGTGTTCC TATTGGCGGA TGTCTTATCA ATAACAAAGA CGGAAGTGTT CTCGGTCGTG GTCACAACAT GAGATTTCAA AAGGGATCCG CCACACTACA TGGTGAGATC TCCACTTTGG AAAACTGTGG GAGATTAGAG GGCAAAGTGT ACAAAGATAC CACTTTGTAT ACGACGCTGT CTCCATGCGA CATGTGTACA GGTGCCATCA TCATGTATGG TATTCCACGC TGTGTTGTCG GTGAGAACGT TAATTTCAAA AGTAAGGGCG AGAAATATTT ACAAACTAGA GGTCACGAGG TTGTTGTTGT TGACGATGAG AGGTGTAAAA AGATCATGAA ACAATTTATC GATGAAAGAC CTCAGGATTG GTTTGAAGAT ATTGGTGAGG CTTCGGAACC ATTTAAGAAC GTCTACTTGC TACCTCAAAC AAACCAATTG CTGGGTTTGT ACACCATCAT CAGAAATAAG AATACAACTA GACCTGATTT CATTTTCTAC TCCGATAGAA TCATCAGATT GTTGGTTGAA GAAGGTTTGA ACCATCTACC TGTGCAAAAG CAAATTGTGG AAACTGACAC CAACGAAAAC TTCGAAGGTG TCTCATTCAT GGGTAAAATC TGTGGTGTTT CCATTGTCAG AGCTGGTGAA TCGATGGAGC AAGGATTAAG AGACTGTTGT AGGTCTGTGC GTATCGGTAA AATTTTAATT CAAAGGGACG AGGAGACTGC TTTACCAAAG TTATTCTACG AAAAATTACC AGAGGATATA TCTGAAAGGT ATGTCTTCCT ATTAGACCCA ATGCTGGCCA CCGGTGGTAG TGCTATCATG GCTACAGAAG TCTTGATTAA GAGAGGTGTT AAGCCAGAGA GAATTTACTT CTTAAACCTA ATCTGTAGTA AGGAAGGGAT TGAAAAATAC CATGCCGCCT TCCCAGAGGT CAGAATTGTT ACTGGTGCCC TCGACAGAGG TCTAGATGAA AACAAGTATC TAGTTCCAGG GTTGGGTGAC TTTGGTGACA GATACTACTG TGTTGGTTCG GGAGAGGGCA GAGGATCCCT GCTAACATGC GGTGATGTCG AGGAGAATCC TGGCCCAGAA TCGATGGACT ATAAGGACCA CGACGGAGAC TACAAGGATC ATGATATTGA TTACAAAGAC GATGACGATA AGATGGCCCC AAAGAAGAAG CGGAAGGTCG GTATCCACGG AGTCCCAGCA GCCGACAAGA AGTACAGCAT CGGCCTGGAC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG ATCACCGACG AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG CACAGCATCA AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA AACAGCCGAG GCCACCCGGC TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC TATCTGCAAG AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA CTGGAAGAGT CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC AACATCGTGG ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG AAACTGGTGG ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC CCTGGCCCAC ATGATCAAGT TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC GTGGACAAGC TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA GGAAAACCCC ATCAACGCCA GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG CAAGAGCAGA CGGCTGGAAA ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT GTTCGGAAAC CTGATTGCCC TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA CCTGGCCGAG GATGCCAAAC TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGCG ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC CGACGCCATC CTGCTGAGCG ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT GAGCGCCTCT ATGATCAAGA GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC TCTCGTGCGG CAGCAGCTGC CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA CGGCTACGCC GGCTACATTG ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA GCCCATCCTG GAAAAGATGG ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA CCTGCTGCGG AAGCAGCGGA CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG AGAGCTGCAC GCCATTCTGC GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG GGAAAAGATC GAGAAGATCC TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG GGGAAACAGC AGATTCGCCT GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA CTTCGAGGAA GTGGTGGACA AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA CTTCGATAAG AACCTGCCCA ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA CTTCACCGTG TATAACGAGC TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC CGCCTTCCTG AGCGGCGAGC AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG GAAAGTGACC GTGAAGCAGC TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC CGTGGAAATC TCCGGCGTGG AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT GCTGAAAATT ATCAAGGACA AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA AGATATCGTG CTGACCCTGA CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA AACCTATGCC CACCTGTTCG ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC CGGCTGGGGC AGGCTGAGCC GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA GACAATCCTG GATTTCCTGA AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT CCACGACGAC AGCCTGACCT TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG CGATAGCCTG CACGAGCACA TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT CCTGCAGACA GTGAAGGTGG TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA GAACATCGTG ATCGAAATGG CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG CCGCGAGAGA ATGAAGCGGA TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA AGAACACCCC GTGGAAAACA CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA GAATGGGCGG GATATGTACG TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA TGTGGACCAT ATCGTGCCTC AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT GACCAGAAGC GACAAGAACC GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA GAAGATGAAG AACTACTGGC GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT CGACAATCTG ACCAAGGCCG AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT CAAGAGACAG CTGGTGGAAA CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC CCGGATGAAC ACTAAGTACG ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC CCTGAAGTCC AAGCTGGTGT CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA GATCAACAAC TACCACCACG CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT GATCAAAAAG TACCCTAAGC TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA CGTGCGGAAG ATGATCGCCA AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT CTTCTACAGC AACATCATGA ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT CCGGAAGCGG CCTCTGATCG AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG CCGGGATTTT GCCACCGTGC GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA GACCGAGGTG CAGACAGGCG GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA TAAGCTGATC GCCAGAAAGA AGGACTGGGA CCCTAAGAAG TACGGCGGCT TCGACAGCCC CACCGTGGCC TATTCTGTGC TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT GAAGAGTGTG AAAGAGCTGC TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA TCCCATCGAC TTTCTGGAAG CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA GCTGCCTAAG TACTCCCTGT TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC CGGCGAACTG CAGAAGGGAA ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA CCTGGCCAGC CACTATGAGA AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT GTTTGTGGAA CAGCACAAGC ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC CAAGAGAGTG ATCCTGGCCG ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA CCGGGATAAG CCCATCAGAG AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA TCTGGGAGCC CCTGCCGCCT TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC CAGCACCAAA GAGGTGCTGG ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA GACACGGATC GACCTGTCTC AGCTGGGAGG CGACAAAAGG CCGGCGGCCA CGAAAAAGGC CGGCCAGGCA AAAAAGAAAA AGTAAGAATT ACCGGTGATC CCGTTTTTCT TACTTATATA TTTATACCAA TTGATTGTAT TTATAACTGT AAAAATGTGT ATGTTGTGTG CATATTTTTT TTTGTGCATG CACATGCATG TAAATAGCTA AAATTATGAA CATTTTATTT TTTGTTCAGA AAAAAAAAAC TTTACACACA TAAAATGGCT AGTATGAATA GCCATATTTT ATATAAATTA AATCCTATGA ATTTATGACC ATATTAAAAA TTTAGATATT TATGGAACAT AATATGTTTG AAACAATAAG ACAAAATTAT TATTATTATT ATTATTTTTA CTGTTATAAT TATGTTGTCT CTTCAATGAT TCATAAATAG TTGGACTTGA TTTTTAAAAT GTTTATAATA TGATTAGCAT AGTTAAATAA AAAAAGTTGA AAAATTAAAA AAAAACATAT AAACACAAAT GATGTTTTTT CCTTCAATTT CGG
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