pMCS-Fluc-SV40-Rluc-IRES-Puro
$84.00
In stock
SKU
PL54147
100μg liquid plasmid(Instantly):$105
| name | pMCS-Fluc-SV40-Rluc-IRES-Puro | another name | apMCS-Fluc-SV40-Rluc-IRES-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Puro | Fluorescent labeling | Rluc,Fluc |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTTCG AAGGAGATAG AACCAGATCT TGGAATTCAG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC ATACGCTCTC CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA CATTTCTCTA CTAGTACGCG TATTTAAATG TCGACGTTTA AACTCTAGAG GATCCGCCAC CATGGCCGAT GCTAAGAACA TTAAGAAGGG CCCTGCTCCC TTCTACCCTC TGGAGGATGG CACCGCTGGC GAGCAGCTGC ACAAGGCCAT GAAGAGGTAT GCCCTGGTGC CTGGCACCAT TGCCTTCACC GATGCCCACA TTGAGGTGGA CATCACCTAT GCCGAGTACT TCGAGATGTC TGTGCGCCTG GCCGAGGCCA TGAAGAGGTA CGGCCTGAAC ACCAACCACC GCATCGTGGT GTGCTCTGAG AACTCTCTGC AGTTCTTCAT GCCAGTGCTG GGCGCCCTGT TCATCGGAGT GGCCGTGGCC CCTGCTAACG ACATTTACAA CGAGCGCGAG CTGCTGAACA GCATGGGCAT TTCTCAGCCT ACCGTGGTGT TCGTGTCTAA GAAGGGCCTG CAGAAGATCC TGAACGTGCA GAAGAAGCTG CCTATCATCC AGAAGATCAT CATCATGGAC TCTAAGACCG ACTACCAGGG CTTCCAGAGC ATGTACACAT TCGTGACATC TCATCTGCCT CCTGGCTTCA ACGAGTACGA CTTCGTGCCA GAGTCTTTCG ACAGGGACAA AACCATTGCC CTGATCATGA ACAGCTCTGG GTCTACCGGC CTGCCTAAGG GCGTGGCCCT GCCTCATCGC ACCGCCTGTG TGCGCTTCTC TCACGCCCGC GACCCTATTT TCGGCAACCA GATCATCCCC GACACCGCTA TTCTGAGCGT GGTGCCATTC CACCACGGCT TCGGCATGTT CACCACCCTG GGCTACCTGA TTTGCGGCTT TCGGGTGGTG CTGATGTACC GCTTCGAGGA GGAGCTGTTC CTGCGCAGCC TGCAAGACTA CAAAATTCAG TCTGCCCTGC TGGTGCCAAC CCTGTTCAGC TTCTTCGCTA AGAGCACCCT GATCGACAAG TACGACCTGT CTAACCTGCA CGAGATTGCC TCTGGCGGCG CCCCACTGTC TAAGGAGGTG GGCGAAGCCG TGGCCAAGCG CTTTCATCTG CCAGGCATCC GCCAGGGCTA CGGCCTGACC GAGACAACCA GCGCCATTCT GATTACCCCA GAGGGCGACG ACAAGCCTGG CGCCGTGGGC AAGGTGGTGC CATTCTTCGA GGCCAAGGTG GTGGACCTGG ACACCGGCAA GACCCTGGGA GTGAACCAGC GCGGCGAGCT GTGTGTGCGC GGCCCTATGA TTATGTCCGG CTACGTGAAT AACCCTGAGG CCACAAACGC CCTGATCGAC AAGGACGGCT GGCTGCACTC TGGCGACATT GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GCCTGAAGTC TCTGATCAAG TACAAGGGCT ACCAGGTGGC CCCAGCCGAG CTGGAGTCTA TCCTGCTGCA GCACCCTAAC ATTTTCGACG CCGGAGTGGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCTGCCGCC GTCGTCGTGC TGGAACACGG CAAGACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTGA CAACCGCCAA GAAGCTGCGC GGCGGAGTGG TGTTCGTGGA CGAGGTGCCC AAGGGCCTGA CCGGCAAGCT GGACGCCCGC AAGATCCGCG AGATCCTGAT CAAGGCTAAG AAAGGCGGCA AGATCGCCGT GTAACTCGAG GCAATAAAAT ATCTTATTTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCTTAATT AACTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CGGCGCGCCA TACCGGTGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAG CGGCCGCGAT CCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAAGC TTGCCACAAC CCACAAGGAG ACGACCTTCC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG CACGACCCCA TGCATCGGTA CCTAGAGTCG GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGCT GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACATT ATTCAGATTG GGCCCCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT
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