pUT-mini-Tn5-Gm-Tac-EGFP-SacB
$128.00
In stock
SKU
PL53931
100μg liquid plasmid(Instantly):$171
| name | pUT-mini-Tn5-Gm-Tac-EGFP-SacB | another name | apUT-mini-Tn5-Gm-Tac-EGFP-SacB |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Tac | Replicon | R6K |
| Competence | S17-1λpir | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCCTTTTT GTCCGGTGTT GGGTTGAAGG TGAAGCCGGT CGGGGCCGCA GCGGGGGCCG GCTTTTCAGC CTTGCCCCCC TGCTTCGGCC GCCGTGGCTC CGGCGTCTTG GGTGCCGGCG CGGGTTCCGC AGCCTTGGCC TGCGGTGCGG GCACATCGGC GGGCTTGGCC TTGATGTGCC GCCTGGCGTG CGAGCGGAAC GTCTCGTAGG AGAACTTGAC CTTCCCCGTT TCCCGCATGT GCTCCCAAAT GGTGACGAGC GCATAGCCGG ACGCTAACGC CGCCTCGACA TCCGCCCTCA CCGCCAGGAA CGCAACCGCA GCCTCATCAC GCCGGCGCTT CTTGGCCGCG CGGGATTCAA CCCACTCGGC CAGCTCGTCG GTGTAGCTCT TTGGCATCGT CTCTCGCCTG TCCCCTCAGT TCAGTAATTT CCTGCATTTG CCTGTTTCCA GTCGGTAGAT ATTCCACAAA ACAGCAGGGA AGCAGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GAGCGCCTGG GTCACGTGCG CGTCACGAAC TGCGAGGCAA ACACCCTGCC CGCTGTCATG GCCGAGGTGA TGGCGACCCA GCACGGCAAC ACCCGTTCCG AGGCCGACAA GACCTATCAC CTGCTGGTTA GCTTCCGCGC GGGAGAGAAG CCCGACGCGG AGACGTTGCG CGCGATTGAG GACCGCATCT GCGCTGGGCT TGGCTTCGCC GAGCATCAGC GCGTCAGTGC CGTGCATCAC GACACCGACA ACCTGCACAT CCATATCGCC ATCAACAAGA TTCACCCGAC CCGAAACACC ATCCATGAGC CGTATCGGGC CTACCGCGCC CTCGCTGACC TCTGCGCGAC GCTCGAACGG GACTACGGGC TTGAGCGTGA CAATCACGAA ACGCGGCAGC GCGTTTCCGA GAACCGCGCG AACGACATGG AGCGGCACGC GGGCGTGGAA AGCCTGGTCG GCTGGATCCG GCCACGATGC GTCCGGCGTA GAGGATCTGA AGATCAGCAG TTCAACCTGT TGATAGTACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT AACGTACTAA GCTCTCATGT TTAACGAACT AAACCCTCAT GGCTAACGTA CTAAGCTCTC ATGGCTAACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT GAACAATAAA ATTAATATAA ATCAGCAACT TAAATAGCCT CTAAGGTTTT AAGTTTTATA AGAAAAAAAA GAATATATAA GGCTTTTAAA GCTTTTAAGG TTTAACGGTT GTGGACAACA AGCCAGGGAT GTAACGCACT GAGAAGCCCT TAGAGCCTCT CAAAGCAATT TTGAGTGACA CAGGAACACT TAACGGCTGA CATGGGAATT CCACATGTGG AATTCCACAT GTGGAATTGT GAGCGGATAA CAATTTGTGG AATTCCCGGG AGAGCTCCTG ACTCTTATAC ACAAGTTCGG CCACGGCCTA GGCGGCCGCT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAAATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAGGCGGC CGCAGCTTGT CGACGAATTC CCGGGGATCC GGTGATTGAT TGAGCAAGCT TTATGCTTGT AAACCGTTTT GTGAAAAAAT TTTTAAAATA AAAAAGGGGA CCTCTAGGGT CCCCAATTAA TTAGTAATAT AATCTATTAA AGGTCATTCA AAAGGTCATC CACCGGATCA ATTCCCCTGC TCACACAGAC TGGGTACCAA GCTCTCGGTA ACATCAAGGC CCGATCCTTG GAGCCCTTGC CCTCCCGACG ATGATCGTGC CGTGATCGAA ATCCAGATCC TTGACCCACA GTTGCAAACC CTCACTGATC CGTCCCGTCC GGGTCGACCA AAGCGGCCAT CGTGCCTCCC CACTCCTGCA GTTCGGGGGC ATGGATGCGC GGATAGCCGC TGCTGGTTTC CTGGATGCCG ACGGATTTGC ACTGCCGGTA GAACTCCGCG AGGTCGTCCA GCCTCAGGCA GCAGCTGAAC CAACTCGCGA GGGGATCGAG CCCGGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTTAG GTGGCGGTAC TTGGGTCGAT ATCAAAGTGC ATCACTTCTT CCCGTATGCC CAACTTTGTA TAGAGAGCCA CTGCGGGATC GTCACCGTAA TCTGCTTGCA CGTAGATCAC ATAAGCACCA AGCGCGTTGG CCTCATGCTT GAGGAGATTG ATGAGCGCGG TGGCAATGCC CTGCCTCCGG TGCTCGCCGG AGACTGCGAG ATCATAGATA TAGATCTCAC TACGCGGCTG CTCAAACCTG GGCAGAACGT AAGCCGCGAG AGCGCCAACA ACCGCTTCTT GGTCGAAGGC AGCAAGCGCG ATGAATGTCT TACTACGGAG CAAGTTCCCG AGGTAATCGG AGTCCGGCTG ATGTTGGGAG TAGGTGGCTA CGTCTCCGAA CTCACGACCG AAAAGATCAA GAGCAGCCCG CATGGATTTG ACTTGGTCAG GGCCGAGCCT ACATGTGCGA ATGATGCCCA TACTTGAGCC ACCTAACTTT GTTTTAGGGC GACTGCCCTG CTGCGTAACA TCGTTGCTGC TGCGTAACAT GCGAAACGAT CCTCATCCTG TCTCTTGATC AGATCTTGAT CCCCTGCGCC ATCAGATCCT TGGCGGCAAG AAAGCCATCC AGTTTACTTT GCAGGGCTTC CCAACCTTAC CAGAGGGCGC CCCAGCTGGC AATTCCGGTT CGCTTGCTGT CCATAAAACC GCCCAGTCTA GCTATCGTCA TGTAAGCCCA CTGCAAGCTA CCTCTTTCTC TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT CCGGGGTAGC ACCGTTTCTG CGGACTGGCT TTCTACGTGT TCCGCTTCCT TTAGCAGCCC TTGCGCCCTG ATGCTTGCGG CAGCGTGAAG CTCGCGCAGA TCAGTTGGTT GATTTTGTCC ACTACGTGAA AGGCGAATCA CCAAGGTAGT CGGCAAATAA TGTCTAACAA TTCGTTCAAG CCGACGCCGC TTCGCGGCGC GGTTAACTCA AGCGTTAGAT GCACTAAGCA CATAATTGCT CACAGCCAAA CTATCAGGTC AAGTCGCTTT TATTATTTTT AAGCGTGCAT AATAAGCCCT ACACAAAATT GGGAGATATA TCATGAAAGG CGCTTTTCTT GTTATCGCAA TAGTTGGGCG AAGTAATCGC AACATCCGCA TTAACAATCT AGCGAGGGCT TGGTAAGGTG ATCCGGTGGA TGACCTTTTG AATGACCTTT AATAGATTAT ATTACTAATT AATTGGGGAC CCTAGAGGTC CCCTTTTTTA TTTTAAAAAT TTTTTCACAA AACGGTTTAC AAGCATAAAG CTTGCTCAAT CAATCACCGG ATCCCCGGGA ATTCGGCCTA GGTGGCCAGA TCTGATCAAG AGACAGAAAC GCAAAAGAAA ATGCCGATAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTAACTTT AAGAAGGAGA TATACATATG ATTACCAGTG CACTGCATCG TGCGGCGGAT TGGGCGAAAA GCGTGTTTTC TAGTGCTGCG CTGGGTGATC CGCGTCGTAC CGCGCGTCTG GTGAATGTTG CGGCGCAACT GGCCAAATAT AGCGGCAAAA GCATTACCAT TAGCAGCGAA GGCAGCAAAG CCATGCAGGA AGGCGCGTAT CGTTTTATTC GTAATCCGAA CGTGAGCGCG GAAGCGATTC GTAAAGCGGG TGCCATGCAG ACCGTGAAAC TGGCCCAGGA ATTTCCGGAA CTGCTGGCAA TTGAAGATAC CACCTCTCTG AGCTATCGTC ATCAGGTGGC GGAAGAACTG GGCAAACTGG GTAGCATTCA GGATAAAAGC CGTGGTTGGT GGGTGCATAG CGTGCTGCTG CTGGAAGCGA CCACCTTTCG TACCGTGGGC CTGCTGCATC AAGAATGGTG GATGCGTCCG GATGATCCGG CGGATGCGGA TGAAAAAGAA AGCGGCAAAT GGCTGGCCGC TGCTGCAACT TCGCGTCTGA GAATGGGCAG CATGATGAGC AACGTGATTG CGGTGTGCGA TCGTGAAGCG GATATTCATG CGTATCTGCA AGATAAACTG GCCCATAACG AACGTTTTGT GGTGCGTAGC AAACATCCGC GTAAAGATGT GGAAAGCGGC CTGTATCTGT ATGATCACCT GAAAAACCAG CCGGAACTGG GCGGCTATCA GATTAGCATT CCGCAGAAAG GCGTGGTGGA TAAACGTGGC AAACGTAAAA ACCGTCCGGC GCGTAAAGCG AGCCTGAGCC TGCGTAGCGG CCGTATTACC CTGAAACAGG GCAACATTAC CCTGAACGCG GTGCTGGCCG AAGAAATTAA TCCGCCGAAA GGCGAAACCC CGCTGAAATG GCTGCTGCTG ACCAGCGAGC CGGTGGAAAG TCTGGCCCAA GCGCTGCGTG TGATTGATAT TTATACCCAT CGTTGGCGCA TTGAAGAATT TCACAAAGCG TGGAAAACGG GTGCGGGTGC GGAACGTCAG CGTATGGAAG AACCGGATAA CCTGGAACGT ATGGTGAGCA TTCTGAGCTT TGTGGCGGTG CGTCTGCTGC AACTGCGTGA ATCTTTTACT CCGCCGCAAG CACTGCGTGC GCAGGGCCTG CTGAAAGAAG CGGAACACGT TGAAAGCCAG AGCGCGGAAA CCGTGCTGAC CCCGGATGAA TGCCAACTGC TGGGCTATCT GGATAAAGGC AAACGCAAAC GCAAAGAAAA AGCGGGCAGC CTGCAATGGG CGTATATGGC GATTGCGCGT CTGGGCGGCT TTATGGATAG CAAACGTACC GGCATTGCGA GCTGGGGTGC GCTGTGGGAA GGTTGGGAAG CGCTGCAAAG CAAACTGGAT GGCTTTCTGG CCGCGAAAGA CCTGATGGCG CAGGGCATTA AAATCTAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGACGC GTGCTATCGT CATGTAAGCC CACTGATCGG CATTTTCTTT TGCGTTTTTA TTTGTTAACT GTTAATTGTC CTTGTTCAAG GATGCTGTCT TTGACAACAG ATGTTTTCTT GCCTTTGATG TTCAGCAGGA AGCTCGGCGC AAACGTTGAT TGTTTGTCTG CGTAGAATCC TCTGTTTGTC ATATAGCTTG TAATCACGAC ATTGTTTCCT TTCGCTTGAG GTACAGCGAA GTGTGAGTAA GTAAAGGTTA CATCGTTAGG ATCAAGATCC ATTTTTAACA CAAGGCCAGT TTTGTTCAGC GGCTTGTATG GGCCAGTTAA AGAATTAGAA ACATAACCAA GCATGTAAAT ATCGTTAGAC GTAATGCCGT CAATCGTCAT TTTTGATCCG CGGGAGTCAG TGAACAGGTA CCATTTGCCG TTCATTTTAA AGACGTTCGC GCGTTCAATT TCATCTGTTA CTGTGTTAGA TGCAATCAGC GGTTTCATCA CTTTTTTCAG TGTGTAATCA TCGTTTAGCT CAATCATACC GAGAGCGCCG TTTGCTAACT CAGCCGTGCG TTTTTTATCG CTTTGCAGAA GTTTTTGACT TTCTTGACGG AAGAATGATG TGCTTTTGCC ATAGTATGCT TTGTTAAATA AAGATTCTTC GCCTTGGTAG CCATCTTCAG TTCCAGTGTT TGCTTCAAAT ACTAAGTATT TGTGGCCTTT ATCTTCTACG TAGTGAGGAT CTCTCAGCGT ATGGTTGTCG CCTGAGCTGT AGTTGCCTTC ATCGATGAAC TGCTGTACAT TTTGATACGT TTTTCCGTCA CCGTCAAAGA TTGATTTATA ATCCTCTACA CCGTTGATGT TCAAAGAGCT GTCTGATGCT GATACGTTAA CTTGTGCAGT TGTCAGTGTT TGTTTGCCGT AATGTTTACC GGAGAAATCA GTGTAGAATA AACGGATTTT TCCGTCAGAT GTAAATGTGG CTGAACCTGA CCATTCTTGT GTTTGGTCTT TTAGGATAGA ATCATTTGCA TCGAATTTGT CGCTGTCTTT AAAGACGCGG CCAGCGTTTT TCCAGCTGTC AATAGAAGTT TCGCCGACTT TTTGATAGAA CATGTAAATC GATGTGTCAT CCGCATTTTT AGGATCTCCG GCTAATGCAA AGACGATGTG GTAGCCGTGA TAGTTTGCGA CAGTGCCGTC AGCGTTTTGT AATGGCCAGC TGTCCCAAAC GTCCAGGCCT TTTGCAGAAG AGATATTTTT AATTGTGGAC GAATCAAATT CAGAAACTTG ATATTTTTCA TTTTTTTGCT GTTCAGGGAT TTGCAGCATA TCATGGCGTG TAATATGGGA AATGCCGTAT GTTTCCTTAT ATGGCTTTTG GTTCGTTTCT TTCGCAAACG CTTGAGTTGC GCCTCCTGCC AGCAGTGCGG TAGTAAAGGT TAATACTGTT GCTTGTTTTG CAAACTTTTT GATGTTCATC GTTCATGTCT CCTTTTTTAT GTACTGTGTT AGCGGTCTGC TTCTTCCAGC CCTCCTGTTT GAAGATGGCA AGTTAGTTAC GCACAATAAA AAAAGACCTA AAATATGTAA GGGGTGACGC CAAAGTATAC ACTTTGCCCT TTACACATTT TAGGTCTTGC CTGCTTTATC AGTAACAAAC CCGCGCGATT TACTTTTCGA CCTCATTCTA TTAGACTCTC GTTTGGATTG CAACTGGTCT ATTTTCCTCT TTTGTTTGAT AGAAAATCAT AAAAGGATTT GCAGACTACG GGCCTAAAGA ACTAAAAAAT CTATCTGTTT CTTTTCATTC TCTGTATTTT TTATAGTTTC TGTTGCATGG GCATAAAGTT GCCTTTTTAA TCACAATTCA GAAAATATCA TAATATCTCA TTTCACTAAA TAATAGTGAA CGGCAGGTAT ATGTGGGTTA CCGACTGCGG CCTGAGTTTT TTAAGTGACG TAAAATCGTG TTGAGGCCAA CGCCCATAAT GCGGGCTGTT GCCCGGCATC CAACGCCATT CATGGCCATA TCAATGATTT TCTGGTGCGT ACCGGGTTGA GAAGCGGTGT AAGTGAACTG CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCA
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