NM_032217.5;CAGS; GTAR; MASK2; NY-BR-16
100μg liquid plasmid(Instantly):$192
| name | pCMV-mCherry-ANKRD17(human)-Myc-FLAG-Neo | another name | apCMV-mCherry-ANKRD17(human)-Myc-FLAG-Neo |
| Insert Size (bp) | 7812bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GGGAGGAGGG GGAAGCATGG AGAAGGCGAC GGTTCCGGTG GCGGCGGCGA CGGCTGCAGA AGGAGAAGGG AGCCCCCCGG CGGTGGCGGC TGTGGCGGGC CCCCCCGCGG CGGCGGAGGT CGGCGGCGGC GTTGGCGGCA GCAGCAGAGC TCGCTCGGCC TCGTCTCCTC GTGGGATGGT GCGAGTCTGC GACCTGCTCC TGAAGAAGAA GCCGCCGCAG CAGCAGCACC ACAAGGCCAA GCGTAACCGG ACTTGCCGAC CCCCCAGCAG CAGCGAAAGC AGCAGCGACA GCGACAACAG CGGCGGCGGT GGAGGCGGCG GTGGAGGCGG AGGTGGCGGC GGCGGCACCA GCAGTAACAA CAGCGAGGAA GAAGAGGACG ACGACGACGA GGAAGAGGAG GTTTCTGAGG TGGAGTCTTT CATTTTGGAT CAGGATGATT TGGAAAATCC AATGCTGGAA ACAGCTTCCA AGTTGCTCTT ATCAGGTACT GCTGATGGTG CAGACCTCAG GACAGTAGAT CCAGAAACAC AGGCTAGACT GGAAGCTTTA CTAGAAGCTG CAGGAATAGG AAAATTGTCC ACGGCTGATG GTAAAGCCTT TGCAGATCCT GAAGTACTTC GGAGGTTGAC ATCGTCTGTT AGTTGTGCGT TGGATGAAGC TGCTGCTGCA CTTACCCGTA TGAGAGCTGA AAGCACAGCA AATGCAGGGC AGTCGGACAA CCGCAGTTTG GCAGAAGCCT GTTCAGAAGG AGATGTAAAT GCTGTGCGAA AGTTACTCAT TGAAGGGCGA AGTGTAAATG AACACACAGA GGAAGGGGAG AGCCTCCTTT GTTTAGCTTG TTCTGCTGGA TACTATGAGC TTGCACAGGT TTTGTTGGCA ATGCATGCAA ATGTGGAAGA TAGGGGAATC AAAGGTGACA TTACACCTTT AATGGCTGCT GCTAATGGAG GACATGTCAA AATTGTGAAG TTGCTGCTAG CTCATAAAGC AGATGTTAAT GCACAGTCTT CAACAGGCAA TACAGCACTT ACATATGCTT GTGCTGGAGG CTATGTAGAT GTTGTAAAGG TGCTCTTGGA ATCCGGTGCT AGTATTGAGG ACCATAATGA AAATGGTCAT ACCCCTCTTA TGGAAGCTGG AAGTGCTGGA CATGTGGAAG TAGCCAGATT GCTGCTAGAA AATGGGGCTG GCATTAATAC GCATTCTAAT GAATTTAAAG AGAGTGCCCT TACCTTAGCT TGTTACAAAG GACACTTAGA GATGGTGCGA TTTCTTTTGG AAGCAGGCGC GGATCAAGAG CATAAAACAG ATGAAATGCA CACTGCTCTG ATGGAGGCTT GCATGGATGG CCATGTTGAA GTAGCTAGGT TACTTCTTGA CAGCGGTGCC CAAGTGAACA TGCCTGCTGA TTCATTTGAG TCACCATTAA CTTTGGCTGC ATGTGGTGGG CATGTGGAAC TTGCGGCTTT ACTTATTGAA AGAGGAGCTA GCCTGGAAGA GGTCAATGAT GAAGGTTATA CACCATTGAT GGAAGCAGCT CGAGAAGGAC ATGAAGAAAT GGTGGCATTA CTTCTTGGTC AAGGAGCAAA TATCAATGCA CAGACAGAAG AAACTCAAGA AACTGCCTTG ACTCTGGCTT GCTGTGGAGG CTTTCTGGAA GTGGCAGACT TTCTAATTAA GGCAGGAGCC GATATAGAAC TAGGGTGTTC TACCCCTTTA ATGGAAGCTG CTCAAGAGGG TCATTTGGAG TTAGTTAAAT ACTTATTAGC TGCAGGAGCT AACGTTCATG CAACAACAGC AACAGGGGAT ACAGCACTAA CATATGCCTG TGAAAATGGT CATACTGATG TAGCAGATGT CTTACTTCAG GCAGGCGCAG ATCTGGAACA TGAATCTGAA GGTGGAAGAA CTCCTTTAAT GAAAGCTGCA AGAGCTGGTC ATGTTTGTAC TGTTCAGTTC TTAATTAGTA AAGGAGCGAA TGTGAATAGA ACCACAGCTA ATAATGACCA TACTGTACTG TCCCTGGCTT GTGCAGGGGG TCATCTGGCA GTGGTGGAAC TACTTTTGGC TCATGGGGCA GATCCTACTC ACCGTTTGAA AGATGGCTCA ACTATGTTGA TAGAAGCAGC AAAAGGTGGC CATACAAGTG TTGTTTGCTA TCTCTTGGAT TATCCTAATA ACTTGCTTTC AGCCCCTCCA CCAGATGTCA CTCAGTTAAC TCCCCCATCC CACGATTTAA ATAGGGCTCC TCGTGTACCA GTTCAAGCAC TGCCCATGGT TGTTCCACCT CAGGAGCCTG ACAAACCACC TGCCAATGTT GCCACCACTC TTCCCATCAG GAATAAAGCT GCTTCTAAAC AAAAGTCCAG CAGCCATTTG CCAGCAAACA GCCAGGATGT ACAGGGTTAC ATCACCAATC AGTCTCCAGA GAGCATTGTA GAAGAGGCTC AGGGAAAGTT AACAGAACTG GAACAGAGGA TAAAAGAAGC CATAGAAAAG AATGCACAGC TGCAGTCCTT GGAACTGGCT CATGCTGACC AACTTACCAA GGAGAAGATC GAGGAGCTCA ACAAAACAAG GGAGGAACAA ATTCAGAAGA AACAAAAGAT TTTGGAGGAA CTACAGAAAG TAGAACGAGA GTTACAACTG AAAACTCAGC AGCAGCTAAA AAAGCAGTAT CTAGAGGTTA AAGCTCAAAG AATTCAACTT CAGCAACAGC AGCAACAGTC TTGCCAACAC CTGGGATTAC TAACTCCTGT TGGAGTTGGA GAGCAGCTTT CTGAGGGAGA CTATGCACGG TTACAGCAAG TGGATCCTGT TTTACTTAAA GATGAACCCC AGCAGACTGC TGCTCAGATG GGTTTTGCGC CAATCCAGCC TCTGGCGATG CCTCAAGCTT TGCCTCTGGC GGCAGGTCCC TTGCCTCCAG GGTCCATCGC AAATCTTACA GAACTGCAAG GAGTGATAGT TGGACAGCCA GTACTGGGCC AAGCACAGTT GGCAGGGCTG GGGCAAGGAA TTCTGACAGA AACACAACAA GGGTTAATGG TAGCCAGCCC TGCTCAGACC CTCAATGACA CGCTGGATGA CATCATGGCA GCAGTCAGTG GAAGAGCATC TGCAATGTCA AACACTCCTA CCCACAGTAT TGCTGCATCC ATTTCCCAAC CTCAGACTCC AACTCCAAGT CCTATCATCT CTCCTTCAGC CATGCTTCCT ATCTACCCTG CCATTGATAT TGATGCACAG ACTGAGAGTA ATCATGACAC GGCACTAACA CTTGCCTGTG CTGGTGGCCA CGAGGAACTG GTACAAACAC TGCTAGAGAG AGGAGCTAGT ATAGAGCACC GAGACAAGAA AGGTTTTACT CCACTCATCT TGGCTGCCAC AGCTGGTCAT GTTGGTGTTG TGGAAATATT GCTGGACAAT GGTGCAGACA TTGAAGCCCA GTCTGAAAGA ACCAAGGACA CACCACTCTC CTTGGCTTGT TCTGGGGGAA GACAGGAGGT GGTGGAGCTA TTGTTAGCTC GAGGGGCAAA TAAAGAGCAC AGGAATGTTT CTGATTACAC ACCTCTAAGT CTGGCTGCTT CTGGTGGCTA TGTGAACATC ATCAAAATAT TACTAAATGC AGGAGCTGAG ATTAACTCTA GAACTGGTAG CAAATTGGGC ATCTCTCCTC TGATGTTAGC AGCTATGAAT GGGCATACAG CTGCTGTTAA GCTCCTGTTA GACATGGGCT CTGACATAAA TGCTCAGATA GAAACCAATC GGAACACTGC CCTTACTTTA GCCTGCTTCC AAGGAAGAAC TGAAGTGGTT AGTCTTCTGC TTGATAGAAA AGCAAATGTT GAACACAGAG CTAAGACTGG TCTCACACCA CTAATGGAAG CTGCCTCTGG TGGATATGCG GAGGTGGGCC GAGTTCTTTT GGATAAAGGT GCTGATGTTA ATGCCCCTCC AGTTCCCTCC TCAAGAGATA CAGCTTTAAC CATAGCAGCA GATAAAGGGC ATTACAAATT CTGTGAGCTT CTTATTGGCA GGGGAGCTCA TATTGATGTA CGTAACAAGA AGGGGAACAC TCCATTGTGG CTAGCAGCAA ATGGTGGACA CCTCGATGTG GTTCAGTTAC TGGTGCAAGC AGGTGCAGAT GTGGATGCAG CAGATAACCG CAAGATAACT CCTCTTATGG CAGCATTTAG AAAGGGTCAT GTGAAGGTGG TGCGCTACTT AGTCAAAGAA GTCAATCAGT TTCCATCAGA TTCTGAATGT ATGAGATACA TAGCAACCAT CACTGATAAG GAGATGCTGA AGAAGTGTCA TCTTTGTATG GAGTCAATAG TACAAGCCAA AGATAGACAG GCTGCTGAAG CAAACAAAAA CGCCAGCATT TTGTTAGAGG AGTTAGACTT GGAAAAGTTA AGGGAAGAAA GTCGGAGGCT GGCTTTGGCT GCGAAAAGAG AAAAAAGAAA AGAGAAGAGA AGGAAGAAAA AGGAAGAACA AAGAAGGAAA CTAGAAGAAA TTGAAGCCAA AAATAAAGAG AACTTTGAAC TCCAAGCTGC TCAAGAAAAA GAAAAGCTTA AAGTTGAAGA TGAGCCTGAA GTCTTGACAG AACCTCCAAG TGCCACAACC ACTACTACCA TAGGTATATC TGCAACCTGG ACAACTTTGG CAGGTTCTCA TGGTAAAAGA AATAATACCA TAACTACAAC CAGTTCAAAG AGGAAAAACA GGAAAAATAA AATTACTCCA GAAAACGTTC AAATTATATT TGATGATCCA CTACCAATTT CATACAGTCA GCCAGAGAAG GTGAATGGAG AGTCCAAGAG CAGCAGTACC AGCGAGAGTG GGGACAGTGA TAACATGAGG ATTTCCAGCT GCAGCGATGA AAGTAGTAAC AGCAACAGCA GTCGTAAGAG TGACAATCAT TCACCAGCTG TGGTCACTAC CACTGTGAGC AGCAAAAAGC AGCCATCAGT TCTTGTTACA TTTCCAAAGG AAGAGAGAAA ATCTGTTTCT GGCAAGGCTT CAATAAAATT GTCAGAAACT ATCAGTGAAG GGACCAGTAA TTCTCTATCT ACTTGTACAA AATCTGGTCC ATCTCCCCTT TCTTCTCCAA ATGGGAAGTT AACAGTAGCA AGTCCTAAGC GTGGGCAAAA GAGGGAAGAA GGATGGAAAG AAGTTGTAAG AAGGTCAAAG AAAGTATCTG TTCCATCAAC TGTGATATCC AGAGTGATTG GAAGAGGAGG CTGTAATATC AATGCTATTC GGGAGTTTAC TGGTGCACAC ATAGATATTG ATAAACAGAA AGACAAGACT GGAGACCGGA TAATCACTAT AAGGGGTGGC ACTGAATCAA CAAGACAAGC AACTCAATTG ATTAATGCTT TGATCAAGGA TCCAGACAAA GAAATTGATG AACTTATTCC AAAGAATCGT TTGAAAAGCT CCTCAGCAAA TTCCAAAATA GGGTCATCAG CACCTACCAC CACTGCTGCT AACACTTCCT TAATGGGAAT TAAAATGACA ACTGTAGCTC TGTCATCAAC ATCTCAAACT GCCACAGCAC TCACTGTGCC TGCAATTTCT TCTGCATCCA CTCACAAAAC CATTAAGAAC CCAGTGAATA ATGTGAGGCC TGGTTTTCCA GTTTCTCTTC CATTAGCATA TCCTCCTCCA CAGTTTGCAC ATGCTTTGCT TGCTGCTCAG ACTTTCCAGC AGATCCGTCC ACCAAGGTTG CCCATGACCC ACTTTGGAGG TACTTTTCCA CCAGCTCAAT CCACTTGGGG TCCGTTTCCT GTCAGGCCTT TGAGCCCTGC CAGAGCTACT AACTCGCCTA AGCCTCACAT GGTGCCTCGC CATAGCAATC AGAATAGCAG TGGTTCTCAG GTGAATTCAG CAGGTTCTTT AACTTCAAGC CCAACAACTA CAACCAGTTC ATCAGCTTCA ACGGTGCCTG GTACATCTAC AAATGGCAGT CCAAGTTCAC CTTCTGTCCG AAGGCAGCTT TTTGTCACAG TTGTGAAGAC ATCCAATGCC ACCACAACAA CAGTCACAAC CACGGCAAGC AACAACAACA CTGCACCCAC AAATGCCACA TATCCTATGC CTACTGCCAA AGAACACTAT CCAGTATCAT CCCCATCTTC CCCATCACCA CCAGCCCAGC CAGGAGGGGT TTCTAGAAAC AGCCCTTTGG ATTGTGGATC AGCATCTCCA AATAAAGTGG CATCTTCCTC CGAACAGGAA GCAGGTAGTC CACCAGTAGT AGAAACAACA AACACTAGAC CTCCAAACAG CAGCAGTTCT TCTGGGAGTT CATCAGCTCA TTCTAATCAG CAACAACCTC CGGGATCTGT TTCTCAGGAA CCAAGACCAC CTCTTCAGCA GTCTCAGGTT CCTCCCCCGG AAGTTAGAAT GACTGTTCCT CCTTTAGCAA CAAGTTCTGC TCCAGTGGCG GTGCCTTCTA CTGCCCCAGT GACTTACCCT ATGCCTCAGA CACCAATGGG ATGCCCCCAG CCTACTCCTA AAATGGAAAC CCCTGCTATT AGACCACCCC CTCATGGCAC AACTGCCCCT CACAAGAATT CAGCTTCAGT GCAAAATTCA TCTGTTGCAG TCCTCAGTGT CAATCACATT AAAAGACCTC ACAGTGTTCC CTCTTCTGTC CAGCTACCTT CGACCTTAAG TACACAAAGT GCTTGTCAGA ATTCAGTACA TCCAGCAAAT AAGCCTATTG CTCCCAATTT CAGTGCCCCC TTACCATTTG GGCCCTTTAG CACATTGTTT GAAAACAGCC CTACTTCTGC TCATGCCTTC TGGGGAGGAT CTGTTGTTTC ATCTCAGTCA ACACCAGAAT CTATGCTATC AGGAAAATCC TCATATTTGC CAAATTCAGA TCCTTTACAT CAGTCTGATA CTTCCAAAGC TCCAGGTTTT AGACCACCAT TACAGAGACC TGCTCCAAGT CCCTCAGGTA TTGTCAATAT GGACTCGCCA TATGGTTCTG TAACACCTTC TTCAACACAT TTGGGAAACT TTGCTTCAAA CATTTCAGGA GGTCAGATGT ACGGACCTGG GGCACCCCTT GGAGGAGCAC CCGCAGCTGC TAACTTTAAC AGACAACATT TTTCCCCGCT TAGTTTGTTG ACTCCGTGTT CATCAGCATC AAATGATTCT TCTGCACAGT CAGTATCCTC GGGAGTTCGT GCACCATCTC CTGCCCCATC ATCAGTACCG TTAGGGTCAG AAAAGCCCAG CAATGTGTCT CAGGACAGGA AAGTTCCAGT CCCTATTGGG ACTGAACGTT CTGCACGTAT CAGGCAAACT GGAACGTCAG CTCCATCTGT TATTGGGAGC AATTTGTCTA CATCAGTAGG ACATAGTGGC ATCTGGTCCT TTGAAGGGAT TGGTGGCAAT CAAGACAAAG TAGACTGGTG TAACCCTGGG ATGGGAAATC CTATGATCCA CAGACCGATG TCTGACCCAG GAGTATTTTC ACAACATCAA GCAATGGAGC GAGATAGTAC AGGAATTGTA ACTCCTTCTG GTACATTCCA TCAGCATGTT CCTGCAGGCT ACATGGACTT TCCTAAAGTT GGGGGTATGC CTTTTTCTGT GTATGGGAAT GCAATGATTC CTCCAGTAGC ACCTATCCCT GATGGTGCTG GAGGACCCAT ATTTAATGGC CCTCATGCTG CAGACCCTTC TTGGAACTCA CTGATAAAGA TGGTTTCCAG CTCCACGGAA AATAATGGCC CTCAAACGGT GTGGACTGGA CCCTGGGCAC CTCACATGAA CAGTGTGCAT ATGAACCAGC TTGGCCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
