pTX179-AG-sgRNA1-sgRNA2-UBI-Cas9
$99.00
In stock
SKU
PL54500
100μg liquid plasmid(Instantly):$120
| name | pTX179-AG-sgRNA1-sgRNA2-UBI-Cas9 | another name | apTX179-AG-sgRNA1-sgRNA2-UBI-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | UBI | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAGTAGAC GAGAGTGTCG TGCTCCACCA TGGGCGCGCC TGCAGTGCAG CGTGACCCGG TCGTGCCCCT CTCTTGAGAT AAGGTGAGCA TTGCATGTCT AAGTTATAAA AAATTACCAC ATATTTTTTT TGTCACACTT GTTTGAAGTG CAGTTTATCT ATCTTTATAC ATATATTTAA ACTTTACTCT ACGAATAATA TAATCTATAG TACTACAATA ATATCAGTGT TTTAGAGAAT CATATAAATG AACAGTTAGA CATGGTCTAA AGGACAATTG AGTATTTTGA CAACAGGACT CTACAGTTTT ATCTTTTTAG TGTGCATGTG TTCTCCTTTT TTTTTGCAAA TAGCTTCACC TATATAATAC TTCATCCATT TTATTAGTAC ATCCATTTAG GGTTTAGGGT TAATGGTTTT TATAGACTAA TTTTTTTAGT ACATCTATTT TATTCTATTT TAGCCTCTAA ATTAAGAAAA CTAAAACTCT ATTTTAGTTT TTTTATTTAA TAATTTAGAT ATAAAATAGA ATAAAATAAA GTGACTAAAA ATTAAACAAA TACCCTTTAA GAAATTAAAA AAACTAAGGA AACATTTTTC TTGTTTCGAG TAGATAATGC CAGCCTGTTA AACGCCGTCG ACGAGTCTAA CGGACACCAA CCAGCGAACC AGCAGCGTCG CGTCGGGCCA AGCGAAGCAG ACGGCACGGC ATCTCTGTCG CTGCCTCTGG ACCCCTCTCG AGAGTTCCGC TCCACCGTTG GACTTGCTCC GCTGTCGGCA TCCAGAAATT GCGTGGCGGA GCGGCAGACG TGAGCCGGCA CGGCAGGCGG CCTCCTCCTC CTCTCACGGC ACCGGCAGCT ACGGGGGATT CCTTTCCCAC CGCTCCTTCG CTTTCCCTTC CTCGCCCGCC GTAATAAATA GACACCCCCT CCACACCCTC TTTCCCCAAC CTCGTGTTGT TCGGAGCGCA CACACACACA ACCAGAACTC CCCCAAATCC ACCCGTCGGC ACCTCCGCTT CAAGGTACGC CGCTCGTCCT CCCCCCCCCC CTCTCTACCT TCTCAAGATC GGCGTTCCGG TCCATGGTTA GGGCCCGGTA GTTCTACTTC TGTTCATGTT TGTGTTAGAT CCGTGTTTGT GTTAGATCCG TGCTACTAGC GTTCGTACAC GGATGCGACC TGTACGTCAG ACACGTTCTG ATTGCTAACT TGCCAGTGTT TCTCTTTGGG GAATCCTGGG ATGGCTCTAG CCGTTCCGCA GACGGGATCG ATTTCATGAT TTTTTTTGTT TCGTTGCATA GGGTTTGGTT TGCCCTTTTC CTTTATTTCA ATATATGCCG TGCACTTGTT TGTCGGGTCA TCTTTTCATG CTTTTTTTTG TCTTGGTTGT GATGATGTGG TCTGGTTGGG CGGTCGTTCA AGATCGGAGT AGAATTAATT CTGTTTCAAA CTACCTGGTG GATTTATTAA TTTTGGATCT GTATGTGTGT GCCATACATA TTCATAGTTA CGAATTGAAG ATGATGGATG GAAATATCGA TCTAGGATAG GTATACATGT TGATGCGGGT TTTACTGATG CATATACAGA GATGCTTTTT GTTCGCTTGG TTGTGATGAT GTGGTGTGGT TGGGCGGTCG TTCATTCGTT CAAGATCGGA GTAGAATACT GTTTCAAACT ACCTGGTGTA TTTATTAATT TTGGAACTGT ATGTGTGTGT CATACATCTT CATAGTTACG AGTTTAAGAT GGATGGAAAT ATCGATCTAG GATAGGTATA CATGTTGATG TGGGTTTTAC TGATGCATAT ACATGATGGC ATATGCAGCA TCTATTCATA TGCTCTAACC TTGAGTACCT ATCTATTATA ATAAACAAGT ATGTTTTATA ATTATTTTGA TCTTGATATA CTTGGATGAT GGCATATGCA GCAGCTATAT GTGGATTTTT TTAGCCCTGC CTTCATACGC TATTTATTTG CTTGGTACTG TTTCTTTTGT CGATGCTCAC CCTGTTGTTT GGTGTTACTT CTGCAGCCTG CAGGTAGATC GCTCGTCGAC ATGGATAAGA AGTACTCTAT CGGACTCGCT ATCGGAACTA ACTCTGTGGG ATGGGCTGTG ATCACCGATG AGTACAAGGT GCCATCTAAG AAGTTCAAGG TTCTCGGAAA CACCGATAGG CACTCTATCA AGAAAAACCT TATCGGTGCT CTCCTCTTCG ATTCTGGTGA AACTGCTGAG GCTACCAGAC TCAAGAGAAC CGCTAGAAGA AGGTACACCA GAAGAAAGAA CAGGATCTGC TACCTCCAAG AGATCTTCTC TAACGAGATG GCTAAAGTGG ATGATTCATT CTTCCACAGG CTCGAAGAGT CATTCCTCGT GGAAGAAGAT AAGAAGCACG AGAGGCACCC TATCTTCGGA AACATCGTTG ATGAGGTGGC ATACCACGAG AAGTACCCTA CTATCTACCA CCTCAGAAAG AAGCTCGTTG ATTCTACTGA TAAGGCTGAT CTCAGGCTCA TCTACCTCGC TCTCGCTCAC ATGATCAAGT TCAGAGGACA CTTCCTCATC GAGGGTGATC TCAACCCTGA TAACTCTGAT GTGGATAAGT TGTTCATCCA GCTCGTGCAG ACCTACAACC AGCTTTTCGA AGAGAACCCT ATCAACGCTT CAGGTGTGGA TGCTAAGGCT ATCCTCTCTG CTAGGCTCTC TAAGTCAAGA AGGCTTGAGA ACCTCATTGC TCAGCTCCCT GGTGAGAAGA AGAACGGACT TTTCGGAAAC TTGATCGCTC TCTCTCTCGG ACTCACCCCT AACTTCAAGT CTAACTTCGA TCTCGCTGAG GATGCAAAGC TCCAGCTCTC AAAGGATACC TACGATGATG ATCTCGATAA CCTCCTCGCT CAGATCGGAG ATCAGTACGC TGATTTGTTC CTCGCTGCTA AGAACCTCTC TGATGCTATC CTCCTCAGTG ATATCCTCAG AGTGAACACC GAGATCACCA AGGCTCCACT CTCAGCTTCT ATGATCAAGA GATACGATGA GCACCACCAG GATCTCACAC TTCTCAAGGC TCTTGTTAGA CAGCAGCTCC CAGAGAAGTA CAAAGAGATT TTCTTCGATC AGTCTAAGAA CGGATACGCT GGTTACATCG ATGGTGGTGC ATCTCAAGAA GAGTTCTACA AGTTCATCAA GCCTATCCTC GAGAAGATGG ATGGAACCGA GGAACTCCTC GTGAAGCTCA ATAGAGAGGA TCTTCTCAGA AAGCAGAGGA CCTTCGATAA CGGATCTATC CCTCATCAGA TCCACCTCGG AGAGTTGCAC GCTATCCTTA GAAGGCAAGA GGATTTCTAC CCATTCCTCA AGGATAACAG GGAAAAGATT GAGAAGATTC TCACCTTCAG AATCCCTTAC TACGTGGGAC CTCTCGCTAG AGGAAACTCA AGATTCGCTT GGATGACCAG AAAGTCTGAG GAAACCATCA CCCCTTGGAA CTTCGAAGAG GTGGTGGATA AGGGTGCTAG TGCTCAGTCT TTCATCGAGA GGATGACCAA CTTCGATAAG AACCTTCCAA ACGAGAAGGT GCTCCCTAAG CACTCTTTGC TCTACGAGTA CTTCACCGTG TACAACGAGT TGACCAAGGT TAAGTACGTG ACCGAGGGAA TGAGGAAGCC TGCTTTTTTG TCAGGTGAGC AAAAGAAGGC TATCGTTGAT CTCTTGTTCA AGACCAACAG AAAGGTGACC GTGAAGCAGC TCAAAGAGGA TTACTTCAAG AAAATCGAGT GCTTCGATTC AGTTGAGATT TCTGGTGTTG AGGATAGGTT CAACGCATCT CTCGGAACCT ACCACGATCT CCTCAAGATC ATTAAGGATA AGGATTTCTT GGATAACGAG GAAAACGAGG ATATCTTGGA GGATATCGTT CTTACCCTCA CCCTCTTTGA AGATAGAGAG ATGATTGAAG AAAGGCTCAA GACCTACGCT CATCTCTTCG ATGATAAGGT GATGAAGCAG TTGAAGAGAA GAAGATACAC TGGTTGGGGA AGGCTCTCAA GAAAGCTCAT TAACGGAATC AGGGATAAGC AGTCTGGAAA GACAATCCTT GATTTCCTCA AGTCTGATGG ATTCGCTAAC AGAAACTTCA TGCAGCTCAT CCACGATGAT TCTCTCACCT TTAAAGAGGA TATCCAGAAG GCTCAGGTTT CAGGACAGGG TGATAGTCTC CATGAGCATA TCGCTAACCT CGCTGGATCT CCTGCAATCA AGAAGGGAAT CCTCCAGACT GTGAAGGTTG TGGATGAGTT GGTGAAGGTG ATGGGAAGGC ATAAGCCTGA GAACATCGTG ATCGAAATGG CTAGAGAGAA CCAGACCACT CAGAAGGGAC AGAAGAACTC TAGGGAAAGG ATGAAGAGGA TCGAGGAAGG TATCAAAGAG CTTGGATCTC AGATCCTCAA AGAGCACCCT GTTGAGAACA CTCAGCTCCA GAATGAGAAG CTCTACCTCT ACTACCTCCA GAACGGAAGG GATATGTATG TGGATCAAGA GTTGGATATC AACAGGCTCT CTGATTACGA TGTTGATCAT ATCGTGCCAC AGTCATTCTT GAAGGATGAT TCTATCGATA ACAAGGTGCT CACCAGGTCT GATAAGAACA GGGGTAAGAG TGATAACGTG CCAAGTGAAG AGGTTGTGAA GAAAATGAAG AACTATTGGA GGCAGCTCCT CAACGCTAAG CTCATCACTC AGAGAAAGTT CGATAACTTG ACTAAGGCTG AGAGGGGAGG ACTCTCTGAA TTGGATAAGG CAGGATTCAT CAAGAGGCAG CTTGTGGAAA CCAGGCAGAT CACTAAGCAC GTTGCACAGA TCCTCGATTC TAGGATGAAC ACCAAGTACG ATGAGAACGA TAAGTTGATC AGGGAAGTGA AGGTTATCAC CCTCAAGTCA AAGCTCGTGT CTGATTTCAG AAAGGATTTC CAATTCTACA AGGTGAGGGA AATCAACAAC TACCACCACG CTCACGATGC TTACCTTAAC GCTGTTGTTG GAACCGCTCT CATCAAGAAG TATCCTAAGC TCGAGTCAGA GTTCGTGTAC GGTGATTACA AGGTGTACGA TGTGAGGAAG ATGATCGCTA AGTCTGAGCA AGAGATCGGA AAGGCTACCG CTAAGTATTT CTTCTACTCT AACATCATGA ATTTCTTCAA GACCGAGATT ACCCTCGCTA ACGGTGAGAT CAGAAAGAGG CCACTCATCG AGACAAACGG TGAAACAGGT GAGATCGTGT GGGATAAGGG AAGGGATTTC GCTACCGTTA GAAAGGTGCT CTCTATGCCA CAGGTGAACA TCGTTAAGAA AACCGAGGTG CAGACCGGTG GATTCTCTAA AGAGTCTATC CTCCCTAAGA GGAACTCTGA TAAGCTCATT GCTAGGAAGA AGGATTGGGA CCCTAAGAAA TACGGTGGTT TCGATTCTCC TACCGTGGCT TACTCTGTTC TCGTTGTGGC TAAGGTTGAG AAGGGAAAGA GTAAGAAGCT CAAGTCTGTT AAGGAACTTC TCGGAATCAC TATCATGGAA AGGTCATCTT TCGAGAAGAA CCCAATCGAT TTCCTCGAGG CTAAGGGATA CAAAGAGGTT AAGAAGGATC TCATCATCAA GCTCCCAAAG TACTCACTCT TCGAACTCGA GAACGGTAGA AAGAGGATGC TCGCTTCTGC TGGTGAGCTT CAAAAGGGAA ACGAGCTTGC TCTCCCATCT AAGTACGTTA ACTTTCTTTA CCTCGCTTCT CACTACGAGA AGTTGAAGGG ATCTCCAGAA GATAACGAGC AGAAGCAACT TTTCGTTGAG CAGCACAAGC ACTACTTGGA TGAGATCATC GAGCAGATCT CTGAGTTCTC TAAAAGGGTG ATCCTCGCTG ATGCAAACCT CGATAAGGTG TTGTCTGCTT ACAACAAGCA CAGAGATAAG CCTATCAGGG AACAGGCAGA GAACATCATC CATCTCTTCA CCCTTACCAA CCTCGGTGCT CCTGCTGCTT TCAAGTACTT CGATACAACC ATCGATAGGA AGAGATACAC CTCTACCAAA GAAGTGCTCG ATGCTACCCT CATCCATCAG TCTATCACTG GACTCTACGA GACTAGGATC GATCTCTCAC AGCTCGGTGG TGATTCAAGG GCTGATCCTA AGAAGAAGAG GAAGGTTTGA GGATCTAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAGGCT TTTGTCACCG GACAATTCTA ACACCGGATC GGGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTGTCACCGG AATTAATTTC TGCCACCGAT CGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TGTCACCGGA TGTGTTTTCC GGTCTGATGA GTCCGTGAGG ACGAAACAAA AGCGAGCTCA AACTATCAGT GTTTGACAGG ATATATTGGC GGGTAAACCT AAGAGAAAAG AGCGTTTATT AGAATAATCG GATATTTAAA AGGGCGTGAA AAGGTTTATC CGTTCGTCCA TTTGTATGTG CATGCCAACC ACAGGGTTCC CCTCGGGATC AAAGTACTTT GATCCAACCC CTCCGCTGCT ATAGTGCAGT CGGCTTCTGA CGTTCAGTGC AGCCGTCTTC TGAAAACGAC ATGTCGCACA AGTCCTAAGT TACGCGACAG GCTGCCGCCC TGCCCTTTTC CTGGCGTTTT CTTGTCGCGT GTTTTAGTCG CATAAAGTAG AATACTTGCG ACTAGAACCG GAGACATTAC GCCATGAACA AGAGCGCCGC CGCTGGCCTG CTGGGCTATG CCCGCGTCAG CACCGACGAC CAGGACTTGA CCAACCAACG GGCCGAACTG CACGCGGCCG GCTGCACCAA GCTGTTTTCC GAGAAGATCA CCGGCACCAG GCGCGACCGC CCGGAGCTGG CCAGGATGCT TGACCACCTA CGCCCTGGCG ACGTTGTGAC AGTGACCAGG CTAGACCGCC TGGCCCGCAG CACCCGCGAC CTACTGGACA TTGCCGAGCG CATCCAGGAG GCCGGCGCGG GCCTGCGTAG CCTGGCAGAG CCGTGGGCCG ACACCACCAC GCCGGCCGGC CGCATGGTGT TGACCGTGTT CGCCGGCATT GCCGAGTTCG AGCGTTCCCT AATCATCGAC CGCACCCGGA GCGGGCGCGA GGCCGCCAAG GCCCGAGGCG TGAAGTTTGG CCCCCGCCCT ACCCTCACCC CGGCACAGAT CGCGCACGCC CGCGAGCTGA TCGACCAGGA AGGCCGCACC GTGAAAGAGG CGGCTGCACT GCTTGGCGTG CATCGCTCGA CCCTGTACCG CGCACTTGAG CGCAGCGAGG AAGTGACGCC CACCGAGGCC AGGCGGCGCG GTGCCTTCCG TGAGGACGCA TTGACCGAGG CCGACGCCCT GGCGGCCGCC GAGAATGAAC GCCAAGAGGA ACAAGCATGA AACCGCACCA GGACGGCCAG GACGAACCGT TTTTCATTAC CGAAGAGATC GAGGCGGAGA TGATCGCGGC CGGGTACGTG TTCGAGCCGC CCGCGCACGG CTCAACCGTG CGGCTGCATG AAATCCTGGC CGGTTTGTCT GATGCCAAGC TGGCGGCCTG GCCGGCCAGC TTGGCCGCTG AAGAAACCGA GCGCCGCCGT CTAAAAAGGT GATGTGTATT TGAGTAAAAC AGCTTGCGTC ATGCGGTCGC TGCGTATATG ATGCGATGAG TAAATAAACA AATACGCAAG GGGAACGCAT GAAGGTTATC GCTGTACTTA ACCAGAAAGG CGGGTCAGGC AAGACGACCA TCGCAACCCA TCTAGCCCGC GCCCTGCAAC TCGCCGGGGC CGATGTTCTG TTAGTCGATT CCGATCCCCA GGGCAGTGCC CGCGATTGGG CGGCCGTGCG GGAAGATCAA CCGCTAACCG TTGTCGGCAT CGACCGCCCG ACGATTGACC GCGACGTGAA GGCCATCGGC CGGCGCGACT TCGTAGTGAT CGACGGAGCG CCCCAGGCGG CGGACTTGGC TGTGTCCGCG ATCAAGGCAG CCGACTTCGT GCTGATTCCG GTGCAGCCAA GCCCTTACGA CATATGGGCC ACCGCCGACC TGGTGGAGCT GGTTAAGCAG CGCATTGAGG TCACGGATGG AAGGCTACAA GCGGCCTTTG TCGTGTCGCG GGCGATCAAA GGCACGCGCA TCGGCGGTGA GGTTGCCGAG GCGCTGGCCG GGTACGAGCT GCCCATTCTT GAGTCCCGTA TCACGCAGCG CGTGAGCTAC CCAGGCACTG CCGCCGCCGG CACAACCGTT CTTGAATCAG AACCCGAGGG CGACGCTGCC CGCGAGGTCC AGGCGCTGGC CGCTGAAATT AAATCAAAAC TCATTTGAGT TAATGAGGTA AAGAGAAAAT GAGCAAAAGC ACAAACACGC TAAGTGCCGG CCGTCCGAGC GCACGCAGCA GCAAGGCTGC AACGTTGGCC AGCCTGGCAG ACACGCCAGC CATGAAGCGG GTCAACTTTC AGTTGCCGGC GGAGGATCAC ACCAAGCTGA AGATGTACGC GGTACGCCAA GGCAAGACCA TTACCGAGCT GCTATCTGAA TACATCGCGC AGCTACCAGA GTAAATGAGC AAATGAATAA ATGAGTAGAT GAATTTTAGC GGCTAAAGGA GGCGGCATGG AAAATCAAGA ACAACCAGGC ACCGACGCCG TGGAATGCCC CATGTGTGGA GGAACGGGCG GTTGGCCAGG CGTAAGCGGC TGGGTTGCCT GCCGGCCCTG CAATGGCACT GGAACCCCCA AGCCCGAGGA ATCGGCGTGA GCGGTCGCAA ACCATCCGGC CCGGTACAAA TCGGCGCGGC GCTGGGTGAT GACCTGGTGG AGAAGTTGAA GGCCGCGCAG GCCGCCCAGC GGCAACGCAT CGAGGCAGAA GCACGCCCCG GTGAATCGTG GCAAGCGGCC GCTGATCGAA TCCGCAAAGA ATCCCGGCAA CCGCCGGCAG CCGGTGCGCC GTCGATTAGG AAGCCGCCCA AGGGCGACGA GCAACCAGAT TTTTTCGTTC CGATGCTCTA TGACGTGGGC ACCCGCGATA GTCGCAGCAT CATGGACGTG GCCGTTTTCC GTCTGTCGAA GCGTGACCGA CGAGCTGGCG AGGTGATCCG CTACGAGCTT CCAGACGGGC ACGTAGAGGT TTCCGCAGGG CCGGCCGGCA TGGCCAGTGT GTGGGATTAC GACCTGGTAC TGATGGCGGT TTCCCATCTA ACCGAATCCA TGAACCGATA CCGGGAAGGG AAGGGAGACA AGCCCGGCCG CGTGTTCCGT CCACACGTTG CGGACGTACT CAAGTTCTGC CGGCGAGCCG ATGGCGGAAA GCAGAAAGAC GACCTGGTAG AAACCTGCAT TCGGTTAAAC ACCACGCACG TTGCCATGCA GCGTACGAAG AAGGCCAAGA ACGGCCGCCT GGTGACGGTA TCCGAGGGTG AAGCCTTGAT TAGCCGCTAC AAGATCGTAA AGAGCGAAAC CGGGCGGCCG GAGTACATCG AGATCGAGCT AGCTGATTGG ATGTACCGCG AGATCACAGA AGGCAAGAAC CCGGACGTGC TGACGGTTCA CCCCGATTAC TTTTTGATCG ATCCCGGCAT CGGCCGTTTT CTCTACCGCC TGGCACGCCG CGCCGCAGGC AAGGCAGAAG CCAGATGGTT GTTCAAGACG ATCTACGAAC GCAGTGGCAG CGCCGGAGAG TTCAAGAAGT TCTGTTTCAC CGTGCGCAAG CTGATCGGGT CAAATGACCT GCCGGAGTAC GATTTGAAGG AGGAGGCGGG GCAGGCTGGC CCGATCCTAG TCATGCGCTA CCGCAACCTG ATCGAGGGCG AAGCATCCGC CGGTTCCTAA TGTACGGAGC AGATGCTAGG GCAAATTGCC CTAGCAGGGG AAAAAGGTCG AAAAGGCCTC TTTCCTGTGG ATAGCACGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGAACCCG TACATTGGGA ACCCAAAGCC GTACATTGGG AACCGGTCAC ACATGTAAGT GACTGATATA AAAGAGAAAA AAGGCGATTT TTCCGCCTAA AACTCTTTAA AACTTATTAA AACTCTTAAA ACCCGCCTGG CCTGTGCATA ACTGTCTGGC CAGCGCACAG CCGAAGAGCT GCAAAAAGCG CCTACCCTTC GGTCGCTGCG CTCCCTACGC CCCGCCGCTT CGCGTCGGCC TATCGCGGCC GCTGGCCGCT CAAAAATGGC TGGCCTACGG CCAGGCAATC TACCAGGGCG CGGACAAGCC GCGCCGTCGC CACTCGACCG CCGGCGCCCA CATCAAGGCA CCCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAA ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGCATTCTA GGTACTAAAA CAATTCATCC AGTAAAATAT AATATTTTAT TTTCTCCCAA TCAGGCTTGA TCCCCAGTAA GTCAAAAAAT AGCTCGACAT ACTGTTCTTC CCCGATATCC TCCCTGATCG ACCGGACGCA GAAGGCAATG TCATACCACT TGTCCGCCCT GCCGCTTCTC CCAAGATCAA TAAAGCCACT TACTTTGCCA TCTTTCACAA AGATGTTGCT GTCTCCCAGG TCGCCGTGGG AAAAGACAAG TTCCTCTTCG GGCTTTTCCG TCTTTAAAAA ATCATACAGC TCGCGCGGAT CTTTAAATGG AGTGTCTTCT TCCCAGTTTT CGCAATCCAC ATCGGCCAGA TCGTTATTCA GTAAGTAATC CAATTCGGCT AAGCGGCTGT CTAAGCTATT CGTATAGGGA CAATCCGATA TGTCGATGGA GTGAAAGAGC CTGATGCACT CCGCATACAG CTCGATAATC TTTTCAGGGC TTTGTTCATC TTCATACTCT TCCGAGCAAA GGACGCCATC GGCCTCACTC ATGAGCAGAT TGCTCCAGCC ATCATGCCGT TCAAAGTGCA GGACCTTTGG AACAGGCAGC TTTCCTTCCA GCCATAGCAT CATGTCCTTT TCCCGTTCCA CATCATAGGT GGTCCCTTTA TACCGGCTGT CCGTCATTTT TAAATATAGG TTTTCATTTT CTCCCACCAG CTTATATACC TTAGCAGGAG ACATTCCTTC CGTATCTTTT ACGCAGCGGT ATTTTTCGAT CAGTTTTTTC AATTCCGGTG ATATTCTCAT TTTAGCCATT TATTATTTCC TTCCTCTTTT CTACAGTATT TAAAGATACC CCAAGAAGCT AATTATAACA AGACGAACTC CAATTCACTG TTCCTTGCAT TCTAAAACCT TAAATACCAG AAAACAGCTT TTTCAAAGTT GTTTTCAAAG TTGGCGTATA ACATAGTATC GACGGAGCCG ATTTTGAAAC CGCGGTGATC ACAGGCAGCA ACGCTCTGTC ATCGTTACAA TCAACATGCT ACCCTCCGCG AGATCATCCG TGTTTCAAAC CCGGCAGCTT AGTTGCCGTT CTTCCGAATA GCATCGGTAA CATGAGCAAA GTCTGCCGCC TTACAACGGC TCTCCCGCTG ACGCCGTCCC GGACTGATGG GCTGCCTGTA TCGAGTGGTG ATTTTGTGCC GAGCTGCCGG TCGGGGAGCT GTTGGCTGGC TGGTGGCAGG ATATATTGTG GTGTAAACAA ATTGACGCTT AGACAACTTA ATAACACATT GCGGACGTTT TTAATGTACT GAATTAACGC CGAATTAATT CGGGGGATCT GGATTTTAGT ACTGGATTTT GGTTTTAGGA ATTAGAAATT TTATTGATAG AAGTATTTTA CAAATACAAA TACATACTAA GGGTTTCTTA TATGCTCAAC ACATGAGCGA AACCCTATAG GAACCCTAAT TCCCTTATCT GGGAACTACT CACACATTAT TATGGAGAAA ATAGAGCTTG TCGATCGTTA ATTAATCACC TCCAAGATCC CGGACGAGTG CTGGGGCGTC GGTTTCCACT ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG GAGCATATAC GCCCGGAGCC GCGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCACG AGGTGCCGGA CTTCGGGGCA GTCCTCGGCC CAAAGCATCA GCTCATCGAG AGCCTGCGCG ACGGACGCAC TGACGGTGTC GTCCATCACA GTTTGCCAGT GATACACATG GGGATCAGCA ATCGCGCATA TGAAATCACG CCATGTAGTG TATTGACCGA TTCCTTGCGG TCCGAATGGG CCGAACCCGC TCGTCTGGCT AAGATCGGCC GCAGCGATCG CATCCATGGC CTCCGCGACC GGCTGCAGAA CAGCGGGCAG TTCGGTTTCA GGCAGGTCTT GCAACGTGAC ACCCTGTGCA CGGCGGGAGA TGCAATAGGT CAGGCTCTCG CTGAATTCCC CAATGTCAAG CACTTCCGGA ATCGGGAGCG CGGCCGATGC AAAGTGCCGA TAAACATAAC GATCTTTGTA GAAACCATCG GCGCAGCTAT TTACCCGCAG GACATATCCA CGCCCTCCTA CATCGAAGCT GAAAGCACGA GATTCTTCGC CCTCCGAGAG CTGCATCAGG TCGGACACGC TGTCGAACTT TTCGATCAGA AACTTCTCGA CAGACGTCGC GGTGAGTTCA GGCTTTTTCA TCCTAGGAGA GATAGATTTG TAGAGAGAGA CTGGTGATTT CAGCGTGTCC TCTCCAAATG AAATGAACTT CCTTATATAG AGGAAGGGTC TTGCGAAGGA TAGTGGGATT GTGCGTCATC CCTTACGTCA GTGGAGATAT CACATCAATC CACTTGCTTT GAAGACGTGG TTGGAACGTC TTCTTTTTCC ACGATGCTCC TCGTGGGTGG GGGTCCATCT TTGGGACCAC TGTCGGCAGA GGCATCTTGA ACGATAGCCT TTCCTTTATC GCAATGATGG CATTTGTAGG TGCCACCTTC CTTTTCTACT GTCCTTTTGA TGAAGTGACA GATAGCTGGG CAATGGAATC CGAGGAGGTT TCCCGATATT ACCCTTTGTT GAAAAGTCTC AATAGCCCTT TGGTCTTCTG AGACTGTATC TTTGATATTC TTGGAGTAGA CGAGAGTGTC GTGCTCCACC ATGTTCACAT CAATCCACTT GCTTTGAAGA CGTGGTTGGA ACGTCTTCTT TTTCCACGAT GCTCCTCGTG GGTGGGGGTC CATCTTTGGG ACCACTGTCG GCAGAGGCAT CTTGAACGAT AGCCTTTCCT TTATCGCAAT GATGGCATTT GTAGGTGCCA CCTTCCTTTT CTACTGTCCT TTTGATGAAG TGACAGATAG CTGGGCAATG GAATCCGAGG AGGTTTCCCG ATATTACCCT TTGTTGAAAA GTCTCAATAG CCCTTTGGTC TTCTGAGACT GTATCTTTGA TATTCT
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