pCMV-EIF2AK3(human)-SV40-Hyg
$99.00
In stock
SKU
PL53919
NM_001313915.2;NM_004836.7
100μg liquid plasmid(Instantly):$120
| name | pCMV-EIF2AK3(human)-SV40-Hyg | another name | apCMV-EIF2AK3(human)-SV40-Hyg |
| Insert Size (bp) | 3351bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG AGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCCTC ACTCTCTTCC GCATCGCTGT CTGCGAGGGC CAGCTGTTGG GCTCGCGGTT GAGGACAAAC TCTTCGCGGT CTTTCCAGTA CTCTTGGATC GGAAACCCGT CGGCCTCCGA ACGGTACTCC GCCACCGAGG GACCTGAGCG AGTCCGCATC GACCGGATCG GAAAACCTCT CGAGAAAGGC GTCTAACCAG TCACAGTCGC AAGGTAGGCT GAGCACCGTG GCGGGCGGCA GCGGGTGGCG GTCGGGGTTG TTTCTGGCGG AGGTGCTGCT GATGATGTAA TTAAAGTAGG CGGTCTTGAG ACGGCGGATG GTCGAGGTGA GGTGTGGCAG GCTTGAGATC CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATT ACTTCTGCGC TAAGATTGTC AGTTTCCAAA AACGAGGAGG ATTTGATATT CACCTGGCCC GATCTGGCCA TACACTTGAG TGACAATGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGG TCCAAGTTTA AACTTTAATA CGACTCACTA TAGGGGCCGC CACCAAGCTT GGTACCATGG AGCGCGCCAT CAGCCCGGGG CTGCTGGTAC GGGCGCTGCT GCTGCTGCTG CTGCTGCTGG GGCTCGCGGC AAGGACGGTG GCCGCGGGGC GCGCCCGTGG CCTCCCAGCG CCGACGGCGG AGGCGGCGTT CGGCCTCGGG GCGGCCGCTG CTCCCACCTC AGCGACGCGA GTACCGGCGG CGGGCGCCGT GGCTGCGGCC GAGGTGACTG TGGAGGACGC TGAGGCGCTG CCGGCAGCCG CGGGAGAGCA GGAGCCTCGG GGTCCGGAAC CAGACGATGA GACAGAGTTG CGACCGCGCG GCAGGTCATT AGTAATTATC AGCACTTTAG ATGGGAGAAT TGCTGCCTTG GATCCTGAAA ATCATGGTAA AAAGCAGTGG GATTTGGATG TGGGATCCGG TTCCTTGGTG TCATCCAGCC TTAGCAAACC AGAGGTATTT GGGAATAAGA TGATCATTCC TTCCCTGGAT GGAGCCCTCT TCCAGTGGGA CCAAGACCGT GAAAGCATGG AAACAGTTCC TTTCACAGTT GAATCACTTC TTGAATCTTC TTATAAATTT GGAGATGATG TTGTTTTGGT TGGAGGAAAA TCTCTGACTA CATATGGACT CAGTGCATAT AGTGGAAAGG TGAGGTATAT CTGTTCAGCT CTGGGTTGTC GCCAATGGGA TAGTGACGAA ATGGAACAAG AGGAAGACAT CCTGCTTCTA CAGCGTACCC AAAAAACTGT TAGAGCTGTC GGACCTCGCA GTGGCAATGA GAAGTGGAAT TTCAGTGTTG GCCACTTTGA ACTTCGGTAT ATTCCAGACA TGGAAACGAG AGCCGGATTT ATTGAAAGCA CCTTTAAGCC CAATGAGAAC ACAGAAGAGT CTAAAATTAT TTCAGATGTG GAAGAACAGG AAGCTGCCAT AATGGACATA GTGATAAAGG TTTCGGTTGC TGACTGGAAA GTTATGGCAT TCAGTAAGAA GGGAGGACAT CTGGAATGGG AGTACCAGTT TTGTACTCCA ATTGCATCTG CCTGGTTACT TAAGGATGGG AAAGTCATTC CCATCAGTCT TTTTGATGAT ACAAGTTATA CATCTAATGA TGATGTTTTA GAAGATGAAG AAGACATTGT AGAAGCTGCC AGAGGAGCCA CAGAAAACAG TGTTTACTTG GGAATGTATA GAGGCCAGCT GTATCTGCAG TCATCAGTCA GAATTTCAGA AAAGTTTCCT TCAAGTCCCA AGGCTTTGGA ATCTGTCACT AATGAAAACG CAATTATTCC TTTACCAACA ATCAAATGGA AACCCTTAAT TCATTCTCCT TCCAGAACTC CTGTCTTGGT AGGATCTGAT GAATTTGACA AATGTCTCAG TAATGATAAG TTTTCTCATG AAGAATATAG TAATGGTGCA CTTTCAATCT TGCAGTATCC ATATGATAAT GGTTATTATC TACCATACTA CAAGAGGGAG AGGAACAAAC GAAGCACACA GATTACAGTC AGATTCCTCG ACAACCCACA TTACAACAAG AATATCCGCA AAAAGGATCC TGTTCTTCTT TTACACTGGT GGAAAGAAAT AGTTGCAACG ATTTTGTTTT GTATCATAGC AACAACGTTT ATTGTGCGCA GGCTTTTCCA TCCTCATCCT CACAGGCAAA GGAAGGAGTC TGAAACTCAG TGTCAAACTG AAAATAAATA TGATTCTGTA AGTGGTGAAG CCAATGACAG TAGCTGGAAT GACATAAAAA ACTCTGGATA TATATCACGA TATCTAACTG ATTTTGAGCC AATTCAATGC CTGGGACGTG GTGGCTTTGG AGTTGTTTTT GAAGCTAAAA ACAAAGTAGA TGACTGCAAT TATGCTATCA AGAGGATCCG TCTCCCCAAT AGGGAATTGG CTCGGGAAAA GGTAATGCGA GAAGTTAAAG CCTTAGCCAA GCTTGAACAC CCGGGCATTG TTAGATATTT CAATGCCTGG CTCGAAGCAC CACCAGAGAA GTGGCAAGAA AAGATGGATG AAATTTGGCT GAAAGATGAA AGCACAGACT GGCCACTCAG CTCTCCTAGC CCAATGGATG CACCATCAGT TAAAATACGC AGAATGGATC CTTTCGCTAC AAAAGAACAT ATTGAAATCA TAGCTCCTTC ACCACAAAGA AGCAGGTCTT TTTCAGTAGG GATTTCCTGT GACCAGACAA GTTCATCTGA GAGCCAGTTC TCACCACTGG AATTCTCAGG AATGGACCAT GAGGACATCA GTGAGTCAGT GGATGCAGCA TACAACCTCC AGGACAGTTG CCTTACAGAC TGTGATGTGG AAGATGGGAC TATGGATGGC AATGATGAGG GGCACTCCTT TGAACTTTGT CCTTCTGAAG CTTCTCCTTA TGTAAGGTCA AGGGAGAGAA CCTCCTCTTC AATAGTATTT GAAGATTCTG GCTGTGATAA TGCTTCCAGT AAAGAAGAGC CGAAAACTAA TCGATTGCAT ATTGGCAACC ATTGTGCTAA TAAACTAACT GCTTTCAAGC CCACCAGTAG CAAATCTTCT TCTGAAGCTA CATTGTCTAT TTCTCCTCCA AGACCAACCA CTTTAAGTTT AGATCTCACT AAAAACACCA CAGAAAAACT CCAGCCCAGT TCACCAAAGG TGTATCTTTA CATTCAAATG CAGCTGTGCA GAAAAGAAAA CCTCAAAGAC TGGATGAATG GACGATGTAC CATAGAGGAG AGAGAGAGGA GCGTGTGTCT GCACATCTTC CTGCAGATCG CAGAGGCAGT GGAGTTTCTT CACAGTAAAG GACTGATGCA CAGGGACCTC AAGCCATCCA ACATATTCTT TACAATGGAT GATGTGGTCA AGGTTGGAGA CTTTGGGTTA GTGACTGCAA TGGACCAGGA TGAGGAAGAG CAGACGGTTC TGACCCCAAT GCCAGCTTAT GCCAGACACA CAGGACAAGT AGGGACCAAA CTGTATATGA GCCCAGAGCA GATTCATGGA AACAGCTATT CTCATAAAGT GGACATCTTT TCTTTAGGCC TGATTCTATT TGAATTGCTG TATCCATTCA GCACTCAGAT GGAGAGAGTC AGGACCTTAA CTGATGTAAG AAATCTCAAA TTTCCACCAT TATTTACTCA GAAATATCCT TGTGAGTACG TGATGGTTCA AGACATGCTC TCTCCATCCC CCATGGAACG ACCTGAAGCT ATAAACATCA TTGAAAATGC TGTATTTGAG GACTTGGACT TTCCAGGAAA AACAGTGCTC AGACAGAGGT CTCGCTCCTT GAGTTCATCG GGAACAAAAC ATTCAAGACA GTCCAACAAC TCCCATAGCC CTTTGCCAAG CAATTAGAAT CTAGAGCGGC CGCCGAATTC GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GATGAAAAAG CCTGAACTCA CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAGCAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
