pLV2-CMV-UBR5(human)-Myc-IRES-Puro
$171.00
In stock
SKU
PL53850
NM_015902.6;DD5; EDD; EDD1; HYD
100μg liquid plasmid(Instantly):$192
| name | pLV2-CMV-UBR5(human)-Myc-IRES-Puro | another name | apLV2-CMV-UBR5(human)-Myc-IRES-Puro |
| Insert Size (bp) | 8397bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC TACTAGAGGA TCTATTTCCG GTGAATTCGC CACCATGACG TCCATCCATT TCGTGGTTCA CCCGCTGCCG GGCACCGAGG ACCAGCTCAA TGACAGGTTA CGAGAAGTTT CTGAGAAGCT GAACAAATAT AATTTAAACA GCCACCCCCC TTTGAATGTA TTGGAACAGG CTACTATTAA ACAGTGTGTG GTGGGACCAA ATCATGCTGC CTTTCTTCTT GAGGATGGTA GAGTTTGCAG GATTGGTTTT TCAGTACAGC CAGACAGATT GGAATTGGGT AAACCTGATA ATAATGATGG GTCAAAGTTG AACAGCAACT CGGGGGCAGG GAGGACGTCA AGGCCTGGTA GGACAAGCGA CTCTCCATGG TTTCTCTCAG GTTCTGAGAC TCTAGGCAGG CTGGCAGGCA ACACCTTAGG AAGCCGCTGG AGTTCTGGAG TGGGTGGAAG TGGTGGAGGA TCCTCTGGTA GGTCATCAGC TGGAGCTCGA GATTCCCGCC GGCAGACTCG AGTTATTCGG ACAGGACGGG ATCGAGGGTC TGGGCTTTTG GGCAGTCAGC CCCAGCCAGT TATTCCAGCA TCTGTCATTC CAGAGGAGCT GATTTCACAG GCCCAAGTTG TTTTACAAGG CAAATCCAGA AGTGTCATTA TTCGAGAACT TCAGAGAACA AATCTTGATG TGAACCTTGC TGTAAATAAT TTACTTAGCC GGGATGATGA AGATGGAGAT GATGGGGATG ATACAGCCAG CGAATCTTAT TTGCCTGGAG AGGATCTTAT GTCTCTCCTT GATGCCGACA TTCATTCTGC CCACCCAAGT GTCATTATTG ATGCAGATGC CATGTTTTCT GAAGACATTA GCTATTTTGG TTACCCTTCT TTTCGTCGTT CATCACTTTC CAGGCTAGGC TCATCTCGAG TTCTCCTTCT TCCCTTAGAG AGAGACTCTG AGCTGTTGCG TGAACGTGAA TCCGTTTTAC GTTTACGTGA ACGAAGGTGG CTTGATGGAG CCTCATTTGA TAATGAAAGG GGTTCTACCA GCAAGGAAGG AGAGCCAAAC TTGGATAAGA AGAATACACC TGTTCAAAGT CCAGTATCTC TAGGAGAAGA TTTGCAGTGG TGGCCTGATA AGGATGGAAC AAAATTCATC TGTATTGGGG CTCTGTATTC TGAACTTCTG GCTGTCAGCA GTAAAGGAGA ACTTTATCAG TGGAAATGGA GTGAATCTGA GCCTTACAGA AATGCCCAGA ATCCTTCATT ACATCATCCA CGAGCAACAT TTTTGGGGTT AACCAATGAA AAGATAGTCC TCCTGTCTGC AAATAGCATA AGAGCAACTG TAGCTACAGA AAATAACAAG GTTGCTACAT GGGTGGATGA AACTTTAAGT TCTGTGGCTT CTAAATTAGA GCACACTGCT CAGACTTACT CTGAACTTCA AGGAGAGCGG ATAGTTTCTT TACATTGCTG TGCCCTTTAC ACCTGCGCTC AGCTGGAAAA CAGTTTATAT TGGTGGGGTG TAGTTCCTTT TAGTCAAAGG AAGAAAATGT TAGAGAAAGC TAGAGCAAAA AATAAAAAGC CTAAATCCAG TGCTGGTATT TCTTCAATGC CGAACATCAC TGTTGGTACC CAGGTATGCT TGAGAAATAA TCCTCTTTAT CATGCTGGAG CAGTTGCATT TTCAATTAGT GCTGGGATTC CTAAAGTTGG TGTCTTAATG GAGTCAGTTT GGAATATGAA TGACAGCTGT AGATTTCAAC TTAGATCTCC TGAAAGCTTG AAAAACATGG AAAAAGCTAG CAAAACTACT GAAGCTAAGC CTGAAAGTAA GCAGGAGCCA GTGAAAACAG AAATGGGTCC TCCACCATCT CCAGCATCCA CGTGTAGTGA TGCATCCTCA ATTGCCAGCA GTGCATCAAT GCCATACAAA CGACGACGGT CAACCCCTGC ACCAAAAGAA GAGGAAAAGG TGAATGAAGA GCAGTGGTCT CTTCGGGAAG TGGTTTTTGT GGAAGATGTC AAGAATGTTC CTGTTGGCAA GGTGCTAAAA GTAGATGGTG CCTATGTTGC TGTAAAATTT CCAGGAACCT CCAGTAATAC TAACTGTCAG AACAGCTCTG GTCCAGATGC TGACCCTTCT TCTCTCCTGC AGGATTGTAG GTTACTTAGA ATTGATGAAT TGCAGGTTGT CAAAACTGGT GGAACACCGA AGGTTCCCGA CTGTTTCCAA AGGACTCCTA AAAAGCTTTG TATACCTGAA AAAACAGAAA TATTAGCAGT GAATGTAGAT TCCAAAGGTG TTCATGCTGT TCTGAAGACT GGAAATTGGG TACGATACTG TATCTTTGAT CTTGCTACAG GAAAAGCAGA ACAGGAAAAT AATTTTCCTA CAAGCAGCAT TGCTTTCCTT GGTCAGAATG AGAGGAATGT AGCCATTTTC ACTGCTGGAC AGGAATCTCC CATTATTCTT CGAGATGGAA ATGGTACCAT CTACCCAATG GCCAAAGATT GCATGGGAGG AATAAGGGAT CCCGATTGGC TGGATCTTCC ACCTATTAGT AGTCTTGGAA TGGGTGTGCA TTCTTTAATA AATCTTCCTG CCAATTCAAC AATCAAAAAG AAAGCTGCTG TTATCATCAT GGCTGTAGAG AAACAAACCT TAATGCAACA CATTCTGCGC TGTGACTATG AGGCCTGTCG ACAATATCTA ATGAATCTTG AGCAAGCGGT TGTTTTAGAG CAGAATCTAC AGATGCTGCA GACATTCATC AGCCACAGAT GTGATGGAAA TCGAAATATT TTGCATGCTT GTGTATCAGT TTGCTTTCCA ACCAGCAATA AAGAAACTAA AGAAGAAGAG GAAGCGGAGC GTTCTGAAAG AAATACATTT GCAGAAAGGC TTTCTGCTGT TGAGGCCATT GCAAATGCAA TATCAGTTGT TTCAAGTAAT GGCCCAGGTA ATCGGGCTGG ATCATCAAGT AGCCGAAGTT TGAGATTACG GGAAATGATG AGACGTTCGT TGAGAGCAGC TGGTTTGGGT AGACATGAAG CTGGAGCTTC ATCCAGTGAC CACCAGGATC CAGTTTCACC CCCCATAGCT CCCCCTAGTT GGGTTCCTGA CCCTCCTGCG ATGGATCCTG ATGGTGACAT TGATTTTATC CTGGCCCCCG CTGTGGGATC TCTTACCACA GCAGCAACCG GTACTGGTCA AGGACCAAGC ACCTCCACTA TTCCAGGTCC TTCCACAGAG CCATCTGTAG TAGAATCCAA GGATCGAAAG GCGAATGCTC ATTTTATATT GAAATTGTTA TGTGACAGTG TGGTTCTCCA GCCCTATCTA CGAGAACTTC TTTCTGCCAA GGATGCAAGA GGGATGACCC CATTTATGTC AGCTGTAAGT GGCCGAGCTT ATCCTGCTGC AATTACCATC TTAGAAACTG CTCAGAAAAT TGCAAAAGCT GAAATATCCT CAAGTGAAAA AGAGGAAGAT GTATTCATGG GAATGGTTTG CCCATCAGGT ACCAACCCTG ATGACTCTCC TTTATATGTT TTATGTTGTA ATGACACTTG CAGTTTTACA TGGACTGGAG CAGAGCACAT TAACCAGGAT ATTTTTGAGT GTCGAACTTG TGGCTTGCTG GAGTCACTGT GTTGTTGTAC GGAATGTGCA AGGGTTTGTC ATAAAGGTCA TGATTGCAAA CTCAAACGGA CATCACCAAC AGCCTACTGT GATTGTTGGG AGAAATGTAA ATGTAAAACT CTTATTGCTG GACAGAAATC TGCTCGTCTT GATCTACTTT ATCGCCTGCT CACTGCTACT AATCTGGTTA CTCTGCCAAA CAGCAGGGGA GAGCACCTCT TACTATTCTT AGTACAGACA GTCGCAAGGC AGACGGTGGA GCATTGTCAA TACAGGCCAC CTCGAATCAG GGAAGATCGT AACCGAAAAA CAGCCAGTCC TGAAGATTCA GATATGCCAG ATCATGATTT AGAGCCTCCA AGATTTGCCC AGCTTGCATT GGAGCGTGTT CTACAGGACT GGAATGCCTT GAAATCTATG ATTATGTTTG GGTCGCAGGA GAATAAAGAC CCTCTTAGTG CCAGCAGTAG AATAGGCCAT CTTTTGCCAG AAGAGCAAGT ATACCTCAAT CAGCAAAGTG GCACAATTCG GCTGGACTGT TTCACTCATT GCCTTATAGT TAAGTGTACA GCAGATATTT TGCTTTTAGA TACTCTACTA GGTACACTAG TGAAAGAACT CCAAAACAAA TATACACCTG GACGTAGAGA AGAAGCTATT GCTGTGACAA TGAGGTTTCT ACGTTCAGTG GCAAGAGTTT TTGTTATTCT GAGTGTGGAA ATGGCTTCAT CCAAAAAGAA AAACAACTTT ATTCCACAGC CAATTGGAAA ATGCAAGCGT GTATTCCAAG CATTGCTACC TTACGCTGTG GAAGAATTGT GCAACGTAGC AGAGTCACTG ATTGTTCCTG TCAGAATGGG GATTGCTCGT CCAACTGCAC CATTTACCCT GGCTAGTACT AGCATAGATG CCATGCAGGG CAGTGAAGAA TTATTTTCAG TGGAACCACT ACCACCACGA CCATCATCTG ATCAGTCTAG CAGCTCCAGT CAGTCTCAGT CATCCTACAT CATCAGGAAT CCACAGCAGA GGCGCATCAG CCAGTCACAG CCCGTTCGGG GCAGAGATGA AGAACAGGAT GATATTGTTT CAGCAGATGT GGAAGAGGTT GAGGTGGTGG AGGGTGTGGC TGGAGAAGAG GATCATCATG ATGAACAGGA AGAACACGGG GAAGAAAATG CTGAGGCAGA GGGACAACAT GATGAGCATG ATGAAGACGG GAGTGATATG GAGCTGGACT TGTTAGCAGC AGCTGAAACA GAAAGTGATA GTGAAAGTAA CCACAGCAAC CAAGATAATG CTAGTGGGCG CAGAAGCGTT GTCACTGCAG CAACTGCTGG TTCAGAAGCA GGAGCAAGCA GTGTTCCTGC CTTCTTTTCT GAAGATGATT CTCAATCGAA TGACTCAAGT GATTCTGATA GCAGTAGTAG TCAGAGTGAC GACATAGAAC AGGAGACCTT TATGCTTGAT GAGCCATTAG AAAGAACCAC AAATAGCTCC CATGCCAATG GTGCTGCCCA AGCTCCCCGT TCAATGCAGT GGGCTGTCCG CAACACCCAG CATCAGCGAG CAGCCAGTAC AGCCCCTTCC AGTACATCTA CACCAGCAGC AAGTTCAGCG GGTTTGATTT ATATTGATCC TTCAAACTTA CGCCGGAGTG GTACCATCAG TACAAGTGCT GCAGCTGCAG CAGCTGCTTT GGAAGCTAGC AACGCCAGCA GTTACCTAAC ATCTGCAAGC AGTTTAGCCA GGGCTTACAG CATTGTCATT AGACAAATCT CGGACTTGAT GGGCCTTATT CCTAAGTATA ATCACCTAGT ATACTCTCAG ATTCCAGCAG CTGTGAAATT GACTTACCAA GATGCAGTAA ACTTACAGAA CTATGTAGAA GAAAAGCTTA TTCCCACTTG GAACTGGATG GTCAGTATTA TGGATTCTAC TGAAGCTCAA TTACGTTATG GTTCTGCATT AGCATCTGCT GGTGATCCTG GACATCCAAA TCATCCTCTT CACGCTTCTC AGAATTCAGC GAGAAGAGAG AGGATGACTG CGCGAGAAGA AGCTAGCTTA CGAACACTTG AAGGCAGACG ACGTGCCACC TTGCTTAGCG CCCGTCAAGG AATGATGTCT GCACGAGGAG ACTTCCTAAA TTATGCTCTG TCTCTAATGC GGTCTCATAA TGATGAGCAT TCTGATGTTC TTCCAGTTTT GGATGTTTGC TCATTGAAGC ATGTGGCATA TGTTTTTCAA GCACTTATAT ACTGGATTAA GGCAATGAAT CAGCAGACAA CATTGGATAC ACCTCAACTA GAACGCAAAA GGACGCGAGA ACTCTTGGAA CTGGGTATTG ATAATGAAGA TTCAGAACAT GAAAATGATG ATGACACCAA TCAAAGTGCT ACTTTGAATG ATAAGGATGA TGACTCTCTT CCTGCAGAAA CTGGCCAAAA CCATCCATTT TTCCGACGTT CAGACTCCAT GACATTCCTT GGGTGTATAC CCCCAAATCC ATTTGAAGTG CCTCTGGCTG AAGCCATCCC CTTGGCTGAT CAGCCACATC TGTTGCAGCC AAATGCTAGA AAGGAGGATC TTTTTGGCCG TCCAAGTCAG GGTCTTTATT CTTCATCTGC CAGTAGTGGG AAATGTTTAA TGGAGGTTAC AGTGGATAGA AACTGCCTAG AGGTTCTTCC AACAAAAATG TCTTATGCTG CCAATCTGAA AAATGTAATG AACATGCAAA ACCGGCAAAA AAAAGAAGGG GAAGAACAGC CCGTGCTGCC AGAAGAAACT GAGAGTTCAA AACCAGGGCC ATCTGCTCAT GATCTTGCTG CACAATTAAA AAGTAGCTTA CTAGCAGAAA TAGGACTTAC TGAAAGTGAA GGGCCACCTC TCACATCTTT CAGGCCACAG TGTAGCTTTA TGGGAATGGT TATTTCCCAT GATATGCTGC TAGGACGTTG GCGCCTTTCT TTAGAACTGT TCGGCAGGGT ATTCATGGAA GATGTTGGAG CAGAACCTGG ATCAATCCTA ACTGAATTGG GTGGTTTTGA GGTAAAAGAA TCAAAATTCC GCAGAGAAAT GGAAAAACTG AGAAACCAGC AGTCAAGAGA TTTGTCACTA GAGGTTGATC GGGATCGAGA TCTTCTCATT CAGCAGACTA TGAGGCAGCT TAACAATCAC TTTGGTCGAA GATGTGCTAC TACACCAATG GCTGTACACA GAGTAAAAGT CACATTTAAG GATGAGCCAG GAGAGGGCAG TGGTGTAGCA CGAAGTTTTT ATACAGCCAT TGCACAAGCA TTTTTATCAA ATGAAAAATT GCCAAATCTA GAGTGTATCC AAAATGCCAA CAAAGGCACC CACACAAGTT TAATGCAGAG ATTAAGGAAC CGAGGAGAGA GAGACCGGGA AAGGGAGAGA GAAAGGGAAA TGAGGAGGAG TAGTGGTTTG CGAGCAGGTT CTCGGAGGGA CCGGGATAGA GACTTTAGAA GACAGCTTTC CATCGACACT AGGCCCTTTA GACCAGCCTC TGAAGGGAAT CCTAGCGATG ATCCTGAGCC TTTGCCAGCA CATCGGCAGG CACTTGGAGA GAGGCTTTAT CCTCGTGTAC AAGCAATGCA ACCAGCATTT GCAAGTAAAA TCACTGGCAT GTTGTTGGAA TTATCCCCAG CTCAGCTGCT TCTCCTTCTA GCAAGTGAGG ATTCTCTGAG AGCAAGAGTG GATGAGGCCA TGGAACTCAT TATTGCACAT GGACGGGAAA ATGGAGCTGA TAGTATCCTG GATCTTGGAT TAGTAGACTC CTCAGAAAAG GTACAGCAGG AAAACCGAAA GCGCCATGGC TCTAGTCGAA GTGTAGTAGA TATGGATTTA GATGATACAG ATGATGGTGA TGACAATGCC CCTTTGTTTT ACCAACCTGG GAAAAGAGGA TTTTATACTC CAAGGCCTGG CAAGAACACA GAAGCAAGGT TGAATTGTTT CAGAAACATT GGCAGGATTC TTGGACTATG TCTGTTACAG AATGAACTAT GTCCTATCAC ATTGAATAGA CATGTAATTA AAGTATTGCT TGGTAGAAAA GTCAATTGGC ATGATTTTGC TTTTTTTGAT CCTGTAATGT ATGAGAGTTT GCGGCAACTA ATCCTCGCGT CTCAGAGTTC AGATGCTGAT GCTGTTTTCT CAGCAATGGA TTTGGCATTT GCAATTGACC TGTGTAAAGA AGAAGGTGGA GGACAGGTTG AACTCATTCC TAATGGTGTA AATATACCAG TCACTCCACA GAATGTATAT GAGTATGTGC GGAAATACGC AGAACACAGA ATGTTGGTAG TTGCAGAACA GCCCTTACAT GCAATGAGGA AAGGTCTACT AGATGTGCTT CCAAAAAATT CATTAGAAGA TTTAACGGCA GAAGATTTTA GGCTTTTGGT AAATGGCTGC GGTGAAGTCA ATGTGCAAAT GCTGATCAGT TTTACCTCTT TCAATGATGA ATCAGGAGAA AATGCTGAGA AGCTTCTGCA GTTCAAGCGT TGGTTCTGGT CAATAGTAGA GAAGATGAGC ATGACAGAAC GACAAGATCT TGTTTACTTT TGGACATCAA GCCCATCACT GCCAGCCAGT GAAGAAGGAT TCCAGCCTAT GCCCTCAATC ACAATAAGAC CACCAGATGA CCAACATCTT CCTACTGCAA ATACTTGCAT TTCTCGACTT TACGTCCCAC TCTATTCCTC TAAACAGATT CTCAAACAGA AATTGTTACT CGCCATTAAG ACCAAGAATT TTGGTTTTGT GGAGCAGAAA CTCATCTCTG AAGAGGATCT GTAGGGATCC CGCCCCTCTC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAAGCTT GCCACAACCC ACAAGGAGAC GACCTTCCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCC GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ACCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTAGAC GCGTCTGGAA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAATTAA TTCTGCAGTC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGA GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GCCTTGACAT TGCTAGCGTT TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCAACT GGATAACTCA AGCTAACCAA AATCATCCCA AACTTCCCAC CCCATACCCT ATTACCACTG CCAATTACCT GTGGTTTCAT TTACTCTAAA CCTGTGATTC CTCTGAATTA TTTTCATTTT AAAGAAATTG TATTTGTTAA ATATGTACTA CAAACTTAGT AGTTTTTAAA GAAATTGTAT TTGTTAAATA TGTACTACAA ACTTAGTAGT
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