pTargetF-poxB(E.coli)-J23119-sgRNA1
$99.00
In stock
SKU
PL54486
100μg liquid plasmid(Instantly):$142
| name | pTargetF-poxB(E.coli)-J23119-sgRNA1 | another name | apTargetF-poxB(E.coli)-J23119-sgRNA1 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Escherichia coli | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | J23119 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGAGTTCAT GTGCAGCTCC ATAAGCAAAA GGGGATGATA AGTTTATCAC CACCGACTAT TTGCAACAGT GCCGTTGATC GTGCTATGAT CGACTGATGT CATCAGCGGT GGAGTGCAAT GTCATGAGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT AGTTGGCGTC ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC CGCAGTGGAT GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA CGGTGACCGT AAGGCTTGAT GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC CCCTGGAGAG AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT CATTCCGTGG CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA CATTCTTGCA GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC AAAAGCAAGA GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC GGTTCCTGAA CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC GCCCGACTGG GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG CGCAGTAACC GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT GCCGGCCCAG TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA AGATCGCTTG GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG TGAAAGGCGA GATCACCAAG GTAGTCGGCA AATAAGATGC CGCTCGCCAG TCGATTGGCT GAGCTCATGA AGTTCCTATT CCGAAGTTCC GCGAACGCGT AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGGC TTTGTGGCGA TGGAGATGAA AGCTGGTGGC TATTTGACTG ACGGCACCGA ACTACACGAC ACAAACTTTG CCCGCATTGC CGAAGCGTGC GGCATTACGG GTATCCGTGT AGAAAAAGCG TCTGAAGTTG ATGAAGCCCT GCAACGCGCC TTCTCCATCG ACGGTCCGGT GTTGGTGGAT GTGGTGGTCG CCAAAGAAGA GTTAGCCATT CCACCGCAGA TCAAACTCGA ACAGGCCAAA GGTTTCAGCC TGTATATGCT GCGCGCAATC ATCAGCGGAC GCGGTGATGA AGTGATCGAA CTGGCGAAAA CAAACTGGCT AAGGTAAAAA GGGTGGCATT TCCCGTCATA ATAAGGACAT GCCATGATTG ATTTACGCAG TGATACCGTT ACCCGACCAA GCCGCGCCAT GCTCGAAGCG ATGATGGCCG CCCCGGTTGG GGACGACGTT TACGGAGACG ACCCTACCGT TAATGCTCCT GACTTAGCTT CACGTACCGT GATGACCTGC GGCCCGGCTC CGTATATGGA TTGGGTAGAG CAGGAAGTGA AAGCGCTCGG CGTGACGCGT TTCTTTAAAG AGAAATTCTT CACCCCAGTA GCAGAAGCGG CGACCAGCGG TCTGAAATTC ACCAAACTGC AACCGGCACG AGAATTTTAC GCCCCGGTTG GCACCACGCT ACTGGAGGCG CTGGAAAGCA ATAACGTTCC GGTTGTCGCT GCCTGCCGTG CGGGTGTTTG CGGCTGCTGT AAGACAAAAG TGGTTTCCGG TGAATATACG GTGAGCAGCA CAATGACGCT GACCGACGCC GAAATCGCTG AAGGTTACGT ACTGGCCTGC TCCTGCCATC CGCAGGGGGA TTTGGTTCTC GCATAATCGC CTTATGCCCG ATGATATTCC TTTCATCGGG CTATTTAACC GTTAGTGCCT CCTTTCTCTC CCATCCCTTC CCCCTCCGTC AGATGAACTA AACTTGTTAC CGTTATCACA TTCAGGAGAT GGAGAACCAT GAAACAAACG GTTGCAGCTT ATATCGCCAA AACACTCGAA TCGGCAGGGG TGAAACGCAT CTGGGGAGGA TCCTTGACAG CTAGCTCAGT CCTAGGTATA ATACTAGTGC AGGGGTGAAA CGCATCTGGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT TGAATTCTCT AGAGTCGACC TGCAGAAGCT TAGATCTATT ACCCTGTTAT CCCTAC
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