pLV2-TRE3GS-APP(human)-K595N-M596L-FLAG-TetOne-Puro
$99.00
In stock
SKU
PL54151
100μg liquid plasmid(Instantly):$120
| name | pLV2-TRE3GS-APP(human)-K595N-M596L-FLAG-TetOne-Puro | another name | apLV2-TRE3GS-APP(human)-K595N-M596L-FLAG-TetOne-Puro |
| Insert Size (bp) | 2085bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACTGCAGAA TTAATTCCAG GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC AACGGGCCAC AACTCCTCAT AAAGAGACAG CAACCAGGAT TTATACAAGG AGGAGAAAAT GAAAGCCATA CGGGAAGCAA TAGCATGATA CAAAGGCATT AAAGCAGCGT ATCCACATAG CGTAAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT TGTAATCCAG AGGTTGATTG TTCCAGACGC GTTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG ATGTCGAGCC CGACGCGCGT GAGGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG CGGTCCGGGT CGACGGTGTG GCGCGTGGCG GGGTAGTCGG CGAACGCGGC GGCGAGGGTG CGTACGGCCC GGGGGACGTC GTCGCGGGTG GCGAGGCGCA CCGTGGGCTT GTACTCGGTC ATGGTCGCGT TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCGCGCGT TTACCCGGGG AGCATGTCAA GGTCAAAATC GTCAAGAGCG TCAGCAGGCA GCATATCAAG GTCAAAGTCG TCAAGGGCAT CGGCTGGGAG CATGTCTAAG TCAAAATCGT CAAGGGCGTC GGTCGGCCCG CCGCTTTCGC ACTTTAGCTG TTTCTCCAGG CCACATATGA TTAGTTCCAG GCCGAAAAGG AAGGCAGGTT CGGCTCCCTG CCGGTCGAAC AGCTCAATTG CTTGTTTCAG AAGTGGGGGC ATAGAATCGG TGGTAGGTGT CTCTCTTTCC TCTTTTGCTA CTTGATGCTC CTGTTCCTCC AATACGCAGC CCAGTGTAAA GTGGCCCACG GCGGACAGAG CGTACAGTGC GTTCTCCAGG GAGAAGCCTT GCTGACACAG GAACGCGAGC TGATTTTCCA GGGTTTCGTA CTGTTTCTCT GTTGGGCGGG TGCCGAGATG CACTTTAGCC CCGTCGCGAT GTGAGAGGAG AGCACAGCGG TATGACTTGG CGTTGTTCCG CAGAAAGTCT TGCCATGACT CGCCTTCCAG GGGGCAGGAG TGGGTATGAT GCCTGTCCAG CATCTCGATT GGCAGGGCAT CGAGCAGGGC CCGCTTGTTC TTCACGTGCC AGTACAGGGT AGGCTGCTCA ACTCCCAGCT TTTGAGCGAG TTTCCTTGTC GTCAGGCCTT CGATACCGAC TCCATTGAGT AATTCCAGAG CAGAGTTTAT GACTTTGCTC TTGTCCAGTC TAGACATGGT AATTCGATGA TCCCCCTGGG GAGAGAGGTC GGTGATTCGG TCAACGAGGG AGCCGACTGC CGACGTGCGC TCCGGAGGCT TGCAGAATGC GGAACACCGC GCGGGCAGGA ACAGGGCCCA CACTACCGCC CCACACCCCG CCTCCCGCAC CGCCCCTTCC CGGCCGCTGC TCTCGGCGCG CCCTGCTGAG CAGCCGCTAT TGGCCACAGC CCATCGCGGT CGGCGCGCTG CCATTGCTCC CTGGCGCTGT CCGTCTGCGA GGGTACTAGT GAGACGTGCG GCTTCCGTTT GTCACGTCCG GCACGCCGCG AACCGCAAGG AACCTTCCCG ACTTAGGGGC GGAGCAGGAA GCGTCGCCGG GGGGCCCACA AGGGTAGCGG CGAAGATCCG GGTGACGCTG CGAACGGACG TGAAGAATGT GCGAGACCCA GGGTCGGCGC CGCTGCGTTT CCCGGAACCA CGCCCAGAGC AGCCGCGTCC CTGCGCAAAC CCAGGGCTGC CTTGGAAAAG GCGCAACCCC AACCCCGTGG AATTATCACC TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGAAGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGCAGACTTT ACTCCCTATC AGTGATAGAG AACGTATAAG GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGACCAGT TTACTCCCTA TCAGTGATAG AGAACGTATC TACAGTTTAC TCCCTATCAG TGATAGAGAA CGTATATCCA GTTTACTCCC TATCAGTGAT AGAGAACGTA TAAGCTTTGC TTATGTAAAC CAGGGCGCCT ATAAAAGAGT GCTGATTTTT TGAGTAAACT TCAATTCCAC AACACTTTTG TCTTATACCA ACTTTCCGTA CCACTTCCTA CCCTCGTAAA GAATTCTTCC GTATACACCG GTGCCACCAT GCTGCCCGGT TTGGCACTGC TCCTGCTGGC CGCCTGGACG GCTCGGGCGC TGGAGGTACC CACTGATGGT AATGCTGGCC TGCTGGCTGA ACCCCAGATT GCCATGTTCT GTGGCAGACT GAACATGCAC ATGAATGTCC AGAATGGGAA GTGGGATTCA GATCCATCAG GGACCAAAAC CTGCATTGAT ACCAAGGAAG GCATCCTGCA GTATTGCCAA GAAGTCTACC CTGAACTGCA GATCACCAAT GTGGTAGAAG CCAACCAACC AGTGACCATC CAGAACTGGT GCAAGCGGGG CCGCAAGCAG TGCAAGACCC ATCCCCACTT TGTGATTCCC TACCGCTGCT TAGTTGGTGA GTTTGTAAGT GATGCCCTTC TCGTTCCTGA CAAGTGCAAA TTCTTACACC AGGAGAGGAT GGATGTTTGC GAAACTCATC TTCACTGGCA CACCGTCGCC AAAGAGACAT GCAGTGAGAA GAGTACCAAC TTGCATGACT ACGGCATGTT GCTGCCCTGC GGAATTGACA AGTTCCGAGG GGTAGAGTTT GTGTGTTGCC CACTGGCTGA AGAAAGTGAC AATGTGGATT CTGCTGATGC GGAGGAGGAT GACTCGGATG TCTGGTGGGG CGGAGCAGAC ACAGACTATG CAGATGGGAG TGAAGACAAA GTAGTAGAAG TAGCAGAGGA GGAAGAAGTG GCTGAGGTGG AAGAAGAAGA AGCCGATGAT GACGAGGACG ATGAGGATGG TGATGAGGTA GAGGAAGAGG CTGAGGAACC CTACGAAGAA GCCACAGAGA GAACCACCAG CATTGCCACC ACCACCACCA CCACCACAGA GTCTGTGGAA GAGGTGGTTC GAGTTCCTAC AACAGCAGCC AGTACCCCTG ATGCCGTTGA CAAGTATCTC GAGACACCTG GGGATGAGAA TGAACATGCC CATTTCCAGA AAGCCAAAGA GAGGCTTGAG GCCAAGCACC GAGAGAGAAT GTCCCAGGTC ATGAGAGAAT GGGAAGAGGC AGAACGTCAA GCAAAGAACT TGCCTAAAGC TGATAAGAAG GCAGTTATCC AGCATTTCCA GGAGAAAGTG GAATCTTTGG AACAGGAAGC AGCCAACGAG AGACAGCAGC TGGTGGAGAC ACACATGGCC AGAGTGGAAG CCATGCTCAA TGACCGCCGC CGCCTGGCCC TGGAGAACTA CATCACCGCT CTGCAGGCTG TTCCTCCTCG GCCTCGTCAC GTGTTCAATA TGCTAAAGAA GTATGTCCGC GCAGAACAGA AGGACAGACA GCACACCCTA AAGCATTTCG AGCATGTGCG CATGGTGGAT CCCAAGAAAG CCGCTCAGAT CCGGTCCCAG GTTATGACAC ACCTCCGTGT GATTTATGAG CGCATGAATC AGTCTCTCTC CCTGCTCTAC AACGTGCCTG CAGTGGCCGA GGAGATTCAG GATGAAGTTG ATGAGCTGCT TCAGAAAGAG CAAAACTATT CAGATGACGT CTTGGCCAAC ATGATTAGTG AACCAAGGAT CAGTTACGGA AACGATGCTC TCATGCCATC TTTGACCGAA ACGAAAACCA CCGTGGAGCT CCTTCCCGTG AATGGAGAGT TCAGCCTGGA CGATCTCCAG CCGTGGCATT CTTTTGGGGC TGACTCTGTG CCAGCCAACA CAGAAAACGA AGTTGAGCCT GTTGATGCCC GCCCTGCTGC CGACCGAGGA CTGACCACTC GACCAGGTTC TGGGTTGACA AATATCAAGA CGGAGGAGAT CTCTGAAGTG AATCTGGATG CAGAATTCCG ACATGACTCA GGATATGAAG TTCATCATCA AAAATTGGTG TTCTTTGCAG AAGATGTGGG TTCAAACAAA GGTGCAATCA TTGGACTCAT GGTGGGCGGT GTTGTCATAG CGACAGTGAT CGTCATCACC TTGGTGATGC TGAAGAAGAA ACAGTACACA TCCATTCATC ATGGTGTGGT GGAGGTTGAC GCCGCTGTCA CCCCAGAGGA GCGCCACCTG TCCAAGATGC AGCAGAACGG CTACGAAAAT CCAACCTACA AGTTCTTTGA GCAGATGCAG AACGATTACA AGGACGACGA TGACAAGTGA TCCAGACCAC CTCCCCTGCG AGCTAAGCTG GACAGCCAAT GACGGGTAAG AGAGTGACAT TTTTCACTAA CCTAAGACAG GAGGGCCGTC AGAGCTACTG CCTAATCCAA AGACGGGTAA AAGTGATAAA AATGTATCAC TCCAACCTAA GACAGGCGCA GCTTCCGAGG GATTTGAGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA AAACTAGAAT GCAGTGAAAA AAATGCCTTA TTTGTGAAAT TTGTGATGCT ATTGCCTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAAGTCGAT AAACTGGATC TCTGCTGTCC CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAGTTC TTTAGTTTGT ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA AGGCGATACC GTCGAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA CCCCAAATCC CCAGGAGCTG TTGATCCTTT AGGTATCTTT CCACAGCCAG GATTCTTGCC TGGAGCTGCT TGATGCCCCA GACTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC CTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACAATA ATTGTCTGGC CTGTACCGTC AGCGTCATTG ACGCTGCGCC CATAGTGCTT CCTGCTGCTC CCAAGAACCC AAGGAACAAA GCTCCTATTC CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC TAGTCAAAAT TTTTGGCGTA CTCACCAGTC GCCGCCCCTC GCCTCTTGCC GTGCGCGCTT CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT TTCAAGTCCC TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGGCAAAAAG CAGCTGCTTA TATGCAGGAT CTGAGGGCTC GCCACTCCCC AGTCCCGCCC AGGCCACGCC TCCCTGGAAA GTCCCCAGCG GAAAGTCCCT TGTAGCAAGC TCGATATCAG CAGTTCTTGA AGTACTCCGG ATGCAGCTCT CGGGCCACGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAACAC TTCTCTCTCC GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC TGGTGTTCTC TCCTTTATTG GCCTCTTCTA CCTTATCTGG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT GGATATCTGA CCCCTGGCCC TGGTGTGTAG TTCTGCTAAT CAGGGAAGTA GCCTTGTGTG TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCTTTGGG AGTGAATTAG CCCTTCCA
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