pCMV-CIITA(human)-2-3×FLAG-SV40-Neo
$99.00
In stock
SKU
PL54012
100μg liquid plasmid(Instantly):$120
| name | pCMV-CIITA(human)-2-3×FLAG-SV40-Neo | another name | apCMV-CIITA(human)-2-3×FLAG-SV40-Neo |
| Insert Size (bp) | 3390bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGGT ACCGAGCTCG GATCCGCCAC CATGCGTTGC CTGGCTCCAC GCCCTGCTGG GTCCTACCTG TCAGAGCCCC AAGGCAGCTC ACAGTGTGCC ACCATGGAGT TGGGGCCCCT AGAAGGTGGC TACCTGGAGC TTCTTAACAG CGATGCTGAC CCCCTGTGCC TCTACCACTT CTATGACCAG ATGGACCTGG CTGGAGAAGA AGAGATTGAG CTCTACTCAG AACCCGACAC AGACACCATC AACTGCGACC AGTTCAGCAG GCTGTTGTGT GACATGGAAG GTGATGAAGA GACCAGGGAG GCTTATGCCA ATATCGCGGA ACTGGACCAG TATGTCTTCC AGGACTCCCA GCTGGAGGGC CTGAGCAAGG ACATTTTCAA GCACATAGGA CCAGATGAAG TGATCGGTGA GAGTATGGAG ATGCCAGCAG AAGTTGGGCA GAAAAGTCAG AAAAGACCCT TCCCAGAGGA GCTTCCGGCA GACCTGAAGC ACTGGAAGCC AGCTGAGCCC CCCACTGTGG TGACTGGCAG TCTCCTAGTG GGACCAGTGA GCGACTGCTC CACCCTGCCC TGCCTGCCAC TGCCTGCGCT GTTCAACCAG GAGCCAGCCT CCGGCCAGAT GCGCCTGGAG AAAACCGACC AGATTCCCAT GCCTTTCTCC AGTTCCTCGT TGAGCTGCCT GAATCTCCCT GAGGGACCCA TCCAGTTTGT CCCCACCATC TCCACTCTGC CCCATGGGCT CTGGCAAATC TCTGAGGCTG GAACAGGGGT CTCCAGTATA TTCATCTACC ATGGTGAGGT GCCCCAGGCC AGCCAAGTAC CCCCTCCCAG TGGATTCACT GTCCACGGCC TCCCAACATC TCCAGACCGG CCAGGCTCCA CCAGCCCCTT CGCTCCATCA GCCACTGACC TGCCCAGCAT GCCTGAACCT GCCCTGACCT CCCGAGCAAA CATGACAGAG CACAAGACGT CCCCCACCCA ATGCCCGGCA GCTGGAGAGG TCTCCAACAA GCTTCCAAAA TGGCCTGAGC CGGTGGAGCA GTTCTACCGC TCACTGCAGG ACACGTATGG TGCCGAGCCC GCAGGCCCGG ATGGCATCCT AGTGGAGGTG GATCTGGTGC AGGCCAGGCT GGAGAGGAGC AGCAGCAAGA GCCTGGAGCG GGAACTGGCC ACCCCGGACT GGGCAGAACG GCAGCTGGCC CAAGGAGGCC TGGCTGAGGT GCTGTTGGCT GCCAAGGAGC ACCGGCGGCC GCGTGAGACA CGAGTGATTG CTGTGCTGGG CAAAGCTGGT CAGGGCAAGA GCTATTGGGC TGGGGCAGTG AGCCGGGCCT GGGCTTGTGG CCGGCTTCCC CAGTACGACT TTGTCTTCTC TGTCCCCTGC CATTGCTTGA ACCGTCCGGG GGATGCCTAT GGCCTGCAGG ATCTGCTCTT CTCCCTGGGC CCACAGCCAC TCGTGGCGGC CGATGAGGTT TTCAGCCACA TCTTGAAGAG ACCTGACCGC GTTCTGCTCA TCCTAGACGG CTTCGAGGAG CTGGAAGCGC AAGATGGCTT CCTGCACAGC ACGTGCGGAC CGGCACCGGC GGAGCCCTGC TCCCTCCGGG GGCTGCTGGC CGGCCTTTTC CAGAAGAAGC TGCTCCGAGG TTGCACCCTC CTCCTCACAG CCCGGCCCCG GGGCCGCCTG GTCCAGAGCC TGAGCAAGGC CGACGCCCTA TTTGAGCTGT CCGGCTTCTC CATGGAGCAG GCCCAGGCAT ACGTGATGCG CTACTTTGAG AGCTCAGGGA TGACAGAGCA CCAAGACAGA GCCCTGACGC TCCTCCGGGA CCGGCCACTT CTTCTCAGTC ACAGCCACAG CCCTACTTTG TGCCGGGCAG TGTGCCAGCT CTCAGAGGCC CTGCTGGAGC TTGGGGAGGA CGCCAAGCTG CCCTCCACGC TCACGGGACT CTATGTCGGC CTGCTGGGCC GTGCAGCCCT CGACAGCCCC CCCGGGGCCC TGGCAGAGCT GGCCAAGCTG GCCTGGGAGC TGGGCCGCAG ACATCAAAGT ACCCTACAGG AGGACCAGTT CCCATCCGCA GACGTGAGGA CCTGGGCGAT GGCCAAAGGC TTAGTCCAAC ACCCACCGCG GGCCGCAGAG TCCGAGCTGG CCTTCCCCAG CTTCCTCCTG CAATGCTTCC TGGGGGCCCT GTGGCTGGCT CTGAGTGGCG AAATCAAGGA CAAGGAGCTC CCGCAGTACC TAGCATTGAC CCCAAGGAAG AAGAGGCCCT ATGACAACTG GCTGGAGGGC GTGCCACGCT TTCTGGCTGG GCTGATCTTC CAGCCTCCCG CCCGCTGCCT GGGAGCCCTA CTCGGGCCAT CGGCGGCTGC CTCGGTGGAC AGGAAGCAGA AGGTGCTTGC GAGGTACCTG AAGCGGCTGC AGCCGGGGAC ACTGCGGGCG CGGCAGCTGC TGGAGCTGCT GCACTGCGCC CACGAGGCCG AGGAGGCTGG AATTTGGCAG CACGTGGTAC AGGAGCTCCC CGGCCGCCTC TCTTTTCTGG GCACCCGCCT CACGCCTCCT GATGCACATG TACTGGGCAA GGCCTTGGAG GCGGCGGGCC AAGACTTCTC CCTGGACCTC CGCAGCACTG GCATTTGCCC CTCTGGATTG GGGAGCCTCG TGGGACTCAG CTGTGTCACC CGTTTCAGGG CTGCCTTGAG CGACACAGTG GCGCTGTGGG AGTCCCTGCA GCAGCATGGG GAGACCAAGC TACTTCAGGC AGCAGAGGAG AAGTTCACCA TCGAGCCTTT CAAAGCCAAG TCCCTGAAGG ATGTGGAAGA CCTGGGAAAG CTTGTGCAGA CTCAGAGGAC GAGAAGTTCC TCGGAAGACA CAGCTGGGGA GCTCCCTGCT GTTCGGGACC TAAAGAAACT GGAGTTTGCG CTGGGCCCTG TCTCAGGCCC CCAGGCTTTC CCCAAACTGG TGCGGATCCT CACGGCCTTT TCCTCCCTGC AGCATCTGGA CCTGGATGCG CTGAGTGAGA ACAAGATCGG GGACGAGGGT GTCTCGCAGC TCTCAGCCAC CTTCCCCCAG CTGAAGTCCT TGGAAACCCT CAATCTGTCC CAGAACAACA TCACTGACCT GGGTGCCTAC AAACTCGCCG AGGCCCTGCC TTCGCTCGCT GCATCCCTGC TCAGGCTAAG CTTGTACAAT AACTGCATCT GTGACGTGGG AGCCGAGAGC TTGGCTCGTG TGCTTCCGGA CATGGTGTCC CTCCGGGTGA TGGACGTCCA GTACAACAAG TTCACGGCTG CCGGGGCCCA GCAGCTCGCT GCCAGCCTTC GGAGGTGTCC TCATGTGGAG ACGCTGGCGA TGTGGACGCC CACCATCCCA TTCAGTGTCC AGGAACACCT GCAACAACAG GATTCACGGA TCAGCCTGAG ACTCGAGTCT AGAGGGCCCT TCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTG AGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TC
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