pKI1.1R-AtU6-AG-sgRNA1-RPS5A-Cas9
$84.00
In stock
SKU
PL54258
100μg liquid plasmid(Instantly):$105
| name | pKI1.1R-AtU6-AG-sgRNA1-RPS5A-Cas9 | another name | apKI1.1R-AtU6-AG-sgRNA1-RPS5A-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Spe | |
| Selective marker | Hyg | Fluorescent labeling | Red |
| Promoter | AtU6-26 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGGTCATAA CGTGACTCCC TTAATTCTCC GCTCATGATC GATAATTCGC GGTACCCGGG GATCCTCTAG AGGGCCCGAC GTCGCATGCT CCCGGCCGCC ATGGCCGCGG GATATCACAA GTTTGTACAA AAAAGCAGGC TCCGCGGCCG CTCGTTGAAC AACGGAAACT CGACTTGCCT TCCGCACAAT ACATCATTTC TTCTTAGCTT TTTTTCTTCT TCTTCGTTCA TACAGTTTTT TTTTGTTTAT CAGCTTACAT TTTCTTGAAC CGTAGCTTTC GTTTTCTTCT TTTTAACTTT CCATTCGGAG TTTTTGTATC TTGTTTCATA GTTTGTCCCA GGATTAGAAT GATTAGGCAT CGAACCTTCA AGAATTTGAT TGAATAAAAC ATCTTCATTC TTAAGATATG AAGATAATCT TCAAAAGGCC CCTGGGAATC TGAAAGAAGA GAAGCAGGCC CATTTATATG GGAAAGAACA ATAGTATTTC TTATATAGGC CCATTTAAGT TGAAAACAAT CTTCAAAAGT CCCACATCGC TTAGATAAGA AAACGAAGCT GAGTTTATAT ACAGCTAGAG TCGAAGTAGT GATTGGGACA ATTCTAACAC CGGATGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTTT TTTGCGGCCG CGATCCCTCA ACTTTTGATT CGCTATTTGC AGTGCACCTG TGGCGTTCAT CACATCTTTT GTGACACTGT TTGCACTGGT CATTGCTATT ACAAAGGACC TTCCTGATGT TGAAGGAGAT CGAAAGTAAG TAACTGCACG CATAACCATT TTCTTTCCGC TCTTTGGCTC AATCCATTTG ACAGTCAAAG ACAATGTTTA ACCAGCTCCG TTTGATATAT TGTCTTTATG TGTTTGTTCA AGCATGTTTA GTTAATCATG CCTTTGATTG ATCTTGAATA GGTTCCAAAT ATCAACCCTG GCAACAAAAC TTGGAGTGAG AAACATTGCA TTCCTCGGTT CTGGACTTCT GCTAGTAAAT TATGTTTCAG CCATATCACT AGCTTTCTAC ATGCCTCAGG TGAATTCATC TATTTCCGTT TTAACTATTT CGGTTAATTA AAGCACGAAC ACCATTACTG CATGTAGAAG CTTGATAAAC TATCGCCACC AATTTATTTT TGTTGCGATA TTGTTACTTT CCTCAGTATG CAGCTTTGAA AAGACCAACC CTCTTATCCT TTAACAATGA ACAGGTTTTT AGAGGTAGCT TGATGATTCC TGCACATGTG ATCTTGGCTT CAGGCTTAAT TTTCCAGGTA AAGCATTATG AGATACTCTT ATATCTCTTA CATACTTTTG AGATAATGCA CAAGAACTTC ATAACTATAT GCTTTAGTTT CTGCATTTGA CACTGCCAAA TTCATTAATC TCTAATATCT TTGTTGTTGA TCTTTGGTAG ACATGGGTAC TAGAAAAAGC AAACTACACC AAGGTAAAAT ACTTTTGTAC AAACATAAAC TCGTTATCAC GGAACATCAA TGGAGTGTAT ATCTAACGGA GTGTAGAAAC ATTTGATTAT TGCAGGAAGC TATCTCAGGA TATTATCGGT TTATATGGAA TCTCTTCTAC GCAGAGTATC TGTTATTCCC CTTCCTCTAG CTTTCAATTT CATGGTGAGG ATATGCAGTT TTCTTTGTAT ATCATTCTTC TTCTTCTTTG TAGCTTGGAG TCAAAATCGG TTCCTTCATG TACATACATC AAGGATATGT CCTTCTGAAT TTTTATATCT TGCAATAAAA ATGCTTGTAC CAATTGAAAC ACCAGCTTTT TGAGTTCTAT GATCACTGAC TTGGTTCTAA CCAAAAAAAA AAAATGTTTA ATTTACATAT CTAAAAGTAG GTTTAGGGAA ACCTAAACAG TAAAATATTT GTATATTATT CGAATTTCAC TCATCATAAA AACTTAAATT GCACCATAAA ATTTTGTTTT ACTATTAATG ATGTAATTTG TGTAACTTAA GATAAAAATA ATATTCCGTA AGTTAACCGG CTAAAACCAC GTATAAACCA GGGAACCTGT TAAACCGGTT CTTTACTGGA TAAAGAAATG AAAGCCCATG TAGACAGCTC CATTAGAGCC CAAACCCTAA ATTTCTCATC TATATAAAAG GAGTGACATT AGGGTTTTTG TTCGTCCTCT TAAAGCTTCT CGTTTTCTCT GCCGTCTCTC TCATTCGCGC GACGCAAACG ATCTTCAGGT GATCTTCTTT CTCCAAATCC TCTCTCATAA CTCTGATTTC GTACTTGTGT ATTTGAGCTC ACGCTCTGTT TCTCTCACCA CAGCCCCATG GACTATAAGG ACCACGACGG AGACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC CGACCTGCGG CTGATCTATC TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT GATCGAGGGC GACCTGAACC CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC AACCAGCTGT TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT GCCCGGCGAG AAGAAGAATG GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC CCCCAACTTC AAGAGCAACT TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA CACCTACGAC GACGACCTGG ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT GTTTCTGGCC GCCAAGAACC TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA CACCGAGATC ACCAAGGCCC CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA CCAGGACCTG ACCCTGCTGA AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA GATTTTCTTC GACCAGAGCA AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA GGAAGAGTTC TACAAGTTCA TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT GCTCGTGAAG CTGAACAGAG AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG CATCCCCCAC CAGATCCACC TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT TTACCCATTC CTGAAGGACA ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC CTACTACGTG GGCCCTCTGG CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG CGAGGAAACC ATCACCCCCT GGAACTTCGA GGAAGTGGTG GACAAGGGCG CTTCCGCCCA GAGCTTCATC GAGCGGATGA CCAACTTCGA TAAGAACCTG CCCAACGAGA AGGTGCTGCC CAAGCACAGC CTGCTGTACG AGTACTTCAC CGTGTATAAC GAGCTGACCA AAGTGAAATA CGTGACCGAG GGAATGAGAA AGCCCGCCTT CCTGAGCGGC GAGCAGAAAA AGGCCATCGT GGACCTGCTG TTCAAGACCA ACCGGAAAGT GACCGTGAAG CAGCTGAAAG AGGACTACTT CAAGAAAATC GAGTGCTTCG ACTCCGTGGA AATCTCCGGC GTGGAAGATC GGTTCAACGC CTCCCTGGGC ACATACCACG ATCTGCTGAA AATTATCAAG GACAAGGACT TCCTGGACAA TGAGGAAAAC GAGGACATTC TGGAAGATAT CGTGCTGACC CTGACACTGT TTGAGGACAG AGAGATGATC GAGGAACGGC TGAAAACCTA TGCCCACCTG TTCGACGACA AAGTGATGAA GCAGCTGAAG CGGCGGAGAT ACACCGGCTG GGGCAGGCTG AGCCGGAAGC TGATCAACGG CATCCGGGAC AAGCAGTCCG GCAAGACAAT CCTGGATTTC CTGAAGTCCG ACGGCTTCGC CAACAGAAAC TTCATGCAGC TGATCCACGA CGACAGCCTG ACCTTTAAAG AGGACATCCA GAAAGCCCAG GTGTCCGGCC AGGGCGATAG CCTGCACGAG CACATTGCCA ATCTGGCCGG CAGCCCCGCC ATTAAGAAGG GCATCCTGCA GACAGTGAAG GTGGTGGACG AGCTCGTGAA AGTGATGGGC CGGCACAAGC CCGAGAACAT CGTGATCGAA ATGGCCAGAG AGAACCAGAC CACCCAGAAG GGACAGAAGA ACAGCCGCGA GAGAATGAAG CGGATCGAAG AGGGCATCAA AGAGCTGGGC AGCCAGATCC TGAAAGAACA CCCCGTGGAA AACACCCAGC TGCAGAACGA GAAGCTGTAC CTGTACTACC TGCAGAATGG GCGGGATATG TACGTGGACC AGGAACTGGA CATCAACCGG CTGTCCGACT ACGATGTGGA CCATATCGTG CCTCAGAGCT TTCTGAAGGA CGACTCCATC GACAACAAGG TGCTGACCAG AAGCGACAAG AACCGGGGCA AGAGCGACAA CGTGCCCTCC GAAGAGGTCG TGAAGAAGAT GAAGAACTAC TGGCGGCAGC TGCTGAACGC CAAGCTGATT ACCCAGAGAA AGTTCGACAA TCTGACCAAG GCCGAGAGAG GCGGCCTGAG CGAACTGGAT AAGGCCGGCT TCATCAAGAG ACAGCTGGTG GAAACCCGGC AGATCACAAA GCACGTGGCA CAGATCCTGG ACTCCCGGAT GAACACTAAG TACGACGAGA ATGACAAGCT GATCCGGGAA GTGAAAGTGA TCACCCTGAA GTCCAAGCTG GTGTCCGATT TCCGGAAGGA TTTCCAGTTT TACAAAGTGC GCGAGATCAA CAACTACCAC CACGCCCACG ACGCCTACCT GAACGCCGTC GTGGGAACCG CCCTGATCAA AAAGTACCCT AAGCTGGAAA GCGAGTTCGT GTACGGCGAC TACAAGGTGT ACGACGTGCG GAAGATGATC GCCAAGAGCG AGCAGGAAAT CGGCAAGGCT ACCGCCAAGT ACTTCTTCTA CAGCAACATC ATGAACTTTT TCAAGACCGA GATTACCCTG GCCAACGGCG AGATCCGGAA GCGGCCTCTG ATCGAGACAA ACGGCGAAAC CGGGGAGATC GTGTGGGATA AGGGCCGGGA TTTTGCCACC GTGCGGAAAG TGCTGAGCAT GCCCCAAGTG AATATCGTGA AAAAGACCGA GGTGCAGACA GGCGGCTTCA GCAAAGAGTC TATCCTGCCC AAGAGGAACA GCGATAAGCT GATCGCCAGA AAGAAGGACT GGGACCCTAA GAAGTACGGC GGCTTCGACA GCCCCACCGT GGCCTATTCT GTGCTGGTGG TGGCCAAAGT GGAAAAGGGC AAGTCCAAGA AACTGAAGAG TGTGAAAGAG CTGCTGGGGA TCACCATCAT GGAAAGAAGC AGCTTCGAGA AGAATCCCAT CGACTTTCTG GAAGCCAAGG GCTACAAAGA AGTGAAAAAG GACCTGATCA TCAAGCTGCC TAAGTACTCC CTGTTCGAGC TGGAAAACGG CCGGAAGAGA ATGCTGGCCT CTGCCGGCGA ACTGCAGAAG GGAAACGAAC TGGCCCTGCC CTCCAAATAT GTGAACTTCC TGTACCTGGC CAGCCACTAT GAGAAGCTGA AGGGCTCCCC CGAGGATAAT GAGCAGAAAC AGCTGTTTGT GGAACAGCAC AAGCACTACC TGGACGAGAT CATCGAGCAG ATCAGCGAGT TCTCCAAGAG AGTGATCCTG GCCGACGCTA ATCTGGACAA AGTGCTGTCC GCCTACAACA AGCACCGGGA TAAGCCCATC AGAGAGCAGG CCGAGAATAT CATCCACCTG TTTACCCTGA CCAATCTGGG AGCCCCTGCC GCCTTCAAGT ACTTTGACAC CACCATCGAC CGGAAGAGGT ACACCAGCAC CAAAGAGGTG CTGGACGCCA CCCTGATCCA CCAGAGCATC ACCGGCCTGT ACGAGACACG GATCGACCTG TCTCAGCTGG GAGGCGACAA AAGGCCGGCG GCCACGAAAA AGGCCGGCCA GGCAAAAAAG AAAAAGTAAA AGGGTGGGAT ATGAAGATGA AGATGAAATA TTTGGTGTGT CAAATAAAAA GCTTGTGTGC TTAAGTTTGT GTTTTTTTCT TGGCTTGTTG TGTTATGAAT TTGTGGCTTT TTCTAATATT AAATGAATGT AAGATCTCAT TATAATGAAT AAACAAATGT TTCTATAATC CATTGTGAAT GTTTTGTTGG ATCTCTTCTG CAGCATATAA CTACTGTATG TGCTATGGTA TGGACTATGG AATATGATTA AAGATAAGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TGATATCACT AGTGTATGTA GGTATAGTAA CATGAACAAG AACAGACTCA AGTACAAGAT CGCATACGAA AATGGAAATG GCAATGTCAC TTCCACATAA TCAAACACGA ATCCTCATAT CAACAAGGCC TGAGATTCTA ACTAGCTCAT AACAACTTAG CCAATAGTTA CTTGAGACTA CCAAATGTAT GTAGAACTAA AGACTAAGGG ACAGAGAGTT CGTCTAAACA GGTGAATCTA GTCGTTGTTA TCTAATAAAC AATTCAGCCC CAAATGCAGA ACACACATAG AGCTCTCTAT TGATTCAAAT TACGATCTGA TACTGATAAC GTCTAGATTT TTAGGGTTAA AGCAATCAAT CACCTGACGA TTCAAGGTGG TTGGATCATG ACGATTCCAG AAAACATCAA GCAAGCTCTC AAAGCTACAC TCTTTGGGAT CATACTGAAC TCTAACAACC TCGTTATGTC CCGTAGTGCC AGTACAGACA TCCTCGTAAC TCGGATTGTG CACGATGCCA TGGCTATACC CAACCTCGGT CTTGGTCACA CCAGGAACTC TCTGGTAAGC TAGCTCCACT CCCCAGAAAC AACCGGCGCC AAATTGCGCG AATTGCTGAC CTGAAGACGG AACATCATCG TCGGGTCCTT GGGCGATTGC GGCGGAAGAT GGGTCAGCTT GGGCTTGAGG ACGAGACCCG AATCCGAGTC TGTTGAAAAG GTTGTTCATT GGGGATTTGT ATACGGAGAT TGGTCGTCGA GAGGTTTGAG GGAAAGGACA AATGGGTTTG GCTCTGGAGA AAGAGAGTGC GGCTTTAGAG AGAGAATTGA GAGGTTTAGA GAGAGATGCG GCGGCGATGA GCGGAGGAGA GACGACGAGG ACCTGCATTA TCAAAGCAGT GACGTGGTGA AATTTGGAAC TTTTAAGAGG CAGATAGATT TATTATTTGT ATCCATTTTC TTCATTGTTC TAGAATGTCG CGGAACAAAT TTTAAAACTA AATCCTAAAT TTTTCTAATT TTGTTGCCAA TAGTGGATAT GTGGGCCGTA TAGAAGGAAT CTATTGAAGG CCCAAACCCA TACTGACGAG CCCAAAGGTT CGTTTTGCGT TTTATGTTTC GGTTCGATGC CAACGCCACA TTCTGAGCTA GGCAAAAAAC AAACGTGTCT TTGAATAGAC TCCTCTCGTT AACACATGCA GCGGCTGCAT GGTGACGCCA TTAACACGTG GCCTACAATT GCATGATGTC TCCATTGACA CGTGACTTCT CGTCTCCTTT CTTAATATAT CTAACAAACA CTCCTACCTC TTCCAAAATA TATACACATC TTTTTGATCA ATCTCTCATT CAAAATCTCA TTCTCTCTAG TAAACAAGAA CAAAAAAATG GCGGATACAG CTAGAGGAAC CCATCACGAT ATCATCGGCA GAGACCAGTA CCCGATGATG GGCCGAGACC GAGACCAGTA CCAGATGTCC GGACGAGGAT CTGACTACTC CAAGTCTAGG CAGATTGCTA AAGCTGCAAC TGCTGTCACA GCTGGTGGTT CCCTCCTTGT TCTCTCCAGC CTTACCCTTG TTGGAACTGT CATAGCTTTG ACTGTTGCAA CACCTCTGCT CGTTATCTTC AGCCCAATCC TTGTCCCGGC TCTCATCACA GTTGCACTCC TCATCACCGG TTTTCTTTCC TCTGGAGGGT TTGGCATTGC CGCTATAACC GTTTTCTCTT GGATTTACAA GTAAGCACAC ATTTATCATC TTACTTCATA ATTTTGTGCA ATATGTGCAT GCATGTGTTG AGCCAGTAGC TTTGGATCAA TTTTTTTGGT CGAATAACAA ATGTAACAAT AAGAAATTGC AAATTCTAGG GAACATTTGG TTAACTAAAT ACGAAATTTG ACCTAGCTAG CTTGAATGTG TCTGTGTATA TCATCTATAT AGGTAAAATG CTTGGTATGA TACCTATTGA TTGTGAATAG GTACGCAACG GGAGAGCACC CACAGGGATC AGACAAGTTG GACAGTGCAA GGATGAAGTT GGGAAGCAAA GCTCAGGATC TGAAAGACAG AGCTCAGTAC TACGGACAGC AACATACTGG TGGGGAACAT GACCGTGACC GTACTCGTGG TGGCCAGCAC ACTACTATGA GCGAGCTGAT TAAGGAGAAC ATGCACATGA AGCTGTACAT GGAGGGCACC GTGAACAACC ACCACTTCAA GTGCACATCC GAGGGCGAAG GCAAGCCCTA CGAGGGCACC CAGACCATGA GAATCAAGGT GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT CGACATCCTG GCTACCAGCT TCATGTACGG CAGCAGAACC TTCATCAACC ACACCCAGGG CATCCCCGAC TTCTTTAAGC AGTCCTTCCC TGAGGGCTTC ACATGGGAGA GAGTCACCAC ATACGAAGAC GGGGGCGTGC TGACCGCTAC CCAGGACACC AGCCTCCAGG ACGGCTGCCT CATCTACAAC GTCAAGATCA GAGGGGTGAA CTTCCCATCC AACGGCCCTG TGATGCAGAA GAAAACACTC GGCTGGGAGG CCAACACCGA GATGCTGTAC CCCGCTGACG GCGGCCTGGA AGGCAGAAGC GACATGGCCC TGAAGCTCGT GGGCGGGGGC CACCTGATCT GCAACTTCAA GACCACATAC AGATCCAAGA AACCCGCTAA GAACCTCAAG ATGCCCGGCG TCTACTATGT GGACCACAGA CTGGAAAGAA TCAAGGAGGC CGACAAAGAG ACCTACGTCG AGCAGCACGA GGTGGCTGTG GCCAGATACT GCGACCTCCC TAGCAAACTG GGGCACAAGT GAATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCACTAGT GCGGCCGCCT GCAGGTCGAC CATATGGGAG AGCTCAAGCT TAGCTTGAGC TTGGATCAGA TTGTCGTTTC CCGCCTTCAG TTTAAACTAT CAGTGTTTGA CAGGATATAT TGGCGGGTAA ACCTAAGAGA AAAGAGCGTT TATTAGAATA ATCGGATATT TAAAAGGGCG TGAAAAGGTT TATCCGTTCG TCCATTTGTA TGTGCATGCC AACCACAGGG TTCCCCTCGG GATCAAAGTA CTTTAAAGTA CTTTAAAGTA CTTTAAAGTA CTTTGATCCA ACCCCTCCGC TGCTATAGTG CAGTCGGCTT CTGACGTTCA GTGCAGCCGT CTTCTGAAAA CGACATGTCG CACAAGTCCT AAGTTACGCG ACAGGCTGCC GCCCTGCCCT TTTCCTGGCG TTTTCTTGTC GCGTGTTTTA GTCGCATAAA GTAGAATACT TGCGACTAGA ACCGGAGACA TTACGCCATG AACAAGAGCG CCGCCGCTGG CCTGCTGGGC TATGCCCGCG TCAGCACCGA CGACCAGGAC TTGACCAACC AACGGGCCGA ACTGCACGCG GCCGGCTGCA CCAAGCTGTT TTCCGAGAAG ATCACCGGCA CCAGGCGCGA CCGCCCGGAG CTGGCCAGGA TGCTTGACCA CCTACGCCCT GGCGACGTTG TGACAGTGAC CAGGCTAGAC CGCCTGGCCC GCAGCACCCG CGACCTACTG GACATTGCCG AGCGCATCCA GGAGGCCGGC GCGGGCCTGC GTAGCCTGGC AGAGCCGTGG GCCGACACCA CCACGCCGGC CGGCCGCATG GTGTTGACCG TGTTCGCCGG CATTGCCGAG TTCGAGCGTT CCCTAATCAT CGACCGCACC CGGAGCGGGC GCGAGGCCGC CAAGGCCCGA GGCGTGAAGT TTGGCCCCCG CCCTACCCTC ACCCCGGCAC AGATCGCGCA CGCCCGCGAG CTGATCGACC AGGAAGGCCG CACCGTGAAA GAGGCGGCTG CACTGCTTGG CGTGCATCGC TCGACCCTGT ACCGCGCACT TGAGCGCAGC GAGGAAGTGA CGCCCACCGA GGCCAGGCGG CGCGGTGCCT TCCGTGAGGA CGCATTGACC GAGGCCGACG CCCTGGCGGC CGCCGAGAAT GAACGCCAAG AGGAACAAGC ATGAAACCGC ACCAGGACGG CCAGGACGAA CCGTTTTTCA TTACCGAAGA GATCGAGGCG GAGATGATCG CGGCCGGGTA CGTGTTCGAG CCGCCCGCGC ACGTCTCAAC CGTGCGGCTG CATGAAATCC TGGCCGGTTT GTCTGATGCC AAGCTGGCGG CCTGGCCGGC CAGCTTGGCC GCTGAAGAAA CCGAGCGCCG CCGTCTAAAA AGGTGATGTG TATTTGAGTA AAACAGCTTG CGTCATGCGG TCGCTGCGTA TATGATGCGA TGAGTAAATA AACAAATACG CAAGGGGAAC GCATGAAGGT TATCGCTGTA CTTAACCAGA AAGGCGGGTC AGGCAAGACG ACCATCGCAA CCCATCTAGC CCGCGCCCTG CAACTCGCCG GGGCCGATGT TCTGTTAGTC GATTCCGATC CCCAGGGCAG TGCCCGCGAT TGGGCGGCCG TGCGGGAAGA TCAACCGCTA ACCGTTGTCG GCATCGACCG CCCGACGATT GACCGCGACG TGAAGGCCAT CGGCCGGCGC GACTTCGTAG TGATCGACGG AGCGCCCCAG GCGGCGGACT TGGCTGTGTC CGCGATCAAG GCAGCCGACT TCGTGCTGAT TCCGGTGCAG CCAAGCCCTT ACGACATATG GGCCACCGCC GACCTGGTGG AGCTGGTTAA GCAGCGCATT GAGGTCACGG ATGGAAGGCT ACAAGCGGCC TTTGTCGTGT CGCGGGCGAT CAAAGGCACG CGCATCGGCG GTGAGGTTGC CGAGGCGCTG GCCGGGTACG AGCTGCCCAT TCTTGAGTCC CGTATCACGC AGCGCGTGAG CTACCCAGGC ACTGCCGCCG CCGGCACAAC CGTTCTTGAA TCAGAACCCG AGGGCGACGC TGCCCGCGAG GTCCAGGCGC TGGCCGCTGA AATTAAATCA AAACTCATTT GAGTTAATGA GGTAAAGAGA AAATGAGCAA AAGCACAAAC ACGCTAAGTG CCGGCCGTCC GAGCGCACGC AGCAGCAAGG CTGCAACGTT GGCCAGCCTG GCAGACACGC CAGCCATGAA GCGGGTCAAC TTTCAGTTGC CGGCGGAGGA TCACACCAAG CTGAAGATGT ACGCGGTACG CCAAGGCAAG ACCATTACCG AGCTGCTATC TGAATACATC GCGCAGCTAC CAGAGTAAAT GAGCAAATGA ATAAATGAGT AGATGAATTT TAGCGGCTAA AGGAGGCGGC ATGGAAAATC AAGAACAACC AGGCACCGAC GCCGTGGAAT GCCCCATGTG TGGAGGAACG GGCGGTTGGC CAGGCGTAAG CGGCTGGGTT GTCTGCCGGC CCTGCAATGG CACTGGAACC CCCAAGCCCG AGGAATCGGC GTGAGCGGTC GCAAACCATC CGGCCCGGTA CAAATCGGCG CGGCGCTGGG TGATGACCTG GTGGAGAAGT TGAAGGCCGC GCAGGCCGCC CAGCGGCAAC GCATCGAGGC AGAAGCACGC CCCGGTGAAT CGTGGCAAGC GGCCGCTGAT CGAATCCGCA AAGAATCCCG GCAACCGCCG GCAGCCGGTG CGCCGTCGAT TAGGAAGCCG CCCAAGGGCG ACGAGCAACC AGATTTTTTC GTTCCGATGC TCTATGACGT GGGCACCCGC GATAGTCGCA GCATCATGGA CGTGGCCGTT TTCCGTCTGT CGAAGCGTGA CCGACGAGCT GGCGAGGTGA TCCGCTACGA GCTTCCAGAC GGGCACGTAG AGGTTTCCGC AGGGCCGGCC GGCATGGCCA GTGTGTGGGA TTACGACCTG GTACTGATGG CGGTTTCCCA TCTAACCGAA TCCATGAACC GATACCGGGA AGGGAAGGGA GACAAGCCCG GCCGCGTGTT CCGTCCACAC GTTGCGGACG TACTCAAGTT CTGCCGGCGA GCCGATGGCG GAAAGCAGAA AGACGACCTG GTAGAAACCT GCATTCGGTT AAACACCACG CACGTTGCCA TGCAGCGTAC GAAGAAGGCC AAGAACGGCC GCCTGGTGAC GGTATCCGAG GGTGAAGCCT TGATTAGCCG CTACAAGATC GTAAAGAGCG AAACCGGGCG GCCGGAGTAC ATCGAGATCG AGCTAGCTGA TTGGATGTAC CGCGAGATCA CAGAAGGCAA GAACCCGGAC GTGCTGACGG TTCACCCCGA TTACTTTTTG ATCGATCCCG GCATCGGCCG TTTTCTCTAC CGCCTGGCAC GCCGCGCCGC AGGCAAGGCA GAAGCCAGAT GGTTGTTCAA GACGATCTAC GAACGCAGTG GCAGCGCCGG AGAGTTCAAG AAGTTCTGTT TCACCGTGCG CAAGCTGATC GGGTCAAATG ACCTGCCGGA GTACGATTTG AAGGAGGAGG CGGGGCAGGC TGGCCCGATC CTAGTCATGC GCTACCGCAA CCTGATCGAG GGCGAAGCAT CCGCCGGTTC CTAATGTACG GAGCAGATGC TAGGGCAAAT TGCCCTAGCA GGGGAAAAAG GTCGAAAAGG TCTCTTTCCT GTGGATAGCA CGTACATTGG GAACCCAAAG CCGTACATTG GGAACCGGAA CCCGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG TCACACATGT AAGTGACTGA TATAAAAGAG AAAAAAGGCG ATTTTTCCGC CTAAAACTCT TTAAAACTTA TTAAAACTCT TAAAACCCGC CTGGCCTGTG CATAACTGTC TGGCCAGCGC ACAGCCGAAG AGCTGCAAAA AGCGCCTACC CTTCGGTCGC TGCGCTCCCT ACGCCCCGCC GCTTCGCGTC GGCCTATCGC GGCCGCTGGC CGCTCAAAAA TGGCTGGCCT ACGGCCAGGC AATCTACCAG GGCGCGGACA AGCCGCGCCG TCGCCACTCG ACCGCCGGCG CCCACATCAA GGCACCCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGCAT GATATATCTC CCAATTTGTG TAGGGCTTAT TATGCACGCT TAAAAATAAT AAAAGCAGAC TTGACCTGAT AGTTTGGCTG TGAGCAATTA TGTGCTTAGT GCATCTAACG CTTGAGTTAA GCCGCGCCGC GAAGCGGCGT CGGCTTGAAC GAATTTCTAG CTAGACATTA TTTGCCGACT ACCTTGGTGA TCTCGCCTTT CACGTAGTGG ACAAATTCTT CCAACTGATC TGCGCGCGAG GCCAAGCGAT CTTCTTCTTG TCCAAGATAA GCCTGTCTAG CTTCAAGTAT GACGGGCTGA TACTGGGCCG GCAGGCGCTC CATTGCCCAG TCGGCAGCGA CATCCTTCGG CGCGATTTTG CCGGTTACTG CGCTGTACCA AATGCGGGAC AACGTAAGCA CTACATTTCG CTCATCGCCA GCCCAGTCGG GCGGCGAGTT CCATAGCGTT AAGGTTTCAT TTAGCGCCTC AAATAGATCC TGTTCAGGAA CCGGATCAAA GAGTTCCTCC GCCGCTGGAC CTACCAAGGC AACGCTATGT TCTCTTGCTT TTGTCAGCAA GATAGCCAGA TCAATGTCGA TCGTGGCTGG CTCGAAGATA CCTGCAAGAA TGTCATTGCG CTGCCATTCT CCAAATTGCA GTTCGCGCTT AGCTGGATAA CGCCACGGAA TGATGTCGTC GTGCACAACA ATGGTGACTT CTACAGCGCG GAGAATCTCG CTCTCTCCAG GGGAAGCCGA AGTTTCCAAA AGGTCGTTGA TCAAAGCTCG CCGCGTTGTT TCATCAAGCC TTACGGTCAC CGTAACCAGC AAATCAATAT CACTGTGTGG CTTCAGGCCG CCATCCACTG CGGAGCCGTA CAAATGTACG GCCAGCAACG TCGGTTCGAG ATGGCGCTCG ATGACGCCAA CTACCTCTGA TAGTTGAGTC GATACTTCGG CGATCACCGC TTCCCCCATG ATGTTTAACT TTGTTTTAGG GCGACTGCCC TGCTGCGTAA CATCGTTGCT GCTCCATAAC ATCAAACATC GACCCACGGC GTAACGCGCT TGCTGCTTGG ATGCCCGAGG CATAGACTGT ACCCCAAAAA AACAGTCATA ACAAGCCATG AAAACCGCCA CTGCGCCGTT ACCACCGCTG CGTTCGGTCA AGGTTCTGGA CCAGTTGCGT GAGCGCATAC GCTACTTGCA TTACAGCTTA CGAACCGAAC AGGCTTATGT CCACTGGGTT CGTGCCCGAA TTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGAAT TAACGCCGAA TTGAATTATC AGCTTGCATG CCGGTCGATC TAGTAACATA GATGACACCG CGCGCGATAA TTTATCCTAG TTTGCGCGCT ATATTTTGTT TTCTATCGCG TATTAAATGT ATAATTGCGG GACTCTAATC ATAAAAACCC ATCTCATAAA TAACGTCATG CATTACATGT TAATTATTAC ATGCTTAACG TAATTCAACA GAAATTATAT GATAATCATC GCAAGACCGG CAACAGGATT CAATCTTAAG AAACTTTATT GCCAAATGTT TGAACGATCT GCTTGACTCT AGGGAATTAA TTCCTGAATC ACTGCGACCG GCCCTCCCGC GACCCAGCCG AGCGAGCTTA GCGAACTGTG GACGAGAACT GTGCCACCAA GCGTAAGGCC GTTCTCTCGC ATTTGCCTTG CTAGGCTCGC GCGAGTTGCT GGCTGAGGCG TTCTCGAAAT CAGCTCTTGT TCGGTCGGCA TCTACTCTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CCCTGCGCCC AAGCTGCATC ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCAATGC GGAGCATATA CGCCCGGAGC CTTGGCGATC CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG CTGCTCCATA CAAGCCAACC ACGGCCTCCA GAAGAAGATG TTGGCGACCT CGTATTGGGA ATCCCCGAAC ATCGCCTCGC TCCAGTCAAT GACCGCTGTT ATGCGGCCAT TGTCCGTCAG GACATTGTTG GAGCCGAAAT CCGCGTGCAC GAGGTGCCGG ACTTCGGGGC AGTCCTCGGC CCAAAGCATC AGCTCATCGA GAGCCTGCGC GACGGACGCA CTGACGGTGT CGTCCATCAC AGTTTGCCAG TGATACACAT GGGGATCAGC AATCGCGCAT ATGAAATCAC GCCATGTAGT GTATTGACCG ATTCCTTGCG GTCCGAATGG GCCGAACCCG CTCGTCTGGC TAAGATCGGC CGCAGCGATA GCATCCATAG CCTCCGCGAC CGGCTGAAGA ACAGCGGGCA GTTCGGTTTC AGGCAGGTCT TGCAACGTGA CACCCTGTGC ACGGCGGGAG ATGCAATAGG TCAGGCTCTC GCTGAACTCC CCAATGTCAA GCACTTCCGG AATCGGGAGC GCGGCCGATG CAAAGTGCCG ATAAACATAA CGATCTTTGT AGAAACCATC GGCGCAGCTA TTTACCCGCA GGACATATCC ACGCCCTCCT ACATCGAAGC TGAAAGCACG AGATTCTTCG CCCTCCGAGA GCTGCATCAG GTCGGAGACG CTGTCGAACT TTTCGATCAG AAACTTCTCG ACAGACGTCG CGGTGAGTTC AGGCTTTTTC ATAGGGGGGA TTCGAGTTGA GAGTGAATAT GAGACTCTAA TTGGATACCG AGGGGAATTT ATGGAACGTC AGTGGAGCAT TTTTGACAAG AAATATTTGC TAGCTGATAG TGACCTTAGG CGACTTTTGA ACGCGCAATA ATGGTTTCTG ACGTATGTGC TTAGCTCATT AAACTCCAGA AACCCGCGGC TCAGTGGCTC CTTCAACGTT GCGGTTCTGT CAGTTCCAAA CGTAAAACGG CTTGTCCCGC GTCATCGGCG
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